MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yiran Cao,Jialin Li,Mengting Chen,Linyuan Zhou,Qiyue Zhang,Weipeng Liu,Yingju Liu
Hydrogels, as an extremely hydrophilic polymer with a network-like cross-linked structure, are considered as a promising material in the field of sensing and detection. In this work, a new ratiometric fluorescence sensor on the basis of the dual-emission of gold nanoclusters (AuNCs) and carbon dots (CDs) was developed to recognize copper ions (Cu 2+ ) and glutathione (GSH). AuNCs with excellent photostability and biocompatibility could electrostatically interact with aminated-CDs@SiO 2 . After adding Cu 2+ , the fluorescence of AuNCs gradually weakened, while the fluorescence of CDs in silicon spheres was basically not affected, thus a fluorescence ratio signal was generated to realize the recognition of Cu 2+ . Subsequently, the strong combination of GSH with Cu 2+ could recover the fluorescence of AuNCs, and a new fluorescence ratio signal was generated to further recognize GSH. The developed ratiometric fluorescence sensor could detect Cu 2+ over a range from 0.5 to 16 μM with a detection limit of 0.25 μM and GSH over a range from 0.05 to 48 μM with a detection limit of 0.035 μM. Such dual-emission CDs@SiO 2 /AuNCs fluorescent probe was located in the visible region, thus a multi-color fluorescent platform for the alternative visual recognition of Cu 2+ and GSH was realized by implanting CDs@SiO 2 /AuNCs in agar gel. The construction of multicolor fluorescent hydrogels can be not only applied to fast and visual on-site sensing, but also used in the bioimaging, information encoding, and other fields.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Fuyuan Huang,Paner Jiang,Yiliang Chen,Weiwei Wang,Yaxin Han,Chenlu Chen,Guangxin Xiang,Sheng Ye,Laibao Zheng
Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive approach for foodborne pathogen detection; however, a simple, user-friendly, and naked-eye readable readout method remains a challenge. Herein, we synthesized bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs), which exhibit fluorescence quenching upon copper ions (Cu 2+ ) binding and fluorescence recovery in the presence of phosphate ions (PPi). Leveraging this property, we developed a label-free fluorescence turn-on assay for naked-eye LAMP detection by monitoring PPi accumulation under UV light. To mitigate potential cross-contamination risks inherent in LAMP workflows, we integrated the BSA-AuNCs-Cu 2+ complex into the reaction lid, enabling a closed, one-pot detection system. This method demonstrated high sensitivity, detecting Salmonella enterica at concentrations as low as 10 1 CFU/mL within 40 min (excluding nucleic acid extraction process). With its customizable primers and naked-eye visual detection capability, this assay offers a versatile and practical tool for rapid pathogen screening in diverse applications.
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高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yue Cai,Hongshuai Zhu,Weichi Zhou,Ziyin Qiu,Congcong Chen,Aori Qileng,Kangshun Li,Yingju Liu
Organophosphorus pesticides (OPs) can inhibit the activity of acetylcholinesterase (AChE) to induce neurological diseases. It is significant to exploit a rapid and sensitive strategy to monitor OPs. Here, a metal–organic framework (MOF) acted as a carrier to encapsulate AuNCs, which can limit the molecular motion of AuNCs, trigger the aggregation-induced emission (AIE) effect, and exhibit a strong fluorescence with a fluorescence lifetime and quantum yield of 6.83 μs and 4.63%, respectively. Then, the marriage of fluorescence and colorimetric signals was realized on the basis of the dual function of the enzymolysis product from AChE and choline oxidase (CHO) on AuNCs@ZIF-8. First, it can decompose ZIF-8 to weaken the restraint on AuNCs, and thus the fluorescence receded. Second, it can be used as a substrate for the peroxidase mimics of the released AuNCs to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) and a visible blue appeared. Thus, on the basis of the inhibition of AChE activity by OPs, a fluorescence–colorimetric dual-signal biosensor was established. In addition, colorimetric paper strips were exploited to realize a visual semiquantitative detection, and a smartphone APP was developed to make the visualization results more precise and realize real-time supervision of pesticide contamination.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 11
·5971
·
IF 4.9
Yan-Xin Guo,Xin-Ran Guo,Xin-Yue Chen
A dual-emission ratio-fluorescent sensing nanohybrid based on RadixHedysarigreen-synthesized carbon quantum dots (CDs) and glutathione-functionalized gold nanoclusters (GSH-AuNCs) had been developed for the determination of cefodizime sodium (CDZM). The designed fluorescence nanohybrid had two significant fluorescence emission peaks at 458 nm and 569 nm when excited at 360 nm, which was attributed to the CDs and GSH-AuNCs. With the addition of CDZM, the fluorescence at 458 nm was slightly weakened while the fluorescence at 569 nm was enhanced obviously. Based on the relationship between the I569/I458fluorescence intensity ratio and the concentration of CDZM, the designed nanohybrid exhibited a good linearity range of 1.0–1000.0 μM and the limit of detection (LOD) was 0.19 μM. The method was finally applied in the detection of CDZM in urine, showing the potential applications in complicated biological samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xiaolun Peng,Jiaxin Zheng,Ting Bao,Wei Wen,Xiuhua Zhang,Shengfu Wang
A novel dual-amplified electrochemical biosensor based on Exonuclease III (Exo III) and Cu(II)-dependent DNAzymes (Cu 2+ -DNAzymes) was developed for ultrasensitive electrochemical evaluation of pyrophosphatase (PPase) activity with the help of one-step synthesis of BSA-directed gold nanoclusters-manganese dioxide (AuNCs-MnO 2 ) nanoflakes with peroxidase-like characteristics as a catalytic platform. For biosensor preparation, under the catalytic hydrolysis of the PPase, copper ion (Cu 2+ ) was released from the Cu 2+ -coordinated pyrophosphate ions (Cu 2+ –PPi) complex which was formed by the coordination reaction of copper ion (Cu 2+ ) and pyrophosphate ions (PPi). The released Cu 2+ , acting as an indispensable cofactor, could activate Cu 2+ -DNAzymes to turn on the amplification process and then generated the single-stranded product (S1) that hybridized to the hairpin-type capture probe (HP1). Subsequently, the addition of Exonuclease III (Exo III) enabled the reuse of S1 and produced the final cleavage fragment, which closely linked the functionalized AuNCs-MnO 2 nanoflakes to the electrode surface. The superior peroxidase-like characteristics of nanoflakes provided stable catalytic signal output and realized electrochemical monitoring of PPase activity with a detection limit of 0.0058 mU/mL. Importantly, the biosensor also had a good performance in inhibitors screening of target PPase. The use of AuNCs-MnO 2 nanoflakes instead of natural enzymes combined with DNAzymes amplification strategy offered more possibilities for the detection of various biomolecules.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-12
·
IF 6.232
Dong Wenjuan,Wang Ruiping,Gong Xiaojuan,Liang Wenting,Fan Li,Song Shengmei,Dong Chuan
A far-red fluorescence “off-on” sensing strategy is described for sequential ratiometric determination of Cu 2+ and L-histidine (L-His) based on fluorescence resonance energy transfer (FRET) system. N,S,P co-doped carbon dots (N,S,P-CDs) and N-acetyl-L-cysteine functionalized gold nanoclusters (NAC-AuNCs) are used in the FRET system, which serve as energy donor and acceptor, respectively. After adding NAC-AuNCs into the solution of N,S,P-CDs, the fluorescence of N,S,P-CDs is effectively quenched, while the far-red fluorescence of NAC-AuNCs appears. Cu 2+ can decrease fluorescence of NAC-AuNCs, and then L-His can effectively recover the fluorescence of NAC-AuNCs. The possible reason is that the stronger affinity between Cu 2+ and L-His can pull Cu 2+ away from the surface of NAC-AuNCs. Through it all, the emission intensity of N,S,P-CDs remains nearly constant, so the ratio of fluorescence intensities at 485 and 625 nm exhibits a linear correlation to the Cu 2+ and L-His concentration, respectively. The sensing platform shows good selectivity towards Cu 2+ and L-His with a linear range of 0.65–26.58 μM and 3.13–56.25 μM and determination limits of 0.50 μM and 0.374 μM, respectively. The proposed method has been successfully used for Cu 2+ and L-His determination in real samples with satisfying results.
Graphical abstract
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