CHEMOSPHERE
·
2022
·
IF 8.943
Yingjie Liu,Zhongxiang Chen,Shanwei Li,Lu Ding,Xiaofeng Wei,Shicheng Han,Peng Wang,Yanchun Sun
Di-(2-ethylhexyl) phthalate (DEHP) is one of the most extensively utilized plasticizers in the plastic manufacturing process. It is widely used in various fields due to its low cost and excellent effect. Although there is evidence that DEHP is harmful to animal and human health, DEHP-induced gill toxicity in aquatic organisms is inconclusive, and its mechanism has not been fully elucidated. Here, we investigated the effects of DEHP acute exposure on crucian carp gills at environmentally relevant concentrations of 20, 100, and 500 μg/L. Multi-omics profiling and biochemical assays were employed to characterize the potential toxicological mechanisms. The results showed that acute exposure to 100 and 500 μg/L of DEHP leads to oxidative stress in gills, as evidenced by overproduction of reactive oxygen species (ROS), increased antioxidant enzyme activity , and the transformation of glutathione from reduced to oxidized form, resulting in lipid peroxidation. Integrative analysis of transcriptomics and metabolomics indicated that increased purine metabolism was the potential source of increased ROS. Moreover, lipid metabolism disorder, including arachidonic acid metabolism, induces inflammation. Further, DEHP causes the imbalance of the CYP enzyme system in the gill, and DEHP-induced gill toxicity in crucian carp was associated with interference with CYP450 homeostasis . Taken together, this study broadens the molecular understanding of the DEHP-induced gill toxicity in aquatic organisms and provides novel perspectives for assessing the effects of DEHP on target and non-target aquatic organisms in the environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Yang Yang,Yingjie Liu,Song Wu,Lin Han,Yanchun Sun
The pervasive use of the plasticizer di(2-ethylhexyl) phthalate (DEHP) poses potential risks to global aquatic ecosystems. This study systematically evaluated the adverse effects of chronic exposure to environmentally relevant concentrations of DEHP on gill tissues of crucian carp, utilizing histological examination, metabolomic, and transcriptomic analysis. The results demonstrated that DEHP induced significant histopathological alterations in gill tissues, with significant enrichment observed in multiple pathways associated with amino acid, hormone, lipid, and xenobiotic metabolism. Metabonomics-transcriptomics analyses indicated that DEHP-induced significantly over-activation of cytochrome P450 1B1-like ( p < 0.001 ) and cytochrome P450 3A30-like ( p < 0.05 ) via the nuclear xenobiotic receptors pathway was a key factor contributing to the disruption of tryptophan metabolism and steroid hormone biosynthesis, as well as inducing circadian rhythm disruption. Moreover, circadian rhythm disruption further exacerbated the imbalance of cytochrome P450 (CYP450) enzyme system as well as linoleic acid, arachidonic acid, sphingolipid, and glycerophospholipid metabolism. Overall, the feedback regulation between the CYP450 enzyme system and circadian rhythms emerged as the primary mechanism underlying DEHP-induced metabolic and transcriptional disruptions, ultimately resulting in gill toxicity. This study not only enriched the toxic effects on aquatic organisms of chronic exposure to DEHP, but provided potential biomarkers for the environmental risk assessment of DEHP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 15
·期 1
·5
·
IF 3.4
Shicheng Han,Lin Han,Fangying Yuan,Wenzhi Liu,Jing Wang,Xiaofeng Jin,Yanchun Sun
Background : Owing to the progressive rise in saline waters globally, resulting in detrimental impacts on freshwater aquaculture, the underlying molecular distinctions governing the response to alkaline stress between diploid and triploid crucian carp remain unknown. Methods : This investigation explores the effects of 20 and 60 mmol NaHCO 3 stress over 30 days on the gills of diploid and triploid crucian carp, employing histological, biochemical, and multi-omic analyses. Results : Findings reveal structural damage to gill lamellas in the examined tissue. Diploid crucian carp exhibit heightened activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and alkaline phosphatase (AKP), alongside lower malondialdehyde (MDA) and urea nitrogen (BUN) levels compared to triploid counterparts. Metabolomic investigations suggest alterations in purine metabolism, lipid metabolism, sphingolipid metabolism, and aminoglycan and nucleotide sugar metabolism following NaHCO 3 exposure. Transcriptomic data indicate differential expression of genes associated with nitrogen metabolism, complement and coagulation cascades, IL-17 signaling pathways, and Toll-like receptor signaling pathways. Conclusions : Overall, NaHCO 3 -induced stress leads to significant gill tissue damage, accompanied by reactive oxygen species (ROS) production causing oxidative stress and disruptions in lipid metabolism in crucian carp. Furthermore, an inflammatory response in gill cells triggers an immune response. Diploid crucian carp exhibit superior antioxidant and immune capacities compared to triploid counterparts, while also displaying reduced inflammatory responses in vivo. Notably, diploid carp efficiently excrete excess BUN through purine metabolism, mitigating protein metabolism and amino acid imbalances caused by BUN accumulation. This enables them to allocate less energy for coping with external environmental stress, redirecting surplus energy toward growth and development. The above results indicate that diploid organisms can better adapt to saline–alkaline environments. Overall, this study provides novel perspectives into species selection of crucian carp of different ploidy in saline–alkaline waters.
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE RESEARCH
·
2019
·Vol. 50
·期 11
·3138-3153
·
IF 1.502
Xiaohuan Cao,Yajun Wang,Na Yu,Qijun Le,Jiabao Hu,Yang Yang,Siwen Kuang,Man Zhang,Yibo Sun,Weiwei Gu,Xiaojun Yan
Anaesthetics are widely used in aquaculture as a method of stress relief. However, few studies have reported the influence of anaesthetics on the immune system of fish. Therefore, in the present study, we selected two different anaesthetics, MS-222 (100 mg/L) and eugenol (20 mg/L), to anaesthetize crucian carp and analyse the transcriptome. It was found that over 137 million high-quality reads were generated and de novo assembled into a final set of 97,556 unigenes. A total of eight differentially expressed genes (DEGs) related to the immune response were co-expressed in the two anaesthetic groups. GO and the KEGG revealed that these genes functioned primarily to enrich antigen processing and presentation pathways, including MHCI , MHCIα , p-MHCIIα , P-MHCIIα , CD74 , MHCII antigen and MHCII antigenα . Moreover, LRRFIP2 was found be associated with the immune response. Consistent with the transcriptome findings, qPCR verified the changes in the relative level of expression of these genes. Furthermore, long-term exposure to low concentrations of MS-222 (30 mg/L) and eugenol (8 mg/L) showed an impact on these immune genes. In conclusion, anaesthetics used in high or low concentrations for treatment and transport could affect the immune system in fish species. While eugenol was associated with an earlier activation of immune gene expression, MS-222 exhibited more significant effects on the immune response. These findings improve the understanding of the mechanisms of anaesthetics on immune damage and will be of great value for future studies involving anaesthetic selection and treatment for fish.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）