Analytical Methods
·
2022
·Vol. 14
·期 45
·4669-4679
·
IF 3.532
Lvcheng Liu,Yuqi Ling,Junshan Han,Tingting Hao,Xing Li
Solvated mercuric ions (Hg2+), a toxic and harmful water pollutant, can easily accumulate in organisms and cause serious damage to the kidney, liver, and central nervous system. To realize rapid and efficient detection of mercury (II) ions in water sources, a kind of new colorimetric sensor of gold nanoparticles (AuNPs) functionalized with ribavirin (Rib-AuNPs) was proposed and characterized by TEM, DLS, XRD, and UV-vis in this work. The color of the Rib-AuNP solution rapidly changed from wine-red to gray-blue with the addition of Hg2+ based on the aggregation mechanism. The limits of detection (LODs) are 0.20 μM by the naked eye and 3.64 nM by UV-vis spectroscopy with a fine linear relationship in the range of 0–0.25 μM (R2 = 0.9834) and 0.25–0.80 μM (R2 = 0.9893) of Hg2+, indicating that the detection system of Rib-AuNPs could be applied to analyze Hg2+ with excellent selectivity and anti-interference in real water samples.
研究领域:
环境科学
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分析化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Rentang Huang,Yuxin Huang,Hongmei Liu,Kaiyi Guan,Ali Chen,Xiangsheng Zhao,Shumei Wang,Lei Zhang
Herein, we present a bifunctional gold nanoparticles (AuNP) probe-based enzyme-linked immunosorbent assay (nano-ELISA) for detecting zearalenone (ZEN) in the popular edible and medicinal food coix seed. This nano-ELISA takes advantage of the convenient labeling property of AuNPs, which allows the horseradish peroxidase and antibody were successively immobilized onto AuNPs by electrostatic adsorption to construct a bifunctional probe for identification and signal amplification. In our model system, a time-saving direct competitive ELISA scheme was selected, in which mutual optimization of biomolecules, a two-stage process for incubation, and comparison of different particle sizes were performed to reach higher sensitivity. The proposed method achieves a limit of detection of 8 μg/kg and a limit of quantitation of 22 μg/kg, which is an approximately 7-fold and 5-fold improvement over the traditional ELISA, respectively. The calibration curve for the analyte was linear over the concentration range of 22–508 μg/kg. The recoveries for ZEN spiked coix seed samples ranged from 98.67 to 113.5 % with a relative standard deviation of less than 8 %. Moreover, the developed nano-ELISA could overcome the interference from the sample matrix. A good correlation was observed between the results from nano-ELISA and liquid chromatography-tandem mass spectrometry (r 2 = 0.9757, n = 69), which paves the useful and practicable way for accurate bioanalysis for trace hazardous substances in food.
研究领域:
食品安全检测
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生物分析化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yuanyuan Yang,Xiaohui Liu,Shuang Meng,Shun Mao,Wenquan Tao,Zhuo Li
A molecularly imprinted polymers (MIPs)-isolated AuNP-enhanced fluorescence sensor, AuNP@MIPs-CdTe QDs, was developed for highly sensitive and selective detection of oxytetracycline (OTC) in aqueous medium. The developed sensor combined the advantages of strong fluorescence signal of metal-enhanced fluorescence (MEF), high selectivity of MIPs, and stability of CdTe QDs. The MIPs shell with specific recognition served as an isolation layer to adjust the distance between AuNP and CdTe QDs to optimize the MEF system. The sensor demonstrated the detection limit as low as 5.22 nM (2.40 μg/L) for a concentration range of 0.1–3.0 μM OTC and good recovery rates of 96.0–103.0% in real water samples. In addition, high specificity recognition for OTC over its analogs was achieved with an imprinting factor of 6.10. Molecular dynamics (MD) simulation was utilized to simulate the polymerization process of MIPs and revealed H-bond formation as the mainly binding sites of APTES and OTC, and finite-difference time-domain (FDTD) analysis was employed to obtain the distribution of electromagnetic field (EM) for AuNP@MIPs-CdTe QDs. The experimental results combined with theoretical analyses not only provided a novel MIP-isolated MEF sensor with excellent detection performance for OTC but also established a theoretical basis for the development of a new generation of sensors.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2023
·
IF 4.1
Junyou Li,Hung-Wing Li
By combining exquisitely designed hairpins with the catalytic hairpin assembly to form tripedal DNA walkers driven by enzyme, we constructed a 3D DNA walker with accordingly complementary hairpins attached on gold nanoparticles (AuNPs) and sensitive fluorescence sensing system for the sensitive detection of target miRNA-21. The presence of miR-21 triggers the CHA among three hairpins, which lead to the formation of the tripedal DNA walkers. For the walking trajectories, FAM-labeled hairpins were attached to the surface of AuNPs, the fluorescence of which was initially quenched due to its close proximity to AuNPs. After the binding/cleaving/moving process of tripedal DNA walkers with HP4 driven by Exonuclease III (Exo III), a number of single-stranded DNAs (ssDNAs) will be released with FAM fluorescence recovered. Benefiting from the DNA walker and CHA cascade amplification, the proposed sensing strategy showed remarkable improvement in sensitivity with the LOD of 46 aM. Also, the system was practically applied in human serum samples, demonstrating satisfactory outcome. Owing to the precise design of the system, this method exhibited excellent specificity to distinguish miR-21 from its single-, double-mismatched sequences and non-complementary sequences, showing great versatility and potential for the biological analysis and early disease diagnosis.
研究领域:
生物技术
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纳米技术
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癌症诊断
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-8
·
IF 5.479
Liu Xinyan,Deng Keqin,Wang Hao,Li Chunxiang,Zhang Shaowei,Huang Haowen
Gold nanoparticles (AuNPs) with a size of ~3 nm were placed on a thionine-multiwalled carbon nanotube (Thi-CNTs) conjugate to form a novel AuNP-Thi-CNTs nanocomposite. Its morphology and composition were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The nanocomposite was placed on an electrode and used as a redox-active signaling interface to fabricate a ratiometric electrochemical aptasensor for 17β-estradiol (E2). The potentiostatic insertion method was applied to insert an aptamer against E2 into a thin alkane monolayer to warrant an adequate distance between aptamers. The aptamer against E2 acts as both a collector and separator to specifically bind E2. The electrode displays two peak signals (at +0.50 V vs. SCE for E2; and at −0.32 V for Thi) which increase and decrease, respectively, in the 12 pM to 60 nM E2 concentration range. Therefore, the current ratio can be used to reliably, reproducibly, and sensitively quantify the concentration of E2.
Schematic presentation of a novel AuNP-Thi-CNTs nanocomposite. AuNP-Thi-CNTs showed good electrocatalytic oxidation to E2. AuNP-Thi-CNTs was used as self-redox signal interface to fabricate aptasensor. Dual signals of extrinsic E2 and inner Thi was applied to monitor the concentration of E2.
研究领域:
分析化学
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纳米技术
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电化学
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生物传感器
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jidong Tang,Ling Wang,Wenjin Ma,Ping Fu,Yanlin Zhang,Xiaoqiong Li,Xiaoqi Tao
The application of the fluorescence resonance energy transfer (FRET) strategy in the detection of ochratoxin A (OTA) is restricted by factors such as the photobleaching of fluorescent dyes, the autofluorescence of ochratoxin A (OTA), and the necessity for excitation by an external light source. The chemiluminescence resonance energy transfer (CRET) technique provides a more effective solution to this issue. In this paper, we developed a novel CRET aptasensor, based on gold nanoparticle (AuNP)-mediated CRET and Nb.BbvCI-powered DNA walker signal amplification. In the AuNPs/Capture-DNA/HRP probe, horseradish peroxidase (HRP) approached the AuNP surface, resulting in CRET. AuNPs quenched the chemiluminescence generated by HRP-catalyzed luminol. The presence of OTA induced the separation of the Walk-DNA from the aptamer and the formation of a double strand with Capture-DNA. This activated Nb.BbvCI to cleave Capture-DNA, releasing HRP and Walk-DNA. The Walk-DNA acted as a DNA walker, moving toward the adjacent Capture-DNA and initiating the next cleavage by Nb.BbvCI. Ultimately, a significant amount of HRP was released from the AuNP surface, weakening the CRET effect while generating a strong chemiluminescent signal. The CRET aptasensor had a detection limit of 2.75 nM with a linear range of 5–40 nM and was applied to detect OTA in glutinous rice.
研究领域:
食品科学
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生物传感
·
分析化学
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产品关联
DOI
高影响因子
ACS Applied Electronic Materials
·
2025
·
IF 4.7
Xinyu Liu,Long Yu,Shengyu Wang,Hanyu Cai,Xuqin Ran,Jinlin Xie,Zhenfei Guo,Kunlin Chen,Haoxuan Li,Peng Gu
Electric heating textiles not only can be used as commercial electric heating products but also have great potential in the application of medical fields. In this work, a Ti3C2Tx (MXene) and gold nanoparticle (AuNP)-decorated carbon nanotube yarn (CNTY) for one-dimensional (1D) textile heaters was fabricated through an in situ self-reduction reaction of MXene in HAuCl4 aqueous solution to improve the electrical properties of the carbon nanotube yarns. Based on the excellent conductivity of the MXene/AuNP-decorated CNTYs, this 1D textile heater exhibits excellent electro-thermal properties with good wash stability and acid and alkali resistance. At a voltage of 3 V, the saturation temperature can quickly reach ∼154 °C. After integration with conventional fabrics, the devices exhibit excellent thermal response and cycling stability, reaching ∼92 °C at a safe voltage (6 V) and remaining stable for over 1000 s.
研究领域:
材料科学
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纳米技术
·
纺织工程
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产品关联
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 13
·3134-3140
·
IF 3.864
Xiaotong Wei,Xiaolei Duan,Xiaoyan Zhou,Jiangling Wu,Hongbing Xu,Xun Min,Shijia Ding
Herein, a dual channel surface plasmon resonance imaging (SPRi) biosensor has been developed for the simultaneous and highly sensitive detection of multiplex miRNAs based on strand displacement amplification (SDA) and DNA-functionalized AuNP signal enhancement. In the presence of target miRNAs (miR-21 or miR-192), the miRNAs could specifically hybridize with the corresponding hairpin probes (H) and initiate the SDA, resulting in massive triggers. Subsequently, the two parts of the released triggers could hybridize with capture probes (CP) and DNA-functionalized AuNPs, assembling DNA sandwiches with great mass on the chip surface. A significantly amplified SPR signal readout was achieved. This established biosensing method was capable of simultaneously detecting multiplex miRNAs with a limit of detection down to 0.15 pM for miR-21 and 0.22 pM for miR-192. This method exhibited good specificity and acceptable reproducibility. Moreover, the developed method was applied to the determination of target miRNAs in a complex matrix. Thus, this developed SPRi biosensing method may present a potential alternative tool for miRNA detection in biomedical research and clinical diagnosis.
研究领域:
生物传感技术
·
分子诊断
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分析化学
·
医学检测
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 38
·18124-18130
·
IF 3.591
Baolei Liu,Yutian Su,Shishan Wu,Jian Shen
Chronic bacteria infected wound repair poses a critical threat due to the deteriorating environment of the wound. Herein, with the reducibility of black phosphorus nanosheets (BPs), we designed two-dimensional antibacterial BP@AuNP nanocomposites by combining gold nanoparticles (AuNPs) with BPs, which possess photothermal properties and high singlet oxygen (1O2) production under 650 nm light, respectively. The BP@AuNP nanocomposites use a simple method to overcome the defects of BPs that require two kinds of light sources to perform photothermal and photodynamic therapies simultaneously and exhibited synergistic combination of multiple therapies for S. aureus and E. coli under a 650 nm laser. The antibacterial properties in vivo of the nanocomposites were also investigated and BPs@AuNPs possessed a fast wound closure rate. Thus, our BP@AuNP nanocomposites expand the application of BPs and demonstrate a versatile strategy for infected wound healing.
研究领域:
生物医学工程
·
纳米医学
·
感染与免疫
✓ 已验证
DOI
高影响因子
Advanced Materials Interfaces
·
2018
·Vol. 5
·期 22
·1801070
·
IF 4.834
Lin Fan,Doudou Lou,Haoan Wu,Xizhi Zhang,Yefei Zhu,Ning Gu,Yu Zhang
In spite of the competitive advantages, inorganic nanoparticle mimic enzymes exhibit inherent disadvantages of limited catalytic efficiency and lacking selectivity. Here, a gold nanoparticle (AuNP)-based mimic enzyme with significantly enhanced glucose selectivity and catalytic activity is constructed and demonstrated for the first time. Aminophenylboronic acid is employed to increase the affinity to glucose, as well as build molecular imprinted polymer shells to realize the selectivity for template molecules of glucose. Besides that, heptadecafluoro- n -octyl bromide nanoemulsion with the function of providing oxygen is introduced to gain a further improvement in catalytic activity, which successfully enhances the catalytic efficiency ( k cat / K m ) up to about 270-fold. Based on the demonstrated catalytic properties, AuNP-based glucose oxidase mimics are successfully applied in practical glucose detection of drinks and blood glucose.
研究领域:
纳米技术
·
生物化学
·
催化
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2024
·
IF 4.6
Yu Huang,Xiaomei Li,Hua Zhang,Zhiguo Wu,Rohan Weerasooriya,Xing Chen,Jiu Zhou,Jiangling Wu,Jianjiang Xue,Junbo Wang,Lei Feng
A simple and low-cost fabrication method via polydopamine accelerated electroless plating (ELP) for an optical fiber surface plasmon resonance (SPR) sensor with molybdenum disulfide nanosheets (MoS 2 NSs) and gold nanoparticles (AuNPs) hybrid structure was proposed. The MoS 2 NSs were prepared by ultrasonic-assisted mixed-solvent liquid-phase exfoliation method. MoS 2 NSs and AuNPs were sequentially immobilized on the optical fiber by electrostatic interaction and Au-S bond supported by polydopamine and 1,2-ethanedithiol respectively, and a gold film supporting SPR was fabricated by ELP to form the optical fiber SPR sensor. The synthesized nanostructures and optical fiber sensors were characterized for their structural, morphological and optical properties. Some key experimental parameters, including dopamine concentration, dopamine polymerization temperature, dopamine polymerization time, pH of MoS 2 NSs solution and plating time, were investigated in detail. The refractive index sensitivity of sensor with the fiber/MoS 2 /AuNPs/Au structure achieved 678.85–7231.64 nm/RIU in the range of 1.3329–1.3638, which was approximately 52.35 % and 16.37 % higher than that of conventional sensors in the low refractive index range and high refractive index range respectively. This work proposed a facile, rapid and effective method to fabricate optical fiber SPR sensor to enhance its sensitivity, which indicates the potential application of the fabricated sensor in optical sensing field.
研究领域:
材料科学
·
光学传感
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Hong Zhang,Zhigao Zhang,Longyang Han,Mingqin Zhao,Pengfei Liu,Zhen Luo
Aflatoxin B 1 (AFB 1 ) has seriously threatened human health, so it is vital to construct an efficient detection strategy for AFB 1 analysis. An exquisitely designed ratiometric electrochemical aptasensor for sensitive detection of AFB 1 has been successfully developed in this work. DNA tetrahedral scaffold (DTS) is used to fix the DNA probes, 3D DNAzyme walker is employed to amplify the detection signals, and the composite material AuNP/ZIF-67/g-C 3 N 4 acts as the sensing surface of the detection electrode. In the presence of AFB 1 , the 3D DNAzyme walker induced the release of the oligonucleotide attached ferrocene (Fc) from the detection electrode and is simultaneously grafted the DNA-modified methylene blue (MB) onto the electrode, leading to a seesaw-like ratiometric electrochemical aptasensor. This ratiometric electrochemical aptasensor provides an outstanding AFB 1 analysis in the linear range of 2.0 fg·mL −1 to 1.0 ng·mL −1 with the limit of detection (LOD) 0.67 fg·mL −1 . It provides new idea for the analysis of other contaminants in foodstuffs.
研究领域:
生物技术
·
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）