Bioactive Materials
·
2025
·
IF 20.3
Jiayi Shi,Xuekai Qi,Ying Ran,Qiang Zhou,Yiqin Ding,Lujian Li,Youyun Zeng,Dongchao Qiu,Zhibin Cai,Xiaojun Cai,Yihuai Pan
Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues. Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity. According to the Interrupting Dental Caries (IDC) theory, ideal caries-preventive materials should possess both antibacterial and remineralizing properties. Furthermore, effective adhesion to dental surfaces is crucial. Inspired by the wet adhesion properties of the salivary acquired pellicle, we developed a multifunctional gargle named Ce6@PDN-SAP (CP-SAP). This formulation employed peptide dendrimer nanogels (PDN) as a carrier for the photosensitizer Ce6, further functionalized with saliva-acquired peptide (SAP) to confer wet adhesion properties. CP-SAP rapidly adhered to the dental surface and remained effective for extended periods. Upon laser irradiation, Ce6 generated reactive oxygen species (ROS), disrupting bacterial outer membrane integrity, causing protein leakage, and reducing ATP levels, thereby achieving potent antibacterial effects. Following this, PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues. In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle. In conclusion, CP-SAP, as an innovative approach grounded in the IDC theory, holds great promise for the prevention and treatment of dental caries.
研究领域:
牙科学
·
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiawen Chen,Hong Zhang,Tiancong Zhao,Yiyan Yu,Jiazhuo Song,Yuanhang Zhao,Hamed Alshawwa,Xinying Zou,Zhimin Zhang
The successful treatment of persistent and recurrent endodontic infections hinges upon the eradication of residual microorganisms within the root canal system, which urgently needs novel drugs to deliver potent yet gentle antimicrobial effects. Antibacterial photodynamic therapy (aPDT) is a promising tool for root canal infection management. Nevertheless, the hypoxic microenvironment within the root canal system significantly limits the efficacy of this treatment. Herein, a nanohybrid drug, Ce6/CaO 2 /ZIF-8@PEI, is developed using a bottom-up strategy to self-supply oxygen for enhanced aPDT. Polyethylenimine (PEI) provides a positively charged surface, which enables precise targeting of bacteria. CaO 2 reacts with H 2 O to generate O 2 , which alleviates the hypoxia in the root canal and serves as a substrate for Ce6 under 660 nm laser irradiation, leading to the successful eradication of planktonic Enterococcus faecalis ( E. faecalis) and biofilm in vitro and, moreover, the effective elimination of mature E. faecalis biofilm in situ within the root canal system. This smart design offers a viable alternative for mitigating hypoxia within the root canal system to overcome the restricted efficacy of photosensitizers, providing an exciting prospect for the clinical management of persistent endodontic infection. This article is protected by copyright. All rights reserved
研究领域:
牙科学
·
纳米技术
·
光疗
·
微生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 4
·743-751
·
IF 4.726
Ya Wen,Haiqing Dong,Yan Li,Aijun Shen,Yongyong Li
Biomineralization of a rare earth ion (Gd) is first employed to assemble bovine serum albumin (BSA) into sub-50 nm nanoparticles (Gd@BSA) for theranostic applications, via a straightforward and reproducible strategy. Combination of Gd ions with BSA under mild conditions results in the formation of the Gd@BSA nanosystem, which has been used as the scaffold material to encapsulate a photosensitiser (Ce6) with high efficiency up to 20 wt%. Beyond playing an important role in the assembled process, the incorporation of Gd affords the potential MRI and fluorescence imaging capability of Gd@BSA-Ce6 nanoparticles. In vivo MRI allowed real-time imaging in tumor-bearing mice and showed advantages in terms of circulation time, compared with commercially used Gd–DTPA. Gd@BSA-Ce6 nanoparticles exhibited enhanced tumor-specific distribution, through enhanced permeability and retention effect, and complete cure of tumor-bearing mice after intravenous injection. The nanoparticles did not produce systemic toxicity as revealed by biodistribution and histology toxicity analyses. The results demonstrate the great potential of Gd@BSA-Ce6 nanoparticles as theranostic agents due to their excellent imaging and tumor-growth-inhibition properties.
研究领域:
纳米医学
·
癌症治疗
·
分子影像
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Fang Ning,Dengshuai Wei,Hongli Yu,Tingting Song,Zhipeng Li,Hongmei Ma,Yong Sun
Inhibition of autophagy increases the sensitivity of tumor cells to radiotherapy and chemotherapy and improves the therapeutic effect on tumors. Recently, photodynamic therapy (PDT) combined with chemotherapy has been proven to further improve the efficiency of cancer treatment. As such, combining autophagy inhibition with PDT and chemotherapy may represent a potentially effective new strategy for cancer treatment. However, currently widely studied autophagy inhibitors inevitably produce various toxic side effects due to their inherent pharmacological activity. To overcome this constraint, in this study, we designed an ideal multifunctional upconversion nanoplatform, UCNP-Ce6-EPI@mPPA + NIR (MUCEN). Control, UCNP-EPI@mPPA (MUE), UCNP-EPI@mPPA + NIR (MUEN), Ce6-EPI@mPPA (MCE), Ce6-EPI@mPPA + NIR (MCEN), and UCNP-Ce6-EPI@mPPA (MUCE) groups were set up separately as controls. Based on a combination of autophagy inhibition and PDT, the average particle size of MUCEN was 197 nm, which can simultaneously achieve the double encapsulation of chlorine e6 (Ce6) and epirubicin (EPI). In vitro tests revealed that MUCE was efficiently endocytosed by 4T1 cells under near-infrared light irradiation. Further, in vivo tests revealed that MUCE dramatically inhibited tumor growth. Immunohistochemistry results indicated that MUCE efficiently increased the expression of autophagy inhibitors p62 and LC3 in tumor tissues. The synergistic effect of autophagy inhibition and PDT with MUCE exhibited superior tumor suppression, providing an innovative approach to cancer treatment.
研究领域:
纳米技术
·
癌症研究
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2021
·Vol. 7
·期 12
·1361-1368
·
IF 3.154
Zhaoyu Han,Yue Qian,Yiyang Wu,Yanfei Cai,Jian Jin,Zhaoqi Yang
Graphical A hybrid drug delivery system based on ZIF-90 encapsulated with Doxorubicin and Ce6-G3139 has been established to induce apoptosis for enhanced chemotherapy. Metal-organic frameworks are highly porous and biodegradable and have found numerous applications in biomedicine. However, the traditional chemotherapy of MOF-drug delivery system remains a great challenge because of adverse side effects and cell tolerance. In this work, we have fabricated a hybrid drug delivery system based on ZIF-90 encapsulated with Doxorubicin (DOX) and Ce6-G3139 for enhanced chemotherapy. The released DOX can enter cell nucleus and trigger cell death. In addition, the G3139 binds to the anti-apoptotic gene Bcl-2 in tumor cells and related proteins are downregulated to inhibit cell proliferation. Meanwhile, the photosensitizer Ce6 carried by the nucleic acid will generate abundant reactive oxygen species under a near-infrared (NIR) laser irradiation thus induces more cell apoptosis. It was worth mentioning that in vitro MTT test in the MCF-7 cells assays revealed that DOX@ ZIF-90/Ce6-G3139 nanoparticles possessed stronger anticancer capability than free DOX or DOX@ ZIF-90 nanoparticles indicating that the investigation offers an exciting new therapeutic strategy for the treatment of chemotherapy.
研究领域:
生物医学工程
·
癌症治疗
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纳米技术
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药学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Liu Wang,Yangyi Liu,Jing Sun,Juanjuan Su,Jing Feng,Li Miao,Weijie Zhao,Hongjie Zhang,Kai Liu
Sono-photodynamic therapy (SPDT) integrates the advantages of sonodynamic therapy (SDT) and photodynamic therapy (PDT), showing great potential for the minimally invasive treatment of malignant tumors. Nevertheless, the effectiveness of sensitizers in PDT is often limited by insufficient light penetration, restricting the synergistic therapeutic effects of SPDT. Herein, we present a controllable strategy using in situ ultrasound-triggered bioluminescence for enhanced SPDT. This method utilizes a mechanosensitive bioluminescent protein composed of the mechanosensitive channel of large conductance (MscL) and the aequorin (AEQ) photoprotein. Upon ultrasound stimulation, MscL responds to mechanical force by inducing Ca2+ influx, which subsequently activates AEQ to oxidize a luminogenic substrate and generate bioluminescence. By leveraging ultrasound and its associated bioluminescence, the natural sono-photosensitizer, Chlorin e6 (Ce6), exhibits efficient sono-/photocytotoxicity, induces immunogenic cell death, and synergistically enhances the antitumor immune response. This ultrasound-triggered SPDT not only suppresses primary tumor growth and metastasis but also induces a long-term immune memory to resist tumor rechallenge. The integration of SPDT with immune activation forms a multimodal “sono–photo–immuno” platform for in situ breast tumor treatment and simultaneously prevents tumor recurrence after tumor resection. These findings showcase our platform as a versatile tool for the development of more effective cancer therapies.
研究领域:
肿瘤学
·
生物工程
·
生物光子学
·
免疫治疗
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分子成像
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诊疗一体化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Xinying Yu,Bei Li,Jie Yan,Wenxi Li,Hao Tian,Guohao Wang,Songtao Zhou,Yunlu Dai
Proteotoxic stress, caused by the accumulation of abnormal unfolded or misfolded cellular proteins, can efficiently activate inflammatory innate immune response. Initiating the mitochondrial proteotoxic stress might go forward to enable the cytosolic release of intramitochondrial DNA (mtDNA) for the immune-related mtDNA–cGAS–STING activation, which however is easily eliminated by a cell self-protection, i.e., mitophagy. In light of this, a nanoinducer (PCM) is reported to trigger mitophagy-inhibited cuproptotic proteotoxicity. Through a simple metal-phenolic coordination, PCMs reduce the original Cu 2+ with the phenolic group of PEG-polyphenol-chlorin e6 (Ce6) into Cu + . Cu + thereby performs its high binding affinity to dihydrolipoamide S-acetyltransferase (DLAT) and aggregates DLAT for cuproptotic proteotoxic stress and mitochondrial respiratory inhibition. Meanwhile, intracellular oxygen saved from the respiratory failure can be utilized by PCM-conjugated Ce6 to boost the proteotoxic stress. Next, PCM-loaded mitophagy inhibitor (Mdivi-1) protects proteotoxic products from being mitophagy-eliminated, which allows more mtDNA to be released in the cytosol and successfully stimulate the cGAS–STING signaling. In vitro and in vivo studies reveal that PCMs can upregulate the tumor-infiltrated NK cells by 24% and enhance the cytotoxic killing of effector T cells. This study proposes an anti-tumor immunotherapy through mitochondrial proteotoxicity.
研究领域:
癌症生物学
·
免疫学
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 4
·1018-1029
·
IF 5.344
Tianren Xu,Xiaojiao Zhu,Li Yang,Yingcui Bu,Yuyang Zhang,Jie Zhang,Lianke Wang,Zhipeng Yu,Hongping Zhou
Recently, phototherapy has attracted much attention due to its negligible invasiveness, insignificant toxicity and excellent applicability. The construction of a newly proposed nanosystem with synergistic photothermal and photodynamic tumor-eliminating properties requires a delicate structure design. In this work, a novel therapeutic nanoplatform (denoted as BCS-Ce6) based on defective cobalt hydroxide nanosheets was developed, which realized hypoxia-relieved photothermal-enhanced photodynamic therapy against cancer. Defective cobalt hydroxide exhibited high photothermal conversion efficacy at the near-infrared region (49.49% at 808 nm) as well as enhanced catalase-like activity to produce oxygen and greatly boost the singlet oxygen generation by a photosensitizer, Ce6, realizing efficacious dual-modal phototherapy. In vivo and in vitro experiments revealed that BCS-Ce6 can almost completely extinguish implanted tumors in a mouse model and present satisfactory biocompatibility during the treatment. This work sets a new angle of preparing photothermal agents and constructing comprehensive therapeutic nanosystems with the ability to modulate the hypoxic tumor microenvironment for efficient cancer therapy.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2023
·
IF 5.4
Binbin Liang,Yuhang Miao,Liying Zhao,Lan Fang,Dawei Deng
The synergistic effect of chemotherapy and photo-dynamic therapy (PDT) is an effective way to improve the efficiency of tumor treatment . However, most synergistic therapeutic drugs have poor water solubility and stability, so it is difficult to achieve high therapeutic effects while avoiding the severe side effects. Herein, a unique dandelion-like nanomedicine (named as cRGDfk-CCPT-mCe6) was successfully synthesized using Ce6-loaded amphiphilic β-cyclodextrins (β-CD) doped lipid-based vesicles as the core (receptacle) and β-CD modified camptothecin (CPT) pro-drug as the flyable dandelion seeds. The β-CD modified CPT pro-drug was introduced into the core vesicles in succession via host-guest interaction between inter-molecular β-CD and CPT, and cRGDfk peptides were further introduced as the outermost layer (stigma) to enhance the internalization into cancer cells . CPT interacted with β-CD through glutathione (GSH)-cleavable disulfide bonds, which led to drug release in glutathione-rich cancer cells, just as spread of dandelion seeds in the wind. GSH consumption further disrupted the intracellular redox homeostasis of cancer cells through combined action of Ce6 with light irradiation and the synergistic anti-tumor effect was thus achieved, resulting in apoptosis of cancer cells. Therefore, the nanomedicine provides a facile and versatile anti-tumor strategy, as well as a persistent anti-cancer effects.
研究领域:
纳米技术
·
肿瘤学
·
药学
·
化疗
·
光动力疗法
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2021
·
IF 14.593
Bailiang Wang,Liyang Zhou,Yishun Guo,Hanwen Guo,Yiming Zhong,Xiaomin Huang,Yifan Ge,Qingying Wang,Xiaoying Chu,Yingying Jin,Kaiyue Lan,Mei Yang,Jia Qu
Photodynamic therapy (PDT) is an important technique to deal with drug-resistant bacterial infections in the post-antibiotic era. However, the hypoxic environment in intractable infections such as refractory keratitis and periodontitis, makes PDT more difficult. In this work, spontaneous oxygen-producing cyanobacteria were used as the carrier of photosensitizer (Ce6), and ultrasmall Cu 5.4 O nanoparticles (Cu 5.4 O USNPs) with catalase activity for infection and inflammation elimination and rapid tissue repair (CeCycn-Cu 5.4 O). The loading of Ce6 and Cu 5.4 O USNPs onto cyanobacteria surface were confirmed by transmission electron microscopy, nano particle size analyzer, scanning electron microscopy. In vitro sterilization and biofilm removal experiments demonstrated that the restriction of hypoxic environment to PDT was significantly alleviated due to the oxygen production of cyanobacteria. Under laser irradiation, the close transfer of energy photons to oxygen produced by cyanobacteria reduced more than 90% of Ce6 dosages (660 nm, 200 mW/cm 2 , 2 min). It is worth mentioning that both rapid sterilization through PDT and long-term oxidized free radicals elimination were achieved by adjusting the ratio of Ce6 and Cu 5.4 O USNPs. Both periodontitis and refractory keratitis animal models proved the excellent self-oxygenation enhanced antibacterial property and promotion of tissue repair.
研究领域:
生物医学工程
·
微生物学
·
药学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
·
2022
·Vol. 49
·期 8
·2655-2667
·
IF 10.057
Hou Zhenyu,Zhou Mei,Ma Yuanyuan,Xu Xiaoxuan,Zhang Zhiqi,Lai Shiwei,Fan Wenpei,Xie Jinbing,Ju Shenghong
Purpose Radiation therapy (RT) and photodynamic therapy (PDT) are promising while challenging in treating tumors. The potential radiation resistance of tumor cells and side effects to healthy tissues restrict their clinical treatment efficacy. Effective delivery of therapeutic agents to the deep tumor tissues would be available for tumor-accurate therapy and promising for the tumor therapy. Thus, developing nanoprobes with effectively delivering radiotherapy sensitizers and photosensitizers to the interior of tumors is needed for the accurate combined RT and PDT of tumor. Methods The size-changeable nanoprobes of Gd 2 O 3 @BSA-BSA-Ce6 (BGBC) were synthesized with a crosslinking method. Magnetic resonance imaging (MRI) and in vivo near-infrared (NIR) imaging were measured to evaluate the nanoprobes’ tumor accumulation and intratumor penetration effect. The tumor suppression effect of combined RT and PDT with these nanoprobes was also studied for the 4T1 bearing Balb/c mice. Results The nanoprobes BGBC showed high tumor accumulation and disintegrated into small particles responding to the photo-irradiation-produced reactive oxygen species (ROS), allowing for tumor penetration. Abundant radiotherapy sensitizers and photosensitizers were delivered to the deep tumor tissues, which is available for the accurate therapy of tumor. In addition, the BGBC displayed outstanding MRI and fluorescence imaging effects for evaluating the biodistribution and tumor suppression effect of nanoprobes. Consequently, significant tumor suppression effect was obtained based on the accurate tumor treatment with the combined RT and PDT. Conclusion The designed size-changeable nanoprobes BGBC showed excellent tumor accumulation and deep tumor penetration, resulting in a significant tumor suppression effect based on the combined RT and PDT. This study provides a novel strategy for dual delivery of radiotherapy sensitizers and photosensitizers into the deep tumor tissues and is promising for the accurate theranostics of tumor.
研究领域:
癌症治疗
·
放射医学
·
纳米医学
·
医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2021
·
IF 6.53
Mengdi Shang,Yiyang Wu,Yeyang Wang,Yanfei Cai,Jian Jin,Zhaoqi Yang
Triple negative breast cancer (TNBC) is a greatly aggressive subtype of breast cancer with high recurrence and mortality rates. Chemotherapy as a primary treatment for cancer is limited due to toxic side effects and drug resistance. Therefore, low toxicity and more effective breast cancer therapeutic approaches are greatly desired. In this study, a strategy which using ZIF-90 nanoparticles co-deliver Ce6-anti-miR-21 and Ce6-anti-miR-155 into the tumor cells was developed. Due to the pH responsive drug release of ZIF-90, antisense oligonucleotides (anti-miRNAs) and photosensitizers are able to be efficiently released inside tumor microenvironment. The nano delivery system captures overexpressed oncogenic miRNAs while the photosensitizer Ce6 generates ROS under light irradiation to effectively induce the apoptosis of tumor cell. This combinatorial effect was verified by results showing that the purposed therapic method could effectively inhibit tumor cell proliferation and metastasis. The concept of antisense oligonucleotide combined with photodynamic therapy has great potential in cancer treatment or adjuvant therapy.
研究领域:
肿瘤学
·
分子生物学
·
纳米技术
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）