SENSORS AND ACTUATORS B-CHEMICAL
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2026
·
IF 8.3
Zhenghao Li,Junjie Cui,Zhenyu Xu,Sha Xiong
Rapid and low-cost detection of bacteria in water is critical for ensuring drinking water safety and enabling effective public health surveillance. We introduce an optofluidic platform for quantifying bacteria by using catalase-mediated hydrogen peroxide consumption, eliminating the need for exogenous bio-recognition probes. Sample handling and optical readout are integrated in the optofluidic chip through a sequential process: the water sample is first mixed with hydrogen peroxide to trigger catalase-catalyzed reactions, then combined with a chromogenic substrate for detection. High-sensitivity absorbance measurement for the Fe 3 + –sulfosalicylic acid complex is enabled by an integrated optical waveguide. Using Escherichia coli ( E. coli ) as a model strain, the method exhibits a linear dynamic range of 5 × 10 1 –5 × 10 6 CFU/mL and a limit of detection of 4 CFU/mL. The platform was further validated using Staphylococcus aureus ( S. aureus ) and Bacillus subtilis ( B. subtilis ), confirming its broad applicability across catalase-positive bacterial species. Live/dead bacterial discrimination experiments demonstrated that the detection signal originates exclusively from metabolically active viable cells. Interference tests and analysis of real water samples verified the selectivity and accuracy of the method. Detection results are available within 10 min, enabling continuous operation with high sensitivity. This optofluidic approach provides a novel on-site technique for rapid bacterial detection and holds promising application prospects in online industrial and environmental monitoring.
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JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
·
2026
·
IF 3.5
Haoran He,Wanli Ma,Zhiqiang Fan,Kai Xu,Zhiqiang Kang,Wei Zhao,Le Zhang,Xiaojun Qiao,Wenping Geng
With the advancement of micro-nano manufacturing technologies, microheaters have demonstrated significant application value in fields such as sensing, ignition, and self-destruction systems. This paper designs and fabricates a parallel necked Ni microbridge microheater based on microelectromechanical systems (MEMS), employing a Ti/Cu/Ni metal multilayer structure on a SiO2/Si/SiO2 substrate to achieve a compact, integrable device configuration. Finite element simulations revealed the temperature distribution and evolution patterns under different current conditions, which were subsequently validated experimentally. Test results demonstrated that the device rapidly heats up under constant current drive, reaching a maximum temperature exceeding 600°C at 1.8 A drive conditions, exhibiting excellent electrothermal response characteristics. Further integrated validation demonstrates that the proposed microheater structure can achieve stable and controllable triggering under system-level packaging conditions. Under a driving current of 3 A, the device completes the self-destruction process within 1 s, accompanied by pronounced current drop and voltage surge characteristics, indicating that the designed electrothermal structure can satisfy the ignition requirements of porous silicon energetic chips at the system level, thereby reflecting favorable structural compatibility and design rationality. [2025-0234]
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Analytical Methods
·
2015
·Vol. 7
·期 6
·2406-2412
·
IF 1.821
Weiwen Hu,Qiong Hu,Lianzhi Li,Jinming Kong,Xueji Zhang
In this work, an efficient method for the sequence-specific detection of DNA based on a morpholino-functionalized silicon chip platform has been proposed. Briefly, morpholino was first immobilized on the surface of a silicon chip using 3-aminopropyltriethoxysilane (APTES) as the silane coupling agent and 1,4-phenylenediisothiocyanate (PDITC) as the cross-linker and then hybridized with DNA in the ensuing step. The fluorescence label was introduced by strongly binding Rhodamine B, which contains a terminal carboxylic group, with DNA by means of phosphate–zirconium–carboxylate coordination reaction. X-ray photoelectron spectroscopy (XPS) was used to characterize the silicon surface. Under optimal conditions, the morpholino-functionalized silicon chip presented a great linear relationship between the fluorescence intensity and the logarithm of target DNA concentrations in the range from 1 pM to 1 nM with a detection limit of 4.52 pM. Furthermore, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively identified. The chip showed excellent stability because it could be reused for another hybridization experiment after denaturing the morpholino–complementary DNA duplex. In addition, the chip rendered satisfactory analytical performance for the detection of DNA in serum samples, thus exhibiting practical significance. Morpholino-functionalized silicon chips display good sensitivity and selectivity for the detection of DNA and promising applications in single-nucleotide polymorphisms (SNPs).
研究领域:
生物化学
·
分子生物学
·
分析化学
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ACS Nano
·
2022
·
IF 18.027
Xia Yin,Jing Yang,Mengji Zhang,Xinyao Wang,Wei Xu,Cameron-Alexander H. Price,Lin Huang,Wanshan Liu,Haiyang Su,Wenjing Wang,Hongyu Chen,Guangjin Hou,Mark Walker,Ying Zhou,Zhen Shen,Jian Liu,Kun Qian,Wen Di
Chemotherapy is a primary cancer treatment strategy, the monitoring of which is critical to enhancing the survival rate and quality of life of cancer patients. However, current chemotherapy monitoring mainly relies on imaging tools with inefficient sensitivity and radiation invasiveness. Herein, we develop the bowl-shaped submicroreactor chip of Au-loaded 3-aminophenol formaldehyde resin (denoted as APF-bowl&Au) with a specifically designed structure and Au loading content. The obtained APF-bowl&Au, used as the matrix of laser desorption/ionization mass spectrometry (LDI MS), possesses an enhanced localized electromagnetic field for strengthened small metabolite detection. The APF-bowl&Au enables the extraction of serum metabolic fingerprints (SMFs), and machine learning of the SMFs achieves chemotherapy monitoring of ovarian cancer with area-under-the-curve (AUC) of 0.81–0.98. Furthermore, a serum metabolic biomarker panel is preliminarily identified, exhibiting gradual changes as the chemotherapy cycles proceed. This work provides insights into the development of nanochips and contributes to a universal detection platform for chemotherapy monitoring.
研究领域:
医学
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生物化学
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纳米技术
·
肿瘤学
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ACS Nano
·
2023
·
IF 17.1
Xiaohan Wang,Xiao Dai,Hao Wang,Jiong Wang,Qi Chen,Fengnan Chen,Qinghua Yi,Rujun Tang,Liang Gao,Liang Ma,Chen Wang,Xiangyi Wang,Guanglong He,Yue Fei,Yanqiu Guan,Biao Zhang,Yue Dai,Xuecou Tu,Lijian Zhang,Labao Zhang,Guifu Zou
Electron beam lithography uses an accelerated electron beam to fabricate patterning on an electron-beam-sensitive resist but requires complex dry etching or lift-off processes to transfer the pattern to the substrate or film on the substrate. In this study, etching-free electron beam lithography is developed to directly write a pattern of various materials in all-water processes, achieving the desired semiconductor nanopatterns on a silicon wafer. Introduced sugars are copolymerized with metal ions-coordinated polyethylenimine under the action of electron beams. The all-water process and thermal treatment result in nanomaterials with satisfactory electronic properties, indicating that diverse on-chip semiconductors (e.g., metal oxides, sulfides, and nitrides) can be directly printed on-chip by an aqueous solution system. As a demonstration, zinc oxide patterns can be achieved with a line width of 18 nm and a mobility of 3.94 cm2 V–1 s–1. This etching-free electron beam lithography strategy provides an efficient alternative for micro/nanofabrication and chip manufacturing.
研究领域:
微/纳米制造
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材料科学
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半导体技术
·
电子工程
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IEEE SENSORS JOURNAL
·
2025
·
IF 4.5
Ying Fang,Dehong Yang,Ziqi Wang,Xiaoyin Liu,Ruowang Dong,Jinbiao Ma,Jiahao Xu,Di Wang,Qingjun Liu,Fenni Zhang
Developing compact, sensitive, and fully integrated electrochemiluminescence (ECL) imaging systems is essential for advancing next-generation biosensors and point-of-care (POC) diagnostics. However, conventional ECL platforms typically require bulky, lens-based optical components to capture weak photon emissions, limiting their miniaturization and clinical utility. Here, we present a miniaturized, lensless on-chip ECL imaging platform (ECL-CMOS) that integrates electrochemical sensing directly with a CMOS imaging chip via contact-mode coupling. This architecture significantly enhances photon collection efficiency, expands the imaging field of view, and eliminates the need for external optics or alignment procedures. The system enables spatiotemporal detection of ECL signals from classical reaction pairs, including luminol/H₂O₂ and Ru(bpy)₃²⁺/TPrA, with high sensitivity and reproducibility. As a proof of concept for biomedical applicability, we demonstrate sensitive and quantitative glucose detection in both synthetic and human urine samples. The ECL-CMOS platform offers a scalable, cost-effective, and high-performance solution for real-time biochemical sensing, and holds strong potential for broad deployment in POC diagnostics, environmental analysis, and resource-limited settings.
研究领域:
生物医学工程
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分析化学
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即时检验
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分子诊断
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微流控技术
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生物传感器
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TALANTA
·
2025
·
IF 6.1
Chunjie Sun,Jiale Liu,Chengtao Xu,Zhiguang Tan,Haixia Yu,Dachao Li
As Type 2 Diabetes Mellitus (T2DM) has become one of the most severe public health burdens worldwide, it is of great importance to realize early screening and sensitive diagnosis. Adiponectin is widely recognized as a prominent biomarker of T2DM, which holds superior accuracy and sensitivity for early diagnosis owing to its inverse correlation in the reversible prodromal stage of the disease. This study presents a novel and reusable electrochemical immunosensor chip for adiponectin detection to facilitate early warning and intervention of T2DM. Adiponectin antibodies are immobilized on the working electrode, where the surface is modified by gold nanoparticles (AuNPs). The measurements show a high sensitivity of 3.03 μA/decade over 14 regeneration cycles, along with a broad linear range of 0.5–25,000 ng/mL and a low limit of detection (LOD) of 0.0093 ng/mL. Integrating the sensing unit into a flow cell not only enables low reagent consumption and continuous-flow operation, but also allows the immunosensor to demonstrate excellent repeatability, reproducibility, and strong anti-interference capability. Finally, the proposed immunosensor delivers precise measurements of real blood samples, which provides a foundation for future studies and practical applications in T2DM clinical diagnosis.
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Micromachines
·
2021
·Vol. 12
·期 9
·1034
·
IF 2.891
Lvhan Zhou,Qian Zhang,Xiangchun Xu,Xinming Huo,Qian Zhou,Xiaohao Wang,Quan Yu
The coupling of the microfluidic chip to mass spectrometry (MS) has attracted considerable attention in the area of chemical and biological analysis. The most commonly used ionization technique in the chip–MS system is electrospray ionization (ESI). Traditional chip-based ESI devices mainly employ direct electrical contact between the electrode and the spray solvent. In this study, a microchip ESI source based on a novel polarization-splitting approach was developed. Specifically, the droplet in the microchannel is first polarized by the electric field and then split into two sub-droplets. In this process, the charge generated by polarization is retained in the liquid, resulting in the generation of two charged droplets with opposite polarities. Finally, when these charged droplets reach the emitter, the electrospray process is initiated and both positive and negative ions are formed from the same solution. Preliminary experimental results indicate that the coupling of this polarization-splitting ESI (PS-ESI) chip with a mass spectrometer enables conventional ESI-MS analysis of various analytes.Keywords:microfluidic chip;mass spectrometry;electrospray ionization;induced polarization
研究领域:
分析化学
·
生物分析
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IONICS
·
2023
·Vol. 29
·期 7
·2911-2923
·
IF 2.8
Zhang Hao,Li Yudong,Han Enshan,Chen Gaojun,Zhang Ziqiang,Yan Caihong,He Yanzhen
The low electrical conductivity of nickel–cobalt layered oxides and single Ni, Co metal oxides led to the low rate capability and poor cycling stability, which thus limits the commercial application in practical supercapacitor. Here, NiCoO 2 /WCC composite electrode materials were prepared by employing wood chips carbon (WCC) with the high specific surface area and low cost as the conductive layer, where hierarchical spherical structure of laminar NiCoO 2 interspersed as homogeneous and was oriented and constructed on the surface of carbon by hydrothermal and calcination. The strategy provided abundant frameworks and active sites for the in situ growth of NiCoO 2 , which prevented the aggregation of spherical structures to a certain extent; furthermore, the layered spherical structure exposed more active sites, which thus enhanced the electrochemical performance of the capacitor. The NiCoO 2 /WCC electrode ( R Ni:Co = 1) possessed a high specific capacitance of 1053.6 F g −1 at 0.5 A g −1 due to the synergistic effect between the bimetallic oxide and WCC, and the specific capacitance of the electrode remained 906 F g −1 even at a high current density of 10 A g −1 by the lamellar structure with more electrochemical sites. In addition, the asymmetric supercapacitor based on the NiCoO 2 /WCC cathode and the WCC anode delivers a high specific capacitance of 134.4 F g −1 at 1 A g −1 , a high specific energy of 36.6 Wh kg −1 at 1 A g −1 , and good cycling performance (~ 94.3% retention after 5000 cycles), where the above properties were superior to existing and similar electrode materials.
研究领域:
材料科学
·
电化学
·
纳米技术
·
储能
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jianping Yang,Meiqiong Zheng,Fan Yang,Xiulian Zhang,Wei Yin,Xiaojun Liu,Guo-Jun Zhang,Zuanguang Chen
In this work, we have developed a novel pneumatic microfluidic chip (pChip) for in-vivo antibacterials screening at single-organism resolution using a P. aeruginosa-C. elegans infection model. To improve the accuracy and efficiency of nematode infection-based drug screening, we designed a pneumatic-controlled micro-sampler that enables simultaneous dispensing of a large number of individual free-moving worms into each culture chamber under physiological conditions without any mechanical squeezing, and the average probability of a single worm in a chamber is 88.5%, with RSD of 2.8%. Furthermore, we proposed an effective liquid P. aeruginosa - C. elegans infection model based on microfluidic chip, and the performance of the platform was verified with multiple clinical antibiotics. By applying this developed worm pChip to evaluate the antimicrobial activity of some natural medicinal compounds (coptisine, baicalin and gypenoside), we found that gypenoside can increase the P. aeruginosa infected worm survival by 2.3-fold at 30.0 μg/mL, whereas it does not suppress pathogen growth in vitro . It indicates that the well-established pneumatic chip will be an efficient platform for evaluation of antibacterial potential of medicinal natural compounds.
研究领域:
微生物学
·
药物开发
·
生物工程
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高影响因子
ACS Applied Energy Materials
·
2022
·
IF 6.959
Yanqiang Lei,Zihao Guo,Zhizhao Xu,Wenbin Guo,Zifeng Cong,Jinran Yu,Jia Sun,Chuankun Jia,Xiong Pu,Qijun Sun
Explosive progress in wireless and functional mobile electronics calls for miniaturized energy-storage units to push forward energy-autonomous and self-sustainable intelligent microsystems. Emerging three-dimensional (3D) microstructured electrodes for energy-storage devices have drawn great attention due to their attractive stereoscopic architectures to increase the areal loading of active materials. Here, we report a strategy for fabricating a 3D helical electrode for a zinc microbattery (Zn MB) by combining a “top-down” lithography technique and a “bottom-up” electrochemical depositing process. Thanks to the available stereoscopic space, the achieved 3D helical electrode shows a high surface area, outstanding electrolyte permeation, and stress adaptive capability. Based on the structural advantages of a 3D helical electrode, high-performance rechargeable Zn MB has been achieved by depositing Zn and NiOOH as anode and cathode materials, respectively. The Zn microbattery demonstrates a specific areal capacity up to 0.325 mAh·cm–2 corresponding to a high energy density of 0.55 mWh·cm–2. It also exhibits remarkable rate capability (0.272 mAh·cm–2 at 100 mA·cm–2) and excellent cycling stability (85% retention after 1000 cycles). The outstanding electrochemical performance indicates that the 3D Zn MB can work as a promising power source for advanced electronic devices. Paired micro-/nanofabrication process for battery electrodes is also of great significance in gearing toward carbon-neutral.
研究领域:
材料科学
·
能源技术
·
微纳米制造
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Liyan Bi,Huangruici Zhang,Chenyu Mu,Kaidi Sun,Hao Chen,Zhiyang Zhang,Lingxin Chen
High-speed and accuracy identification of pathogens has become increasingly critical in both individual patient care and public health. Artificial intelligence (AI)-assisted surface-enhanced Raman scattering (SERS) biosensors enable simultaneous identification of multiple pathogens. However, there are still problems such as low accuracy and limited diversity in bacterial fingerprints. To this end, we present a novel multi-branch adaptive attention convolutional neural network (MBAA-CNN)-assisted paper-based SERS chip for prompt and reliable pathogen discrimination. In the approach, we employed a dual-function molecule 4-mercaptophenylboronic acid (4-MPBA) to capture bacteria and enhance Raman spectra diversity, referring as 4-MPBA labeled mode (label mode). Meanwhile, we utilized the K -means algorithm to identify pathogens in the label mode, producing much higher accuracy compared to label-free mode ( n = 2000). Furthermore, we acquired 98.6 % accuracy at all pathogen species and 99.5 % accuracy at the antibiotic-resistant and sensitive strains ( n = 10,000) using MBAA-CNN. The superior performance of MBAA-CNN was further validated through comparisons with traditional machine learning models, particularly in terms of loss value, speed and accuracy. We envision the developed approach has potential for early culture-free diagnosis of pathogens and real-time monitoring of microbial contamination in water environment.
研究领域:
生物传感器技术
·
人工智能与医学
·
微生物学
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高影响因子
危险品化学品经营许可证（带存储）