Analytical Methods
·
2026
·
IF 3
Xianggang Yin,Xiaohan Zhao,Xin Chen,Jiayi Jiang,Lele Li,Qinting Jiang,Linlin Wu,Yifeng Zhou,Jun Huang
A digital image colourimetry (DIC) method was developed to rapidly determine the acid value (AV), peroxide value (PV) and iodine value (IV) of fish oils. The copper-soap, iodometric, and Wijs reactions were adapted for smartphone imaging by performing the reactions in light-isolated boxes and setting the acquisition settings to ensure consistent colour capture. Colour features from RGB/Lab/YUV spaces were extracted and screened using the Pearson correlation coefficient to retain only the most informative channels for AV, PV and IV. Six regression algorithms were compared, and the random forest model showed the highest predictive accuracy. The method exhibited acceptable repeatability and low detection limits, and it agreed closely with titration results, with a correlation coefficient above 0.99. Relative to titration, the DIC approach reduced reagent consumption by at least 94%, shortened the analysis time per sample by at least 84%, and reduced the required sample mass to 0.02–1.0 g. It also avoided the use of ether and chloroform employed in some conventional titration procedures, although cyclohexane, isooctane, and glacial acetic acid were still required. DIC thus provides a rapid, low-cost, and more resource-efficient alternative for routine screening and quality control of fish oil oxidation.
✓ 已验证
DOI
PubMed
高影响因子
npj Science of Food
·
2026
·
IF 9.7
Chen Yu,Pan Yi,Hu Yu-Ting,Yang Le-Ying,Wang Lin-Ru,Sha Xiao-Mei,Tu Zong-Cai
Neutral protease and gum arabic (GA) were combined to enhance foaming ability (FA) and foaming stability (FS) of fish gelatin. Meanwhile, structural changes of pure, single-modified and combined-modified fish gelatins were evaluated in detail to investigate the mechanism. FA and FS of combined-modified fish gelatin were boosted to the maximum of 158% and 55%, respectively. The enzymolysis enhanced the surface hydrophobicity (increased by 40%) and destroyed the microstructure. On the basis, hydrogen bond and electrostatic forces of combined-modified fish gelatin were strengthened by GA, which elevated viscosity and interfacial protein adsorption content (6 mg/g), enhancing FS and FA. The redshift of amide I and II wavenumbers indicated more stable secondary structure of combined-modified fish gelatin, reflected in the SEM-verified denser foam. The underlying mechanism for improving foaming properties of fish gelatin was related to the opened structure by neutral protease and further noncovalent interaction by GA. New insights for the development of sustainable foaming agents were provided by these findings.
✓ 已验证
产品关联
DOI
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-12
·
IF 7.8
Chen Lijia,Zhang Wenjie,Bian Qiqi,Wang Xichang,Zhong Jian
The application of fish oil is limited by its unpleasant fishy flavour, sensitivity to environments (e.g., oxygen, light, and heat), and poor water solubility. The complex coacervation-based encapsulation of fish oil in powders is a promising method to improve the behavior of fish oil in food science and engineering. Controlled preparation of fish oil powders has become an important topic in improving their properties. Herein, the fish oil-encapsulated powders were controlled prepared by combining a fish gelatin (FG)-alginate complex coacervation method with an intra-particulate genipin-crosslinking method. The genipin crosslinking did not affect the particle morphologies, moisture contents, and fish oil encapsulation ability. However, it did affect other physicochemical properties, fish oil oxidation ability, and in vitro digestion behaviors. Especially, the genipin-to-FG mass ratio of 1.0% induced the lowest water activity (0.45 ± 0.01), highest tapped density (0.107 ± 0.007 g/cm 3 ), highest Carr indexes (58% ± 2%), highest Hausner ratios (2.43 ± 0.13), lowest peak value (636.0 ± 10.0 μm) of the powder sizes, and lowest peroxide peak value (442 ± 3 mmol/kg oil). This work provided useful information to understand the relationship between genipin modification and the properties of the fish oil-encapsulated powders.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhaopeng Li,Pei Li,Jinfu Zhang,Helei Yang,Zhiguo Zhu,Hua Zhou,Xia Sun,Zhenhe Wang
This study reports on the development of a NiO quantum dot-modified ZnO gas sensor, which is designed for detecting ethyl formate, a crucial indicator of fish meal spoilage. The results indicated that NiO quantum dots were uniformly dispersed on the surface of ZnO, leading to the formation of a P N heterojunction that enhanced the gas sensing properties. Among the sensors tested, the S2 sensor (with a NiO doping of 0.1 mmol) exhibited the highest response value of 15.75 when exposed to 100 ppm of ethyl formate at 260 °C, additionally presented out excellent selectivity, linear response behavior, and long-term stability. The superior performance of the S2 sensor can be attributed to its unique jackfruit-like structure and the formation of P N junctions. These features increase the number of active sites and modulate the electronic properties of the material. The S2 sensor has been implemented for the assessment of fish meal freshness. This study emphasizes the great potential of this sensor for the real-time monitoring of fish meal freshness, offering significant applications in the field of food and feed safety.
研究领域:
材料科学
·
纳米技术
·
食品安全
·
化学传感
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 11
·902
·
IF 5.3
Xi Zheng,Xin Feng,Yue Huang,Tao Zeng
In this study, the complexes (FG-P) based on fish gelatin (FG) and pectin (P) were prepared by a simple physical blending within a range of pectin concentrations (0–2%,w/v). The structure, interface, and emulsification properties of the obtained FG-P were analyzed. The binding between FG and pectin was dominated by electrostatic interaction and hydrogen bonding. Introducing pectin substantially increased the viscosity of FG-P. The water contact angle of FG-P gradually decreased with increasing pectin concentration. The highly interfacial viscosity and hydrophilicity of FG-P hindered the interfacial adsorption at the oil/water phase, thereby increasing the interfacial tension and phase angle. This was further manifested as an increase in the viscous modulus and a decrease in both the total modulus and elastic modulus. Despite the inhibition of interfacial adsorption, the unabsorbed FG-P was uniformly dispersed in the continuous phase to form a compact network structure, accompanied with improved rheological properties. Correspondingly, the emulsion precipitation phenomenon was effectively inhibited, and the stability of FG-P stabilized emulsions was improved with decreased droplet size.
研究领域:
食品科学
·
胶体与界面化学
·
高分子科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Jin Feng,He Cai,Hua Wang,Chunyang Li,Songbai Liu
Conjugates of ovalbumin (OVA) with (+)-catechin (C), (−)-epigallocatechin (EGC) or (−)-epigallocatechin gallate (EGCG) were synthesized through a free-radical grafting approach. The covalent binding between OVA and catechins was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). OVA-catechin conjugates were further characterized by a combination of steady-state fluorescence spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy and circular dichroism (CD) analyses. Results suggested that the conjugates contained less α-helix but more β-sheet secondary structure than OVA. The catechin-grafting reaction led to improved surface hydrophobicity and decreased tertiary conformation stability of OVA. Compared with that emulsified by OVA, fish oil emulsion coated by conjugates demonstrated smaller droplet size, better storage stability and less viscosity. Besides, conjugates inhibited the lipid oxidation in fish oil emulsion more effectively than OVA because of their higher antioxidant activity and interfacial accumulation.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Lu Zeng,Xinxin Xu,Lingling Guo,Zhongxing Wang,Hongliu Ding,Shanshan Song,Liguang Xu,Hua Kuang,Liqiang Liu,Chuanlai Xu
To ensure food quality and prevent histamine (HA) toxicity, a rapid and direct method of detecting HA is required. In this work, we prepared a monoclonal antibody (mAb) against HA using a hapten produced by the introduction of a phenyl-containing linker. The novel mAb exhibited high sensitivity against HA as determined by ELISA, with a half-maximal inhibitory concentration of 21.51 ng/mL. A gold nanoparticle-based immunosensor was fabricated for rapid detection of HA in fish samples. After optimizing the immunosensor, a visual limit of detection (LOD) and a calculated LOD were 0.25 mg/kg and 10.48 μg/kg for HA, respectively. Recovery rates from the spiked fish samples ranged from 87.33% to 104.67% with the coefficient of variation below 10.82%. Concurrently, the whole process in testing real sample was completed within 15 min, and all results were well confirmed and comparable by liquid chromatography-mass spectrometry and the commercial test strip. These data revealed that the proposed immunosensor could be used as a monitoring tool for the rapid and direct detection of HA in fish samples.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
Food and Bioprocess Technology
·
2024
·Vol. 17
·期 11
·3693-3703
·
IF 5.3
Xu Chengzhi,Cai Chaonan,Liu Tianyi,Kang Jizhen,Li Sheng,Zhang Juntao,Wei Benmei,Wang Haibo
An important area of focus for developing antioxidant collagen hydrolysates (peptides) involves enhancing the antioxidant properties of collagen hydrolysates. In this study, fish scale collagen hydrolysate (FH) was used as the raw material for plastein reaction. Various enzymes, including papain, alcalase, flavourzyme, and the combination of alcalase and flavourzyme, were employed for this purpose. The plastein reaction significantly improved the thermal stability, chemical antioxidant activity, and capacity to scavenge cellular reactive oxygen species of FH. Notably, the plastein reaction catalyzed by alcalase exhibited the most significant improvement, increasing the hydroxyl radical scavenging rate from 72.3 to 93.4% and restoring the viability of the oxidative stress-induced HepG2 cell model from 50.8 ± 1.7 to 74.9 ± 1.7%. During the plastein reaction, condensation and hydrolysis reactions occurred simultaneously, with condensation being the dominant process. These reactions, along with physical aggregation, facilitated the formation of larger yet more concentrated collagen peptide aggregates, leading to increased exposure of hydrophobic groups. This enhanced the uptake of collagen hydrolysates by the cells and contributed to the enhancement of their antioxidant properties. Thus, the plastein reaction is an effective method for enhancing the antioxidant properties of collagen hydrolysates, with its simplicity of operation and promising application potential.
研究领域:
食品科学
·
生物化学
·
生物技术
·
海洋生物技术
·
药学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Wei Chen,Zhenhe Wang,Shuang Gu,Jun Wang,Yongwei Wang,Zhenbo Wei
Some aldehydes (hexanal, octanal , and nonanal) are specific volatile organic compounds (VOCs) related to the quality of both freshwater fish and marine fish products, and these fish products are usually with relative high moisture. Herein, a quartz crystal microbalance (QCM) gas sensor modified with the hydrophobic amino-functionalized graphene oxide (AGO) nanocomposite for aldehydes detection was reported. The as-prepared materials were studied by X ray diffraction (XRD) patterns, Fourier transform infrared (FTIR) spectra and X-ray photoelectron spectroscopy (XPS). The sensor was exposed towards air streams containing aldehydes (hexanal, octanal, and nonanal in single, binary, and tertiary mixture) vapors with different concentrations under 80 % relative humidity (RH), and the sensor responded well towards aldehydes within 45 ppm. The sensor characteristics were validated by hydrophobicity, sensitivity, reversibility, selectivity and stability analyses. The sensing mechanism was proposed as (1) resistance to water vapor by hydrophobic silica nanoparticles; (2) hydrogen bonding interactions between the functional groups of AGO and aldehydes based on Gaussian calculations. This sensor was successfully applied to detect aldehydes in grass carp fish fillets and hairtail fillets. Solid phase micro extraction-gas chromatography mass spectrometer (SPME-GCMS) was used to detect aldehydes in the same fillets samples, and the results of the sensor and SPME-GCMS were high related with a correlation coefficient of 0.98.
研究领域:
食品科学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Zhonglin Cao,Tiansheng Gan,Gaixia Xu,Chunfeng Ma
Inspired by fish skin, biomimetic self-renewal poly[(ethylene oxide)-co-(ethylene carbonate)] (PEOC) brushes with protein resistance had been prepared via surface-initiated ring-opening polymerization (ROP). The results of hydrolytic degradation indicated that the PEOC brushes could degrade in artificial seawater. Ellipsometry, X-ray photoelectron spectrometry, and contact angle results demonstrated that the PEOC brushes degrade uniformly. By using a quartz crystal microbalance with dissipation, we studied the protein adsorption on the surfaces in artificial seawater at different degradation times. After 24, 48, 96, and 168 h of degradation, the PEOC surfaces showed nearly zero Δf and ΔD for bovine serum albumin, lysozyme, and fibrinogen. More importantly, there was a notably lower density of microorganisms adhered to the surface modified with PEOC compared with that of the surface without PEOC in natural seawater. The current study showed that the PEOC brushes exhibit a self-renewal property with persistent protein resistance and prevent the adhesion of microorganisms. Such a biomimetic polymer had a great potential in marine antibiofouling.
研究领域:
材料科学
·
生物医学工程
·
海洋科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Bo Zhang,Lei Zhang,Lujue He,Xiaodong Yang,Yali Shi,Shaowei Liao,Shan Yang,Jiagao Cheng,Tianrui Ren
Fipronil is an efficient phenylpyrazole insecticide that acts on insect γ-aminobutyric acid (GABA) receptors (GABARs) and has low toxicity to mammals but high toxicity to nontarget organisms such as fish. To develop novel efficient low-toxicity insecticides, it is necessary to determine the detailed toxic mechanism at the molecular target level. In this work, methods including affinity chromatography, fluorescent-labeled binding assays, and molecular modeling were integrated to explore the binding of fipronil to GABARs in fish (Aristichthys nobilis) and insects (Musca domestica). Affinity chromatography revealed that fipronil acts on two different subunits of GABARs in fish and M. domestica. Moreover, fluorescence assays revealed that fipronil exhibits similar affinity to the two GABARs. The Kd and Bmax of fipronil binding to the A. nobilis GABAR were 346 ± 6 nmol/L and 40.6 ± 3.5 pmol/mg of protein, respectively. And the Kd and Bmax of fipronil binding to the GABAR in M. domestica brain were 109 ± 9 nM and 21.3 ± 2.5 pmol/mg of protein, respectively. In addition, similar fipronil binding positions but different binding modes were observed in docking studies with Brachydanio rerio var. and M. domestica GABARs. These findings indicated similar interactions of fipronil with fish and insects, leading to high toxicity. The different binding features of fipronil between the two species might be helpful for the design and development of highly selective insecticides with low toxicity to fish.
研究领域:
环境毒理学
·
分子生物学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2023
·Vol. 10
·期 12
·3389-3400
·
IF 7.3
Shuoli Ma,Wen-Xiong Wang
Marine hydrosphere is in grave danger if extreme weather conditions, such as high temperatures and heat waves, become the norm. The functional characteristics of nano Zn oxides (nZnO) might provide a “booster substance” that helps marine species to endure environmental stress. In the present study, physiological indicators and simulated severe environments were used to evaluate the antioxidative ability of nZnO in marine rabbitfish Siganus fuscescens, with an aim to help them resist extreme environmental stress. Rabbitfish were exposed to different diets with ZnSO4 and nZnO at the optimum requirement level (Zn content of 62.5 μg g−1), and then challenged with a low dissolved oxygen concentration and higher temperature. We demonstrated that dietary nZnO promoted the anti-oxidation defense system of the rabbitfish by improving the activities of the antioxidant enzymes, i.e., catalase (CAT), superoxide dismutase (SOD), and the reduced glutathione/oxidized glutathione (GSH/GSSG) ratio without tissue damage. Compound stressors reduced the median survival duration of the rabbitfish from 4.25 h to 2.5 h (dietary exposure) and 9 h to 5.75 h (dietary nZnO exposure) compared to single environmental stress. nZnO downregulated the HIF gene expression and increased the prolyl hydroxylase (PHD) activity in the liver of the rabbitfish. After 10 h of extreme environmental exposure, the gene expression results indicated that nZnO inhibited the NF-κB/IL-1β/IL-6 pathway and TNF-α/Bcl-2/Bax/caspase 9 pathway and activated the akt/mTOR/elovl5 and elovl8 pathway of the rabbitfish to maintain the resilience of the fish under the extreme environment. These results strongly suggested that nZnO may improve the anti-oxidation ability of fish to resist extreme environments.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）