Signal Transduction and Targeted Therapy
·
2026
·Vol. 11
·期 1
·177
·
IF 81.2
Wang Heru,Li Xiang,He Peng,Yang Sen,Liu Xiaoya,Zhu Qianhui,Liu Pan,Fang Xin,Wang Chenfei,Bi Ying,Jiang Gaojian,Sun Yufei,Chen Hongxu,Gao Tie,Luo Ji,Rong Huan,Liu Xiaosa,Wang Xiangxi,Zhang Yuxia,Hu Zhongyu
Glycosylation plays a pivotal role in modulating the structure and immunogenicity of viral antigens. Three glycosylation sites on the receptor-binding domain (RBD) of SARS-CoV-2, including N331, N343 (N-linked), and T323 (O-linked), are highly conserved and remain unchanged across multiple variant strains. To investigate their functional relevance, a series of site-directed glycosylation-deficient mutants were generated on the basis of the RBD-dimer antigen of the ZF2001 vaccine (developed by ZFSW Biologics), including N-N331-NA, N-N343-NA, O-T323-NA, and an RBD-NA control. Comparative analyses of glycosylation-deficient RBD variants revealed a site-specific hierarchy in immune modulation. In particular, disruption of the N343 site led to a pronounced reduction in antigen-specific antibody titers and T helper cell-related cytokine responses in immunized mice, whereas mutations at N331 and T323 had more limited effects. Furthermore, glycan profiling using liquid chromatography–tandem mass spectrometry (LC–MS/MS), secondary structure assessment using microfluidic modulation spectroscopy (MMS), and molecular dynamics simulations revealed that the N343 glycan contributes to local structural stability and preserves key antigenic features of the RBD. Together, these results identify N343 as a critical glycosylation hotspot that governs RBD immunogenicity and antigenicity, providing a mechanistic foundation for the structure-based optimization of SARS-CoV-2 vaccines targeting glycan-regulated epitopes.
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International Journal of Environmental Research and Public Health
·
2023
·Vol. 20
·期 5
·4474
·
IF 4.614
Anahita Ali,Santosh Kumar
Healthcare workers (HCWs) including doctors, nurses and allied workers struggled to cope up with the stressful situation as the COVID-19 pandemic unsettled healthcare systems, including India’s. Many factors (commonly called as stressors) acted as major sources of stress and resulted in poor mental health of HCWs. Therefore, this study predicted and explained the mediating effect of challenges on demographic characteristics and coping strategies of HCWs. Data from a cross-sectional study was collected from the district hospital of Rajasthan, India, during the period of August 2022–October 2022. HCW’s experience level, shift type and distance of greenspaces from their accommodation were significantly correlated with the challenges they faced at work, specifically societal challenges. Thus, HCWs were more inclined to adopt a meaning-focused coping strategy to retain good mental health during the pandemic. Therefore, these findings call for interventions requiring a layered response, comprising strategies and actions that are structural. At the organizational level, these actions may provide supportive workplace environments.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2023
·
IF 8.7
Ziwei Guo,Huan He,Kunqian Liu,Shicheng Yang,Zihui Li,Chaochao Lai,Zhicheng Liao,Xiaomin Ren,Bin Huang,Xuejun Pan
Insights into COVID-19 antivirals’ environmental fate and ecological risk are urgently required due to their increasing concentrations in aquatic environments, which have rarely been studied. Herein, we first investigated the photochemical transformation and the resulting alterations in toxicity of arbidol, an antiviral drug with relatively higher toxicity. The photolysis of arbidol was rapid with a rate constant of 0.106 min −1 due to its superior ultraviolet light absorption , in which the direct photolysis was predominated with a contribution of 91.5%. Despite its substantial photolysis, only 14.45% of arbidol was mineralized after 100 min, implying that arbidol and its products might have a long-term impact on aquatic environment. It was inferred that arbidol was photolyzed mainly via the loss of thiophenol, bromine, and alkylamine, based on twelve photolytic products identified. Notably, the experimental results demonstrated that the photolysis process increased the acute toxicity of arbidol, and the toxicity prediction indicated that the ecotoxicity of two photolytic products was very high with LC 50 values below 0.1 mg/L. Due to the co-effect of multiple constituents, the photolytic rate observed in wastewater treatment plant effluent and in river water was comparable to that in ultra-pure water, while it was slightly enhanced in lake water. The presence of dissolved organic matter suppressed arbidol photolysis, while NO 3 − exhibited a promotion effect. These results would be of great significance to assess the fate and risk of COVID-19 antivirals in natural aquatic environments.
研究领域:
环境科学
·
毒理学
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公共卫生
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yongsheng Ni,Jingyao Wang,Mengyi Wang,Lizhi Liu,Hongqing Nie,Qiaoling Wang,Jing Sun,Tianli Yue,Ming-Qiang Zhu,Jianlong Wang
COVID-19 threatens human life because of the super destructiveness produced from its coronal morphology and strong transmembrane infection based on spike glycoprotein. Inspired by the coronal morphology of COVID-19 and its means of infecting, we designed an “artificial virus” with coronal morphology based on the concept of “defeating superbacteria with superviruses” by self-assembling a transacting activator of transduction peptide with triple-shell porous graphitic carbon nitride (g-C 3 N 4 ) embedded with cobalt nanoparticles to forcefully infect methicillin-resistant Staphylococcus aureus (MRSA). The results confirmed that this “artificial virus” had unique properties of crossing the bacterial cell membrane barrier, heating the internal bacterial microenvironment and triggering ROS outbreak, based on its coronal morphology, membrane penetration, temperature-rising and heat insulation, oxidase-like activity and excellent visible-light harvesting properties. It had a high sterilization efficiency of 99.99% at 20 min, which was 18.6 times that of g-C 3 N 4 , and the efficiency remained at 99.99% after 3 rounds of recycling and reuse. Additionally, it can rapidly inactivate bacteria in river water and accelerate wound healing.
研究领域:
生物学
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医学
·
纳米医学
·
抗菌研究
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Liu Shi,Lin Wang,Xuemei Ma,Xiaona Fang,Liangliang Xiang,Yongxiang Yi,Jinlong Li,Zhaofeng Luo,Genxi Li
With the outbreak of COVID-19, which is fast transmitting and highly contagious, the development of rapid, highly specific, and sensitive detection kits has become a research hotspot. The existing assay methods for SARS-CoV-2 are mainly based on enzymatic reactions, which require expensive reagents, hindering popular use, especially in resource-constrained areas. Herein, we propose an aptamer-based method for the assay of SARS-CoV-2 via binding of the spike protein using functionalized biomimetic nanochannels. To get the analogous effect of human ACE2, a receptor for the spike protein, the aptamer to bind to the spike S1 protein has been first screened by a SELEX technique and then immobilized on the previously prepared nanochannels. In the presence of SARS-CoV-2, the changes in steric hindrance and charge density on the surface of the nanochannels will affect the ion transport, along with a rapid electrochemical response. Our method has been successfully applied to detect the viral particles in clinical pharyngeal swab specimens in one step without sample treatment. We expect this rapid, reagent-free, and sensitive assay method to be developed as a useful tool for diagnosing COVID-19.
研究领域:
生物医学工程
·
病毒学
·
诊断学
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Lujian Lin,Bo Yuan,Hualong Hong,Hanyi Li,Le He,Haoliang Lu,Jingchun Liu,Chongling Yan
With the outbreak and widespread of the COVID-19 pandemic, large numbers of disposable face masks (DFMs) were abandoned in the environment. This study first investigated the sorption and desorption behaviors of four antibiotics (tetracycline (TC), ciprofloxacin (CIP), sulfamethoxazole (SMX), and triclosan (TCS)) on DFMs in the freshwater and seawater. It was found that the antibiotics in the freshwater exhibited relatively higher sorption and desorption capacities on the DFMs than those in the seawater. Here the antibiotics sorption processes were greatly related to their zwitterion species while the effect of salinity on the sorption processes was negligible. However, the desorption processes were jointly dominated by solution pH and salinity, with greater desorption capacities at lower pH values and salinity. Interestingly, we found that the distribution coefficient ( K d ) of TCS (0.3947 L/g) and SMX (0.0399 L/g) on DFMs was higher than those on some microplastics in freshwater systems. The sorption affinity of the antibiotics onto the DFMs followed the order of TCS > SMX > CIP > TC, which was positively correlated with octanol-water partition coefficient (log K ow ) of the antibiotics. Besides, the sorption processes of the antibiotics onto the DFMs were mainly predominated by film diffusion and partitioning mechanism. Overall, hydrophobic interaction regulated the antibiotics sorption processes. These findings would help to evaluate the environmental behavior of DFMs and to provide the analytical framework of their role in the transport of other pollutants.
研究领域:
环境科学
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水质
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污染控制
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化学工程
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BMC INFECTIOUS DISEASES
·
2022
·Vol. 22
·期 1
·1-8
·
IF 3.667
An Qingyu,Wu Jun,Bai Jin jian,Li Xiaofeng
Objectives To forecast the development trend of current outbreak in Dalian, mainly to predict turning points of COVID-19 outbreak in Dalian, Liaoning province, China, the results can be used to provide a scientific reference for timely adjustment of prevention and control strategies. Methods During the outbreak, Bayesian framework was used to calculated the time-dependent reproduction number ( \({R_{t}}\) ), and then above acquired \({R_{t}}\) and exponential trend equation were used to establish the prediction model, through the model, predict the \({R_{t}}\) value of following data and know when \({R_{t}}\) smaller than 1. Results From July 22 to August 5, 2020, and from March 14 to April 2, 2022, 92 and 632 confirmed cases and asymptomatic infected cases of COVID-19 were reported (324 males and 400 females) in Dalian. The R square for exponential trend equation were 0.982 and 0.980, respectively which fit the \({R_{t}}\) with illness onset between July 19 to July 28, 2020 and between March 5 to March 17, 2022. According to the result of prediction, under the current strength of prevention and control, the \({R_{t}}\) of COVID-19 will drop below 1 till August 2, 2020 and March 26, 2022, respectively in Dalian, one day earlier or later than the actual date. That is, the turning point of the COVID-19 outbreak in Dalian, Liaoning province, China will occur on August 2, 2020 and March 26, 2022. Conclusions Using time-dependent reproduction number values to predict turning points of COVID-19 outbreak in Dalian, Liaoning province, China was effective and reliable on the whole, and the results can be used to establish a sensitive early warning mechanism to guide the timely adjustment of COVID-19 prevention and control strategies.
研究领域:
流行病学
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公共卫生
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传染病学
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生物统计学
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预测模型
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疫情防控
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GENDER WORK AND ORGANIZATION
·
2021
·Vol. 28
·期 5
·1876-1884
·
IF 3.465
Yuchen Han
This article documents the reconstructed domestic masculinity of a retired Chinese man during the COVID-19 quarantine period in China. It is based on participant observation of the man and his family as a case study. It demonstrates how the man turns kitchen work into a “masculine” job, and uses it as a contested terrain for constructing hegemonic masculinity by adopting scientific discourse explicitly and traditional patriarchal discourse implicitly. It also highlights women's conscious and deliberate interactions with the man in contributing to the making of hegemonic masculinity for the sake of their own values of happiness. The author seeks an understanding of the intersection of aging, patriarchal norms, and women's agency through the case of the co-production of a retired man's hegemonic masculinity in the context of the COVID-19 quarantine.
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Viruses-Basel
·
2026
·Vol. 18
·期 9
·976
·
IF 3.8
Zikang Wang,Jiahua Gao,Ruojing Bai,Lunzhi Yuan,Huilin Guo,Shaojuan Wang,Youfeng Wang,Jiayi Wu,Qingfang Bu,Huiyu Guo,Yunda Hong,Jian Ma,Kai Wang,Wenjie Guo,Yanyan Liu,Zhitao Weng,Tianying Zhang,Ningshao Xia,Quan Yuan,Yali Zhang,Yangtao Wu
The continued evolution of SARS-CoV-2 has reduced the protective effectiveness of first-generation vaccines and underscores the need for broadly reactive next-generation vaccine candidates. Here, we evaluated STFKB, an alum-adjuvanted bivalent recombinant protein vaccine composed of the monomeric Spike (STFKprototype) and the engineered Spike (STFK1628X). We assessed the immunogenicity, tolerability, and protective efficacy of STFKB in mice, rats, guinea pigs, rhesus macaques, and Syrian hamsters. STFKB induced robust STFK- and STFK1628X-specific antibody responses and broadly reactive neutralizing antibodies against multiple SARS-CoV-2 variants in the tested animal models. In hamster challenge studies, STFKB vaccination protected animals from Omicron BA.1 and BA.5 challenge, as shown by reduced body-weight loss, lower viral RNA loads in respiratory tissues, and improved gross lung pathology. Across the tested preclinical models, STFKB was well tolerated, with no vaccine-related overt toxicity observed under the study conditions. These findings support the rationale and translational potential of the bivalent vaccine strategy proposed in this study.
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THERAPEUTIC DRUG MONITORING
·
2025
·
IF 2.4
Ma Zhiyuan,Bai Mengru,Shen Shuying,Zhou Junshan,Dong Rong,Zhang Jiangjun,Weng Yayun,Li Li,Li Yongchen,Liu Dan,Yan Wei,Lin Nengming,Xia Jianmei
Background: Nirmatrelvir/ritonavir is licensed for the treatment of mild-to-moderate coronavirus disease (COVID-19) in patients at an increased risk of progression to severe disease. However, data on the real-world plasma exposure to nirmatrelvir/ritonavir remain limited, particularly in Chinese patients. This study aimed to assess the nirmatrelvir/ritonavir trough concentration (C trough ) and identify its critical factors in hospitalized Chinese patients treated with nirmatrelvir/ritonavir 300 mg/100 mg twice daily over a 5-day course. Methods: A high-performance liquid chromatography-tandem mass spectrometry assay was developed and validated to measure the nirmatrelvir/ritonavir C trough . Correlation analyses were performed to identify the variables influencing nirmatrelvir/ritonavir C trough . Results: Among the 110 patients, 100% had plasma concentrations above the antiviral in vitro 90% effective concentration. The median C trough of nirmatrelvir was 4.55 mcg/mL (15.6× 90% effective concentration), ranging from 0.65 to 12.44 mcg/mL. Nirmatrelvir C trough in normal and mild renal impairment cohorts were comparable (4.09 ± 1.97 mcg/mL and 4.57 ± 2.21 mcg/mL) but significantly increased in the moderate renal impairment cohort (6.41 ± 2.31 mcg/mL). Sex, age, and obesity were not significantly associated with nirmatrelvir exposure. Conclusions: Nirmatrelvir C trough was high in Chinese patients with COVID-19, and therapeutic drug monitoring should not be routinely recommended, except in patients with renal impairment.
研究领域:
药理学
·
传染病
·
临床研究
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Hui Zhao,Feng Liu,Wei Xie,Tai-Cheng Zhou,Jun OuYang,Lian Jin,Hui Li,Chun-Yan Zhao,Liang Zhang,Jia Wei,Ya-Ping Zhang,Can-Peng Li
The recent pandemic outbreak of COVID-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to public health globally. Thus, developing a rapid, accurate, and easy-to-implement diagnostic system for SARS-CoV-2 is crucial for controlling infection sources and monitoring illness progression. Here, we reported an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting RNA of SARS-CoV-2. Based on a supersandwich-type recognition strategy, the technology was confirmed to practicably detect the RNA of SARS-CoV-2 without nucleic acid amplification and reverse-transcription by using a portable electrochemical smartphone. The biosensor showed high specificity and selectivity during in silico analysis and actual testing. A total of 88 RNA extracts from 25 SARS-CoV-2-confirmed patients and eight recovery patients were detected using the biosensor. The detectable ratios (85.5 % and 46.2 %) were higher than those obtained using RT-qPCR (56.5 % and 7.7 %). The limit of detection (LOD) of the clinical specimen was 200 copies/mL, which is the lowest LOD among the published RNA measurement of SARS-CoV-2 to date. Additionally, only two copies (10 μL) of SARS-CoV-2 were required for per assay. Therefore, we developed an ultrasensitive, accurate, and convenient assay for SARS-CoV-2 detection, providing a potential method for point-of-care testing.
研究领域:
生物技术
·
电化学
·
即时诊断
·
病毒学
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2023
·
IF 5.4
Lan-Yi Hu,Ye Yuan,Zi-Xuan Wen,Yi-Yue Hu,Miao-Miao Yin,Yan-Jun Hu
Chloroquine (CQ) and hydroxychloroquine (HCQ) show good efficacy in the treatment of SARS-CoV-2 in the early stage, while they are no longer recommended due to their side effects. As an important drug delivery carrier, serum albumin (SA) is closely related to the efficacy of drugs . Here, the affinity behaviour of chloroquine and hydroxychloroquine with two SA were investigated through the multispectral method of biochemistry and computer simulation. The results showed that the intrinsic emission of both SA was quenched by CQ and HCQ in a spontaneous exothermic entropy reduction static process, which relied mainly on hydrogen bonding and van der Waals forces . The lower binding constants suggested weak binding between the two drugs and SA, which might lead to differences in efficacy and possibly even to varying side effects. Binding site recognition demonstrated that CQ preferred to bind to the two sites of both SA, while HCQ tended to bind to site I of SA. The results of conformational studies demonstrated that CQ and HCQ could affect the structure of both SA by slightly increasing the α -helix content of SA. Finally, we combine the results from experimental start with molecular simulations to suggest drug modifications to guide the design of drugs. This work has important implications for guiding drug design improvements to select CQ derivatives with fewer side effects for the treatment of COVID-19.
研究领域:
药理学
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生物化学
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分子生物学
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病毒学
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