ACS Agricultural Science & Technology
·
2023
·
IF 2.5
Liang Jin,Xiao-Di Zhao,Jun-Ao Liao,Li-Jun Fu,Yi Lin
Cry toxins produced by Bacillus thuringiensis (Bt) are toxic to Lepidoptera, Coleoptera, and Diptera, but display very low activity against aphids. Recently, Cry41-related toxin was found to show moderate toxicity against Myzus persicae with the 50% lethal concentration (LC50) of 32.7 μg/mL, and its mode of action was proposed to be the acceleration of the apoptosis of aphid cells by enhancing Cathepsin B activity. This study focused on constructing Cry41-related mutants against M. persicae based on its interaction with Cathepsin B. First, eight key interacting residues in Cry41-related toxin were identified using alanine scanning and site-directed saturation mutagenesis. Subsequently, the positive mutant Cry41-7M protein (mutations of Gly48, Ile59, Lys364, Gln367, Gln377, Tyr378, and Ser400 to Tyr, Ala, Arg, Lys, Lys, Lys, and Ala in Cry41-related toxin, respectively) and the negative mutant Cry41-6A protein (mutations of Gly48, Lys364, Gln367, Gln377, and Tyr378 to Ala and mutation of Pro453 to Glu in Cry41-related toxin) were constructed, expressed, and purified. We then found that Cry41-7M protein performed slightly stronger than Cry41-related toxin in enhancing the enzymatic activity of Cathepsin B, whereas Cry41-6A protein did not affect Cathepsin B activity. A further bioassay showed that the mortality caused by Cry41-7M protein (LC50 = 19.144 μg/mL) was marginally higher than that of Cry41-related toxin, while Cry41-6A protein caused a decreased mortality (LC50 = 42.478 μg/mL). These results are expected to open new avenues for improving Cry aphidicidal activity.
研究领域:
昆虫学
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分子生物学
·
生物技术
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DOI
高影响因子
ASTRONOMICAL JOURNAL
·
2017
·Vol. 153
·期 3
·138
·
IF 2.609
J. L. Birkby,R. J. de Kok,M. Brogi,H. Schwarz,I. A. G. Snellen
We report the detection of water absorption features in the day side spectrum of the first-known hot Jupiter, 51 Peg b, confirming the star–planet system to be a double-lined spectroscopic binary. We use high-resolution (R\approx 100,000), 3.2\,\mu {\rm{m}} spectra taken with CRIRES/VLT to trace the radial-velocity shift of the water features in the planet's day side atmosphere during 4 hr of its 4.23 day orbit after superior conjunction. We detect the signature of molecular absorption by water at a significance of 5.6\sigma at a systemic velocity of {V}_{\mathrm{sys}}=-33\pm 2 km s−1, coincident with the 51 Peg host star, with a corresponding orbital velocity {K}_{{\rm{P}}}={133}_{-3.5}^{+4.3} km s−1. This translates directly to a planet mass of {M}_{{\rm{p}}}={0.476}_{-0.031}^{+0.032}\,{M}_{{\rm{J}}}, placing it at the transition boundary between Jovian and Neptunian worlds. We determine upper and lower limits on the orbital inclination of the system of 70^\circ \lt i\lt 82\buildrel{\circ}\over{.} 2. We also provide an updated orbital solution for 51 Peg b, using an extensive set of 639 stellar radial velocities measured between 1994 and 2013, finding no significant evidence of an eccentric orbit. We find no evidence of significant absorption or emission from other major carbon-bearing molecules of the planet, including methane and carbon dioxide. The atmosphere is non-inverted in the temperature–pressure region probed by these observations. The deepest absorption lines reach an observed relative contrast of 0.9\times {10}^{-3} with respect to the host star continuum flux at an angular separation of 3 milliarcseconds. This work is consistent with a previous tentative report of K-band molecular absorption for 51 Peg b by Brogi et al.
研究领域:
天文学
·
行星科学
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DOI
高影响因子
CELLULAR SIGNALLING
·
2025
·
IF 3.7
Haowen Fang,Xiaodong Sun,Yanting Ding,Bing Niu,Qin Chen
Diabetic kidney disease (DKD) is a microvascular complication of diabetes with high morbidity and mortality, necessitating effective treatment. In this study, the Loureirin B analogue (LB-A) was utilized to treat DKD in mice. The results demonstrated that LB-A effectively prevent the progression of DKD in mice, significantly lowering fasting blood glucose levels and reducing proteinuria levels. Additionally, there was a significant decrease in oxidase content in the kidneys of mice, accompanied by an increase in antioxidant oxidase content, resulting in a decrease in ROS levels, mitigating oxidative stress state through modulation of Cxcl1. Cell experiments further confirmed that reducing Cxcl1/Cxcr2 axis activation prevented the onset of DKD induced by high glucose exposure and affected the therapeutic effect of LB-A as well. These findings provide evidences to support that LB-A may mitigate oxidative stress by modulating the Cxcl1 signaling pathway, thereby contributing to renal protection in the context of DKD treatment.
研究领域:
医学
·
药理学
·
分子生物学
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DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2024
·
IF 2.3
Liu Hang,Liu Sixu,Liang Yaozhen,Sun Yifei,Xiong Haifeng
Palladium (Pd)-based catalysts are widely used for CO 2 hydrogenation reactions due to the capability to activate H 2 , which enhances the overall CO 2 conversion. However, most of the current Pd catalysts reported show high CO 2 conversion only at higher temperature, and the exploration of efficient catalyst enabling low temperature conversion (180–300 °C) is of importance. In this study, a series of Pd-B/ZnAl 2 O 4 catalysts were synthesized via in-situ doping, resulting in the Pd crystal transformation induced from crystal to amorphous. The catalytic hydrogenation activity of the amorphous Pd-B catalyst reaches its zenith at a Pd-B mole ratio of 1:2, accompanied by the superior catalysis stability in CO 2 hydrogenation. Various characterization techniques, including XRD, CO-DRIFTS, H 2 -TPR, XPS and TEM, were used to investigate the properties of the Pd-B/ZnAl 2 O 4 catalyst. The results showed that the Pd-B/ZnAl 2 O 4 catalyst exhibited higher dispersion and better CO 2 conversion efficiency compared to unmodified Pd catalyst. Additionally, the methanol space–time yields are nearly doubled when the B decoration is employed. At 280 °C, CO 2 conversion was improved and methanol selectivity remained at ~ 65%. This study provides valuable insights into the design and optimization of Pd-B based catalysts for CO 2 hydrogenation at low temperatures. Graphical Abstract
研究领域:
催化化学
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能源化学
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材料科学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2012
·
IF 3.832
Yunjiao Wang,Peng Liu,Chao Yang,Bin Mu,Aiqin Wang
The rhodamine B-attapulgite/polypyrrole (RhB-ATP/PPy) composites have been prepared by the in situ chemical oxidative polymerization method based on the self-assembly of pyrrole on the surface of the rhodamine B modified ATP (RhB-ATP). The morphologies of the RhB-ATP/PPy composites prepared with different feeding ratio of pyrrole and the RhB-ATP were compared with transmission electron microscope (TEM) technique. The electrochemical behaviors of the RhB-ATP/PPy composites were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and galvanostatic charge–discharge. The results indicated that RhB did effectively improve the electrochemical performance and cycling stability of the RhB-ATP/PPy composite electrode, although RhB showed no obvious effect on the morphology of the nanocomposites . It was attributed to the fact that RhB and ATP provided a more convenient pathway to the charge carrier and RhB made the combination of the ATP and PPy much stronger. The maximum discharge capacity of the composites electrode achieved 587 F g −1 and kept 76% of its original specific capacitance after 500 cycles.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2015
·Vol. 44
·期 29
·13030-13039
·
IF 4.197
Hongmei Chen,Yinghao Xie,Xiaoqin Sun,Meilin Lv,Fangfang Wu,Lei Zhang,Li Li,Xiaoxiang Xu
Separation of photo-generated charges has played a crucial role in controlling the actual performance of a photocatalytic system. Here we have successfully fabricated g-C3N4/TiO2-B nanowire/tube heterostructures through facile urea degradation reactions. Owing to the effective separation of photo-generated charges associated with the type-II band alignment and intimate interfacial contacts between g-C3N4 and TiO2-B nanowires/tubes, such heterostructures demonstrate an improved photocatalytic activity over individual moieties. Synthetic conditions such as hydrothermal temperatures for the preparation of TiO2-B and the weight ratio of TiO2-B to urea were systematically investigated. A high crystallinity of TiO2-B as well as the proper growth of g-C3N4 on its surface are critical factors for a better performance. Our simple synthetic method and the prolonged lifetime of photo-generated charges signify the importance of type-II heterostructures in the photocatalytic applications.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 26
·15033-15041
·
IF 3.049
Ya Gao,Yongjie Zheng,Jixing Chai,Jingzhi Tian,Tao Jing,Deqing Zhang,Junye Cheng,Huiqing Peng,Bin Liu,Guangping Zheng
Effective separation and rapid transfer of photogenerated electron–hole pairs are key features of photocatalytic materials with high catalytic activity, which could be achieved in co-catalysts. It is reported that the two-dimensional (2D) MoS2 is a promising co-catalyst due to its unique semi-conductive properties and graphene-like layered structure. However, the application of MoS2 as a co-catalyst is limited by its poor electrical conductivity. On the other hand, it is worth noting that TiO2 possesses reactive crystal facets, which is one of the dominant mechanisms for the separation of photogenerated electron–hole pairs. In this work, we prepared MoS2/RGO hybrids as co-catalysts which were doped to TiO2 with highly reactive {001} planes via the hydrothermal method. It was found that the {001}-TiO2/MoS2/RGO photocatalysts with 7 wt% MoS2/RGO co-catalyst show the highest photodegradation activity for the degradation of Rh B under visible light irradiation (λ > 400 nm), which could result from the synergy of the effective separation of electron–hole pairs by the {001} facets in TiO2 and the rapid transfer of electron–hole pairs in MoS2/RGO. The results show that the {001}-TiO2/MoS2/RGO hybrid is a low-cost and stable photocatalyst for the effective degradation of Rh B under visible light.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xinyu Wang,Rui Li,Guoqing Zhao,Xiaorong Yan,Mingsheng Yang,Bei Wang,Haiping Yu,Mengxiao Li,Yuxin Hao,Zhihui Wang,Jingru Liu,Mingjun Hu,Jun Yang
Organic electrode materials are considered as a promising alternative to conventional transition metal-based electrodes because of their low cost, adjustable structure, high specific capacity, and good sustainability. However, many organic molecule electrodes and their corresponding discharge products can be easily dissolved in liquid electrolytes, leading to severe shuttle effect and performance decay. Here, we designed a separator with double carbon layer modification. The first layer is a B 4 O 7 2− cross-linked GO layer, which serves as a barrier to prevent organic small molecules from passing through the separator attributed to physical blocking and electrostatic repulsion. The second layer is a carbon nanotube layer, which mainly acts as an absorbing layer and upper current collector attributed to porous structure and good conductivity. The synergistic effect of double carbon layer enables the GO-B 4 O 7 2− @CNT-modified separator to effectively inhibit the shuttling of small molecules while maintaining their intrinsic electrochemical performance. Three soluble organic electrode materials with different molecular sizes, including 2,3,5,6-tetraamino-benzoquinone (TABQ), 9,10-anthraquinone (AQ), and 3,4,9,10-perylenetetracarboxylic diimide (PTCDI), were used as model molecules to validate the availability of modified separator, and the results shows that their cycling stability, specific capacity and rate capability were enhanced significantly after using the separator. Such simple cost-effective separator modification method will substantially promote the battery performance and facilitate the application of organic small molecule electrodes for energy storage.
研究领域:
材料科学
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电化学
·
储能
·
纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 15
·12447-12456
·
IF 2.779
Li Z. L.,Li S. Y.,Liu X. R.,Yuan S. Y.,Zhang Z. R.,Feng E. K.,Yang Z. M.,Han X. N.
In view of photodegradation of antibiotics based on SnS 2 , larger specific surface area SnS 2 microcubes remain rare. In this paper, shape-controlled SnS 2 microcubes with defects were successfully prepared via a simple template-engaged strategy. The obtained microcubes manifested high specific surface area (43.80 m 2 g −1 ). The degradation performance for Rhodamine B (RhB) was investigated in detail, including the effect of parameters, like different photocatalysts, pH, and catalyst dosage. The results indicated that degradation efficiency was 99.90% under visible light within 80 min. Moreover, the degradation efficiency was 79% after the four successive cycles.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2015
·
IF 2.515
Li-Ping Wang,Ke-Yang Yin,Guang Li,Qian Liu,Ai-Xia Deng,Hui-Yan Ma
Two types of star polystyrene (SPS) were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization: three-arm star polystyrene (TSPS) and five-arm star polystyrene (FSPS). Honeycomb-structured porous films were fabricated by drop-casting of the SPS solutions on glass substrates . The regular honeycomb-patterned porous films were successfully constructed from the SPS solution casting process , and the pore diameter increased with the molecular weight of the SPS enhanced at appropriate molecular weight range. Furthermore, rhodamine B (RhB) was loaded into SPS to endow the microporous film with fluorescence. The RhB-loaded SPS film exhibits a strong and uniform red emission image.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2025
·
IF 2.9
He Yong,Lin Wensong,Gao Ran,Zhang Yeheng,Lin Huanxia
Bi 5 O 7 I was considered as a promising photocatalyst due to its unique structure. However, the recombination rate of electron–hole pairs generated by Bi 5 O 7 I is too fast, making it impossible for practical applications. In this study, Bi 5 O 7 I catalysts deposited with various amount of silver were manufactured and their photocatalytic efficiency for rhodamine B (RhB) was measured. It was found that Bi 5 O 7 I with a silver deposition of 15% (named as Bi 5 O 7 I/Ag-15) exhibits the highest catalytic performance, with a degradation rate of 97.34% for RhB after illumination for 90 min. The cyclic test results confirmed that Bi 5 O 7 I/Ag-15 still maintained high photocatalytic activity after six cycles of degradation. In addition, Bi 5 O 7 I/Ag-15 also exhibits an excellent photocatalytic performance in acidic or alkaline environments, indicating its potential for practical applications. A reasonable degradation mechanism for RhB photocatalysis was then proposed. Graphical abstract
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Inorganics
·
2025
·Vol. 13
·期 3
·88
·
IF 3
Jingjing Dai,Guofei Li,Yuanyuan Wang,Cancan Zhang,Hui Nan,Guijun Yang
During the process of producing potassium fertilizer from salt lake resources, a large amount of waste liquid brine, rich in raw materials such as magnesium chloride, is generated. In this work, a MoS2/MgAl-LDH composite material was constructed using the secondary hydrothermal technique. Characterizations including X-ray diffractometer (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) confirmed the distribution of MoS2nanosheets on the surface of MgAl-LDH. Under full-spectrum irradiation, the degradation efficiency of Rhodamine B reached 85.5%, which was 69.2% higher than that of MgAl-LDH alone. The results from the electrochemical, UV-Vis, and XPS-VB tests indicate that the internal electric field accelerated the separation and transportation of charge carriers between MoS2and MgAl-LDH. These findings demonstrate the great potential of MoS2/MgAl-LDH as a photocatalyst in the degradation of organic dyes, which will aid in the green recycling utilization of magnesium resources from salt lake by-products.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
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