Biosensors-Basel
·
2022
·Vol. 12
·期 7
·513
·
IF 5.743
Xingyue Chen,Lulan Yang,Jiaming Tang,Xu Wen,Xiaoling Zheng,Lingling Chen,Jiaqi Li,Yong Xie,Tao Le
Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (Kd= 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25–160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1–100.1% and coefficients of variation (CVs) of 2.2–10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.Keywords:molecular docking;molecular dynamics simulations;aptamer truncation;fluorescence aptasensor;gold nanoparticles
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Xie Dan,Wang Ruimeng,Fu Jinghao,Zhao Zhongxing,Li Min
Surface-enhanced Raman scattering (SERS) is an important and powerful analytical technique in chemical and biochemical analyses. Metal–organic frameworks (MOFs) can effectively capture volatile organic compounds (VOCs) with high adsorption capacity and fast kinetics, and the local surface plasmon resonance characteristics of gold nanoparticles can quickly and effectively distinguish different VOCs by SERS. Combining both, we designed a novel SERS substrate based on embedding gold nanoparticles (AuNPs) within MIL-101(Cr) for the recognition of various VOCs in the gaseous phase. Occupying of AuNPs inside MIL-101(Cr) increased the micropore-specific surface area of AuNPs@MIL-101(Cr), which enabled AuNPs@MIL-101(Cr) to absorb more toluene molecules and consequently realized its high detection sensitivity. The detection limits for toluene, 4-ethylbenzaldehyde, and formaldehyde were down to 6, 5, and 75, ppm respectively. Moreover, this substrate could be used for detecting different VOCs simultaneously. Finally, we discussed the enhancement of AuNPs outside and inside MIL-101(Cr) on the Raman signal.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 45
·20838-20845
·
IF 3.3
Wenli Hou,Xianyu Kang,Jiajie Lin,Mengying Xie,Yancai Li
In this work, bagasse derived nitrogen-doped activated carbon (B-dNAC) was prepared using waste sugarcane as a carbon precursor via a KOH-urea activated method. Then, gold nanoparticles were successfully loaded onto the B-dNAC and gold nanoparticles/B-dNAC nanocomposites (AuNPs/B-dNACNs) were obtained through the incorporation of chloroauric acid by a facile thermal reduction process. The structural composition and the morphology of the composites were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). A AuNPs/B-dNACNs modified glassy carbon electrode (AuNPs/B-dNACNs/GCE) was constructed. The electrochemical behavior of 4-aminophenol (4-AP) on the AuNPs/B-dNACNs/GCE was investigated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The AuNPs/B-dNACNs/GCE exhibited good electrocatalytic performance for the electrooxidation of 4-AP and can be used as a novel electrochemical sensor for the detection of 4-AP. Under optimal conditions, the linear range of 4-AP was 1–400 μM, with a low detection limit of 0.61 μM. In addition, it displayed good selectivity, reproducibility and stability. Most importantly, the AuNPs/B-dNACNs/GCE could be used for the determination of 4-AP in acetaminophen tablets, and good recovery was obtained in the range of 97.1%–104.9%.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Na Wu,Yi-Ting Wang,Xiao-Yan Wang,Feng-Na Guo,Hui Wen,Ting Yang,Jian-Hua Wang
Nanozymes have emerged as promising alternatives to overcome the high cost and low stability issues of natural enzymes . Particularly, those with peroxidase-like activities have been extensively studied to construct versatile biosensors . In this article, we demonstrate that the modification of the graphitic carbon nitride nanosheets (g-C 3 N 4 nanosheets) by plasmonic gold nanoparticles (AuNPs) greatly enhances its catalytic performance as peroxidase mimetic. In the presence of H 2 O 2 , the [email protected] 3 N 4 nanosheets can catalyze the redox reaction of 3,3′,5,5′- tetramethylbenzidine (TMB) to produce a blue color. Based on the observation, a colorimetric sensing method for glucose is further developed with the assistance of glucose oxidase (GOx). The linear range for glucose is from 5 to 100 μmol L −1 (R 2 = 0.9967) and the limit of detection (LOD) is 1.2 μmol L −1 . The LOD can be further lowered down to 0.75 μmol L −1 by using H 2 SO 4 as termination agent and measuring the absorbance of the yellow product at λ = 451 nm. Moreover, the practical usefulness of [email protected] 3 N 4 nanosheets as a peroxidase nanozyme for glucose determination in human serum and urine is also demonstrated.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yujie Han,Ran Zhang,Chuan Dong,Fangqin Cheng,Yujing Guo
Nitrite ions (NO 2 − ) have been widely used in the food and drink industry as preservatives. However, the NO 2 − discharged into the environment is harmful to the ecosystem and human health. Due to its potential toxicity, selective and sensitive detection of nitrite is important. In this work, a rose-like Au nanoparticles/MoS 2 nanoflower/graphene (AuNPs/MoS 2 /GN) composite was fabricated using a one-pot hydrothermal method without the addition of any extra reductant for use in nitrite detection. Graphene acts as an efficient matrix for the growth of MoS 2 nanoflower (NF), and the edges of the MoS 2 NF subsequently load AuNPs. The obtained AuNPs/MoS 2 /GN composite exhibits excellent electrooxidative activity toward nitrite ions, which is attributable to its large specific surface area, good conductivity, and the synergistic catalysis of each component. Accordingly, we propose a rapid, sensitive, and cost-effective electrochemical method for nitrite detection, which achieved a linear dynamic range of 5.0 μM to 5.0 mM with a detection limit of 1.0 μM. The present work provides not only a general one-pot synthesis method for a variety of noble–transition metal dichalcogenides nanohybrids , but also an example of the fabrication of an electrochemical nitrite sensor using a nanohybrid as an enhanced material, an approach that can easily be extended to other sensors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xuexue Ma,Chen Fang,Jilin Yan,Qun Zhao,Yifeng Tu
In this paper, we will describe a novel lable-free electrochemiluminescent (ECL) immunosensor for the detection of glutamate decarboxylase antibody (GADA) in which Au nanoparticles (AuNPs) had pre-functionalized the indium tin oxide (ITO) glass to support the sensing interface. The preparation of the basal electrode only need a simple two-step drop coating, a thin polymer of hydrolyzed 3-aminopropyl trimethoxysilane, and the AuNPs gel on the ITO substrate. The AuNPs not only enhanced the ECL signal of luminol, but also acted to immobilize the glutamate decarboxylase (GAD) to build the sensing host. This immunosensor exhibits excellent specificity, reproducibility and stability. On resultant sensor, after the direct immunoreaction, the decreased ECL intensity has a good linear regression toward the logarithm of GADA concentration in the range of 0.30 ng mL −1 to 50 ng mL −1 with a detection limit of 0.10 ng mL −1 . The proposed senor has prospective capability for the clinical detection of GADA in human serums, which had important value for diagnosis and precaution of type-1 diabetes or latent autoimmune diabetes in adult (LADA).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 12
·963
·
IF 3.504
Wei Liu,Xiaolian Zhu,Chengcheng Xu,Zhao Dai,Zhaohui Meng
A new AuNPs-based thermosensitive nanoreactor (SiO2@PMBA@Au@PNIPAM) was designed and prepared by stabilizing AuNPs in the layer of poly(N,N’-methylenebisacrylamide) (PMBA) and subsequent wrapping with the temperature-sensitive poly(N-isopropylacrylamide) (PNIPAM) layer. The new nanoreactor exhibited high dispersibility and stability in aqueous solution and effectively prevented the aggregation of AuNPs caused by the phase transformation of PNIPAM. The XPS and ATR-FTIR results indicated that AuNPs could be well stabilized by PMBA due to the electron transfer between the N atoms of amide groups in the PMBA and Au atoms of AuNPs. The catalytic activity and thermoresponsive property of the new nanoreactor were invested by the reduction of the environmental pollutant, 4-nitrophenol (4-NP), with NaBH4as a reductant. It exhibited a higher catalytic activity at 20 °C and 30 °C (below LCST of PNIPAM), but an inhibited catalytic activity at 40 °C (above LCST of PNIPAM). The PNIPAM layer played a switching role in controlling the catalytic rate by altering the reaction temperature. In addition, this nanoreactor showed an easily recyclable property due to the existence of a silica core and also preserved a rather high catalytic efficiency after 16 times of recycling.Keywords:nanoreactor;distillation precipitation polymerization;catalytic reduction;thermosensitive;AuNPs
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 12
·2156-2161
·
IF 5.068
Liu Yuan,Deng Yan,Li Taotao,Chen Zhu,Chen Hui,Li Song,Liu Hongna
A mercury ion aptamer electrochemical biosensor based on a Thymine-Hg2+-Thymine (T-Hg2+-T) structure has been constructed and successfully used to detect mercury ions in tap water samples. The aptamer electrochemical biosensor was assembled using a mercury ion
aptamer-functionalized AuNPs-modified glass carbon electrode (aptamer/(AuNPs/CS)2/GCE) capable of specifically detecting mercury ions through a T-Hg2+-T structure. The experimental results indicated the optimum electrochemical performance of the prepared aptamer biosensor
when the (AuNPs/CS)2/GCE surface was modified with 1.0 μM aptamer and incubated with Hg2+ for 60 min. Moreover, the aptamer biosensor exhibits a good linear relationship between the logarithm of the Hg2+ concentration and the DPV peak current in the
range from 0.01 to 500 nM following the linearization equation Ip
(μA) = 2.59902 + 0.2097logC (R
2 = 0.9994) with a limit of detection as low as 0.005 nM. Therefore, the constructed aptamer biosensor provides a simple and sensitive approach
for Hg2+ detection in aqueous solution with promising application for trace Hg2+ detection in real samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 29
·22792-22801
·
IF 3.84
Jun Deng,Honghao Zheng,Sai Wu,Pan Zhang,Changyou Gao
The chain length of ligands plays an important role in many biomedical applications. Yet the interaction of nanoparticles with surface-capped ligands of different length (especial the hydrophobic chains) and cells is neglected, and thereby the underlying mechanism influencing cellular uptake is unclear. Herein the role of alkyl length on AuNPs in mediating serum protein adsorption and the subsequent cellular uptake by A549 cells was studied. The AuNPs were modified with carboxylic alkyl thiols of different chain length (3MA, 11MA and 16MA), resulting in MA-AuNPs with a size of 5 nm in dry state and several tens of nm in media. The MA-AuNPs possessed a negatively charged surface, whose zeta potential was around −20 mV in serum-containing medium regardless of the length of ligands. Whereas the total amount of adsorbed serum proteins had no significant difference, the relative amounts of adhesion mediators especially vitronectin (Vn) depended on the alkyl length significantly. The 16MA-AuNPs adsorbed the largest amount of Vn, and were internalized by A549 cells with the largest quantity and good distribution in cytoplasm. Taking account all the results, the protein adsorption and its role in linking alkyl length on AuNPs to cellular uptake are figured out.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yalin Xie,Liping Chen,Kaixin Cui,Yu Zeng,Xiaojun Luo,Xiaojun Deng
As we all know, SERS (Surface-enhanced Raman spectroscopy) is widely used in sensing, analysis and detection. The covalent organic frameworks (COFs) have performed well as a material for supporting metal nanoparticles and facilitating analyte adsorption in SERS, which may greatly enhance the detection sensitivity and reproducibility. The synthesis of traditional metal/COFs composites involved chemical reduction methods, however, the resulting metallic NPs exhibited reduced capacity to enhance SERS due to their small particle sizes (usually <20 nm). This paper presented a novel photoreduction method for the facile growth of AuNPs (diameters: 75 nm) on COFs matrix under light control, which represents the first report of such synthesis on COF. Subsequently, the photoreduction deposition induced AuNPs/COFs composites, which served as highly sensitive and reproducible SERS-active substrates for capturing the spectral information of four types of macrolide antibiotics . The detection limits for the four macrolide antibiotics were determined to be 3.30 × 10 −11 , 3.43 × 10 −10 , 1.10 × 10 −10 and 5.78 × 10 −11 M, respectively, exhibiting excellent linear relationships within the concentration range of 10 −10 to 10 −3 M. Therefore, our proposed SERS method opens up a new idea for the development of SERS substrates and environmental safety monitoring, and it has great potential for ensuring food safety in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2024
·Vol. 32
·期 24
·42558-42568
·
IF 3.2
Lixia Li,Mingdeng Jin,Siyuan Wu,Ning Feng,Yufang Liu,Yufang Liu
Efficient and low-concentration detection of heavy metal ions is crucial for healthcare and environmental monitoring. Traditional fiber optic surface plasmon resonance (SPR) sensors face challenges in detecting trace heavy metal ions due to limited sensitivity and the need for complex specific modifications. To overcome these challenges, an innovative electrochemical and optical dual-mode fiber optic sensor for in situ, real-time detection of trace mercury ions is proposed in this paper. The sensor utilizes a reflection-type fiber optic probe coated with thin gold (Au)/indium tin oxide (ITO) film and gold nanoparticles (AuNPs), enabling simultaneous electrochemical and optical interrogation. The coupling effect between the SPR of thin film and the localized surface plasmon resonance (LSPR) of AuNPs significantly improves optical sensitivity, with AuNPs also offering additional active sites for the redox reaction of Hg2+. The ITO film not only facilitates the stripping of Hg2+, leading to sharper stripping peaks but also enhances the ability of the sensor to rapidly respond to anomalous potential changes. Experimental results show that the sensor has a wide dynamic detection range from 10−10 M to 10−5 M, with a limit of detection reaching the pM level. The dual-mode functionality allows the simultaneous collection of voltage, current, and optical information, enabling cross-validation of the detection results and improving the accuracy and reliability of detection.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zijian Li,Zijie Tang,Jilin Yan,Hao Peng,Yifeng Tu
Stroke, or cerebral apoplexy, is a paroxysmal disease that seriously threatens the neurological functions of middle-aged and older men, resulting in high rates of disability and mortality. The blood level of Corin can accurately predict the risk and subtype of stroke. Herein, an immunosensor was developed utilizing a nanocomposite of Au nanoparticles and Co 3 O 4 nanocubic cages (AuNPs/Co 3 O 4 NcC) as the sensing substrate, with luminol serving as the sensing probe for Corin detection. Through the application of 3-aminopropyltrimethoxysilane, AuNPs/Co 3 O 4 NcC was affixed to the surface of indium tin oxide (ITO)-coated glass, considerably enhancing the electrochemiluminescence (ECL) performance of luminol due to its peroxidase-like activity. To confer biological specificity by immobilizing the Corin antibody on this substrate and to block residual active sites with bovine serum albumin, an immunosensor was developed. The capture of Corin protein by the antibody through an immune reaction, leading to its binding on the sensor, resulted in a decreased ECL signal. Under optimized conditions, the proposed ECL immunosensor demonstrated a broad linear response range from 1 pg/mL to 4800 pg/mL and a low detection limit of 0.26 pg/mL. This ECL immunosensor is characterized by its specificity, stability, and reproducibility and can detect Corin in real blood samples.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）