ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 10
·26387-26396
·
IF 5.19
Cao Zhihan,Li Ping,Ru Jinchuang,Cao Xuqian,Wang Xu,Liu Bin,Li Zhi-Hua
Triphenyltin (TPT) is a herbicide and antifouling agent that has been widely used. After TPT flows into water bodies, it will cause toxic effects on marine life. We evaluated the effect of environmental concentration level (0, 10, 100, and 200 ng/L) on the cell density, antioxidant capability, and photosynthesis-related genes in the marine Chlorella sp. The results showed that 10 and 100 ng/L TPT can promote the growth of marine Chlorella sp., 200 ng/L TPT can inhibit the growth of marine Chlorella sp., and the TPT toxicity was accumulative. The chlorophyll composition changed. The content of chlorophyll a in 100 ng/L and 200 ng/L groups was significantly higher than that in the control group ( p < 0.05) in 13 days. The content of chlorophyll b in the 100 ng/L and 200 ng/L groups in 1 day and 13 days was significantly different from that in the control group ( p < 0.05). The content of total chlorophyll in the 100 ng/L and 200 ng/L groups in 13 days was higher than that in the control group ( p < 0.05). The 200 ng/L group began to suffer oxidative damage on the 12th day, and the pigment protein complex responded to oxidative damage through self-feedback regulation. On the 18th day, chld , cao , psy , rbcS , and rbcL genes were downregulated, and psbA gene was upregulated in the 10 ng/L and 100 ng/L groups, which may be a feedback regulation of self-oxidative damage. This paper analyzed toxicity of environmental levels of TPT to marine Chlorella sp., which provided new data support for the comprehensive evaluation of its marine ecological toxicity.
研究领域:
海洋生物学
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环境毒理学
·
藻类学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Kai Liu,Jiping Li,Yuhao Zhou,Wei Li,Hu Cheng,Jiangang Han
The ecological effects of antibiotics in surface water have attracted increasing research attention. In this study, we investigated the combined ecotoxicity of erythromycin (ERY) and roxithromycin (ROX) on the microalgae, Chlorella pyrenoidosa , and the removal of ERY and ROX during the exposure. The calculated 96-h median effect concentration (EC 50 ) values of ERY, ROX, and their mixture (2:1 w/w) were 7.37, 3.54, and 7.91 mg∙L -1 , respectively. However, the predicted EC 50 values of ERY+ROX mixture were 5.42 and 1.51 mg∙L -1 , based on the concentration addition and independent action models, respectively. This demonstrated the combined toxicity of ERY+ ROX mixture showed an antagonistic effect on Chlorella pyrenoidosa . During the 14-d culture, low-concentration (EC 10 ) treatments with ERY, ROX, and their mixture caused the growth inhibition rate to decrease during the first 12 d and increase slightly at 14 d. In contrast, high-concentration (EC 50 ) treatments significantly inhibited microalgae growth ( p < 0.05). Changes in the total chlorophyll contents, SOD and CAT activities, and MDA contents of microalgae suggested that individual treatments with ERY and ROX induced higher oxidative stress than combined treatments. After the 14-d culture time, residual Ery in low and high concentration Ery treatments were 17.75% and 74.43%, and the residual Rox were 76.54% and 87.99%, but the residuals were 8.03% and 73.53% in ERY+ ROX combined treatment. These indicated that antibiotic removal efficiency was higher in combined treatments than that in individual treatments, especially at low concentrations (EC 10 ). Correlation analysis suggested that there was a significant negative correlation between the antibiotic removal efficiency of C. pyrenoidosa and their SOD activity and MDA content, and the enhanced antibiotic removal ability of microalgae benefited from increased cell growth and chlorophyll content. Findings in this study contribute to predicting ecological risk of coexisting antibiotics in aquatic environment, and to improving biological treatment technology of antibiotics in wastewater.
研究领域:
环境科学
·
生物学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Zezheng Wang,Dongdong Fu,Liu Gao,Huaiyuan Qi,Yuanyuan Su,Licheng Peng
Environmental aging of ubiquitous microplastics (MP) occurs through the action of biotic and abiotic factors, and aged MP exhibit different physicochemical properties and environmental behavior from virgin MP. This study aimed to investigate the aged micro-sized polystyrene (mPS) and polyvinyl chloride (mPVC), and the heavy metals copper (Cu) and cadmium (Cd), and examine the effects of their combined toxicities on microalga Chlorella vulgaris . Results showed that the presence of MP inhibited cell growth as compared with the control, the inhibition rate ( I ) decreased as concentrations of MP rose and aged MP exhibited stronger inhibition of cells than did virgin MP. The largest I was achieved in each culture with the MP concentration of 0.01 g/L, in which aged mPS with the maximal of 36.84% ( I aged mPS ) followed by aged mPVC ( I aged mPVC = 30.03%), virgin mPS ( I virgin mPS = 29.10%) and virgin mPVC ( I virgin mPVC = 16.72%). Addition of the heavy metals Cu 2+ and Cd 2+ significantly inhibited cell growth, and toxicity increased with concentrations in a range of 0.5–2.0 mg/L; the maximum I values were 19.50% ( I Cu ) and 85.14% ( I Cd ), respectively. The combined toxicity of aged MP + Cu or aged MP + Cd was less than that of individual heavy metals. In particular, as compared with the maximal I Cd of 85.14% achieved by single Cd 2+ , the toxicity of Cd 2+ was greatly reduced when combined with aged mPS and mPVC, with the I value decreased to 27.55% ( I aged mPS ) and 32.51% ( I aged mPVC ), respectively. Both single and combined treatments caused cell damage to the microalga, accompanied by increased superoxide dismutase (SOD) and intracellular malonaldehyde (MDA) content.
研究领域:
环境科学
·
毒理学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2024
·
IF 4.6
Yakoub Ladjal-Ettoumi,Meriem Hamadi,Lina Hadjer Douik,Zakaria Cherifi,Akmal Nazir
This comparative study investigates the physicochemical, functional, and nutraceutical properties of Spirulina ( Arthrospira platensis ) and Chlorella sp. biomass, two of the most prevalent microalgae species. We assessed their proximate composition, pH, acidity, color, granulometry, microstructure (SEM), functional groups (FTIR), crystallinity (XRD), and thermal stability (GTA). Functional properties were thoroughly examined, including bulk density, water and oil holding capacities, and gelling, foaming, and emulsifying capabilities. Additionally, the study quantified the content of pigments, phenolic compounds , carotenoids , and flavonoids and evaluated antioxidant activity . Our findings indicate that Spirulina biomass exhibits higher protein and ash content, whereas Chlorella biomass surpasses phenolic compounds, carotenoids, and antioxidant activity. Notable differences were observed in their microstructure and thermal degradation patterns. Both biomasses demonstrated considerable potential as functional ingredients, owing to their superior water and oil retention, foaming, emulsifying, and gelling properties. This investigation underscores the distinct physicochemical and nutraceutical profiles of Spirulina and Chlorella biomass, highlighting their significant applicability in the food industry for developing novel value-added food products.
研究领域:
食品科学
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生物技术
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DOI
高影响因子
Water
·
2021
·Vol. 13
·期 18
·2498
·
IF 3.103
Wenlong Lu,Yifeng Xu,Chuanzhou Liang,Baba Imoro Musah,Lai Peng
The biosorption behaviour of arsenic(V) and cadmium(II) ions by unmodified and five types of chemically modifiedChlorella vulgarisandSpirulina platensiswas investigated. The biosorption rates of As(V) and Cd(II) in binary metal solutions were lower than those in sole metal systems, which exhibited a competition between As(V) and Cd(II) ions to occupy the active sites of the adsorbent. Among the five chemical reagents, NaCl and ZnCl2were the most suitable modifiers for improving the biosorption performance ofC. vulgarisandS. platensis, respectively. The maximum biosorption capacities of As(V) and Cd(II) were: (a) 20.9 and 1.2 mg/g, respectively, forC. vulgarismodified with NaCl; (b) 24.8 and 29.4 mg/g, respectively, forS. platensismodified with ZnCl2, which were much higher than those using other chemically modifying methods. The pseudo-second-order kinetic model fitted well with all the biosorption processes. The SEM analysis revealed that the modification changed the surface morphologies and enhanced the porosity of the algae biomass. The FTIR analysis established the presence of diverse groups of compounds that were largely hydroxyl, carboxylate, amino, and amide groups on the adsorbents that contributed significantly to the upregulated biosorption. This work showed the potential application of chemically modifiedC. vulgarisandS. platensisbiomasses to effectively remove both from water.Keywords:arsenic;cadmium;simultaneous biosorption;algae;chemical modification
研究领域:
环境科学
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生物学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Zhaochuan Li,Xianliang Yi,Hao Zhou,Tongtong Chi,Wentao Li,Kaiming Yang
The combined effect of polystyrene microplastics (mPS) and dibutyl phthalate (DBP), a common plastic additive, on the microalgae Chlorella pyrenoidosa was investigated in the present study. The 96 h-IC 50 value of DBP was 2.41 mg L −1 . Polystyrene microplastics exhibited size-dependent inhibitory effect to C. pyrenoidosa , with the 96 h-IC 50 at 6.90 and 7.19 mg L −1 for 0.1 and 0.55 μm mPS respectively, but little toxicity was observed for 5 μm mPS. The interaction parameter ρ based on the response additive response surface (RARS) model varied from −0.309 to 5.845, indicating the interaction pattern varying with exposure concentrations of chemical mixtures. A modified RARS model (taking ρ as a function of exposure concentration) was constructed and could well predict the combined toxicity of mPS and DBP. More than 20% reduction of DBP was observed at 20 mg L −1 mPS, while 1 mg L −1 mPS had no significant effect on the bioavailability of DBP at different sampling time points. Volume, morphological complexity and chlorophyll fluorescence intensity of microalgal cells were disturbed by both DBP and mPS. The antagonistic effect of high concentrations of mPS might be partially attributed to the combination of hetero- and homo-aggregation and the reduced bioavailability of DBP. The overall findings of the present study profiled the combined toxic effects of mPS and DBP on marine phytoplankton species which will be helpful for further evaluation of ecological risks of mPS and DBP in marine environment.
研究领域:
环境科学
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毒理学
·
海洋生物学
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DOI
PubMed
高影响因子
Food Biophysics
·
2024
·Vol. 20
·期 1
·1-16
·
IF 2.8
Yu Cailing,Zhao Yanan,Zu Xinyu,Liang Yan,Wang Hua
This study presents a novel multifunctional hydrogel synthesized by crosslinking Chlorella protein using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/n-hydroxysuccinimide, aimed at extending the shelf life of perishable fruits like cherries. Structural, rheological, and scanning electron microscopy analyses revealed that Chlorella protein hydrogels (CPH) possess excellent solid-like properties and a stable porous structure. The water-holding capacity improved significantly from 67.11 ± 0.72% to 96.53 ± 0.61% with increasing CP concentration (10–22.5%, w/v). Additionally, CPH decomposition temperatures were ~ 150 °C (5% weight loss), demonstrating good thermal stability. Due to the ionization of -COOH and -NH 2 groups, the CPH showed excellent pH sensitivity, with low dissolution rates in acidic environments (64.97%) and significantly higher rates in alkaline environments (448.50%). Furthermore, the CPH inhibited the penetration of Staphylococcus aureus and Escherichia coli , and exhibited good free radical scavenging abilities against DPPH (74.50%) and ABTS •+ (97.92%). In cherries preservation tests, CPH extended preservation time to 15 days compared to 5 days in the control group, effectively inhibiting decay, suggesting CPH is a promising choice for multifunctional fruit preservation. Graphical abstract
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Zeliang Liu,Xuanlin Cao,Manli Wu,Wenjie Huang,Xia Dong,Xing Chen,Chun Zhang
Perfluorobutanoic acid (PFBA), an emerging alternative to perfluorooctanoic acid (PFOA), has become increasingly prevalent in aquatic ecosystems, yet its ecotoxicological impacts remain poorly understood. This study investigated the aquatic toxicity of PFBA using the freshwater algae Chlorella vulgaris ( C . vulgaris ) as a model organism, employing a 96h pre-exposure assay to determine the median effective concentration followed by acute toxicity experiments analyzing multiple endpoints including growth, photosynthetic parameters, oxidative stress markers, and antioxidant enzyme activities. Computer simulation techniques were utilized to illustrate the underlying molecular mechanisms of PFBA toxicity. The results showed that the 96h-EC 50 value of PFBA was 154.88 mg/L, which is comparable to conventional per- and polyfluoroalkyl substances (PFAS). Acute toxicity experiments revealed a biphasic dose-response relationship to the algal growth with the hormetic effects at the lower concentrations (30.97–92.93 mg/L) but inhibition at the higher levels (123.91–185.86 mg/L) of PFBA. High dosages of PFBA significantly decreased the maximum photosynthetic yield (Fv/Fm) and relative electron transfer rate (rETR), while inducing oxidative stress and inhibiting superoxide dismutase (SOD) and catalase (CAT) activities. Future AlphaFold2 modeling and molecular docking simulations demonstrated the potential binding of PFBA to photosystem II D1 C-terminal processing protease (PSII D1 protein), SOD, and CAT. These findings reveal a complex toxicity mechanism of PFBA on C . vulgaris involving photosynthetic disruption, oxidative stress, and antioxidant system impairment, contributing to the understanding of short-chain PFAS alternative ecotoxicity in aquatic ecosystems.
研究领域:
环境科学
·
毒理学
·
分子生物学
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DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 5
·647
·
IF 4.7
Lin Qi,Teng Hang,Weijie Jiang,Sinong Li,Hui Zhang,Xiang Liang,Le Lei,Qiangqiang Bi,Hang Jiang,Yunxing Li
Microalgae are highly regarded as ideal materials for the creation of liquid biofuels and have substantial potential for growth and utilization. However, traditional storage and culture methods for microalgae are plagued by challenges such as uncontrolled growth, bacterial contamination, and self-shading among algae. These issues severely impede the photosynthetic process and the efficient extraction of biomass energy. This study tackles these problems by utilizing magnetic hydrophobic protein particles to stabilize water-in-oil Pickering emulsions. This allows for the micro-compartment storage and magnetic transfer of algae. Additionally, the successful encapsulation of Chlorella cells in high-internal-phase water-in-oil Pickering emulsions effectively mitigates the settling problem of Chlorella cells in the liquid phase, thereby enabling the potential use of Pickering emulsions for the confined cultivation of microalgae.
研究领域:
生物技术
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Wenjie Yuan,Yicheng Wu,Yeling Zhou,Bingjun Shu,Jinqi Cai,Haiyan Fu
Traditional wastewater treatment processes have a relatively low removal rate of the tricyclic antidepressant amitriptyline. This study employed Chlorella vulgaris as the test organism to investigate the effects of exposure to different concentrations of amitriptyline on various biological functions of the microalga. The results showed that the physiological activities of C. vulgaris were inhibited by amitriptyline primarily through the disruption of photosynthesis, the imbalance of the antioxidant system, and the suppression of photosynthesis-related gene expression. The content of chlorophyll a was significantly reduced by 72.0 %, while the activities of superoxide dismutase and catalase were increased to 217.5 % and 67.2 %, respectively. The transcriptional levels of PsaB and PsbC genes were significantly suppressed by 75.5 % and 57.2 %, respectively. After 8 days of exposure to 5 mg/L amitriptyline, the maximum removal rate of amitriptyline by C. vulgaris was determined to be 62.62 %, with biodegradation identified as the predominant removal pathway. The removal of amitriptyline followed pseudo-first-order kinetics, with a degradation half-life of 5.93 days. The study provides theoretical foundations for optimizing microalgal based treatment processes of amitriptyline contaminated wastewater and delivers critical data support for environmental risk assessment of amitriptyline.
研究领域:
环境毒理学
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藻类学
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生物修复
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水生生态学
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药物污染
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产品关联
DOI
高影响因子
Water
·
2025
·Vol. 17
·期 21
·3140
·
IF 3
Yang Guo,Tao Zhu,Kangle Shao,Junhao Wang,Chengyu Zhou,Yingang Xue,Junhong Li
Tetracycline (TC), commonly utilized in medicine and aquaculture, frequently enters aquatic environments, raising ecological concerns. This study examined TC-contaminated wastewater treated through ultraviolet (UV), potassium persulfate (PS), and combined UV/PS disinfection processes. The degradation of TC followed pseudo-first-order kinetics, with removal efficiency ranked as UV/PS > UV > PS. High-performance liquid chromatography–mass spectrometry (HPLC-MS) identified 20 disinfection byproducts (DBPs) across all processes. Based on the identified intermediates, the degradation pathways of TC under different disinfection processes (UV, PS, and UV/PS) were elucidated. Using the ECOSAR program, both acute and chronic aquatic toxicities of TC and its DBPs were predicted. The biological effects onChlorellawere also investigated. DBPs from UV and PS treatments inhibited algal growth, reducing it by 4.8–9.4% relative to the control. Conversely, DBPs formed under UV/PS disinfection stimulated growth, increasing rates by 3.4–6.6%. To counteract oxidative stress from TC and its DBPs,Chlorellaenhanced superoxide dismutase (SOD) and catalase (CAT) activities. These findings highlight that while TC degradation occurs efficiently, the nature of DBPs and their ecological impacts vary significantly depending on the disinfection method. Overall, the UV/PS process not only improved TC removal but also reduced harmful effects on microalgal growth compared with UV or PS alone.
研究领域:
环境科学
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水处理技术
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生态毒理学
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光催化降解
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2025
·
IF 3.6
Dong-Ao Li,Guang-Hui Xu,Jie Shen,Hong Zhang
This study explores the mechanisms of oxidative damage in Chlorella sorokiniana under continuous gas-liquid phase plasma exposure. The oxidative damage mechanism of C. sorokiniana was revealed through multiple analytical techniques, including scanning electron microscopy (SEM), flow cytometry (FCM), and fluorescence excitation-emission matrix (EEM). Plasma-induced damage to algal cell membranes, causing seepage of photosynthetic pigments and dissolved organic matter; chlorophyll fluorescence changes confirmed damage to the photosynthetic system. Alterations in MDA, intracellular ROS/NO, and antioxidant enzyme activities reflect lipid peroxidation and oxidative stress levels. These results show that plasma induces oxidative damage to algal membranes, intracellular components, and photosystems via reactive oxygen and nitrogen species (RONS). C. sorokiniana activates its antioxidant defense system under stress. This gradual, cumulative damage exerts distinct biological effects at different times. The findings support the precise control of low-temperature plasma for beneficial microorganisms and provide a basis for advancing microalgae transformation technologies.
研究领域:
环境微生物学
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等离子体生物学
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氧化应激
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微藻生理学
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光合作用
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分子生态毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）