BIOMEDICAL CHROMATOGRAPHY
·
2018
·Vol. 32
·期 8
·e4256
·
IF 1.688
Fugeng Zhang,Xiaoli Qiao,Hui Lu,Shaoqiang Zhang,Wuxun Du,Xuefeng Xiao
Yiqifumai Injection is a lyophilized powder preparation widely used to treat coronary heart disease. However, its in vivo bioactive components and pharmacokinetic behavior remain unknown. Therefore a sensitive and specific LC–MS/MS was developed and validated for the simultaneous quantification of eight saponins and four lignans in beagle dog plasma. The plasma samples were pretreated by protein precipitation with methanol–acetonitrile (1:1, v /v). Chromatographic separation of all the 12 analytes and estazolam (internal standard, IS) was successfully accomplished on an Ultimate® XB-C 8 column (100 × 2.1 mm, 3 μm) with a gradient elution system. The total running time was 8 min with a flow rate of 0.40 mL/min. Acquisition of mass spectrometric data was performed via positive electrospray ionization in multiple reaction monitoring mode. The assay was fully validated in terms of selectivity, linear range, lower limit of quantitation, precision, accuracy, matrix effect, recovery and stability. This validated method was successfully applied to the pharmacokinetics of 12 bioactive components after intravenous administration of Yiqifumai Injection to beagle dogs at a dose of 0.541 g/kg.
研究领域:
药理学
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分析化学
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兽医学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Yunchuan He,Yang Gao,Qiulin Chen,Hainuo Hong,Jiamei Geng,Ying Zhou,Zeng-Rong Zhu
Analyzing the role of herbivore-induced volatiles in plant–herbivore–natural enemy interactions is crucial for developing integrated pest management strategies. This study identified 1-octen-3-ol and 2-ethyl-1-hexanol as the primary C8 volatiles induced by Megalurothrips usitatus infestation in cowpea flowers. The concurrent accumulation of glycosides of these volatiles, along with the upregulation of LOX and HPL expression, suggested that the increased emissions of 1-octen-3-ol and 2-ethyl-1-hexanol result from enhanced de novo biosynthesis regulated by herbivore-induced jasmonate. 1-Octen-3-ol exhibited strong repellent effects against M. usitatus and attracted its natural enemy, Orius similis, at low concentrations. By contrast, 2-ethyl-1-hexanol repelled M. usitatus but did not exert a significant attractant effect on O. similis. These volatiles prime the defense mechanism in cowpea plants against M. usitatus, which highlight their promising role in controlling thirp populations and enhancing cowpea cultivation.
研究领域:
植物学
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生态学
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害虫管理
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Peng Fei,Ai Xiangnan,Bu Xiaoyu,Zhao Zixuan,Gao Baoxiang
Mitochondrial membrane potential (MMP) is crucial for mitochondrial function and serves as a key indicator of cellular health and metabolic activity. Traditional lipophilic cationic fluorescence intensity probes are unavoidably influenced by probe concentration, laser intensity, and photobleaching, limiting their accuracy. To address these issues, we designed and synthesized a pair of fluorescence molecules, OR-C8 and SiR-BA, based on the Förster Resonance Energy Transfer (FRET) mechanism, for dual-modality visualization of MMP. OR-C8 anchors to the inner mitochondrial membrane through strong hydrophobic interactions, while SiR-BA is expelled from mitochondria when MMP decreases, thereby regulating the FRET process. During MMP reduction, the fluorescence intensity and lifetime of OR-C8 increase, while the fluorescence intensity of SiR-BA decreases. By combining changes in fluorescence intensity ratio and fluorescence lifetime, dual-modality visualization of MMP was achieved. This method not only accurately reflects MMP changes but also provides a novel tool for in-depth studies of mitochondrial function and related disease mechanisms, offering significant potential for advancing mitochondrial research and therapeutic development.
研究领域:
细胞生物学
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生物物理学
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荧光显微镜技术
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2022
·Vol. 133
·期 5
·3161-3175
·
IF 4.059
Pan Deng,Wang Xing‐Yun,Zhou Jin‐Wei,Yang Liang,Khan Abbas,Wei Dong‐Qing,Li Jun‐Jian,Jia Ai‐Qun
AimsIn the current study the anti‐virulence and anti‐biofilm activities of the cinnamic acid derivative, 3‐methoxycinnamic acid, was investigated against Agrobacterium tumefaciens.Methods and ResultsBased on the disc diffusion test and β‐galactosidase activity assay, 3‐methoxycinnamic acid was shown to interfere with the quorum sensing (QS) system of A. tumefaciens. Crystal violet staining assay, phenol‐sulfuric acid method, Bradford protein assay and confocal laser scanning microscopy (CLSM) revealed that the biofilm formation of A. tumefaciens was inhibited after the treatment of 3‐methoxycinnamic acid. Employing high‐performance liquid chromatography (HPLC) analysis of culture supernatant revealed that the production of 3‐oxo‐octanoylhomoserine lactone (3‐oxo‐C8‐HSL) decreased concentration‐dependently after treatment with 3‐methoxycinnamic acid. Swimming and chemotaxis assays also indicated that 3‐methoxycinnamic acid had a good effect on reducing the motility and chemotaxis of A. tumefaciens. In addition, the RT‐qPCR, molecular docking and simulations further demonstrated that 3‐methoxycinnamic acid could competitively inhibit the binding of 3‐oxo‐C8‐HSL to TraR and down‐regulate virulence‐related genes.Conclusions3‐Methoxycinnamic acid is proved to have good anti‐virulence and anti‐biofilm activities against A. tumefaciens.Significance and Impact of the StudyThis is the first study that investigates the anti‐virulence and anti‐biofilm activities of 3‐methoxycinnamic acid against A. tumefaciens. With its potential QS‐related virulence and biofilm inhibitory activities, 3‐methoxycinnamic acid is expected to be developed as a potent pesticide or adjuvant for the prevention and treatment of crown gall caused by A. tumefaciens.
研究领域:
微生物学
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植物病理学
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药理学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2021
·Vol. 11
·期 37
·23045-23054
·
IF 3.361
Xinyan Sun,Xiang Zhang,Tao Zheng,Haiyan Liu,Rui Zhang,Xianghai Meng,Chunming Xu,Zhichang Liu
CuHY samples prepared by solid-state ion exchange of HY zeolite with CuCl were used as catalysts in isobutane/2-butene alkylation. The results show that both the addition amount of CuCl and the calcination temperature affect the ion exchange degree. Cu+ can be introduced into Y zeolite by replacing H+ in the HY zeolite after the solid-state ion exchange, causing a decrease of the amount of Brønsted acid sites and an increase of that of Lewis acid sites. When taking CuHY as the catalyst in isobutane/2-butene alkylation, the 3d104s0 valence electron configuration of Cu+ makes it favorable for inhibiting the oligomerization of 2-butene and accelerating the hydride transfer reaction rate by indirectly increasing the local isobutane/olefin ratio around the acid sites of the catalyst. As a result, the selectivity of C8 and trimethylpentanes over CuHY in alkylate are improved compared with those of HY.
研究领域:
化学工程
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催化
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石油化学
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2025
·
IF 8.4
Shumin Qin,Lin Deng,Yan Lin,Lang Jiang,Xilong Liu,Qian Zhang
To enhance the stability of membrane aerated biofilm reactor (MABR) and accelerate the formation of biofilm, this study introduced a symbiotic system composed of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria and Chlorella into the MABR, and compared it with a control system inoculated with HN-AD bacteria and activated sludge. The effects of adding 0.8 μM exogenous C8-HSL on biofilm formation and the efficiency of nitrogen and phosphorus removal were investigated by comparing systems with and without the signaling molecule. Results showed that 0.8 μM C8-HSL enhanced the removal efficiencies of ammonia nitrogen, total nitrogen (TN), and total phosphorus (TP) by 25.17 %, 36.22 %, and 38.74 %, respectively, and increased extracellular polymeric substances (EPS) production by 68.62 %. High-throughput sequencing identified Desulfomicrobium and Aliidiomarina as the dominant microorganisms involved in nitrogen and phosphorus removal. The nirB gene played a key role in the denitrification process, and phoBR gene was essential for phosphorus removal. This study provides theoretical support for the application of quorum sensing in MABR systems, offering a novel strategy to improve the efficiency and stability of high ammonia wastewater treatment.
研究领域:
环境工程
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微生物学
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生物技术
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废水处理
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Lingjie Liu,Shaoqing Mo,Shaopo Wang,Jing Chang,Jianhui Zhao,Jingjie Yu
Low temperatures were a critical factor that inhibited anaerobic ammonium oxidizing bacteria (AnAOB) activity. This study demonstrated the effect of exogenous zero-valent iron (ZVI) on Anammox-hydroxyapatite (HAP) system at 17°C. The results showed that long-term addition of ZVI increased the system nitrogen removal efficiency by 8.59% and alleviated the inhibition of AnAOB by low temperature. After addition of ZVI, the activity of AnAOB and the heme c content increased by 58.20% and 4.83%, respectively. Moreover, the functional enzyme activities of NIR, HZS, and HZO increased by 94.12%, 36.36%, and 16.67%, respectively. The concentrations of quorum sensing molecules, i.e., C 6 -HSL and C 8 -HSL, increased by 191.17% and 41.54%, respectively, after ZVI addition. Metagenomic analysis revealed that the addition of 5 g/L ZVI stimulated the relative abundance relative abundance of Fe(II) metabolism-related genes in AnAOB at 17 °C, thereby contributing to the uptake, transport, utilization, and storage of Fe(II). The addition of ZVI could improve the nitrogen removal performance of the anammox-HAP system at the low temperature.
研究领域:
环境工程
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微生物学
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生物技术
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2019
·Vol. 31
·期 16
·1900763
·
IF 25.809
Yuanhong Gao,Ya Yi,Xinwei Wang,Hong Meng,Dangyuan Lei,Xue-Feng Yu,Paul K. Chu,Jia Li
The interfacial charge effect is crucial for high-sensitivity organic phototransistors (OPTs), but conventional layered and hybrid OPTs have a trade-off in balancing the separation, transport, and recombination of photogenerated charges, consequently impacting the device performance. Herein, a novel hybrid-layered phototransistor (HL-OPT) is reported with significantly improved photodetection performance, which takes advantages of both the charge-trapping effect (CTE) and efficient carrier transport. The HL-OPT consisting of 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene (C8-BTBT) as conduction channel, C8-BTBT:[6,6]-phenyl-C 61 -butyric acid methyl ester (PC 61 BM) bulk heterojunction as photoactive layer, and sandwiched MoO 3 interlayer as a charge-transport interlayer exhibits outstanding photodetection characteristics such as a photosensitivity ( I light / I dark ) of 2.9 × 10 6 , photoresponsivity ( R ) of 8.6 × 10 3 A W −1 , detectivity ( D* ) of 3.4 × 10 14 Jones, and external quantum efficiency of 3 × 10 6 % under weak light illumination of 32 µW cm −2 . The mechanism and strategy described here provide new insights into the design and optimization of high-performance OPTs spanning the ultraviolet and near infrared (NIR) range as well as fundamental issues pertaining to the electronic and photonic properties of the devices.
研究领域:
光电子学
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有机电子学
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光电探测器
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材料科学
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纳米技术
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 8
·1872
·
IF 4.2
Daihua Hu,Yulin Wang,Fanshu Kong,Danni Wang,Chingyuan Hu,Xu Yang,Xiaohua Chen,Wang Chen,Zili Feng
Further assessment of ultraviolet C light-emitting diode (UVC-LED) irradiation for influencing shiitake mushrooms’ (Lentinus edodes) volatile and sensory properties is needed. In this study, a comparison of UVC-LED irradiation treatment on the flavor profiles in various parts of shiitake mushrooms was conducted using gas chromatography–ion mobility spectrometry (GC-IMS) and sensory analysis. Sixty-three volatile compounds were identified in shiitake mushrooms. The fresh shiitake mushrooms were characterized by the highest values of raw mushroom odors. After UVC-LED treatment, the content of C8 alcohols decreased, especially that of 1-octen-3-ol, while the content of aldehydes increased, especially the content of nonanal and decanal. The score of fatty and green odors was enhanced. For fresh samples, the mushroom odors decreased and the mushroom-like odors weakened more sharply when treated in ethanol suspension than when treated with direct irradiation. The fruit odors were enhanced using direct UVC-LED irradiation for fresh mushroom samples and the onion flavor decreased. As for shiitake mushroom powder in ethanol suspension treated with UVC-LED, the sweaty and almond odor scores decreased and the vitamin D2content in mushroom caps and stems reached 668.79 μg/g (dw) and 399.45 μg/g (dw), respectively. The results obtained from this study demonstrate that UVC-LED treatment produced rich-flavored, quality mushroom products.
研究领域:
食品科学
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农业工程
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分析化学
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感官评价
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Yali Li,Yi Zhang,Yibin Zhou,Yufei Zhang,Mingming Zheng
A novel and effective approach based on the two-step ethanolysis-esterification strategy was proposed for the controllable and simultaneous preparation of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and 1,3-dilinoleoyl-2-palmitoyl-glycerol (LPL) with adjustable proportions. Enzymatic ethanolysis of fractionated palm stearin was carried out to yield 2-monopalmitoylglycerol (79.4 ± 0.6 %) with over 91.0 % purity at the optimal conditions. The immobilized Candida sp. lipase (CSL) on octyl-functionalized ordered mesoporous silica (OMS-C 8 ) was applied to re-esterify 2-monopalmitoylglycerol with oleic acid and linoleic acid for the simultaneous production of OPL, OPO, and LPL. The total content in the final products was 81.5 %, with 91.3 % of palmitic acid (PA) content at the sn -2 position. Besides, OPL/OPO/LPL was conveniently prepared with suitable proportions for worldwide infants by adjusting the ratio of acyl donors. This paper provides a novel and effective two-step ethanolysis-esterification strategy for the development of human milk fat substitutes (HMFS).
研究领域:
食品科学
·
化学工程
·
生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）