JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 100
·期 11
·4319-4328
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IF 2.614
Tengfei Zhan,Yunsheng Han,Chaohua Tang,Qingyu Zhao,Dandan Sun,Ying Li,Xueting Jia,Lingyun Zhou,Junmin Zhang
BACKGROUND The aim of this study was to investigate the metabolism of α -tocopherol derived from vitamin E-enriched transgenic maize (VER) and its effects on antioxidant and immune functions in broilers aged 1–42 days. A total of 360 1-day-old male broilers were randomly divided into three groups containing six replicates with 20 broilers per replicate. The negative control (NC) group and the positive control (PC) group were given non-GM maize and non-GM maize plus exogenous vitamin E (VE), respectively, and the VER group was given VER, replacing the non-GM maize given to the NC group. Between days 1 and 21 and days 22 and 42, VE levels were 4.38 and 4.63 mg kg −1 in the NC group, and 14.11 and 14.91 mg kg −1 in the PC and VER group, respectively. RESULTS The results showed that α -tocopherol from both VER and additives increased α -tocopherol transfer protein and cytochrome P450 concentrations. Serum α -tocopherol and α -tocopherylquinone levels of broilers in the PC and VER groups were also significantly higher than those in the NC group ( P < 0.05). Compared with the NC group, broilers in both groups that received α -tocopherol had reduced NF- κ B p65 concentrations, significantly decreased serum prostaglandin E 2 , interleukin-6, malondialdehyde, and hydrogen peroxide levels ( P < 0.05), and significantly increased glutathione, glutathione peroxidase, and total antioxidant capacity ( P < 0.05). CONCLUSION In summary, both VER and non-GM maize fortified with exogenous VE showed similar effects on broilers, indicating that the α -tocopherol in VER has sufficient biological activity. © 2020 Society of Chemical Industry
研究领域:
动物营养
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基因工程
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2017
·Vol. 8
·期 7
·2601-2610
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IF 3.247
Meihui Fan,Guowen Zhang,Junhui Pan,Deming Gong
Dihydromyricetin (DMY), a natural flavonoid, was found to effectively inhibit tyrosinase activity in a mixed-type manner with an IC50 value of (3.66 ± 0.14) × 10−5 mol L−1. DMY combined with the dietary vitamin D3 at lower concentrations exhibited a synergistic effect on the inhibition of tyrosinase. The formation of a DMY–tyrosinase complex led to fluorescence quenching and conformational changes of tyrosinase, which was driven mainly by hydrophobic interactions and hydrogen bonds. The molecular simulation further found that DMY inserted into the active pocket of tyrosinase interacted with amino acid residues Tyr78, His85, and Ala323, occupying the catalytic center of tyrosinase to hinder entrance of the substrate, leading to the inhibition of tyrosinase. This study may provide a scientific foundation for screening effective tyrosinase inhibitors.
研究领域:
生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Lei Qin,Qian Zeng,Jingjing Zhang,Hongye Cheng,Lifang Chen,Zhiwen Qi
An enhanced process integrating an extraction with ionic liquid (IL) as solvent and a two-step reextraction is proposed to recover high purity vitamin E from the methylated oil deodorizer distillate (MODD). Conditions for the extraction with solvent [C 6 MIm]Ac were optimized and its reusability was investigated. A crude concentrated vitamin E phase (extract phase) was acquired with some co-extracted fatty acid methyl ester (FAME). In order to remove the co-extracted FAME and obtain the high purity vitamin E, a two-step reextraction process was designed, where n -hexane was proved to be the most promising solvent with the selectivity of 728.01 and partition coefficient of 18.92 to methyllinoleate. Based on the proposed process, high purity vitamin E could be finally obtained from both the model MODD (purity of 99.26%) and two different practical MODDs (purity > 79.08%) with [C 6 MIm]Ac as extractant and a small amount of n -hexane and water as reextractants. It means that the integrated extraction process with high performance is a promising method for recovery of vitamin E from MODDs.
研究领域:
食品科学
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化学工程
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DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Jing Chang,Jiahui Wang,Beibei Luo,Weihao Li,Ziyue Xiong,Chaoqi Du,Xue Wang,Yuejiao Wang,Jingya Tian,Shuxin Li,Yue Fang,Longjie Li,Jing Dong,Ke Tan,Yumei Fan,Pengxiu Cao
Introduction: Pulmonary fibrosis (PF) is a fatal chronic lung disease that causes structural damage and decreased lung function and has a poor prognosis. Currently, there is no medicine that can truly cure PF. Vitamin E (VE) is a group of natural antioxidants with anticancer and antimutagenic properties. There have been a few reports about the attenuation of PF by VE in experimental animals, but the molecular mechanisms are not fully understood. Methods: Bleomycin-induced PF (BLM-PF) mouse model, and cultured mouse primary lung fibroblasts and MLE 12 cells were utilized. Pathological examination of lung sections, immunoblotting, immunofluorescent staining, and real-time PCR were conducted in this study. Results: We confirmed that VE significantly delayed the progression of BLM-PF and increased the survival rates of experimental mice with PF. VE suppressed the pathological activation and fibrotic differentiation of lung fibroblasts and epithelial-mesenchymal transition and alleviated the inflammatory response in BLM-induced fibrotic lungs and pulmonary epithelial cells in vitro . Importantly, VE reduced BLM-induced ferritin expression in fibrotic lungs, whereas VE did not exhibit iron chelation properties in fibroblasts or epithelial cells in vitro . Furthermore, VE protected against mitochondrial dysmorphology and normalized mitochondrial protein expression in BLM-PF lungs. Consistently, VE suppressed apoptosis in BLM-PF lungs and pulmonary epithelial cells in vitro . Discussion: Collectively, VE markedly inhibited BLM-induced PF through a complex mechanism, including improving iron metabolism and mitochondrial structure and function, mitigating inflammation, and decreasing the fibrotic functions of fibroblasts and epithelial cells. Therefore, VE presents a highly potential therapeutic against PF due to its multiple protective effects with few side effects.
研究领域:
肺科学
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药理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2018
·Vol. 24
·期 3
·263-271
·
IF 0
Zhang Xubin,Hao Zhencheng,Yang Chaoqun,Ahishakiye Rosine,Wang Fumin
In this paper, 2,3,5-trimethyl-1,4-benzoquinone (TMBQ) was synthesized through the direct oxidation of 1,2,4-trimethylbenzene (pseudocumene, TMB) in the HCOOH–H 2 O 2 system. The influence of three active species was studied, including performic acid (PFA) generated in formic acid, peracetic acid (PAA) generated in acetic acid, and trifluoroperacetic (TFPA) acid generated in trifluoroacetic acid. The effects of sulfuric acid and sodium formate addition were investigated, the over-oxidation of TMB was discussed, and the main reason for the decreasing selectivity was revealed. The oxidation of TMB can be controlled and improved through adjusting the reaction temperature, mole ratio of oxidant to substrate, and reactant concentration. The TMBQ yield of 28% was achieved with a TMB concentration of 0.2 mol/L, H 2 O 2 /TMB mole ratio of 6:1, and reaction temperature 37 °C. The selectivity of 72% was obtained with a TMB concentration of 0.2 mol/L, H 2 O 2 /TMB mole ratio of 5:1, and reaction temperature of 27 °C. The reaction mechanisms were proposed and discussed based on the gas chromatography–flame ionization detection (GC–FID) and gas chromatography–mass spectrometer (GC–MS) results.
研究领域:
有机化学
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合成化学
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产品关联
DOI
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2024
·
IF 1.9
Wenlin Li,Sen Lin,Dongming Lan,Yonghua Wang
Vitamin E succinate has gained substantial attention as a potential therapeutic agent for cancer treatment due to its biomedical activities. One of the prominent methods of synthesizing vitamin E succinate is through enzymatic processes, which, although advantageous, presents inherent challenges related to optimization, scalability, and particularly, the poor stability of lipases in organic solvents. Our study addresses these challenges by conducting a comprehensive comparative analysis between Lipase UM1 and three other immobilized commercial lipases, demonstrating Lipase UM1's enhanced resistance to organic solvents and its superior efficiency in vitamin E succinate production. Further optimization experiments with Lipase UM1 led to an unprecedented conversion of 99%. Additionally, we scaled the reaction to a proof-of-concept industrial level. The synthesized product was verified using Fourier transform infrared spectroscopy and nuclear magnetic resonance analysis, ensuring its quality and consistency. This study validates Lipase UM1 as an efficient catalyst for vitamin E succinate synthesis, offering a promising avenue for industrial production with potential applications in cancer therapy and beyond. Graphical Vitamin E succinate (VES) was synthesized through the catalysis of the organic solvent-stable Lipase UM1, resulting in an exceptional conversion.
研究领域:
生物化学
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医学
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药学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Diqiong Wang,Mingming Zhong,Yufan Sun,Lin Fang,Yuanda Sun,Baokun Qi,Yang Li
Soybean lipophilic protein (LP) is a promising emulsifier for nanoemulsion delivery systems. Herein, the influence of pH on the stability, rheological properties, and encapsulation efficiency of a nanoemulsion formed by ultrasonically modified LP was determined, and its influence mechanism was explored. The delivery system exhibited a high absolute zeta potential value (18–30 mV) in neutral and alkaline environments (pH 7.0 or 9.0) and uniform particle size distribution (100–350 nm). Cryo-scanning electron microscopy showed that LPs in neutral and alkaline environments covered the oil–water interface more evenly than those in acidic environments. Moreover, the LP nanoemulsion has excellent rheological properties and exhibit high emulsification performance, vitamin E encapsulation efficiency, and oxidation stability. These improvements occurred because the nanoemulsion prepared using ultrasonicated LP had a high absolute zeta potential value (maximum 30 mV), small droplet size (minimum 190 nm), uniform protein film , high viscosity, and good viscoelasticity in different pH environments. In summary, the nanoemulsion delivery system prepared by LP ultrasonication can resist the influence of different pH environments and maximize the delivery efficiency in neutral and alkaline environments. The research results identified a suitable pH environment for LP nanoemulsions and provide a theoretical reference for their further application.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
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2024
·
IF 5.3
Yuge Long,Xiaoyuan Jia,Liang Chu
Gasdermin E ( GSDME ), which is also known as DFNA5 , was first identified as a deafness-related gene that is expressed in cochlear hair cells, and mutation of this gene causes autosomal dominant neurogenic hearing loss. Later studies revealed that GSDME is mostly expressed in the kidney, placenta, muscle and brain cells, but it is expressed at low levels in tumor cells. The GSDME gene encodes the GSDME protein, which is a member of the gasdermin (GSDM) family and has been shown to participate in the induction of apoptosis and pyroptosis. The current literature suggests that Caspase-3 and Granzyme B (Gzm B) can cleave GSDME to generate the active N-terminal fragment (GSDME-NT), which integrates with the cell membrane and forms pores in this membrane to induce pyroptosis. Furthermore, GSDME also forms pores in mitochondrial membranes to release apoptosis factors, such as cytochrome c (Cyt c ) and high-temperature requirement protein A2 (HtrA2/Omi), and subsequently activates the intrinsic apoptosis pathway. In recent years, GSDME has been shown to exert tumor-suppressive effects, suggesting that it has potential therapeutic effects on tumors. In this review, we introduce the structure and function of GSDME and the mechanism by which it induces cell death, and we discuss its tumor suppressive effect.
研究领域:
分子生物学
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细胞生物学
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肿瘤学
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遗传学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2024
·
IF 9.7
Weichun Gao,Shengnan Chen,Cong Geng,Yinyan Guan,Yingjun Gao,Jiyan Liang
Given the limitations of unequal H 2 O 2 and O 3 distribution in the E-peroxone (EP) process, a novel dual-wavelength UV enhanced E-peroxone process (VUV/UVC/EP) with high performance for removing emerging contaminants was established. In this paper, carbamazepine (CBZ) removal rates by different oxidation processes were investigated, calculating the kinetic effects of the VUV/UVC/EP processes in various operating parameters, and the degradation mechanisms and pathways of CBZ were comprehensively discussed. Compared with conventional EP, ultraviolet (UVC, wavelength 254 nm), UVC/EP, and dual-wavelength ultraviolet (VUV/UVC, wavelength 185 and 254 nm) processes, the VUV/UVC/EP process exhibits an excellent ability for degradation of carbamazepine (CBZ), the synergy coefficient between VUV/UVC and EP process is 1.44 (>1). In particular, the VUV/UVC/EP process exhibits exceptional degradation performance even at 50 mg L −1 CBZ. It also demonstrates high adaptability to varying pH, humic acid concentration, and different inorganic anions (SO 4 2− , Cl − , NO 3 − , PO 4 3− and CO 3 2− ). In addition, ·OH, 1 O 2 , and·O 2 − were identified to exist in the VUV/UVC/EP process. The dominant reactive species ·OH content was increased by decomposing the excess dissolved O 3 to accelerate CBZ degradation. CBZ was broken down into a series of intermediates, NO 2 − , NO 3 − , and other inorganic compounds through ring reduction and oxidation reactions under the attack of reactive oxygen species (ROS) generated in the VUV/UVC/EP process. It also can remove more than 50% of TOC in 30 min and decrease biological toxicity. An electrical energy-per-order (EEO) value of 3.37 kW h m −3 indicated that the VUV/UVC/EP process is economically feasible. This comprehensive examination provides valuable insights into the CBZ removal in reclaimed water, contributing to the advancement of environmental water resources.
研究领域:
环境科学
·
水处理技术
✓ 已验证
DOI
高影响因子
APPLIED THERMAL ENGINEERING
·
2025
·
IF 6.9
Yanjun Sun,Haiqi Zheng,Bohao Yin,Xiaopo Wang,Maogang He
With global energy consumption surging and environmental concerns escalating, absorption refrigeration systems are receiving increasing attention. This study investigates the absorption behavior and thermodynamic performance of absorption refrigeration systems using trans -1-chloro-3,3,3-trifluoro-1-propene (R-1233zd(E)) paired with phosphonium-based ionic liquids (ILs), trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl) amide ([P 66614 ][Tf 2 N]) and trihexyltetradecylphosphonium bis(2,4,4-trimethylpentyl) phosphinate ([P 66614 ][TMPP]). Experimental solubility measurements of R-1233zd(E) in the ILs were conducted using an isochoric saturation method across a temperature range of 293.15–343.15 K and pressures up to 0.61 MPa. The Non-Random Two-Liquid (NRTL) thermodynamic model was employed to correlate solubility data, yielding excellent agreement with deviations of less than 3.84 %. Results revealed that [P 66614 ][TMPP] exhibits higher solubility due to its branched alkyl chains, increased free volume, and favorable van der Waals interactions. Thermodynamic analyses were performed for single-effect (SARS), double-effect (DARS), and compression-assisted cycles (LC-SARS, single-effect cycle with a compressor added in the low-pressure side; HC-SARS, single-effect cycle with a compressor added in the high-pressure side; LC-DARS, double-effect cycle with a compressor added in the low-pressure side). It demonstrated that [P 66614 ][TMPP] exhibits a higher coefficient of performance (COP) compared to [P 66614 ][Tf 2 N]. Compression assistance reduced the minimum generation temperature and improved COP by leveraging electrical energy. Among all configurations, LC-DARS with a compression ratio of 1.9 demonstrated the optimal balance between COP (0.82) and thermal energy utilization efficiency. These findings highlight the potential of R-1233zd(E)/[P 66614 ][TMPP] as a sustainable working pair for absorption systems, particularly in low-grade heat recovery applications.
研究领域:
工程热物理学
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化学工程
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2018
·
IF 3.466
Wen-Bin Zhao,Chi-Xiao Qiu,Ying Shen,Wen-Hui Liu,Jie Zhou,Ying-Chun Xu,Zhan Zhou,Shu-Qing Chen
Antibody-drug conjugates (ADCs) consist of cytotoxic agents covalently conjugated to monoclonal antibodies that substantially improve antitumour activity and reduce systemic toxicity. With the growing number of ADCs in clinical applications, more accurate bioanalysis data are urgently needed to facilitate the development and rational use of ADCs. Herein, we used antigen-positive cells as antigen carriers and ofatumumab (OFA-HL) and ofatumumab-based ADC (OFA-HL-MMAE) as examples to establish a new ligand-binding assay (LBA) method based on flow cytometry. We proved that the new method met the required analytical performance criteria and the lower limit of quantitation (LOQ) was 0.2 μg/mL. In addition, the LOQ of the quantitative OFA-HL flow cytometry method was reduced to 0.025 μg/mL by choosing an optimized fluorescent antibody, which indicated that the LOQ of the new method can be improved. What's more, the new method showed good stability and specificity when we used it to determine the concentrations of OFA-HL and OFA-HL-MMAE in mouse serum. During the bioanalysis of ADCs, various factors should be considered. Therefore, choosing optimal methods for ADC bioanalysis is necessary. This new method using in situ antigens not only extends the scope of application of the conventional LBA methods by avoiding the need for soluble antigens, but also improves the authenticity of ADC bioanalysis as a supplementary approach, which is valuable for developing accurate ADC assays.
研究领域:
药理学
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肿瘤学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Jinfeng He,Shuqian Yu,Ziyi Ma,Haotian Sun,Qinghua Yang,Zhiwei Liu,Xuan Wang,Xiaoyu Zhang,Longgang Wang
Gold nanoparticles are a kind of good photothermal agents. However, gold nanoparticle photothermal agents have low photothermal conversion efficiency and low tumor inhibiting ability. To overcome these problems, polymyxin E (PE) biomineralized and doxorubicin-loaded gold nanoflowers (DOX-SH@AuNFs) nanodrug was synthesized by the green synthesis method using the biological antimicrobial peptide PE as a stabilizer and grower of crystal seed, and doxorubicin-thiol (DOX-SH) was further loaded on the gold nanoparticles through the Au-S bond. The final DOX-SH@AuNFs displayed a wide absorption band in the UV–Vis spectra, and their photothermal conversion efficiency was 33.9%. Furthermore, the inhibition rate of DOX-SH@AuNFs on A549 cells was as high as 80.1% under the irradiation of near-infrared light at 808 nm. The tumor inhibition rate of DOX-SH@AuNFs was as high as 87.81% in vivo experiments. The high tumor suppression rate was attributed to the high photothermal conversion ability of DOX-SH@AuNFs and the delivery of doxorubicin. Taken together, the method of preparing DOX-SH@AuNFs provides a new idea for the treatment of cancer by photothermal therapy and chemotherapy synergistic system.
研究领域:
纳米技术
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癌症研究
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药学科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）