Biomass Conversion and Biorefinery
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2019
·Vol. 10
·期 2
·277-287
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IF 2.326
Shi Ning,Liu Qiying,Cen Hu,Ju Rongmei,He Xiong,Ma Longlong
Humins are undesired solids formed during the hydrothermal degradation of carbohydrates. In order to reveal the mechanism of formation of humins, we studied the degradation behavior of 11 model compounds including carbohydrates and furfural derivatives, within water and various pure organic solvents as reaction media. All the studied carbohydrates could generate solid humins in both water and studied organic solvents except ethanol, while the furfural derivatives could generate solid humins in only water. The results could be explained by regarding the formed α-carbonyl aldehydes and α,β-unsaturated aldehydes as primary precursors for formation of humins. Furfural derivatives could generate chain α-carbonyl aldehydes (for example, 6-hydroxy-2,5-dioxohexanal from 5-hydroxymethylfurfural and 2-oxopentanedial from furfural) through hydrolytic ring opening reaction; thus, water is essential for these furfural derivatives to generate humins. As for carbohydrates, they could generate α-carbonyl aldehydes and α,β-unsaturated aldehydes through simple step of β-elimination in all solvents; thus, they could form humins in both water and studied organic solvents except ethanol. Ethanol could react with α-carbonyl aldehydes by acetalization; thus, the condensation between α-carbonyl aldehydes was suppressed in ethanol, leading to few humins formation from carbohydrates. Based on the above analysis, we proposed that the formed α-carbonyl aldehydes and α,β-unsaturated aldehydes should be the primary precursor of humins.
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CHEMISTRY OF MATERIALS
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2019
·
IF 10.159
Jinrong Liu,Lei Xie,Jiang Deng,Yutong Gong,Guping Tang,Hongzhen Bai,Yong Wang
Here, hydrothermal biomass-derived nanospheres were designed to enhance cellular delivery in biomedicine and to overcome the difficulties in the synthesis of small mesoporous hydrothermal carbonaceous nanospheres (SMHNs) below 100 nm in size. A facile kinetics-controlled soft-template strategy was developed to construct annular SMHN with precisely tunable sizes of 30–80 nm, ordered channels, and abundant functional glycosylation groups using monosaccharides as the carbon source. SMHN exhibited a high loading capacity of 503 mg g–1 for doxorubicin due to the mesoporous structure and rich functional glycosylation groups. Moreover, SMHN displayed low cytotoxicity and few hemolytic effects and biological interactions, showing efficient internalization performance. Cells treated with fluorescein isothiocyanate-loaded SMHN had high mean fluorescence intensity within 75 min, which is significantly higher than that of the carbon counterparts. It is believed that the biological interactions of SMHNs are attributed to the reserved functional glycosylation groups that can activate glucose transporters and mediate endocytosis across the cytomembrane. The approach used to construct SMHN provides great opportunities for the generation of small mesoporous carbonaceous particles with rough annular structures. Moreover, the bottom-up construction strategy provides new understanding of the rational design and synthesis of biomass-derived vectors for efficient drug delivery.
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FOOD CHEMISTRY
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2018
·
IF 4.946
Linglin Fu,Chong Wang,Jinbao Wang,Saiqiao Ni,Yanbo Wang
Tropomyosin (TM), the predominant shellfish allergen, has induced severe food allergy worldwide, but efficient prevention and cure measures are limited nowadays. In the present study, we introduced an approach to eliminate Penaeus chinensis TM by Maillard reaction with reducing sugars including ribose, galacto-oligosaccharide (GOS) and chitosan-oligosaccharide (COS), which reduced the allergenicity by up to 60%. We also evaluated the conformational changes of TM during reaction by far-UV circular dichroism, and found the reaction-induced alternation from α-helix to β-sheet. We further determined the effect of Maillard reaction on TM allergenic epitopes to investigate the detailed mechanisms, and revealed the structure preference of different sugars. Correlation analysis with experimental data revealed that α-helix mediated the allergenicity elimination by Maillard reaction. There results provided theoretical foundations for the improvement and application of Maillard reaction in allergenic food processing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Qingxia Yuan,Longyan Zhao,Zhenghui Li,Chaligan Harqin,Yunfeng Peng,Jikai Liu
Phellinus igniarius is a well-known edible and medicinal fungus consumed in Asia for centuries. In the present study, a polysaccharide named PIP-1 was obtained from Phellinus igniarius mycelia, its physicochemical properties were determined, its detailed structures were elucidated by analysis of its depolymerized product, and its antioxidant and antitumor activities were evaluated. Results showed that PIP-1 had a high molecular weight, a high intrinsic viscosity and a linear repeating backbone composed of glucopyranose (Glc p ), galactopyranose, and mannopyranose joined by α-(1 → 4), α-(1 → 3), and α-(1 → 6) linkages, and single α‑terminal‑D‑Glc p as side chains 6- O -linked to the main chain according to the GC-MS and NMR results, and exhibited triple helical structure. Pharmacological results revealed that PIP-1 could effectively scavenge hydroxyl radicals, partly scavenge DPPH radials and chelate ferrous metal ions. Furthermore, PIP-1 showed potent inhibitory effects on growth of HT-29 and MCF-7 cells. These findings suggested that high-molecular-weight PIP-1 containing triple helical structure and only α-type glycosidic bond holds promise as novel functional foods and agents to promote human health.
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JOURNAL OF SOLID STATE CHEMISTRY
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2018
·
IF 2.179
Qi Tang,You Zhou,Li Ma,Mengyu Gan
The N-doped porous carbon/NiCo 2 O 4 (NPC/NiCo 2 O 4 ) substance is designed and synthesized via hydrothermal treatment , polymerization, carbonization , activation, and chemical bath deposition. The as-prepared NPC/NiCo 2 O 4 holds hemispherical flower-like structure that consists of two things: a porous bowl-shaped NPC at the inside, and sheet NiCo 2 O 4 at the whole outside, in which NPC works as a scaffold for NiCo 2 O 4 to restrain NiCo 2 O 4 nanosheets from aggregation in the synthesis process and NiCo 2 O 4 acts as a capacitive coating layer, and causing a synergic effect. The composite electrode has a specific capacitance of 948.30 F g −1 at a current density of 1 A g −1 , enhanced rate capability (727.43 F g −1 at 16 A g −1 ) and good conductivity. Additionally, NPC/NiCo 2 O 4 reaches a desirable cycling stability (87.4% capacitance retention after 2000 cycles even at a high current density of 10 A g −1 ), suggesting a hopeful electrode material for the electrochemical supercapacitors .
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RSC Advances
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2015
·Vol. 6
·期 7
·5305-5311
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IF 3.84
Lingfeng Gao,Li Ju,Hua Cui
The measurement of the binding constant of specific interactions and concentration of a target is of considerable importance in clinical diagnosis, therapy, bioassays and drug design. The development of methods that combine high sensitivity with generalization and simplicity for the measurement of both binding constant and target concentration is highly desirable. Previously, we developed a label-free chemiluminescence (CL) strategy for the measurement of the target concentration and binding constants between DNA aptamers and target simultaneously based on the fact that the target could enhance the CL produced by the reaction of the N-(4-aminobutyl)-N-ethylisoluminol (ABEI) functionalized gold colloid with H2O2. In this study, the enhancement and inhibition effect of various targets on the CL reaction is studied. The generalization of the proposed CL strategy for various targets is also explored. The results demonstrate that the proposed CL strategy is suitable for targets that can cause a change in CL intensity, which includes enhancement and inhibition. It could be applied for the measurement of the dissociation constants of aptamer-binding targets, antibody–antigen complexes, protein-binding small molecules and double-strand DNA hybrids from the millimole to picomole level. It could also be used for the sensitive determination of target concentration, including 2,4,6-trinitrotoluene (TNT), dopamine, tetracycline, human IgG (hIgG), tuberculosis (TB) DNA and mannose, with the detection limit of 0.93 nM–4.1 fM. This strategy is of great potential in fundamental research as well as in applications in life sciences.
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Journal of the American Chemical Society
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2017
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IF 13.858
Chunhong Chen,Haiyan Wang,Chuanlong Han,Jiang Deng,Jing Wang,Mingming Li,Minghui Tang,Haiyan Jin,Yong Wang
The soft template method is broadly applied to the fabrication of hollow-structured nanomaterials. However, due to the instability and the typical spherical shape of these soft templates, the resultant particles have a spherical morphology with a wide size distribution. Herein, we developed a sustainable route to fabricate asymmetric flasklike hollow carbonaceous structures with a highly uniform morphology and a narrow size distribution using the soft template method. A dynamic growth mechanism induced by the synergetic interactions between template and biomass is proposed. The precursors (ribose) provide an acidic environment for sodium oleate during the hydrothermal process in which oleic acid nanoemulsions are initially formed and serve as both template and benign solvent for the amphiphilic derivatives of the precursor. Simultaneously, the cosurfactant P123 facilitates the uniform dispersion of the nanoemulsion and is believed to cause the carbonaceous shells to rupture, providing openings through which the intermediates can enter. These subtle interactions facilitate the formation of the flasklike, asymmetric, hollow, carbonaceous nanoparticles. Furthermore, this unique structure contributes to the high surface area (2335 m2 g–1) of the flasklike carbon particles, which enhances the performance of supercapacitors. These findings may open up an exciting field for exploring anisotropic carbonaceous nanomaterials and for understanding the related mechanisms to provide guidance for the design of increasingly complex carbonaceous materials.
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ANALYTICA CHIMICA ACTA
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2016
·
IF 4.712
Xiangheng Niu,Yanfang He,Jianming Pan,Xin Li,Fengxian Qiu,Yongsheng Yan,Libo Shi,Hongli Zhao,Minbo Lan
Nanosized materials acting as substitutes of natural enzymes are currently attracting significant research due to their stable enzyme-like characteristics, but some flaws of these nanozymes , including their limited catalytic rate and efficiency, need to be remedied to enable their wider applications. In this work, we verify for the first time the catalytic behavior of uncapped nanobranch-based CuS clews as a peroxidase mimic. XRD , XPS , SEM, and TEM proofs demonstrate that high-purity CuS clews composed of intertwined wires with abundant nanodendrites outside are successfully produced via a facile one-pot hydrothermal synthesis approach, with thiourea as both the sulfion source and the structure-directing agent. The synthesized CuS can catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to trigger a visible color reaction with rapid response (reaching a maximum change within 5 min). The proposed CuS nanozyme exhibits preferable catalytic kinetics over natural horseradish peroxidase (HRP). This outstanding activity primarily results from the large surface area and rich sites exposed by the uncapped unique structure. Under optimized conditions, the fabricated sensing system provides linear absorbance (652 nm) changes in the H 2 O 2 concentration range of 0.2˜130 μM, with a detection limit of as low as 63 nM. When coupled with glucose oxidase (GOD), the system is demonstrated to be capable of monitoring glucose in blood samples with excellent performance.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2017
·
IF 3.582
Xiaoyan Li,Yingfang Yao,Jianguo Liu,Zhigang Zou
Nitrogen-doped carbon materials are known to be promising candidates as oxygen reduction reaction electrocatalysts used in fuel cells. However, developing metal-free catalysts with high performance and stability still remains a big challenge. Herein we report a new route by using the Maillard reaction , to caramelize ribose in a dispersing salt matrix, followed by carbonization of this caramel to synthesize metal-free catalysts. This catalytic material has the morphology of microporous nitrogen doped graphene-like carbon, and a highest surface area of 1261 m 2 g −1 with a large amount of micropores . Such microporous structure offers numerous defects that generate a large number of reactive sites. As a result, when used as the cathode materials in fuel cells, the fuel cell shows a high power density of 547 mW cm −2 under 1.0 atm back pressure with good stability with only 12.5% loss after 250 h. Such catalyst has good performance in the class of metal-free oxygen reduction reaction catalysts, and is possible for commercial use.
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ACS Sustainable Chemistry & Engineering
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2016
·
IF 5.267
Fan Xu,Yiqing Chen,Minghui Tang,Haiyan Wang,Jiang Deng,Yong Wang
Hydrothermal carbonization of biomass is an economic and sustainable technique for the fabrication of functional carbonaceous materials. However, the introduction of biomass into synthesis of ordered mesoporous carbon materials achieves slow progress due to complicated requirements. It is still challenging to prepare biomass-derived ordered mesoporous carbon materials. Herein, we report a simple and effective strategy to synthesize ordered mesoporous carbon materials from renewable and environment-friendly pentose using one-pot hydrothermal synthesis. The as-synthesized ordered mesoporous carbon materials show mesopores of two-dimensional hexagonal symmetry and rich functional groups. Mechanism studies indicate that the acid facilitates the self-assembly of carbon precursors and soft templates and therefore induced the formation of ordered mesoporous carbonaceous materials under hydrothermal conditions. This is the first report on the S0H+X–I+ self-assembly mechanism that achieves success in biomass system. In addition, the unique materials show outstanding performance as supports in catalytic hydrogenation. Significantly, this acid induced self-assembly strategy will open up new avenues for designing functional biomass-derived carbon materials.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jinghong Xu,Jing Zhang,Peihan Wu,Feifei Wang,Zongcai Tu,Hui Wang,Debin Guo
In this work, the effects of Maillard reaction of different monosaccharide-modified fish gelatin were studied. The changes of gel properties, rheology and structure of fish gelatin before and after modification were compared and analyzed, and oil-in-woter emulsions were prepared. The results showed that the five-carbon monosaccharide had stronger modification ability than the six-carbon monosaccharide, which was mainly due to the different steric hindrance of the amino acids in the nuclear layer and the outer layer to the glycosylation reaction. With the progress of the Maillard reaction, the color of fish gelatin gradually became darker. The attachment of sugar chains inhibited the gelation process of fish gelatin, decreased the gelation rate, changed the secondary structure, increased the content of β-turn or α-helix, increased the degree of fluorescence quenching, and enhanced the emulsifying properties and emulsion stability. This study provides useful information for the preparation of different types of monosaccharide-modified proteins and emulsions.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Nan Zhang,Chao Zhang,Yu Zhang,Zhongshuai Ma,Lingfei Li,Wei Liu
This study investigated the physicochemical properties, digestive stability, and in vitro fermentation behavior of Polygonatum kingianum polysaccharide (PKP) fractions (PKP60, PKP70, PKP80) obtained through graded ethanol precipitation. High-performance gel permeation chromatography revealed significant molecular weight differences among the fractions, while reverse-phase high-performance liquid chromatography indicated consistent monosaccharide types with variations in their proportions. Uronic acid analysis confirmed that all polysaccharide fractions met the criteria for neutral polysaccharides. Congo red staining confirmed the presence of a triple-helix structure in all PKP fractions. Comprehensive analysis demonstrated that these fractions remained stable during in vitro digestion, as evidenced by consistent molecular weights and total carbohydrate content, with no significant production of free monosaccharides or reducing sugars. All PKP fractions were fermented by gut microbiota, resulting in the production of short-chain fatty acids. Beta diversity and structural analyses of gut microbiota revealed distinct modulatory effects associated with each PKP fraction. The PKP fractions promoted probiotic growth, especially PKP70, which significantly enhanced Bifidobacterium proliferation, indicating strong prebiotic potential. These findings underscore the importance of isolation and purification methods in determining the functionality and gut microbiota-modulating effects of plant-derived polysaccharides, emphasizing the need for in-depth research that extends beyond merely evaluating their source.
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危险品化学品经营许可证（带存储）