CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Haichao Zhao,Tian-Yu Sun,Liang-Feng Huang,Jiayu Wei,Shihui Qiu
Carbon dots as a specific type of carbon material require the connection of experiment to theory, exploration of new application, and development of new material. Here we present a new nitrogen-doped polymer nanodots (N-PDs) from 3,4-dihydroxy-L-phenylalanine (L-DOPA) via the one-step synthesis of simultaneous self-polymerization and carbonization process. This water-dispersible N-PDs, composed of poly-L-DOPA aggregates and embedded nitrogen-doped graphene patches, shows the zero-dimensional structure with an average diameter of 2.06 nm. In-depth investigation of the inhibition effect of as-prepared N-PDs demonstrates that N-PDs can suppress the oxygen and chloride corrosion of mild steel in extremely acidic condition through the dual effect of adsorption/assembly onto metal surface as a passivation film and strong covalent bonding with iron, resulting in an inhibition efficiency of 97.95% at the concentration higher than 200 mg/L within 6 h, the most efficient reported for carbon dots-based corrosion inhibitors. Furthermore, density-functional-theory calculations clearly reveal that the atoms present in unsaturated bonds of N-PDs play a dominant role in strengthening the interaction between corrosion inhibitor and metal substrate, which gives rise to the stable and homogeneous adsorption of N-PDs and then the effective protection for metal substrate against corrosion.
研究领域:
材料科学
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腐蚀与防护
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理论计算
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DOI
高影响因子
RSC Advances
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2016
·Vol. 6
·期 14
·11536-11545
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IF 3.289
Liqi Dong,Long Zhang,Xuemin Duan,Daize Mo,Jingkun Xu,Xiaofei Zhu
In this work, we present a pair of chiral PEDOT derivatives named poly((R)-2-(chloromethyl)-2,3-dihydrothieno[3,4-b][1,4]dioxine) ((R)-PEDTC) and poly((S)-2-(chloromethyl)-2,3-dihydrothieno[3,4-b][1,4]dioxine) ((S)-PEDTC), which were employed as excellent chiral recognition materials for fabricating chiral sensors and then discriminating between 3,4-dihydroxyphenylalanine (DOPA) enantiomers. Importantly, the mechanism of the stereospecificity of the interaction between the DOPA enantiomers and chiral polymers was discussed specifically. A series of performances of corresponding polymers were characterized in some detail using different strategies including CV, CD, FT-IR, UV-vis, SEM, and TG. The CD spectrum indicated that (R)-PEDTC and (S)-PEDTC are mirror symmetric. CV shows that the polymers had superior redox reversibility in CH3CN–Bu4NPF6. Finally, different electrochemical methods including CV, square wave voltammetry (SWV) and differential pulse voltammetry (DPV) were introduced for the discrimination of DOPA enantiomers. Satisfactory measurement results demonstrated that (R)-PEDTC/GCE and (S)-PEDTC/GCE exhibited excellent enantioselectivity of DOPA enantiomers and the tendency was for anisotropic interaction between (R)-PEDTC and L-DOPA, and (S)-PEDTC and D-DOPA. This implied that the obtained polymer films could be promising candidates as enantioselective materials in the electrochemical sensor field.
研究领域:
材料科学
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电化学
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生物医学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Jiaxue Liang,Hang Qi,Feng Zhu,Shuqi Chen,Bohua Liu,Chongling Sun,Yanyan Wang
Real-time monitoring the drugs penetration into brain is crucial for clinical therapy of neurodegenerative diseases. In this study, a Blood-Brain Barrier-electrochemical sensing (BBB-ES) platform combining BBB on chip model and electrochemical sensing system was proposed, which facilitated stable BBB growth under fluidic conditions and realized real-time detection of L-Dopa across the BBB. Based on this system, the effects of fluid force on the cell morphology, skeleton arrangement and tight junction protein ZO-1 of BBB growth were evaluated, it was verified that the fluid force promote cell rearrangement. Additionally, L-Dopa detecting performance was optimized by modifying SWNTs-AuNPs-PPy/Tyr(Single-Walled Carbon Nanotubes-Au Nanoparticles-Polypyrrole/Tyrosinase) on electrochemical electrode, which showed a wide linear response range of 10–500 μM and excellent selectivity toward L-Dopa. The integrated BBB-ES system was finally applied to real-time monitor the penetration process of L-Dopa through the BBB, it was proved that the penetration of L-Dopa increased with the increase of flow rate and time, and finding that L-Dopa was delivered by passive diffusion without disrupting the integrity of BBB. Based on these findings, the system can be used to monitor the real-time process of different drugs penetrating BBB and even different organ barriers, which also provide the possibility to solve the clinical demand for drugs screen.
研究领域:
神经科学
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生物医学工程
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药学
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电化学
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产品关联
DOI
高影响因子
Sensing and Bio-Sensing Research
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2025
·
IF 4.9
Jianjun Kang,Xue Lin,Chen Lin,Wuyuan Pan,Jian Wang,Fang Ke
Levodopa (L-DOPA) is a crucial neurotransmitter utilized in the treatment of neurological disorders, particularly in Parkinson's disease, where its concentration in biological fluids serves as a key diagnostic marker. Despite its significance, current methods for L-DOPA detection often suffer from limitations such as prolonged detection times, low sensitivity and weak anti-interference. This study addresses these challenges by introducing a highly efficient and colorimetric sensor for the rapid detection of L-DOPA, utilizing manganese-doped graphitic carbon nitride (Mn-g-C 3 N 4 ) as a peroxidase-mimetic catalyst. The sensor employs 3,3′,5,5′-tetramethylbenzidine as a substrate and achieves detection within 20 s, with a linear response over the concentration ranges of 1–10 μM and 20–90 μM, and the detection limits were 0.049 μM and 0.51 μM, respectively. The method has good specificity for L-DOPA and has been successfully applied to human serum even in the presence of interference from dopamine and other amino acids. In addition, the sensor exhibits satisfactory performance in smartphone-based L-DOPA detection. The novel sensor platform offers a simple, cost-effective, and scalable solution for real-time L-DOPA monitoring, with significant potential for applications in both clinical and environmental contexts.
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DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Shengwei Jia,Xiaoxia Li,Fang Wang,Hao Zhang,Qun-Dong Shen
Functional nanomaterials show great promise in next-generation targeted non-genetic neuromodulation and on-demand neurotransmitter release with superior spatiotemporal accuracy. L-DOPA-induced dyskinesia (LID) is a common complication during the treatment process of Parkinson's disease. Dysfunction in the dynamics of dopamine release plays a pivotal role in the disease. A strategy for LID treatment by using ultrasound-responsive functional nanomaterials for controlled release of nitric oxide is proposed, which can activate the nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic guanosine monophosphate (cGMP) signaling pathway. This alleviates the abnormal synaptic plasticity caused by excessive and pulsatile dopamine, and diminishes hypersensitivity of the D 1 dopamine receptors, thereby reducing the behavioral disorders associated with LID. These findings offer a novel perspective for therapy of neurodegenerative diseases.
研究领域:
纳米材料
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神经科学
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药理学
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神经退行性疾病
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Xue Hu,Yi Ding,Huan Liu,Zihan Pang,Mingjia Gao,Tian Liu
Dopa decarboxylase (DDC) is a key rate-limiting enzyme involved in cuticle tanning in insects, catalyzing the conversion of l-dopa to dopamine. Given its essential role in insect survival, DDC represents a potential target for pest control. In this study, we recombinantly expressed and characterized DDC from Drosophila melanogaster and developed a structural model for virtual screening. Quercetin (Que), the first identified inhibitor of insect DDC, was found to have an IC50 value of 9.43 μM. Notably, Que displayed much lower inhibitory activity against human DDC, with an IC50 value of 29.56 μM. Sequence-structure comparisons and molecular docking revealed several amino acid differences in the catalytic loop (L330, N326, V327, and D335) that may account for the distinct substrate specificity between insect and mammalian DDCs, as well as the differential inhibitor sensitivities. Bioassays demonstrated that Que exhibited significant insecticidal activity against the lepidopteran pest Plutella xylostella and the orthopteran pest Locusta migratoria. This study not only reinforces the potential of targeting insect DDC for insecticide development but also identifies a promising lead scaffold for further insecticide design.
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产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2016
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IF 4.843
Qing Chang,Rui Geng,Shanzheng Wang,Ding Qu,Xiangfei Kong
Treatment for multiple myeloma (MM) with a combined strategy of bone and tumor targeting remains a crucial technical challenge due to the incorporation of various functional components into one single system. Here, we developed dioleoyl phosphatidic acid (DOPA)-based paclitaxel (PTX)-loaded liposomes with modifications of alendronate and transferrin (Ald-/Tf-modified PTX-L), which were capable of bone affinity mediated by phosphate groups in DOPA and alendronate, and tumor targeting offered by transferrin. Ald-/Tf-modified PTX-L had clear and well-defined spherical shape with an intermediated size of 118.8 ± 4.8 nm, a highly negative surface charge of −46.9 ± 6.8 mV and a drug entrapment efficiency (DEE) of approximately 80%. When the pH was changed from pH 7.4 to pH 6.5, the accumulative release of PTX from Ald-/Tf-modified PTX-L significantly increased from 26.7 ± 3.7% to 41.7 ± 4.9%. Importantly, liposomes based on DOPA displayed an obviously stronger affinity with hydroxyapatite (HAp) than 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE)-based liposomes. Compared to PTX-L, Ald-/Tf-modified PTX-L exhibited obvious improvement of cytotoxicity (IC50 = 1.25 ± 0.09 μg/mL), significant enhancement on PTX intracellular accumulation (16.58 ± 0.62 μg/mg) and notable promotion to apoptosis induction (45.21 ± 3.10%) toward myeloma (MM1s) cells. In this study of antitumor efficacy, Ald-/Tf-modified PTX-L with bone-specific targeting showed a significant effect on extending the median survival time (48 days) and terminal survival time (> 58 days) against the MM1S-injected nude mice among all formulations. The results suggested that Ald-/Tf-modified PTX-L had potential as an efficient anticancer drug delivery system for MM therapy.
研究领域:
药学
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肿瘤学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
Microbial Biotechnology
·
2022
·Vol. 15
·期 5
·1610-1621
·
IF 6.575
Xing Liu,Xiao Han,Yuan Peng,Chunlin Tan,Jing Wang,Hongsong Xue,Ping Xu,Fei Tao
Summary 3, 4-Dihydroxyphenyl- l -alanine ( l -DOPA) is a compound of high medical value and is considered effective as a treatment for Parkinson’s disease. Currently, bioproduction of l -DOPA is mainly carried out by whole-cell catalysis mediated by recombinant Escherichia coli carrying heterogeneous tyrosine phenol lyase. Vibrio natriegens is increasingly attracting attention owing to its superiority, including extremely rapid growth and high soluble protein expression capacity. In this study, we attempt to develop an efficient whole-cell catalyst for l -DOPA production using V. natriegens as the chassis. The maximum soluble protein expression by V. natriegens was accomplished in 4 h at 37°C, which was equivalent to that achieved by E. coli in 16 h at 16°C. Furthermore, the maximum productivity reached over 10.0 g l −1 h −1 in the early stage of biocatalysis, nearly two-fold higher than previously reported. Approximately 54.0 g l −1 l -DOPA was obtained with a catechol conversion rate greater than 95%. In conclusion, V. natriegens displays advantages, including rapid protein expression and catalytic rate in the catalysis process for l -DOPA production. These findings strongly suggest that V. natriegens has remarkable potential as a whole-cell catalysis chassis for the production of valuable chemicals.
研究领域:
生物工程
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医学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2013
·
IF 4.75
Feifei Hou,Baoan Li,Minghao Xing,Qin Wang,Liang Hu,Shichang Wang
A novel composite hollow fiber membrane for membrane aerated biofilm rector (MABR) was prepared by coating L-3,4-dihydroxyphenylalanine (DOPA) on the surface of PVDF membrane. MABR process study was conducted to test the performances of the original and modified membranes for 166 days. The results indicate that coated membrane showed 2 times higher gas flux, lower water contact angle (declined from 86.5° to 52°), and significantly improved surface roughness. The modified membrane displayed an excellent MABR performance. Its COD, NH 4 + –N and TN removal efficiencies were kept above 90%, 98.8% and 84.2% during the first 4-month experiment. By tracking experiment at 0.01 MPa, to achieve COD removal efficiency of 85.9%, half an hour is required with the modified membrane, instead of 6 h using the original one. Besides, faster NH 4 + –N and TN removal at 0.01 MPa were also achieved with DOPA composite membrane.
研究领域:
环境工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
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2022
·
IF 3.191
Hang-Qin Zhu,Wen-Ye Hu,Xiao-Ling Tang,Ren-Chao Zheng,Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) exhibits great potential in industrial biosynthesis of l -tyrosine and its derivates. To uncover and screen TPLs with excellent catalytic properties, there is unmet demand for development of facile and reliable screening system for TPL. Here we presented a novel assay format for the detection of TPL activity based on catechol 2,3-dioxygenase (C23O)-catalyzed reaction. Catechol released from TPL-catalyzed cleavage of 3,4-dihydroxy- l -phenylalanine ( l -DOPA) was further oxidized by C23O to form 2-hydroxymuconate semialdehyde, which could be readily detected by spectrophotometric measurements at 375 nm. The assay achieved a unique balance between the ease of operation and superiority of analytical performances including linearity, sensitivity and accuracy. In addition, this assay enabled real-time monitoring of TPL activity with high efficiency and reliability. As C23O is highly specific towards catechol, a non-natural product of microorganism, the assay was therefore accessible to both crude cell extracts and the whole-cell system without elaborate purification steps of enzymes , which could greatly expedite discovery and engineering of TPLs. This study provided fundamental principle for high-throughput screening of other enzymes consuming or producing catechol derivatives.
研究领域:
生物化学
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工业生物技术
·
酶工程
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Keguang Mu,Dalun Zhang,Ziqiang Shao,Dujian Qin,Yalong Wang,Shuo Wang
l -Dopa functionalized halloysite nanotubes (HNTs) were prepared by the self-polymerization of l -dopa in the weak alkaline condition. Then different contents of l -dopa coated HNTs (LPDHNTs) were blended into cellulose acetate to prepare enhanced performance ultrafiltration membranes via the phase inversion method . The HNTs and LPDHNTs were characterized by FTIR, XPS, and TEM anysis. And the membranes morphologies, separation performance, antifouling performance, mechanical properties and hydrophilicity were also investigated. It was found that the composite membranes exhibited excellent antifouling performance. The pure water flux of 3.0 wt% LPDHNTs/CA membrane increased from 11.4 L m −2 h −1 to 92.9 L m −2 h −1 , while the EA rejection ratio of the membrane was about 91.2%. In addition, the mechanical properties of the resultant membranes were strengthened compared with the CA ultrafiltration membrane.
研究领域:
材料科学
·
高分子科学
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膜技术
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生物医学工程
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环境功能材料
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DOI
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 2
·e202203646
·
IF 2.1
Siying Ren,Wen-Chao Geng,Xuexian Cui,Bian Wu,Zhe Zheng
Graphical We present a robust supramolecular tandem assay to monitor L-DOPA decarboxylase activity and screen enzyme inhibitors using cucurbit[7]uril and acridine orange reporter pair. L–DOPA decarboxylase (DDC) deficiency causes serious progressive neurodegenerative disorders. Developing a sensitive enzyme activity monitoring and inhibitors or activators screening approach is highly on demand for DDC deficiency diagnosis. Here, we report a simple and sensitive fluorescence supramolecular tandem assay based on reporter pair of cucurbit[7]uril and acridine orange for DDC activity monitoring continuously. Furthermore, this strategy demonstrates the potential of STA to screen inhibitors of DDC.
研究领域:
生物化学
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医学诊断
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药物开发
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）