SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jiawen Li,Huali Jin,Min Wei,Wenjie Ren,Jinshui Wang,Yurong Zhang,Ligen Wu,Baoshan He
In this work, a sensitive dual mode competitive electrochemical immunosensor based on differential pulse voltammetry (DPV) and square wave voltammetry (SWV) response modes for quantitative determination of dibutyl phthalate (DBP) was successfully established. According to diethylenetriamine (DETA) assisted hydrothermal strategy, a novel N-doped graphene/CoSe 2 nanobelt composite was prepared. Afterwards, PEI functionalized NG-CoSe 2 and gold nanowires (AuNWs) were further compounded as a preliminary signal amplification platform for immunosensor. Thionine (Thi)-loaded [email protected] and secondary antibody (Ab 2 ) connected as a signal label. The immobilized coating antigen DBP-BSA and the DBP compete for limited antibody binding sites. The concentration of DBP can be determined by DPV detect the Thi signal loaded on the [email protected] 2 label. It can also be determined by SWV mode to detect the current generated by the reaction system of H 2 O 2 and o-phenylenediamine (o-PD) catalyzed by [email protected] with peroxidase like properties. Under optimum conditions, the linear ranges of the DPV and SWV modes were 1 pg mL −1 ∼1 μg mL −1 . And LOD were 0.486 pg mL -1 (DPV) and 0.711 pg mL -1 (SWV), respectively. Besides, it exhibited outstanding selectivity, reproducibility, stability and can be used for sensitive detection of DBP in real samples.
研究领域:
分析化学
·
电化学
·
纳米技术
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 11
·1608
·
IF 4.8
Yuan Ji,Mingyue Fan,Bing Li,Guolin Gao,Zaixing Jiang
Covered stents have made a significant contribution to managing coronary artery perforation (CAP). Biocompatibility and toughness are critical properties for the covering membrane of covered stents. The mismatch between covered stents and patient coronary arteries in the clinic restricts the application of covered stents for emergency CAP. The ability to rapidly in situ coating of the stent at the rescue scene has so far been elusive, especially for small-diameter coronary artery covered stents. Here, we investigate a rapid coating technology of covered stents with polyvinylidene fluoride (PVDF)/dibutyl phthalate (DBP) covering membrane for CAP. The highly tough membrane and the short coating timeframe make it possible to prepare the covered stent suitable for patients with emergency CAP. In vitro cell assays demonstrated the excellent biocompatibility of the covering membrane. Moreover, in vivo evaluation in a rabbit model demonstrated successful delivery of the covered stent through the sheath system and effective sealing of vascular perforation.
研究领域:
生物医学工程
·
介入心脏病学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 11.3
Zhilong Xu,Kai Yu,Siyu Zhu,Yun Ju,Jing Zhao,Hong Zhang,Mingyi Wang,Jie Jiang,Jing He
Organophosphate diesters (di-OPEs) and hypochlorous acid (HOCl) are two typical indoor pollutants, which have been proved to negatively impact respiratory health. However, the underlying molecular mechanism of them acting on pulmonary phospholipids remains poorly understood. In this study, using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) as a model phospholipid, di-OPEs were found that can significantly amplify interfacial chemical damage induced by HOCl. This damage amplification effect can be observed in both alkyl- and aryl-di-OPEs, which shows concentration-dependence. This phenomenon can be attributed to two synergistic effects. Firstly, acidic phosphate group of di-OPEs lowers the system pH. This stabilizes HOCl in its undissociated neutral form, thereby preserving the highly oxidative reactivity of HOCl. Secondly, the lipophilicity of di-OPEs promotes their accumulation at the air-water interface, which elevates the H⁺ concentration in interfacial region. Such high interfacial concentration of H + aggravates the hydrolysis of POPG, thereby intensifying the overall chemical damage. Overall, this study offers a molecular level insight for clarifying the health implications of indoor di-OPEs in the presence of HOCl and lays the groundwork for evidence-based risk assessment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·Vol. 60
·期 31
·21582-21595
·
IF 12.2
Li Zhe,Wang Lei,Huang Fuxin,Han Siyue,Zhang Ying
Extensive import of mixed phthalate esters (PAEs) threatens
Mollisol
ecosystems. Nevertheless, mechanisms through which plant roots orchestrate
the cometabolic degradation of PAEs and couple this with soil humification
remain unclear. This study integrated plant physiology, untargeted
metabolomics, and genome-resolved metagenomics to decipher response
trajectories of the Pak Choi–Mollisol root–microbe system
across a mixed PAEs gradient. The findings indicated that under mild
stress (5 mg/kg), plants sustained a “growth-driven”
homeostasis alongside a stable rhizospheric microbiome. In contrast,
acute toxicity (20 mg/kg) initiated pronounced metabolic shifts, characterized
by a “survival-overgrowth” strategy. Specifically, carbon
fluxes shifted from primary assimilation to secondary defense hubs.
This physiological tradeoff reversed the root exudate profile, shifting
from basic carbohydrates to massive efflux of specific organic acids
and phenolics. Critically, these allelochemicals functioned as exogenous
elicitors, selectively recruiting specific metagenome-assembled genomes
(MAGs). Genomic evidence confirmed that single keystone MAGs (Pseudomonas
and Burkholderia) coharbored pcaG/H and extracellular laccase genes,
establishing a self-contained cometabolic module for concurrent PAEs
degradation and carbon stabilization. Consequently, based on correlative
multiomics evidence, extensive carbon efflux was associated with a
putative coupling between PAEs detoxification and stable humic carbon
accumulation, as indicated by a 48.7% increase in the humification
index (HIX). Ultimately, this multiomics framework elucidates the
plant-driven enhancement of carbon sinks within contaminated soils.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhenghao Sun,Tianye Wang,Guankai Qiu,Ningning Song,Xiutao Yang,Guopeng Zhu,Boling Deng,Quanying Wang,Hongwen Yu
Dibutyl phthalate (DBP) accumulation threatens soil microbial communities, yet its effects on microbial assembly mechanisms remain unclear. This study investigated the impacts of DBP with a wide range of level (i.e., 0–40 mg kg −1 , DBP0, DBP10, DBP20, and DBP40) on microbial community assembly processes in black soil of Northeast China through 90-day controlled laboratory incubations. A framework of infer community assembly mechanisms by phylogenetic bin-based null model analysis (iCAMP) was used to qualify the soil community assembly processes. The findings demonstrated significant reductions in soil enzyme activities (urease, β-glucosidase) and respiration intensity with elevated DBP concentrations. Microbial α-diversity declined significantly, with Shannon and Chao1 indices decreased by 1.79 % and 4.78 % for DBP10, 5.29 % and 9.70 % for DBP20, 14.66 % and 10.22 % for DBP40, respectively. The abundance of bacteria linked to soil carbon and nitrogen cycling exhibited reduction, such as Nocardioides (decreased from 1.24 % to 0.52 %) and Gp6 (decreased from 5.65 % to 3.67 %). Conversely, the relative abundances of bacteria which are associated with DBP degradation increased, such as Streptomyces (Increased from 20.21 % to 34.01 %) and Bacillus (Increased from 2.49 % to 5.21 %). DBP significantly altered microbial community assembly processes. As DBP concentration increased, the relative importance of homogeneous selection rose from 45.41 % to 55.63 %, while dispersal limitation declined from 48.89 % to 35.09 %. DBP homogenizes environmental conditions and a transition from neutral diffusion dynamics to pressure-driven selection, favoring taxa adapted to DBP degradation. This study advances understanding of DBP’s ecological impacts on soil microbial communities and their assembly dynamics.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Hong-Jia Huang,Bogui Pan,Qi-Feng Liang,Wan-Ting Zhang,Yan-Wen Li,Lei Xiang,Hai-Ming Zhao,Bai-Lin Liu,Quan-Ying Cai,Nai-Xian Feng,Ce-Hui Mo
Phthalates (PAEs) are emerging organic pollutants that threaten agroecosystems by diminishing agricultural productivity and posing risks to human health. However, traditional organic fertilizers have limited effectiveness in remediating PAE-contaminated soils, while bacterial remediation often lacks stability in heterogeneous soil environments. Here, a novel bio-organic fertilizer (N-BOF) containing the PAE-degrading Bacillus megaterium YJB3 was evaluated in pot experiments for its effectiveness in remediating PAEs-contaminated soils. Results revealed that N-BOF significantly reduced PAE concentrations in soil. At PAE levels of 20 and 100 mg/kg, N-BOF reduced DBP and DEHP concentrations by over 96 %, with a maximum reduction of 99.39 %. Additionally, N-BOF significantly enhanced soil physicochemical properties, increasing organic matter (OM), total organic carbon (TOC), cation exchange capacity (CEC), nutrient availability, and pH. N-BOF also improved the soil bacterial community, enhancing diversity, degradation capacities, and the abundance of aromatic compound-degrading bacteria. Ecological risk assessments demonstrated that N-BOF effectively mitigated PAE contamination risks, reducing the risk quotients (RQ) of DBP and DEHP to safe levels. This study provides practical strategies and theoretical insights for the sustainable remediation of PAE-contaminated soils, utilizing composting to simultaneously improve soil fertility and effectively eliminate PAEs.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Yuanyang Yi,Yuxian Wang,Wanqin Liu,Jing Zhu,Meiying Gu,Qiong Jia,Xue Li,Munire Mutalifu,Ling Jiang,Wei Zhang,Zhidong Zhang
Introduction: Di-n-butyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) and is considered an emerging global pollutant. It may pose a significant threat to ecosystem and human health due to its residual hazards and accumulation in the environment. Bacteria-driven PAE biodegradation is considered an economical and effective strategy for remediating such polluted environments.Methods: A DBP-degrading bacterium (P-7), was isolated from long-term film mulched cotton field soil. Its identity was confirmed via physiological, biochemical, and 16S rRNA gene analyses. The degradation conditions were optimized through single-factor experiments and response surface methodology (RSM).Furthermore, the whole-genome sequencing coupled with metabolomics was employed to elucidate metabolic mechanisms.Results: Priestia megaterium P-7 (P. megaterium P-7) achieved 100% DBP removal within 20 h under optimal conditions and exhibited broad substrate specificity for other PAEs. Genomic analysis identified key genes (lip, aes, ybfF, estA, and yvaK) encoding esterases/hydrolases that initiate DBP catabolism, converting it to phthalic acid (PA). Subsequent decarboxylation (pdc, bsdCD, mdcACDH, and lysA) and dioxygenase-mediated steps integrated PA into the TCA cycle. Metabolomics revealed three degradation pathways: decarboxylation (DBP → MBP → BB → BA→Catechol), hydrolysis (DBP → MBP → PA → PCA → Catechol) and direct β-oxidation (DBP → DEP → MEP → PA → Catechol).Conclusion: P. megaterium P-7 demonstrates exceptional degradation efficiency, substrate versatility, and environmental stress tolerance, making it a promising candidate for bioremediation of organic pollutants in contaminated soil.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Min Sun,Jiaqing Feng,Yang Feng,Xubo Xin,Yali Ding,Juanjuan Feng
Background Because of the advantages of good selectivity , high sensitivity, and fast analysis, high performance liquid chromatography (HPLC) has become one of the modern analytical techniques in wide application range, such as biological analysis, environmental detection, pharmaceutical and food inspection, agriculture and other fields. The stationary phase greatly decides the chromatographic separation performance, so the development of novel stationary phase is most important for HPLC. Results Pyridyl conjugated microporous polymers (P-CMP) with one to four layers were modified on the surface of amino silica to obtain a novel core-shell material (SiO2@P-CMP) by the layer-by-layer assembly strategy and Chichibabin reaction . The relationship between the structure of SiO2@P-CMP and chromatographic performance was carefully investigated, and the retention mechanism was revealed. The interactions including π-π stacking, hydrophobic effect and hydrogen bond gradually enhanced with the increase of P-CMP layers on the silica surface. Compared with C18 column, SiO2@P-CMP columns displayed better separation selectivity for polycyclic aromatic hydrocarbons (PAHs). According to the relative retention values ( α ), the separation performance of SiO2@P-CMP columns ( α = 1.144–1.884) for PAH isomers and other analytes was obviously better than that of C18 column ( α = 0.998–1.487). Furthermore, the SiO2@P-CMP column with four layers was selected to separate different types of analytes (eight PAHs, four bisphenols , four estrogens and nine phthalates), and the peak order of analytes was different from that on the C18 column due to the influence of hydrogen-bonding and π-π interactions. The relative standard deviations (n = 10) of retention time and peak area on SiO2@P-CMP column were between 0.28 % and 1.98 %. Significance and novelty Pyridyl conjugated microporous polymer was introduced as the stationary phase for the first time in HPLC. The proposed column displayed better separation characteristics compared to Zorbax SB-C 18 column. It provided a new idea for the separation of small molecules and the development of chromatographic packing or extraction material.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Ling-Zi Xia,Li-Lan Liu,Jun-Zhe Yue,Zhen-Yu Lu,Jie Zheng,Ming-Zhe Jiang,Min Lin,Jiaming Liu,Hai-Tao Gao
Phthalates (PEs) are widely used plasticizers in polymer products, and humans are increasingly exposed to them. This study was designed to investigate the alleviative effect of phytochemicals quercetin (Que) against male reproductive toxicity caused by the mixture of three commonly used PEs (MPEs), and further to explore the underlying mechanism. Forty-eight male SD rats were randomly and evenly divided into control group, Que group, MPEs group and MPEs+Que group ( n = 12); The oral exposure doses of MPEs and Que were 450 mg/kg/d and 50 mg/kg/d, respectively. After 91 days of continuous intervention, compared with control group, the testes weight, epididymis weight, serum sex hormones, and anogenital distance were significantly decreased in MPEs group ( P < 0.05); Testicular histopathological observation showed that all seminiferous tubules were atrophy, leydig cells were hyperplasia, spermatogenic cells growth were arrested in MPEs group. Ultrastructural observation of testicular germ cells showed that the edges of the nuclear membranes were indistinct, and the mitochondria were severely damaged with the cristae disrupted, decreased or even disappeared in MPEs group. Immunohistochemistry and Western blot analysis showed that testicular CYP11A1, CYP17A1 and 17β-HSD were up-regulated, while StAR, PIWIL1 and PIWIL2 were down-regulated in MPEs group ( P < 0.05); However, the alterations of these parameters were restored in MPEs+Que group. The results indicated MPEs disturbed steroid hormone metabolism, and caused male reproductive injuries; whereas, Que could inhibit MPEs’ male reproductive toxicity, which might relate to the restored regulation of steroid hormone metabolism.
研究领域:
毒理学
·
药理学
·
生殖生物学
·
内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Mengjie Qin,Mengmeng Zhou,Dongxue Li,Xuejing Lou,Jiawen Zhu,Xiao Tian,Ning Zhang,Wende Ma,Minghua Lu
In order to improve the enrichment performance of parent covalent organic frameworks (COFs), boronic acid functionalized of COF (COF-B(OH) 2 ) was obtained by a simple standing method for the first time. The obtained COF-B(OH) 2 exhibited the new characteristics that were not possessed by pure COF and was employed as the solid phase microextraction (SPME) coating material for highly efficient enrichment of trace endocrine disruptors phthalates (PAEs). Compared to pure COF, the synergistic effect of the newly emerged unique pore structure and boric acid interaction sites, and the large specific surface area and the abundant benzene ring structure inherited by original COF framework endowed COF-B(OH) 2 with enhanced enrichment performance for PAEs. Combined with gas chromatography-mass spectrometry (GC–MS), COF-B(OH) 2 exhibited the good linearity over a wide concentration of 0.1–3000 ng l -1 with good coefficients (R 2 , 0.9916–0.9998) for PAEs. The developed method was successfully employed for detection of trace PAEs in milk and water samples, demonstrating high recoveries (90.6–111.3 %). This work provides a sustainable approach to developing high-performance materials for enriching environmental pollutants.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Yi Luo,Riji Qiu,Xingran Zhang,Fang Li
Reverse osmosis (RO) system has been increasingly applied for circulating cooling water (CCW) reclamation. Plasticizers, which may be dissolved into CCW system in plastic manufacturing industry, cannot be completely removed by the pretreatment prior to RO system, possibly leading to severe membrane biofouling. Deciphering the characteristics and mechanisms of RO membrane biofouling in the presence of trace plasticizers are of paramount importance to the development of effective fouling control strategies. Herein, we demonstrate that exposure to a low concentration (1 − 10 μg/L) of three typical plasticizers (Dibutyl phthalate (DBP), Tributyl phosphate (TBP) and 2,2,4-Trimethylpentane-1,3-diol (TMPD)) detected in pretreated real CCW promoted Escherichia coli biofilm formation. DBP, TBP and TMPD showed the highest stimulation at 5 or 10 μg/L with biomass increasing by 55.7 ± 8.2 %, 35.9 ± 9.5 % and 32.2 ± 14.7 % respectively, relative to the unexposed control. Accordingly, the bacteria upon exposure to trace plasticizers showed enhanced adenosine triphosphate (ATP) activity, stimulated extracellular polymeric substances (EPS) excretion and suppressed intracellular reactive oxygen species (ROS) induction, causing by upregulation of related genes. Long-term study further showed that the RO membranes flowing by the pretreated real CCW in a polypropylene plant exhibited a severer biofouling behavior than exposed control, and DBP and TBP parts played a key role in stimulation effects on bacterial proliferation. Overall, we demonstrate that RO membrane exposure to trace plasticizers in pretreated CCW can upregulate molecular processes and physiologic responses that accelerate membrane biofouling, which provides important implications for biofouling control strategies in membrane-based CCW treatment systems.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Yue Tao,Yao Wang,Yunhe Cui,Rui Sun,Bo Zhang,Jianhua Qu,Hongguang Cai,Ying Zhang
To address the contamination caused by DBP residues prevalent in black soils, this study developed a multifunctional bioremediation material (BHF@DK-P3) using humic acid and iron-modified corn stover biochar in combination with microbiota. The microbiota contained DBP-degrading bacteria ( Enterobacterium sp. DNB-S2), phosphorus-solubilizing bacteria ( Enterobacter sp. P1) and potassium-solubilizing bacteria ( Paenibacillus sp. KT), and formed a good mutualistic symbiosis. In the biochar microenvironment, the microflora had lower DBP biotoxicity responses and more cell membrane formation. The addition of BHF@DK-P3 brought the structure of the DBP-contaminated black soil closer to the optimal three-phase ratio. The microbiota was able to perform their biological functions stably under both DBP stress and acid-base stress conditions. The stability of soil aggregates and the efficiency of N, P, K nutrients were improved, with available phosphorus increasing by 21.45%, available potassium by 12.54% and alkali-hydrolysable nitrogen by 14.74%. The relative abundance of copiotrophic bacterial taxa in the soil increased and the relative abundance of oligotrophic bacterial taxa decreased, providing a good mechanism for the conversion and utilization of soil nutrients. Biochar and microbiota jointly influenced soil carbon and nitrogen metabolism in response to DBP.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）