SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kai Zhang,Meina Yang,Helin Sun,Xueyin Wang,Hao Li,Jinxiang Han,Zhongwen Zhang
Using Peptide nucleic acid (PNA), this work provides a unique approach for controlling CRISPR/Cas13a activity by RNA Inhibitory PNA (RIP). This method reduces the action of CRISPR/Cas13a by inhibiting the binding of crRNA to CRISPR/Cas13a, which was subsequently utilized to detect metalloproteinase-2 (MMP-2) activity. We carefully constructed a PNA sequence that includes a peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) that is particularly cleaved by MMP-2 in the middle. When MMP-2 is not present in the system, the PNA/crRNA complex reduces crRNA binding to CRISPR/Cas13a, hence inhibiting CRISPR/Cas13a cleavage activity. When MMP-2 is present in the system, it cleaves the peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) selectively and so prevents PNA from binding to crRNA, enabling crRNA to reach the "free" form. Then, in the presence of an RNA activator, crRNA may bind to CRISPR/Cas13a and stimulate its activity. CRISPR/Cas13a triggered in this manner may precisely cleave the DNA sequence containing modified -UU- bases on the electrode surface . Thus, the ferrocene (Fc) is changed away from the electrode surface at one end of the DNA , enabling for the recovery of the Electrochemiluminescence (ECL) signal from the two-dimensional material transformed on the electrode surface. As a result, MMP-2 activity was detected utilizing this technique of inhibiting crRNA activity with a detection limit of 15.56 fM. Our suggested method of inhibiting CRISPR/Cas13a activity via PNA has a broad variety of potential therapeutic applications in gene editing and the development of CRISPR/Cas13a-based biosensors.
研究领域:
分子生物学
·
基因编辑
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生物传感技术
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Huiping Chen,Hui Zhang,Miaohui Zhuang,Chen Yang,Kangyun Bao,Yang Li,Shanwen Hu
Spatial segregation via physically isolated units eliminated signal crosstalk. • Programmable scalable strategy enables broad adaptability for multiple pathogens. • Cascaded CHA and HCR achieved dual-signal amplification for higher sensitivity. • Screen-printed electrode arrays enable portable, rapid and stable detection. Seafood is a vital component of the global diet, yet it is highly susceptible to contamination by diverse pathogenic microorganisms during production and distribution, posing significant threats to public health. Herein, we report a novel dual-mode sensing array based on screen-printed electrodes (SPEs) for the simultaneous and rapid detection of multiple pathogenic bacteria in aquatic products via synergistic electrochemiluminescence (ECL) and colorimetric readouts. The spatially separated architecture, comprising distinct recognition, ECL, and colorimetric units, effectively circumvents signal interference. A target transduction system is established to recognize different targets and release a universal transducer strand B for following reactions. Upon target binding, AuNPs-H2/GT probes initiate a catalytic hairpin assembly (CHA) cascade, results in a dual function: it sequesters AuNPs-Ru(bpy) 3 2+ -labeled H1 probes to generate ECL signal, while concurrently releasing GT strands; triggers a hybridization chain reaction with G-quadruplex (G4)-forming hairpins in the separate colorimetric zone, yielding a vivid colorimetric response. By integrating the sensitivity of ECL with the broad linear dynamic range of colorimetry, and utilizing spatial separation to ensure specificity in real samples, this work presents an integrated, cost-effective, and portable platform. It provides a powerful tool for the rapid, visualized, and reliable monitoring of microbial hazards in seafood safety control. Spatially Decoupled Dual-Mode Array for ECL-Colorimetric Detection of Seafood Pathogens via Catalytic Hairpin Assembly and G-Quadruplex. Download: Download high-res image (97KB) Download: Download full-size image
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DOI
高影响因子
ACS Sensors
·
2021
·
IF 7.711
Guangyan Liu,Jun Ling,Jianping Li
A molecularly imprinted electrochemiluminescence sensor was prepared for sensitive and selective determination of aminotriazole via a novel strategy of multiple Ru(bpy)3Cl2 probes released from liposomes immobilized by a light-triggered click reaction. This sensing strategy provides a platform for trace detection of amino-containing pesticides. The target on the molecularly imprinted membrane connected to the Ru(bpy)3Cl2-encapsulated liposomes via the click reaction. After the destabilizing agent Triton X-100 was added, numerous Ru(bpy)3Cl2 molecules were released by liposomes on the molecularly imprinted polymer electrode. The ECL response of the sensor was linearly proportional to the logarithm of the aminotriazole concentration ranging from 5.00 × 10–18 to 1.00 × 10–12 mol/L, and the detection limit was 1.15 × 10–18 mol/L. The sensitivity of the detection was significantly improved, and the analysis process was simplified.
研究领域:
分析化学
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生物传感技术
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Qingqing Zhang,Tingting Hao,Dandan Hu,Zhiyong Guo,Sui Wang,Yufang Hu
A new RNA aptamer-involved electrochemiluminescence (ECL) strategy for sensitive detection of histone acetyltransferase (HAT p300) is designed well based on coenzyme A (CoA)-initiated competitive response and advanced DNA nano-biotechnology. For the first time, this interesting analytical process is comprised of significant histone acetylation catalyzed by HAT p300, competitive replacement triggered by the specific binding of CoA and its RNA aptamer , stable scaffold guided by DNA nano-prism and typical amplification managed by hybrid chain reaction (HCR). Upon recognition of the RNA aptamer to CoA generated in acetylation, auxiliary DNA (A-DNA) which can be captured by three hanging arms of the fabricated DNA nano-prism immobilized onto Au electrode surface is released from the aptamer/A-DNA complex. Moreover, A-DNA can also open skillfully one hairpin DNA (H1), then turning into HCR process by alternating hybridization with the other hairpin DNA (H2). Abundant Ru(phen) 3 2+ molecules which have a strong interaction with dsDNA can result in a significant output. This proposed platform exhibits excellent specificity and sensitivity toward HAT p300 with a detection limit of 0.001 nM and takes on outstanding practicality and superiority, which presents a great potential for HAT p300 analysis in biomedical sciences.
研究领域:
分析化学
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生物化学
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分子生物学
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生物医学科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jing Wen,Lijun Zhou,Ding Jiang,Xueling Shan,Wenchang Wang,Hiroshi Shiigi,Zhidong Chen
Herein, a sensitive and selective electrochemiluminescence (ECL) aptasensor was designed using [email protected] as accelerator towards the perylene derivative (PTC-Cys)/peroxydisulfate (S 2 O 8 2− ) system for kanamycin (KAN) assay. Firstly, the PTC-Cys was prepared by covalently binding l -cysteine to 3,4,9,10-perylenete-tracarboxylic acid, which was acted as the luminophore. Then [email protected] play the part of effective catalyst to accelerate the electrochemical reduction process of S 2 O 8 2− to produce more sulfate radical anions (SO 4 •- ), thus the ECL signal of the compound was noticeably raised by 2.4 times in comparison with that in which only luminophore and S 2 O 8 2− are present, achieving signal amplification of the ECL system. In the presence of KAN, aptamer was pulled down from the sensing interface, achieving a considerable enhancement of ECL intensity in S 2 O 8 2− solution. Upon the optimal condition, our proposed strategy can quantify the concentration of KAN from 1.0 × 10 −13 to 1.0 × 10 −8 M with low limit of detection of 4.2 × 10 −14 M (S/N = 3).Besides, our proposed ECL aptasensor exhibited excellent sensitivity, stability and specificity, and could be successfully applied to detect KAN in practical samples, which proved its potential to detect other antibiotics in food security.
研究领域:
分析化学
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生物传感
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食品安全
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2089-2095
·
IF 4.58
Dong YongPing,Peng Ying,Wang Jiao,Wang ChengMing
Carbon dots (C-dots) display strong cathodic electrogenerated chemiluminescence (ECL) in neutral aqueous solution in the presence of potassium persulfate. ECL intensities strongly depend on the method of synthesis of C-dots. The reactions between the electrochemically reduced intermediates of C-dots and potassium persulfate generate a strong ECL signal. Spectral and electrochemical studies revealed that cytochrome c reacts with potassium persulfate before its electrochemical reduction to generate the strong oxidant radical SO 4
•- which reacts with C-dots to give an enhanced ECL signal. This finding was exploited to design a sensitive ECL assay for cytochrome c. The effects of the amount of C-dots, the pH value, and the persulfate concentration were optimized. Under the optimal conditions, the ECL signals (generated at −1.4 V) increase linearly with the concentration of cytochrome c in the range from 1.0 nM to 10 μM, with a detection limit of 0.2 nM. The modified electrode exhibits high sensitivity and good selectivity.
Carbon dots display strong cathodic electrogenerated chemiluminescence (ECL) in neutral condition with potassium persulfate as coreactant. Cytochrome c strongly enhances the ECL signal, and can be sensitively detected by exploiting this effect.
研究领域:
分析化学
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电化学
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纳米技术
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生物化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Xiaojiao Du,Jun Sun,Yan Li,Wenhan Du,Ding Jiang
A highly sensitive electrochemiluminescence (ECL) aptasensor for lincomycin (Lin) detection was designed based on a novel self-accelerated ECL luminophore of Ag 3 PO 4 -Ti 3 C 2 nanohybrids. The Ag 3 PO 4 -Ti 3 C 2 nanohybrids demonstrated prominent ECL performances with K 2 S 2 O 8 as co-reactant, and the Ti 3 C 2 as co-reaction accelerator, which catalyzes K 2 S 2 O 8 into more active radicals and inducing a tremendous amount of excited-state luminophores. Besides, it can be used for loading aptamer molecules owing to its large surface area. Given these attributes, a self-accelerated ECL aptasensor for Lin detection was constructed based on the effect of target induced aptamer dissociating. Under optimized conditions, the ECL aptasensor exhibited wide linear relationship of 1.0 × 10 −4 nmol/L to 1.0 × 10 2 nmol/L, low detection limit of 6.17 × 10 −5 nmol/L (S/N = 3) and good stability and reproducibility for Lin detection. The aptasensor was successfully applied for the Lin detection in milk and water samples, implying immense potential in real applications.
研究领域:
分析化学
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生物传感器
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纳米技术
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药物分析
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2024
·
IF 4.1
Yahui Zhang,Jing Wang,Yongping Dong
In the present work, the nanosheets of iridium complex (Ir NS) were synthesized by surfactant assisted reprecipitation method. Strong anodic ECL signal of Ir NS was obtained under neutral aqueous condition with electrogenerated hydroxyl radical as coreactant, without the need of additional coreactant. Excellent light absorption ability of gold nanoparticles decorated Cu 2 O cubic nanoparticles (Cu 2 O@Au) could apparently inhibit the ECL intensity of Ir NS. When Cu 2 O@Au was assembled onto the electrode surface by base pair complementarity of DNA, the ECL resonance energy transfer (ECL-RET) system could be fabricated with Ir NS ECL as energy donor and Cu 2 O@Au as energy acceptor. The specific interaction of lincomycin (LIN) with the aptamer could release Cu 2 O@Au from the electrode surface, resulting in the recovery of ECL signal. Based on this, an “off-on” mode ECL sensor was designed to detect LIN. Under the optimal condition, the ECL intensity changed linearly with the logarithm of LIN concentration in the range of 1.0 × 10 −11 –1.0 × 10 −6 mol L −1 with a detection limit of 2.88 × 10 −13 mol L −1 . Importantly, the proposed ECL sensor showed outstanding accuracy and specificity in the detection of LIN, and provided a potential strategy for the determination of antibiotic.
研究领域:
分析化学
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生物传感器
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纳米技术
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DOI
高影响因子
ELECTROANALYSIS
·
2019
·Vol. 32
·期 3
·462-469
·
IF 2.691
Xiaomeng Shan,Tao Pan,Yuting Pan,Wenchang Wang,Xiaohui Chen,Xueling Shan,Zhidong Chen
In this study, a novel electrochemiluminescence (ECL) sensor for highly sensitive and selective detection of Pb(II) was developed based on Ru(bpy) 3 2+ encapsulated UiO66 metal-organic-framework (Ru(bpy) 3 2+ −UiO66 MOF) and −NH 2 group functionalized silica (NH 2 −SiO 2 ). The NH 2 −SiO 2 with large surface area provided an excellent platform for the ECL sensor. As numerous exposed carboxyl groups were present on UiO66 backbone, the Ru(bpy) 3 2+ −UiO66 could be steadily immobilized to NH 2 −SiO 2 by forming amide bonds. Meanwhile, the introduced UiO66 MOF which used for the encapsulation of Ru(bpy) 3 2+ , significantly enhanced the ECL efficiency of the proposed sensor, as it possessed a large specific surface area and porosity for the loading of Ru(bpy) 3 2+ . Moreover, a high quenching effect on ECL intensity was obtained in the presence of Pb(II) in the electrolyte. Under the optimal conditions, the quenched ECL intensity showed a good linear relationship within Pb(II) concentration in the range from 1.0×10 −6 to 1.0×10 2 μM, with a detection limit of 1.0×10 −7 μM ( S / N =3). The proposed sensor for Pb(II) detection was simple in operation, rapid in testing, stable in signal, and showed a good anti-interference ability to some other metal ions. Besides, its application for detecting Pb(II) in a real sample was also investigated here. This work provides a potential platform for metal ions detection in environmental monitoring field.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huiling Li,Li Tian,Shuning Yang,Chao Li,Yujia Song,Ruidan Li,Yanjia Guo,Juan Lu
A signal-on solid-state electrochemiluminescence (ECL) sensor based on luminol@carbon nanotubes (luminol@CNTs) and CdTe-ZnS@hydroxyapatite (CdTe-ZnS@HAP) was constructed for the detection of paclitaxel (PTX). In this sensing platform, luminol was used as the main luminophore and CdTe-ZnS was used as a co-reaction accelerator. CNTs and HAP were acted as carriers to adsorb more luminophores and co-reaction promoters. In addition, due to the enhanced effect of PTX on the ECL intensity of the luminol-O 2 system, PTX was introduced as the final detection target. Under the optimal experimental conditions, the ECL intensity change values (ΔI) of the sensor showed a good linear relationship with the logarithm of PTX concentration in the range of 3 × 10 -12 − 3 × 10 -7 molL -1 , and the limit of detection was 1 × 10 -12 mol L -1 (S/N = 3). This method was successfully applied to the determination of trace PTX concentration in serum.
研究领域:
分析化学
·
药物检测
·
纳米材料科学
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DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Yixin Liu,Binxiao Li,Yuanyuan Yao,Beibei Yang,Tongtong Tian,Yan Miao,Baohong Liu
An electrochemiluminescence (ECL) resonance energy transfer system is constructed for 17β-estradiol (E2) detection using α [email protected] nanospheres as the ECL-active substrates and Ag NCs as an efficient quencher. CdS QDs loaded onto three-dimensional (3D) urchin-like α-FeOOH nanospheres (α [email protected] nanospheres) exhibited excellent ECL responses, which is attributed to dual-amplification of α-FeOOH frameworks. The 3D hierarchical structure of the α-FeOOH nanospheres provided abundant sites for loading ECL-active species, thus significantly improving the ECL performance of substrates; While Fe 3+ presented on surface of α-FeOOH nanospheres could be reduced to Fe 2+ in negative potentials, after which might activate persulfate in a Fenton-like process, resulting in more sulfate free radicals for more effective ECL responses via electron transfer reactions. Additionally, Ag nanoclusters (Ag NCs) stabilized by single stranded oligonucleotide were introduced as quenching probes for CdS QDs owing to the well-matched donor-acceptor spectrum for efficient energy transfer, which makes them appropriate for detection of E2. The proposed strategy displayed a desirable dynamic range from 0.01 to 10 pg mL −1 with a limit of detection of 0.003 pg mL −1 . The proposed strategy based on the ECL-RET strategy offered an ideal way for E2 detection, and also revealed an alternative platform for detection of other small molecules.
研究领域:
分析化学
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生物化学
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纳米技术
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环境科学
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DOI
PubMed
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 5
·291
·
IF 5.6
Yawen Zhu,Xuemei Wang,Zhiyong Yan,Feifei Zhang,Jianfei Xia,Lili Lv,Zonghua Wang
The dual-signal output self-calibration mode reduces the false positive and negative signals of electrochemiluminescence (ECL) aptamer sensors. A competitive dual-signal ECL platform was designed for the ultrasensitive detection of kanamycin (KAN) using a zirconium metal–organic framework (Zr MOF) and Luminol as ECL emitters. To enhance the ECL efficiency, a co-reactant (polyethyleneimine, PEI) was covalently bound to the Zr MOF to achieve self-enhanced ECL. Based on the selective interaction between KAN and its aptamer, the Luminol/KAN/Zr MOF-PEI “sandwich” structure was immobilized on the electrode surface. The competition for PEI between emitters increased the Luminol ECL signal and decreased the Zr MOF’s ECL signal. The ratio in ECL signals between the two competitive emitters enabled the quantitative analysis of KAN, achieving a detection limit as low as 7.86 × 10−4ng/mL. This study elucidated the synergistic mechanism between self-enhanced ECL and ECL competition, offering a novel approach for constructing dual-signal ECL sensors using a single co-reactant.
研究领域:
分析化学
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生物传感
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）