JOURNAL OF SEPARATION SCIENCE
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2013
·Vol. 36
·期 8
·1343-1348
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IF 2.591
Qiuyun Wang,Yajin Xiong,Baozhu Lu,Jun Fan,Sheng Zhang,Shengrun Zheng,Weiguang Zhang
N -(2,4-dinitrophenyl)-proline and N -(2,4-dinitrophenyl)-serine were enantiomerically resolved on the BSA chiral stationary phase by HPLC in reversed-phase mode. Effects of chromatographic conditions on enantioseparation and elution order have been investigated in detail. For these two samples, reversal of enantiomer elution order was observed by changing buffer pH, the content of acetonitrile, or alcohol modifiers in mobile phase, which is firstly reported in the BSA chiral stationary phase studies. More interestingly, combined effect between buffer pH and the content of acetonitrile was also observed. In addition, coelution range of enantiomers varied along with the content of acetonitrile in mobile phase.
研究领域:
分析化学
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生物化学
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PubMed
高影响因子
Nanoscale Advances
·
2025
·
IF 4.6
Shuaibo Yang,Yanzhao Diao,Lifeng Hang,Hong Qu,Laiping Fang,Wei Guo,Hua Wen,KuokWai IU,Guihua Jiang,Lianyi Shao,Quan Li
Multifunctional phototherapy that integrates diagnostic and therapeutic modalities holds the potential to revolutionize cancer treatment. The near-infrared cyanine dye IR780 is known for its high spatial resolution imaging capabilities. When conjugated with bovine serum albumin (BSA), it effectively mitigates common challenges such as photobleaching and fluorescence quenching, making it widely used in tumor imaging. However, its limited photothermal conversion efficiency hinders its broader application in tumor therapy. To overcome these limitations, this study presents the design and development of an imaging-guided multifunctional nanoplatform: mesoporous polydopamine (MPDA)-BSA@IR780. Our findings demonstrate that MPDA, as a carrier, significantly improves the photostability of BSA@IR780. Furthermore, MPDA's superior photothermal properties enhance the photothermal efficacy of the platform, enabling MPDA-BSA@IR780 to function as a dual photothermal therapy (PTT) agent. In addition to its therapeutic potential, MPDA-BSA@IR780 serves as both a photoacoustic (PA) and fluorescence (FL) imaging probe, effectively guiding treatment decisions. Cellular assays reveal that MPDA-BSA@IR780 exhibits a robust photothermal effect, supporting promising therapeutic outcomes. In vivo studies further demonstrate that, following laser irradiation, MPDA-BSA@IR780 achieves near-complete tumor ablation without inducing significant toxicity, while also exhibiting excellent biocompatibility. In conclusion, this study introduces a safe and effective photothermal nanoparticle platform for tumor imaging, diagnosis, and treatment, providing a promising strategy for future biomedical applications.
研究领域:
癌症研究
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纳米技术
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诊疗学
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生物医学工程
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PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Jingyao Mao,Yuteng Zhang,Shensong Zhang,Bo Song
The fluorescent probe method has attracted significant research attention due to its high sensitivity and reproducibility in detecting bovine serum albumin (BSA). In this study, we constructed a fluorescent probe for BSA detection by assembling an amphiphilic organic fluorescent molecule, termed 2-(2’-hydroxyphenyl) benzothiazole (HBT-11), with BSA. In an aqueous solution, HBT-11 exhibited a weak fluorescence emission at 501 nm. However, the addition of BSA substantially enhanced the fluorescence emission at 501 nm, indicating that the assembly was driven by electrostatic interactions between HBT-11 and BSA. HBT-11, serving as a fluorescent probe for BSA detection, demonstrated a limit of detection (LOD) as low as 3.92 nmol L–1, excellent photostability, high selectivity, and robust anti-interference capability. Notably, we successfully applied HBT-11 for detecting BSA in fetal bovine serum and selectively imaging BSA in HeLa cells.
研究领域:
分析化学
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生物化学
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分子生物学
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细胞生物学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Lian Xing,Jinchen Sun,Huaping Tan,Guoliang Yuan,Jianliang Li,Yang Jia,Dangsheng Xiong,Guang Chen,Jianzhong Lai,Zhonghua Ling,Yong Chen,Xiaohong Niu
Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery. This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering. This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents. The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N -succinyl chitosan ( N -Chi) and oxidized alginate (OAlg), respectively. Meanwhile, gel-like microspheres were prepared with a diameter of 2–10 μm by conjugating sodium alginate with Ca 2+ in an aqueous emulsion via the emulsion cross-linking technique. Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/ N -Chi hydrogels to produce a composite scaffold. In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined. The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres. The alginate microspheres can serve as a filler to toughen the soft OAlg/ N -Chi hydrogels. Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres. Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone. These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
研究领域:
生物材料
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组织工程
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药物递送系统
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Qihao Zhu,Weiran Mao,Chengli Zhang,Yuan Zhou,Zhiyong Tang,Chao Yu
Here, a novel electrochemiluminescence biosensor based on potential-resolved strategy was firstly prepared for the detection of dual targets α2,3-sialylated glycans and α2,6-sialylated glycans. This is the first time that Au@BSA microsphere was used to connect with luminol to enhance its ECL intensity, and it can generate ECL signals at positive potential. Zeolitic Imidazolate Framework-8 (ZIF-8) and Meso-tetra (4-carboxyphenyl) porphyrin (TCPP) were linked using a one-pot method to synthesize a novel luminescent ZIF (L-ZIF) named TZZ, which can emit ECL signals at negative potential. Moreover, magnetite microspheres were used to construct a sandwich-type biosensor to obtain higher sensitivity and reduce background signals. In addition, the biosensor manufactured directly in solution have a wider linear range than constructed on electrode because it has more available space than the electrode surface. Due to the above advantages, the prepared ECL biosensor exhibited high sensitivity, stability and broader linear range, even for practical analysis. Therefore, the prepared ECL biosensor will become a promising method for determination of α2,3-sialylated glycans and α2,6-sialylated glycans in clinical applications in the future. What is more, it provides a potential method for detection of other multi-targets.
研究领域:
分析化学
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生物医学工程
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传感技术
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yu Xiao,Na Huang,Jiahui Wen,Dan Yang,Huanhuan Chen,Yijuan Long,Huzhi Zheng
Fluorescent bovine serum albumin-protected gold nanoclusters ( [email protected] NCs) can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxTMB for its peroxidase-like activity. The two absorption peaks of oxTMB overlapped with the excitation and emission peaks of [email protected] NCs, respectively, causing efficient quenching on the fluorescence of [email protected] NCs. The quenching mechanism can be attributed to the dual inner filter effect (IFE). Based on the dual IFE, [email protected] NCs were utilized as both peroxidase mimics and fluorescent reporters for H 2 O 2 detection and further for uric acid detection with uricase. Under optimal detection conditions, the method can be used to detect H 2 O 2 ranging 0.50–50 μM with a detection limit of 0.44 μM and UA ranging 0.50–50 μM with a detection limit of 0.39 μM. The established method had been successfully utilized for the determination of UA in human urine, with massive potential in biomedical applications.
研究领域:
分析化学
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纳米技术
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生物医学工程
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 8
·1477-1485
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IF 4.3
Yuan Zhenfeng,Li Zhe,Zhao Xiaoxue,Ding Zhenyu,Xia Mingyuan,Dong Xuyang,Ni Pengjuan,Lu Yizhong
Hexavalent chromium is a highly toxic substance, which will pose a serious threat to human life and health and the entire ecosystem. Therefore, it is crucial to establish a simple and rapid detection method for hexavalent chromium. In this work, we fabricated bovine serum albumin–stabilized silver nanocluster (BSA-Ag 13 NC) which exhibited photoresponsive oxidase-like activity, catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue oxidized state TMB (oxTMB) in a short time. Interestingly, 8-hydroxyquinoline (8-HQ) can significantly inhibit the color reaction of TMB oxidation while Cr(VI) can interact specifically with 8-HQ to restore this chromogenic reaction. Based on the above facts, a colorimetric sensing system for detecting Cr(VI) was developed. The sensing system shows a wide linear range, and good selectivity, with a low detection limit of 2.32 nM. Moreover, this sensing system could be successfully applied to the detection of Cr(VI) in lake water, tap water, and sewage with satisfactory results.
研究领域:
纳米技术
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环境科学
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分析化学
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PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Han Hu,Ruiqi Li,Piao Huang,Zhimin Mo,Qi Xu,Tao Hu,Shijie Yao,Xiaofang Dai,Zushun Xu
Photodynamic therapy (PDT) has many exceptional advantages in cancer treatment, such as minor trauma, low toxicity side effects, and strong adaptability, effectively overcoming some obstacles of traditional therapy and providing more revolutionary opportunities for curing cancer. Chlorin e6 (Ce6) exhibits excellent singlet oxygen generation and conversion efficiency under near-infrared laser irradiation and is a promising PDT photosensitizer. However, its hydrophobicity, short half-life and lack of tumor specificity limit its in vivo anticancer application. Therefore, this work has designed and prepared a multifunctional nanoplatform, Ce6/ [email protected] , to efficiently deliver Ce6. Nanoparticles exhibit excellent dispersion and stability in deionized water, PBS and DMEM, and the blood half-life is 3.98 ± 0.31 h. The nanoplatform demonstrates effective tumor targeting and accumulation, overcoming the obstacles of the biological application of Ce6. Iron ions can exert a chemodynamic therapy (CDT) effect by reacting with overexpressed H 2 O 2 in the tumor to generate toxic hydroxyl radicals (·OH). Moreover, FeOOH nanoparticles effectively promote glutathione (GSH) consumption in tumor cells, which is conducive to accumulating reactive oxygen species (ROS). In brief, Ce6/ [email protected] nanoparticles realize the targeted delivery of Ce6 and mediate synergistic PDT/CDT against tumors, broadening the biomedical application of nanomaterials.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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药学
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PubMed
高影响因子
Journal of Cleaner Production
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2021
·
IF 9.297
Wenning Yang,Jia Li,Yangsai Lyu,Xiaohui Yan,Ping Yang,Min Zuo
Artificial self-propelled micro/nano-motors have emerged as a promising hot spot in the fields of water-body remediation and environmental protection. Herein, we report a novel multifunctional micromotors with dual enzyme-like activities for simultaneous colorimetric determination and removal of environmental contaminants. Thanks to the tubular morphology inherited from kapok fibers, these bovine serum albumin-functionalized NiCo 2 O 4 @MnO 2 /C (BSA-NiCo 2 O 4 @MnO 2 /C) micromotors with dual oxidase/peroxidase-like activity can move forward quickly in aqueous solutions via a bubble recoil mechanism, which can not only enhance the colorimetric sensing performance, but also improve the removal efficiency toward Cu 2+ ion by enhancing the mass transfer both in the detection and catalytic degradation systems. Moreover, the unique 3D hierarchical architecture assembled by core-shell NiCo 2 O 4 @MnO 2 nanosheets and C microtubes can provide more accessible reactive sites and thus dramatically facilitate such detection process. In combination with the autonomous motility, intrinsic oxidase/peroxidase-like activities and precisely tuned hierarchical architecture, these automatic micromachines not only are able to detect Cu 2+ selectively and sensitively with the limit of detection of 2.0 nM, but also quickly remove Cu 2+ from wastewater with maximal adsorption capacity of 161.8 mg g −1 . Furthermore, these micromotors exhibit excellent catalytic activity for the degradation of tetracycline hydrochloride. This multifunctional mobile platform will provide a new and promising strategy for the simultaneous detection and decontamination of poisonous metal ions and other pollutants in aqueous samples.
研究领域:
环境科学
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纳米技术
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分析化学
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水处理
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高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2019
·
IF 3.31
Yun Li,Yueqin Li,Nana Wang,Dong Lin,Xiaohui Liu,Yong Yang,Qinwei Gao
Two new nickel (II) triphenylphosphine complexes derived from tridentate aroylhydrazone ligands [H2L1 = 2-hydroxy-3-methoxybenzylidene)benzohydrazone and H2L2 = N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazone] and triphenylphosphine were prepared and their molecular structures were determined by single crystal X-ray diffraction analysis. Both nickel(II) complexes showed slightly distorted square planar geometry with one tridentate aroylhydrazone ligand coordinated through ONO donor atoms and one triphenylphosphine ligand coordinated to the nickel center through the phosphorus atom. DNA interaction studies indicated that both complexes possessed higher affinity to herring sperm DNA (HS-DNA) than the corresponding free aroylhydrazone ligand. Molecular docking investigations showed that both complexes could bind to DNA through intercalation of the phenyl rings between adjacent base pairs in the double helix. Meanwhile, bovine serum albumin (BSA) binding studies revealed the complexes could effectively interact with BSA and change the secondary structure of BSA. Further pharmacological evaluations of the synthesized complexes by in vitro antioxidant assays demonstrated high antioxidant activity against NO· and O2˙− radicals. The anticancer activity of each complex was assessed through in vitro cytotoxicity assays (CCK-8 kit) toward A549 and MCF-7 cancer cell and normal L-02 cell lines. Significantly, the Ni(II) complex derived from H2L1 ligand was found to be more effective cytotoxic toward MCF-7cancerous cell with the IC50 value equaled 9.7 μM, which showed potent cytotoxic activity over standard drug cisplatin. AbbreviationsA549human lung carcinoma cellBSAbovine serum albuminCCK-8Cell Counting Kit-8DFTdensity functional theoryDNAdeoxyribonucleic acidDPPH˙2,2-diphenyl-1-picrylhydrazylH2L12-hydroxy-3-methoxybenzylidene)benzohydrazone N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazoneH2L2N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazoneHOMOhighest occupied molecular orbitalIC50the 50% activityL-02human normal liver cellLOMOlowest unoccupied molecular orbital (LUMO)MCF-7human breast carcinoma cellNO˙nitric oxideO2˙−superoxide anionSODsuperoxide dismutaseCommunicated by Ramaswamy H. Sarma
研究领域:
无机化学
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生物化学
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药理学
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癌症研究
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DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Jing Lan,Hong Yan Zou,Qiang Wang,Ping Zeng,Yuan Fang Li,Cheng Zhi Huang
An ultra-sensitive and selective turn off-on fluorescence detection of heparin based on the energy transfer in the BSA-stabilized gold nanoclusters/amino-functionalized graphene oxide (BSA-AuNCs/NH 2 -GO) hybrids was successfully realized. The BSA-AuNCs containing amounts of carboxyl groups could be absorbed on the surface of NH 2 -GO through the electrostatic interaction , which resulted in the fluorescence quenching of BSA-AuNCs with high efficiency. However, heparin, possessing high density of negative charge, could compete with BSA-AuNCs to bind NH 2 -GO and block the energy transfer from BSA-AuNCs to NH 2 -GO. The fluorescence recovery of BSA-AuNCs was closely related to the amount of heparin and there was a good linear relationship between fluorescence recovery of BSA-AuNCs and heparin over the range of 100 ng/mL to 30 μg/mL with a detection limit of 40 ng/mL. What's more, the fluorescence assay was successfully applied for heparin sensing in human serums and intracellular imaging.
研究领域:
生物化学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Hong Zhuang,Xiaoliang Zhang,Sijia Wu,Chen Mao,Yaxi Dai,Pang Yong,Xiaodi Niu
Hesperidin (HE), a significant flavonoid polyphenolic compound present in citrus plants, exhibits diverse pharmacological effects. Considering the crucial involvement of biological membranes and transporter proteins in the transportation and biological processes of HE, it becomes essential to comprehend the potential mechanisms through which HE interacts with membranes and transporter proteins. In order to simulate the process of active molecule transport, a cell membrane model consisting of 1,2-dipalmitoyl-n- glycero -3-phosphatidylcholine (DPPC) and a transporter protein model of bovine serum albumin (BSA) were employed for investigation. The present study aimed to investigate the mechanism of action of hesperidin (HE) in DPPC and BSA using fluorescence quenching, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). The localization and interaction of HE within liposomes were also elucidated. Furthermore, the binding of BSA and HE was analyzed through UV/Vis absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and computational biology techniques. Computational biology analysis revealed that the binding between HE and BSA primarily occurred via hydrogen bonding and hydrophobic interactions. This study aimed to investigate the role and mechanism of HE in the DPPC cell membrane model and the BSA transporter protein model, thereby offering novel insights into the action of HE in DPPC and BSA.
研究领域:
药理学
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生物物理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）