BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-14
·
IF 4.8
Chen Jing,Li Shoujie,Si Can,Liang Hanzhi,Duan Jun,He Chunmei
Background Abscisic acid (ABA) plays a crucial role in plant growth, development, and stress responses. The pyrabactin resistance-like (PYL) ABA receptor proteins, which are key components of ABA signal recognition and transmission. Dendrobium officinale is a precious traditional Chinese medicinal plant with known drought resistance. Nevertheless, the PYL genes of D. officinale, along with other orchid plants, remains unanalyzed.Results In this study, the PYL family was identified from D. officinale, consisting of 11 PYL proteins. A phylogenetic analysis showed that these DoPYL proteins were classified into three subfamilies. All the DoPYL proteins featured both Motif 1 and Motif 2. Notably, the members of subfamily I and III were intron-less, while those of subfamily II consistently displayed 2 introns. Analysis of expression patterns revealed that these DoPYL genes exhibit diverse spatiotemporal expression profiles in different organs. DoPYL1, DoPYL2, DoPYL4, DoPYL8 DoPYL9 and DoPYL10 followed a decreasing trend as protocorm-like bodies (PLBs) developed into plantlets. Under ABA and mannitol treatments, DoPYL1, DoPYL2, DoPYL4, DoPYL5, DoPYL6 and DoPYL10 exhibited significant down-regulation, while the expression of other genes remained unchanged. Following cold and salinity treatment, there was a notable up-regulation in the expression of the majority of DoPYL genes. PEG-simulated drought stress treatment led to the down-regulation of DoPYL genes. Furthermore, the DoPYL1 protein was localized in the cell membrane, cytoplasm and nucleus. During Yeast two-hybrid Library screening, we identified 40 potential interacting proteins of DoPYL1. Yeast two-hybrid verification confirmed that DoPYL1 interacted with DoRpb3, DoUbox, DoGrx, and DoATPsyn proteins.Conclusion Our results not only contribute to an understanding of the potential role of PYL proteins in growth, development and stress responses in Orchidaceae plants, but also provide additional theoretical guidance for breeding new varieties of D. officinale with resistance to abiotic stresses.
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高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Peiyi Wang,Baoming Tian,Zhiwei Ge,Jicai Feng,Jian Wang,Kai Yang,Peilong Sun,Ming Cai
Ultrasound and deep eutectic solvent (DES) are two green and efficient techniques in extraction. In this study, an eco-friendly extraction of phenolic compounds from Dendrobium officinale leaves (DOLs) was investigated. Ten DESs containing choline chloride, betaine , organic acids, polyols and amide, were tailored for extraction. Choline chloride-oxalic acid showed the most promising efficiencies for phenolics as 9.84 ± 0.19 mg GAE/g DW. Ultrasound-assisted DES (UADES) were optimized by a Box-Behnken design of response surface methodology. The optimum conditions were ultrasonic power 330 W, extraction temperature 60 ℃, and liquid-solid ratio 53:1 mL/g with a total phenolic content (TPC) of 17.50 ± 0.35 mg GAE/g DW. Compared with a single or triple frequency ultrasound, a dual-frequency of 40/60 kHz treatment for 15 min made TPC up to be 25.15 ± 1.46 mg GAE/g DW. About 13 phenolics were identified by UPLC-Triple-TOF/MS as the crucial phenolics in extracts , such as rutin and apigenin flavonoids . In addition, leave surface changed dramatically by UADES. These phenolic extracts had eminent antioxidant capacities, such as DPPH• scavenging rate of 65.4% and FRAP value of 0.234 mmol/L FeSO 4 at TPC of 4 mg/mL. Accordingly, dual-frequency UADES is a meaningful and green technology for extraction of phenolic compounds.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ming Cai,Hua Zhu,Lei Xu,Jian Wang,Jing Xu,Zhenhao Li,Kai Yang,Jianyong Wu,Peilong Sun
This study was to investigate the antifatigue, prebiotic effects and their relationships to the structure properties of three ethanol precipitated polysaccharides from Dendrobium officinale (EPDO), as EPDO-40, EPDO-60 and EPDO-80. EPDOs with anti-fatigue activity were screened out by forced swimming test, and blood lactic acid (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), liver glycogen, muscle glycogen, and intestinal microflora were investigated. Results showed that purified EPDO-60, 277.3 kDa, with a backbone consisted of 4-Man p and 4-Glc p . EPDO-60 had the best anti-fatigue activity, because it could significantly prolong the forced swimming time, as well as down-regulating the levels of BLA and BUN, increasing SOD. Proportions of Bacteroidetes and Firmicutes and abundance of Lactobacillus and Bifidobacterium in gut microflora increased after treated with EPDO-60. Accordingly, EPDO-60 could affect the community structure of gut microflora, leading to promote the balance of oxidation and antioxidation, and accelerated the fatigue metabolism in vivo .
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Aging-US
·
2023
·
IF 5.2
Huahua Li,Jin Zheng,Yacen Wu,Hong Zhou,Suli Zeng,Quanqing Li
Backgrounds: High glucose (HG) caused oxidative stress and mitochondrial dysfunction, resulting in insulin resistance in podocytes, a key mechanism of diabetic nephropathy. Dendrobium officinale polysaccharide (DOP) was able to improve insulin resistance and antioxidant capability. Objective: The purpose of this study is to explore the mechanism by which DOP decreases the podocyte injury induced by HG. Methods: MPC5 cells were treated with HG, DOP, and IRS-1/2 inhibitor NT157. Afterwards, glucose consumption, generations of ROS and MDA were measured using the detection kits. Mitophagy was monitored using both MtphagTracyker and LysoTracker. The mitochondrial membrane potential was evaluated by JC-1 staining. DOP was also used in a mouse model of diabetes, with the measurements of urine albumin, blood creatinine and blood urea nitrogen. Results: Treatment with DOP suppressed the HG-induced reduction of glucose consumption, the phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473 and Thr308) and Nephrin. In addition, HG-induced augment of ROS and MDA, formation of γ-H2A.X foci and translocation of AKT to nucleus were inhibited by DOP. DOP enhanced mitophagy, which was associated with decreased mitochondrial membrane potential and ROS production. DOP conferred protective effect on podocyte in the diabetic mouse by reducing the albumin/creatinine ratio and blood urea nitrogen, and restoring Nephrin expression in podocytes. Conclusions: DOP alleviates HG-induced podocyte injuryby regulating IRS-1/AKT signal and promoting mitophagy.
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高影响因子
Food & Function
·
2021
·Vol. 12
·期 12
·5524-5538
·
IF 5.396
Bo Li,Xinglishang He,Hai-Ying Jin,Hui-Ying Wang,Fu-Chen Zhou,Ning-Yu Zhang,Dong-Ying Jie,Lin-Zi Li,Jie Su,Xiang Zheng,Rong Luo,Gui-Yuan Lv,Ning-Hua Jiang,Qiao-Xian Yu,Su-Hong Chen
Given the increasing global trend toward unhealthy lifestyles and dietary decisions, such as “over-consumption of alcohol, and high sugar and fat diets” (ACHSFDs), it is not surprising that metabolic hypertension (MH) is now the most common type of hypertension. There is an urgent, global need for effective measures for the prevention and treatment of MH. Improper diet leads to decreased short-chain fatty acid (SCFA) production in the gut, leading to decreased gastrointestinal function, metabolism, and blood pressure as a result of signaling through G-protein-coupled receptors (GPCRs), ultimately causing MH. Previous studies have suggested that Dendrobium officinale (DO) may improve gastrointestinal function, lower blood pressure, and regulate metabolic abnormalities, but it is not clear whether it acts on MH by increasing SCFA and, if so, how. In this research, it was observed that Dendrobium officinale ultrafine powder (DOFP) could lower blood pressure and improve lipid abnormalities in ACHSFD-induced MH model rats. Moreover, DOFP was found to improve the intestinal flora and increased the SCFA level in feces and serum, as well as increased the expressions of GPCR43/41 and eNOS and the nitric oxide (NO) level. An experiment on isolated aorta rings revealed that DOFP improved the vascular endothelial relaxation function in MH rats, and this effect could be blocked by the eNOS inhibitor L-NAME. These experimental results suggest that DOFP improved the intestinal flora and increased the production, transportation, and utilization of SCFA, activated the intestinal-vascular axis SCFA-GPCR43/41 pathway, improved vascular endothelial function, and finally lowered blood pressure in MH model rats. This research provides a new focus for the mechanism of the effect of DOFP against MH by triggering the enteric-origin SCFA-GPCR43/41 pathway.
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高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Zhenlan Xu,Lingxiangyu Li,Yi Xu,Shanshan Wang,Xiaoxia Zhang,Tao Tang,Jianzhong Yu,Huiyu Zhao,Shenggan Wu,Chunrong Zhang,Xueping Zhao
Dendrobium officinale Kimura et Migo ( D. officinale ) is a dual-use plant with both botanical medicine and food applications, drawing increasing attentions. Pesticides are inevitably applied on D. officinale in commercial artificial-sheltered cultivation, yet little is known about pesticide residue levels in D. officinale . A modified high through-put QuEChERS method coupled with HPLC-MS/MS was developed and validated to detect 76 pesticides in D. officinale . Graphitized multi-wall carbon nanotubes (g-MWCNTs) was selected as the clean-up sorbent, showing relative weak affinity to triazole fungicide having planar structure in their molecular and low matrix effects of pesticides in D. officinale samples compared to MWCNTs and pesticarb. The validated method was applied to analyze pesticide residues in 86 real D. officinale samples collected from three main producing provinces. 43 different pesticides were detected with highest residue of 6.11 mg/kg for dimethomorph. Given possible health risks related to pesticide residues, accordingly, risk assessment of human exposure to pesticides via intake of D. officinale was thus performed, indicating that the pesticide residue in fresh or dry D. officinale would not cause potential risk to human health either in the long-term or short-term. This work improved our understanding of potential exposure risk of pesticide multi-residues in D. officinale .
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高影响因子
Foods
·
2025
·Vol. 14
·期 3
·413
·
IF 5.1
Xinyu Zhang,Pai Peng,Qianying Ma,Shance Niu,Shande Duan,Yimeng Zhang,Xinzhong Hu,Xiaolong Wang
Dendrobium officinale (DO) is a well-known medicinal and edible plant, yet its impact on the quality of noodles has been infrequently reported. In this study, DO was incorporated into multi-grain flour in varying proportions (0, 2, 4, 6, 8%) to prepare noodles, and their quality was assessed. The percentage increase in DO decreased the cooking loss, whiteness, appearance, and taste of the noodles while simultaneously enhancing their water absorption, adhesiveness, smoothness, and starch digestion resistance. Lower supplemental levels of DO (2–4%) facilitated the water absorption of protein and the formation of a dense and extensive protein network surrounding the partially gelatinized starch, which was characterized by higher relative crystallinity. The highest sensory score (77.4) and greatest content of slowly digestible starch content (38%) were observed in the noodles containing 4% DO. Conversely, higher percentages of DO (6–8%) diluted and compromised the protein network in the cooked noodles, leading to water migration from protein to starch. The excessive polysaccharides from DO tended to complex with fully gelatinized starch, promoting starch aggregation and interactions between starch and non-starch components. This ultimately resulted in the highest adhesiveness and resistant starch content (34%) in the cooked noodles with 8% DO. These findings provide a reference for enhancing noodle quality by regulating the amount of DO added, thereby promoting the application of DO in cereal-based food products.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Jing-yi Zhang,Ying Guo,Jin-ping Si,Xiao-bo Sun,Gui-bo Sun,Jing-jing Liu
Dendrobium officinale is one valuable traditional Chinese medicine, which has skyscraping medicinal value. Polysaccharide is the main active ingredient in D. officinale ; its antioxidant activity is a hot research topic nowadays. Oxidative stress plays an important role in the pathological progress of a variety of cardiovascular disease, as one of key factors of cardiomyocyte apoptosis. This research adopts a model of H 2 O 2 induction-H9c2 cardiomyocytes apoptosis, aiming to study the effect of Dendrobium officinale Polysaccharide (DOP-GY) for cardiomyocyte apoptosis caused by oxidative stress and its possible mechanism. Our results showed that pretreatment of DOP-GY (low dose: 6.25 μg/mL, medium dose: 12.5 μg/mL, high dose: 25 μg/mL) followed by a 2 h incubation with 200 μM H 2 O 2 elevated the survival rate, cutted the LDH leakage, reduced lipid peroxidation damage, improved the activity of the endogenous antioxidant enzymes. In addition, the pretreatment of DOP-GY significantly inhibited the production of ROS, declined of the mitochondrial membrane potential, down-regulated pro-apoptosis protein and up-regulated anti-apoptosis protein. The protective effect was correlated with the PI3K/Akt and MAPK signal pathway. Collectively, these observations suggest that DOY-GY has the potential to exert cardioprotective effects against H 2 O 2 -induced H9c2 cardiomyocyte apoptosis.
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Journal of Functional Foods
·
2023
·
IF 5.6
Xiawen Yang,Wenting Ji,Zhihong Zhou,Junfeng Wang,Zheng Cui,Xianglin Pan,Huiping Hu,Zihao He,Zhijing Wu,Yu Zhang,Kaiping Wang
Liver fibrosis is a result of chronic liver disease; without treatment, it can progress to cirrhosis and even liver cancer. The occurrence and development of liver fibrosis is closely related to the activation of hepatic stellate cells (HSCs). Many events, including induction of the hedgehog signal and angiogenesis, occur during HSCs activation. Transforming growth factor-β (TGF-β) acts as a critical regulator of HSCs activation. We found that Dendrobium officinale polysaccharide (DOP) markedly reduced the level of hepatic fibrosis in carbon tetrachloride (CCl 4 )-treated rats and TGF-β-stimulated HSC-T6 cells. DOP significantly inhibited smooth (SMO) protein expression and Gli1 transcription into the nucleus. Moreover, DOP inhibited the expression of angiogenesis-related proteins and angiogenesis markers. In conclusion, our results indicated that DOP might ameliorate CCl 4 -induced liver fibrosis and TGF-β-induced HSC-T6 cells activation by the SMO/Gli 1 pathway, suggesting that DOP could be used as a potential functional food.
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Plants-Basel
·
2025
·Vol. 14
·期 11
·1576
·
IF 4.1
Zhangting Xu,Feixiong Zheng,Xiaoji Deng,Yiming Sun,Zhenming Yu,Xiaoxia Shen
Dendrobium officinale,which was rich in bioactive compounds such as polysaccharides, alkaloids, amino acids, and flavonoids, had significant medicinal value and ability to resist stresses. Studies had demonstrated thatGATAgenes were one of the crucial regulators in controlling plant development and growth and stress response. Genome-wide identification and characterization of the 18DoGATAgenes were displayed. According to phylogenetic relationships, theDoGATAfamily genes were divided into 4 groups and the conserved motifs ofDoGATA1–DoGATA18within the same group were similar. AllDoGATAgenes were localized in the nucleus and randomly mapped on 10 chromosomes. The GATA genes inD. officinaleexperienced one pair of tandem duplication and 4 pairs of segment duplications to expand the family genes. Additionally, we found that the 2000 bp upstream promoter region of theDoGATAgenes harbored 23 types ofcis-acting elements that were categorized into plant growth and development, phytohormone responsiveness, and stress responsiveness.DoGATA1–DoGATA18were diversely expressed across different tissues (root, leaf, stem, flower), exposed to salt stress, and following MeJA treatment. Co-expression analysis betweenDoGATAand enzyme-encoding genes involved in the biosynthesis of flavone showed thatDoCHI(LOC110104562) andDoGTMT(LOC110098370) may be potential downstream targets ofDoGATA16to regulate flavonoid biosynthesis to adapt to salt stress. Furthermore, we confirmed thatDoGATA16may act as a key member to resist stress. The collective findings of this study shed light on the function ofGATAgenes and molecular breeding ofD. officinale.
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高影响因子
Frontiers in Nutrition
·
2026
·
IF 5.5
Binhong Hu,Ling Yao,Xin Deng,Wei Zhao,Kuo Chen,Jiacheng Zhang,Zhiqiang Li,Jin-ping Li,Wei Jiang,Huabao Liu
AimsThis study aims to explore the potential therapeutic effect of Dendrobium officinale polysaccharide (DOP) on non-alcoholic fatty liver disease (NAFLD) induced by high-fat diet (HFD), and to elucidate the underlying mechanism involving the SIRT6/PGC-1α signaling axis and the regulation of the gut microbiota.MethodsWe extracted and characterized DOP. We established a rat model of NAFLD induced by HFD and evaluated the efficacy of DOP by integrating multi-omics techniques (transcriptomics, metabolomics) and 16S rRNA sequencing. To verify the specific role of SIRT6, we introduced the SIRT6 inhibitor OSS_128167 in the primary hepatocyte model induced by oleic acid/palmitic acid (OA/PA).ResultsDOP significantly alleviated liver steatosis, oxidative stress, and lipid metabolism disorders induced by HFD. Multi-omics analysis indicated that DOP regulated liver glycerophospholipid metabolism and restored intestinal microbiota homeostasis, significantly increasing the abundance of beneficial bacteria such as Lactobacillus. Mechanistically, DOP activated the liver SIRT6/PGC-1α signaling axis, thereby enhancing antioxidant defense and inhibiting lipogenesis. Crucially, in vitro experiments confirmed that the SIRT6 inhibitor OSS_128167 eliminated the protective effect of DOP on lipid accumulation, confirming that the effect of DOP depends on SIRT6.ConclusionDOP improves NAFLD through dual mechanisms of regulating the gut-liver axis homeostasis and directly activating the liver SIRT6/PGC-1α signaling pathway. The results of this study provide a theoretical basis for developing DOP as a drug for the treatment of NAFLD.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Minghui Sui,Simin Feng,Gaodan Liu,Bilian Chen,Zhenhao Li,Ping Shao
In this study, ultrasonic-assisted deep eutectic solvent (DES) method was used to extract flavonoids from Dendrobium officinale . The mechanism of DESs extraction differences was investigated by physicochemical characterization and molecular dynamics simulation experiments. Moreover, flavonoids from Dendrobium officinale were rapidly identified and quantitatively analyzed by UPLC-Triple-TOF/MS without removing DES. The results showed choline chloride-lactic acid (Chol-Lac) had the best extraction effect among forty groups of DESs, the extraction yield was up to 35.23 ± 2.12 mg/g. It was speculated that the tight hydrogen bond structure was the key to the better extraction ability of Chol-Lac. Meanwhile, the viscosity was the main physical parameter reflecting the hydrogen bond structure of DES. Fourteen flavonoid glycosides were identified, among which the content of rutin (1.94 ± 0.24 mg/g) was the highest. And the results of cluster analysis showed that different DESs had great differences in the extraction of flavonoid glycosides.
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高影响因子
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