Journal of Materials Chemistry B
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2019
·Vol. 8
·期 5
·919-927
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IF 5.047
Minghui Zan,Cong Li,Daoming Zhu,Lang Rao,Qian-Fang Meng,Bei Chen,Wei Xie,Xingwang Qie,Li Li,Xiaojiao Zeng,Yirong Li,Wen-fei Dong,Wei Liu
Copper ions (Cu2+) and L-cysteine (L-Cys) in the human body always play critical roles in various physiological processes, while abnormal Cu2+ and L-Cys concentrations in the biological system lead to many diseases. In this manuscript, Si-doped carbon dots (Si-CDs) with near-infrared fluorescence were designed for the detection of Cu2+ and L-Cys through the fluorescence “on–off–on” mode. The carbon dots exhibited not only excellent optical merits including good stability against photobleaching and high chemical stability, but also superior biological compatibility. Interestingly, due to the abundant amino groups distributed on the surface of Si-CDs, they could bind to copper ions to form cupric amine complexes and then quench the fluorescence of Si-CDs due to an electron transfer process. In addition, upon the addition of L-Cys, the FL intensity of Si-CDs could be effectively recovered accompanied with complexation between Cu2+ and the functional groups in L-Cys, due to which Cu2+ was removed from the surface of Si-CDs. Notably, as far as we know, these are the first red-emitting carbon dots for copper ion and L-Cys assays in water samples and human plasma samples. Furthermore, this strategy was successfully applied to the determination of Cu2+ and L-Cys in living systems, demonstrating great practicability in biomedical applications.
研究领域:
分析化学
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生物医学工程
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材料科学
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Biao Zhang,Jun Cao,Si-Jie Liao,Peng-Cheng Zhou,Yu-Ting Shen,Wei Yu,Wei Li,Ai-Guo Shen
At present, there is a lack of sufficiently specific laboratory diagnostic indicators for schizophrenia. Serum homocysteine (Hcy) levels have been found to be related to schizophrenia. Cysteine (Cys) is a demethylation product in the metabolism of Hcy, and they always coexist with highly similar structures in vivo. There are few reports on the use of Cys as a diagnostic biomarker for schizophrenia in collaboration with Hcy, mainly because the rapid, economical, accurate, and high-throughput simultaneous detection of Cys and Hcy in serum is highly challenging. Herein, a click reaction-based surface-enhanced Raman spectroscopy (SERS) sensor was developed for simultaneous and selective detection of Cys and Hcy. Through the efficient and specific CBT-Cys click reaction between the probe containing cyan benzothiazole and Cys/Hcy, the tiny methylene difference between the molecular structures of Cys and Hcy was converted into the difference between the ring skeletons of the corresponding products that could be identified by plasmonic silver nanoparticle enhanced molecular fingerprint spectroscopy to realize discriminative detection. Furthermore, the SERS sensor was successfully applied to the detection in related patient serum samples, and it was found that the combined analysis of Cys and Hcy can improve the diagnostic accuracy of schizophrenia compared to a single indicator.
研究领域:
医学诊断
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分析化学
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生物医学工程
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Lili Lian,Xinyang Zhang,Jie Hao,Jinyi Lv,Xiyue Wang,Bo Zhu,Dawei Lou
In this study, a unique core-shell titanium-based metal-organic framework (MOF) functionalized magnetic microsphere was constructed and utilized as magnetic adsorbent for enrichment of five fluoroquinolones from water samples. The received magnetic MOF nanocomposite, denoted as Fe 3 O 4 @Cys@MIL125-NH 2 , showed strong magnetic responsiveness, and good hydrophilicity, as well as excellent affinity to the target analytes. Under optimal conditions, the developed magnetic solid-phase extraction (MSPE) method, combined with ultra-high performance liquid chromatography (UPLC), showed good linearity within the studied concentration range, and low limits of detection (0.05-0.2 μg L −1 ). The accuracy of the developed method was evaluated by measuring the extraction recovery of the spiked tap water and river water samples. The satisfactory recoveries of five fluoroquinolones were in the range of 83.8–109.4% with the RSDs lower than 8.9%. These results indicated that the as-prepared Fe 3 O 4 -Cys@MIL125-NH 2 is a promising magnetic adsorbent for efficient enrichment of fluoroquinolones from tap water and environmental water samples.
研究领域:
环境科学
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分析化学
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水处理
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PubMed
高影响因子
ELECTROCHIMICA ACTA
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2026
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IF 5.8
Mei Wang,Lei Yang,Zesheng Wang,Liying Jiang
A novel N-MLG@Ni–Mn LDH/Cys electrode was constructed for Cu²⁺ detection in sweat. • Dual active sites significantly enhance Cu²⁺ adsorption and signal amplification. • The sensor exhibits a wide detection range and a low detection limit for Cu²⁺. • The synergistic effect of N-MLG and Ni–Mn LDH improves electrochemical performance. In this study, a highly sensitive electrochemical sensor based on a N-MLG@Ni-Mn LDH/Cys nanocomposite was fabricated for the detection of trace Cu²⁺ in sweat. Nitrogen-doped multilayer graphene (N-MLG) was electrostatically self-assembled with nickel-manganese layered double hydroxide (Ni-Mn LDH) via their charge complementarity, forming a uniform heterostructure. L -cysteine (Cys) was further introduced as a surface modifier to construct the N-MLG@Ni-Mn LDH/Cys nanocomposite-based electrochemical sensor. Based on the hard-soft acid-base (HSAB) theory, a synergistic adsorption mechanism involving dual active sites was established, which significantly enhanced the preconcentration efficiency and specific recognition ability toward copper ions. Electrochemical measurements were performed using square-wave anodic stripping voltammetry (SWASV). Electrochemical characterization demonstrated that the sensor possesses excellent conductivity and good electrochemical activity. Under optimized experimental conditions, the sensor exhibited outstanding analytical performance. A good linear relationship was obtained for Cu²⁺ in the concentration range of 0.1–500 ppb, with a low detection limit of 0.24 ppb. In addition, the sensor prepared by this method showed good anti-interference capability, repeatability, and long-term stability, and it also performed well in the detection of artificial sweat samples. Overall, these performance advantages provide a feasible technical strategy for noninvasive monitoring of Cu²⁺ in sweat, with broad application prospects. An electrochemical sensing platform was constructed for the sensitive detection of Cu²⁺ in sweat based on an N-MLG@Ni-Mn LDH/Cys composite-modified screen-printed electrode (SPE). The electrostatic self-assembly between negatively charged N-MLG nanosheets and positively charged Ni-Mn LDH laminates leads to a stable intercalated heterostructure with high conductivity and abundant active sites. Subsequent functionalization with L-cysteine introduces sulfhydryl (-SH) groups, enabling specific chelation toward Cu²⁺ and enhancing selectivity. During detection, Cu²⁺ is effectively preconcentrated via the synergistic interactions of hydroxyl (-OH) and sulfhydryl (-SH) groups, followed by electrochemical reduction to Cu⁰ under a negative potential. The deposited Cu⁰ is then oxidized during the stripping process, generating a characteristic current signal proportional to the Cu²⁺ concentration. Combined with SWASV, this dual-active-site strategy achieves high sensitivity, excellent selectivity, and reliable analytical performance, demonstrating its effectiveness for trace Cu²⁺ detection in sweat. Download: Download high-res image (221KB) Download: Download full-size image
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高影响因子
Nanoscale Advances
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2023
·Vol. 5
·期 18
·5029-5035
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IF 4.7
Haoyu Wang,Xiaomeng Yue,Huanhuan Wu,Yeda Wan,Yujie Tong,Yang Zhao,Yijun Li,Jinbin Pan
Nanomaterial-mediated photothermal therapy (PTT) is a promising strategy for permanent male sterilization owing to its easy operation, rapid heating, minimal invasiveness, and high spatiotemporal controllability. However, the currently available PTT for male sterilization utilizes irradiation sources in the first near-infrared window (NIR-I), which may suffer from incomplete sterilization due to the insufficient penetration depth of NIR-I light. Herein, we developed a facile one-pot hydrothermal synthetic method of cysteine-coated copper sulfide (Cys-CuS) nanosheets for the second NIR window (NIR-II) PTT-mediated permanent male sterilization. In this method, Cys acted not only as a template but also as a sulfur resource in the formation of Cys-CuS nanosheets. The obtained Cys-CuS nanosheets possessed good photothermal properties and satisfied deep-tissue light response capacity under 1064 nm laser exposure. Given this, the permanent male sterilization in vivo was readily achieved by Cys-CuS nanosheets in a rapid manner (only 40 s). To the best of our knowledge, it is the first time that nanomaterial-mediated NIR-II PTT is applied for permanent male sterilization. We believe that the facilely prepared biocompatible Cys-CuS nanosheets can serve as a promising NIR-II light-responsive nanoknife to control the overpopulation of domestic pets and stray animals.
研究领域:
生物医学工程
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材料科学
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光热疗法
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生殖医学
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高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 8
·827
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IF 5.4
Meixuan Liu,Chen Yan,Qianyun Ye,Xiaohuan Sun,Jie Han
Glutathione (GSH) is the most abundant low-molecular-weight biological thiol in vivo and has been linked to several diseases. The accurate quantification of GSH is therefore crucial for disease diagnosis and monitoring. In this study, we prepared self-assembled Cu(I)-Cys (cysteine) nanozymes through a two-step procedure. The Cu(I)-Cys nanoparticles exhibited peroxidase-mimicking activity. Upon the addition of H2O2, they were able to oxidize 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB, resulting in a measurable increase in UV-Vis absorption at 655 nm. However, in the presence of GSH, oxTMB was reduced back to TMB, leading to a decrease in UV-Vis absorption at 655 nm. By utilizing these changes in the absorption intensity, we achieved the sensitive detection of GSH with a detection limit of 2.13 μM. Moreover, taking advantage of the different peroxidase-mimicking activities of Cu(I)-Cys nanoparticles at various pH values, a sensor array with Cu(I)-Cys nanoparticles at pH 4 and pH 5 was constructed. The discrimination of GSH among Cys and ascorbic acid was achieved and the practicability of the sensor array in human serum was validated. This novel approach holds significant promise for the precise discrimination and quantification of GSH and its potential applications in disease diagnosis and therapeutics.Keywords:nanozyme;GSH quantification;GSH discrimination
研究领域:
生物化学
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分析化学
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纳米技术
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诊断学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Xiaojie Ren,Huihui Tian,Liu Yang,Long He,Yani Geng,Xingjiang Liu,Xiangzhi Song
Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are of great importance in many physiological and pathological processes. Due to their similar structure and reactivity of these three biothiols, it is a great challenge to simultaneously distinguish one from another. By virtue of two different fluorescence signals, nitrobenzofurazan ( NBD ) has been proved to be an effective sensing functional group as well as a green fluorophore precursor to construct fluorescent probes for simultaneous discrimination of Cys/Hcy and GSH. However, most of the reported NBD-based fluorescent probes suffered from a poor water-solubility, a slow response, double-wavelength excitation or two interfering fluorescence signals (narrow wavelength separation in λ max Em and/or big difference in Φ Fl ). In this study, we developed a fluorescent probe, MCQ-O-NBD , for simultaneous discrimination of Cys/Hcy and GSH using two matched fluorescent signals (well separated red and green fluorescence and comparable Φ Fl ), which could function in a pure aqueous media with a rapid response under a single-wavelength excitation. This probe exhibited high sensitivity and good selectivity as well as low detection limit, and had been successfully applied to visualize and differentiate Cys/Hcy and GSH in living cells.
研究领域:
生物化学
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医学检测
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荧光成像
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2026
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IF 4
Gege Zhu,Li Qi,Bing Niu,Qi Zhang,Qin Chen,Dan Zhong,Xiaodong Sun
Metal-organic frameworks (MOFs) are promising chiral separation materials owing to their well-defined pore structures and tunable functionality. In this work, mesoporous/microporous NU-1000 was functionalized with L-Cysteine (L-Cys) via solvent-assisted ligand exchange to produce L-Cys-NU-1000, which was applied as a stationary phase in capillary electrochromatography (CEC). The resulting L-Cys-NU-1000@capillary system achieved baseline separation of five chiral amino acid enantiomers with excellent intra-day, inter-day, and batch reproducibility (RSDs < 5%). Notably, the system retained high enantioselectivity even after 100 consecutive injections. This study represents the first application of NU-1000 in chiral separation, opening new avenues for its use and laying a foundation for broader future applications. The system demonstrates distinct advantages for quality control of amino acids, offering high efficiency, robustness, and reproducible enantiomeric separation crucial for food and pharmaceutical analysis.
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高影响因子
JOURNAL OF LUMINESCENCE
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2020
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IF 3.28
Jin Bai,Xin Yang,Ying Qian
In this work, a unique ratiometric and NIR fluorescent probe , Hecy-NBD, was designed and synthesized to detect cysteine/homocysteine/glutathione in aqueous solution. Hemicyanine skeleton was chosen as the major fluorophore and nitro benzoxadiazole (NBD) chromophore was selected as the recognition unit to construct a ratiometric fluorescent probe. The fluorescence of probe Hecy-NBD was very weak due to the introduction of quenching group NBD . Upon the addition of 100 μM GSH to 10 μM Hecy-NBD, the fluorescence intensity at 702 nm was very strong with the releasing of compound Hecy-OH. While upon the addition of 100 μM Cys or 100 μM Hcy, probe exhibited distinct fluorescence enhancement at 540 nm and 702 nm, which resulted from the formation of compound NBD-Cys and NBD-Hcy through the 5/6-membered cyclic transition and the fluorescence releasing of compound Hecy-OH. The proposed mechanism based on S N Ar reaction and molecular rearrangement, which were confirmed by HRMS spectra. The detection limit of these three biothiols was also calculated (GSH, 50 nM; Cys, 81.0 nM; Hcy, 130 nM). Moreover, probe Hecy-NBD could function well at wide pH conditions from 4 to 10. Besides that, probe Hecy-NBD with low cell toxicity had been successfully applied to dual-channel fluorescence imaging in living cells. Considering the good characteristics, Hecy-NBD had great potential to detect biothiols-associated diseases in the future.
研究领域:
生物化学
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医学诊断
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分子生物学
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高影响因子
DYES AND PIGMENTS
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2023
·
IF 4.5
Xiumin Liu,Hengwei Zhang,Fengting Hu,Yi Wang,Xu Fei,Jing Tian
In this paper, an efficient and novel 1,3-bis(2-pyridylimino)-4,7-dihydroxyisoinndole scaffold-based two-site fluorescent probe 1,3-bis(2-pyridylimino)-4,7-phenylene diacrylate isoindole (BPI-AC) for the sensing of cysteine (Cys) has been designed and synthesized. We have studied the chemical structure and its fluorescent properties for the novel fluorescent probe with double recognition group by means of theoretical calculations and experimental measurements, which were carried out using density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations and experiments on organic chemical synthesis. Firstly, geometries have been recorded for the fluorescent probe. Theoretical research parameters including spectroscopic parameters, molecular orbitals , reaction sites and energy barrier Δ were obtained by fitting the electron density in a multiparticle system. Additionally, the spectral characteristics , selectivity and anti-interference performance of the probe were systematically investigated and verified by in vitro experiments. The experimental results showed that by using this probe, it was possible to quantitatively detect Cys in 1% dimethyl sulfoxide-containing water solution (pH 7.4). Super large Stokes shift (265 nm) endowed the probe low background signal. Finally, according to our theoretical calculations and the available experimental data, the probe BPI-AC with dual recognition sites could distinguish the biothiols with similar structure through series recognition reaction, and the Stokes shift was large, realizing the efficient detection of Cys specificity.
研究领域:
分析化学
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理论化学
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生物化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Qian Wang,Wenting Zhang,Mingzhe Cai,Yafei Ma,Ajuan Yu,Sheng Chen,Shusheng Zhang
The reactive sulfur species (RSS), in particular Cys, Hcy , GSH, and H 2 S, play an essential role in lots of critical physiological and pathological processes. To elucidate their complex interrelationships in signaling and metabolic pathways, suitable molecular tools to simultaneously distinguish Cys, Hcy , GSH, and H 2 S are urgently needed. Herein, we developed a novel cascade reaction-assisted trinal-site fluorescent probe BCS by connecting three functional fragments for simultaneously distinguishing Cys, Hcy , GSH, and H 2 S. The probe BCS exhibited red fluorescence in the absence of the four biological RSS. However, with the attendance of Cys, Hcy , GSH, and H 2 S, the probe showed different fluorescence patterns, including blue, blue/red, green/red, and no fluorescence, respectively. More importantly, based on the different RSS-induced fluorescence patterns and multi-color fluorescence imaging, probe BCS was used to discriminate and visualize all four biological RSS in both HepG2 cells and zebrafish. It is expected that probe BCS will have applications in the diagnosis and research of RSS-related diseases in the future.
研究领域:
生物化学
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分子生物学
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医学诊断
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-11
·
IF 5.3
Chen Wenxia,Hu Shuangyi,Zhou Bingqian,Ren Yueran,Xue Yangyang,Yang Rui
Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFe 2 O 4 @mSiO 2 -Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE. The material combines the advantages of magnetic separation, mesoporous loading, catalytic enhancement, and biorecognition. CuFe 2 O 4 NPs offer efficient magnetic separation, while the mesoporous silica (mSiO 2 ) layer is effectively loaded with luminol to amplify CL signals. L-cysteine (L-Cys) ensures the uniform distribution of luminol and gold nanoparticles (Luminol-Au NPs), with these Au NPs immobilizing Abs through Au–S and Au–N bonds, achieving high-density biocoupling. The constructed label-free CLIA enables highly sensitive detection of NSE without Abs labeling. The results show that the NSE concentration is linearly correlated with the CL intensity in the range 1.0 × 10 −13 ~ 1.0 × 10 −8 g/mL, and the detection limit is as low as 2.3 × 10 −14 g/mL. The proposed method demonstrates excellent selectivity, stability, and anti-interference capability for the detection of NSE in human serum samples. Graphical
研究领域:
生物医学工程
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分析化学
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纳米技术
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癌症诊断
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生物传感器
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高影响因子
危险品化学品经营许可证（带存储）