SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Tarun Kumar Patle,Kamlesh Shrivas,Ramsingh Kurrey,Seema Upadhyay,Rajendra Jangde,Ravishankar Chauhan
Here, we report an ultrasonic-assisted extraction (UAE) of phytochemicals from bark, leaves, sepals, fruits, and seeds of Dillenia pentagyna (Roxb) using different organic solvents such as chloroform, ethanol, and n-hexane. The preliminary phytochemical screening results showed that the ethanolic extract is enriched with phenolics, flavonoids , tannin , saponin , alkaloid, and terpenoids . The profiling of phytochemicals is carried out employing UV–Vis and Fourier-transform infrared (FTIR) spectroscopy analyses. The higher amount of phenolic compounds obtained in the ethanolic extract of bark and leaves as compared to other parts of the plant. Consequently, a higher amount of total flavonoid compounds unveiled in the bark of targeted species. The ethanolic extract of bark and leaves showed good free radical scavenging activity using DPPH with inhibition percentage of 90.58 ± 1.89% and 76.46 ± 1.58%, respectively, in comparison to standard ascorbic acid at 10 μg/mL. Moreover, the half-maximal inhibitory concentration (IC 50 ) value of bark and leaves are 5.64 and 6.54 μg/mL, respectively, in comparison to standard ascorbic acid . With the best of our knowledge, it is the first report pertaining to characterization and quantification of phenols and flavonoids as well as the investigation of the medicinal property in D. pentagyna .
研究领域:
植物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Gaoqiang Lv,Didi Shan,Yongge Ma,Wenya Zhang,Duobujie Ciren,Shuo Jiang,Bojun Dang,Jiashun Zhang,Weihong Sun,Hanping Mao
Pesticide residues on plant surfaces pose a severe threat to food security, yet most research has focused on monitoring the liquid matrix, with few reports conducting in-situ analysis of the residues. This study was the first to attempt to utilize portable attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) for in-situ characterization of broad-spectrum fungicide boscalid residues on plant surfaces. ATR-FTIR scanning of tomato fruits with pre-determined concentrations of boscalid residues was conducted without any pre-treatment, and the obtained spectra were then processed using chemometrics methods. The results demonstrated a negative correlation between the residual concentrations and their corresponding absorption intensities of several well-resolved peaks from the spectra, resulting in a high accuracy of 93.33% for the classification model created by probabilistic neural network (PNN) coupled with principal component analysis (PCA). By employing correlation analysis and the interval partial least squares method (iPLS), quantitative analysis was conducted on the wavenumber ranges of 1000–1800 cm −1 and 2700–2900 cm −1 from the spectra. The regression model, established through partial least squares regression (PLSR), demonstrated exceptional performance with an R 2 value of 0.80, RMSE of 1.02 μg/cm 2 , RPD of 2.0, and RPIQ of 2.1 for validation. Meanwhile, the detection limit (LOD) of the model was calculated as 3.06 μg/cm 2 . This report highlights the potential of using portable ATR-FTIR for conducting qualitative and quantitative monitoring of pesticide residues both in-situ and on-site. It also provides references for other measuring techniques.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Jiawei Zhu,Wenting Ren,Fei Guo,Hankun Wang,Yan Yu
Understanding the distribution and accessibility of polymers within plant cell walls is crucial for addressing biomass recalcitrance in lignocellulosic materials. In this work, Imaging Fourier Transform Infrared (FTIR) and Raman spectroscopy , coupled with targeted chemical treatments, were employed to investigate cell wall polymer distribution in two bamboo species at both tissue and cell wall levels. Tissue-level Imaging FTIR revealed significant disparities in the distribution and chemical activity of cell wall polymers between the fibrous sheath and fibrous strand. At the cell wall level, Imaging Raman spectroscopy delineated a distinct difference between the secondary wall and intercellular layer, with the latter containing higher levels of lignin, hydroxycinnamic acid (HCA), and xylan , and lower cellulose. Mild acidified sodium chlorite treatment led to partial removal of lignin, HCA, and xylan from the intercellular layer, albeit to a lesser extent than alkaline treatment, indicating susceptibility of these polymers to chemical treatment. In contrast, lignin in the secondary wall exhibited limited reactivity to acidified sodium chlorite but was slightly removed by alkaline treatment, suggesting stable chemical properties with slight alkaline intolerance. These findings provide valuable insights into the inherent design mechanism of plant cells and their efficient utilization.
研究领域:
植物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2021
·
IF 1.134
Xiao-Xuan ZHANG,Wu-Bo CHU,Nan JIANG,He LI
Serum albumin is the most abundant protein in mammalian plasma and is often used as a model to understand the adhesion behavior of proteins on surfaces. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is used to non-destructively and dynamically detect the chemical change information between the protein and the surface during the adhesion process, which is especially suitable for in-situ measurement of samples in water. Therefore, the adhesion of bovine serum albumin (BSA) on the surface in H 2 O and D 2 O is investigated by ATR-FTIR technology, which effectively avoids the interference of the characteristic peak of deformation vibration of liquid H 2 O (1645 cm −1 ) on the amide I band of BSA. Sodium alginate, a polysaccharide widely present in the ocean, is introduced into the BSA solution to explore the changes in the adhesion process of proteins on the surface of ZnSe, and polarized ATR-FTIR spectra are also collected to study the adhesion orientation of BSA. Sodium alginate presents a negative effect on the adhesion of BSA on the surface and there is no specific orientation of BSA on the surface.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 3.473
Lei Wang,Wen Liu,Ting Wang,Jinren Ni
For highly efficient removal of Cr(VI) from aqueous solutions, amino-functionalized titanate nanotubes (NH 2 -TNTs) with excellent adsorption performance have been synthesized by covalently grafting [1-(2-amino-ethyl)-3-aminopropyl]trimethoxysilane (AAPTS) onto protonated titanate nanotubes (HTNTs) with great amounts of surface hydroxyl groups. TEM and XRD results confirmed that the nanotubular morphology and crystal structure of HTNTs and NH 2 -TNTs were preserved. FTIR spectra demonstrated that AAPTS was covalently bonded on the surface of HTNTs. Batch adsorption experiments showed that pseudo-second-order kinetics model and Langmuir isotherm model fitted the adsorption data very well for both materials, and the Cr(VI) adsorption capacity on NH 2 -TNTs calculated by Langmuir model was up to 153.85 mg g −1 at initial pH 5.4 and 30 °C, much larger than that on HTNTs (26.60 mg g −1 ). Moreover, uptake of Cr(VI) ions onto NH 2 -TNTs could be completed within only 5 min for 95% adsorption of the maximum. Influence of different species of Cr(VI) under varying pH was also considered. FTIR and XPS analysis indicated that Cr(VI) ions were first exchanged with NO 3 - linked on the positively charged amino groups and then partially reduced to Cr(III). Afterwards, Cr(III) were then totally chelated with amino groups and no Cr(III) was detected in the solution after Cr(VI) adsorption at pH range of 1–12.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Frontiers in Molecular Biosciences
·
2022
·
IF 6.113
Yaguchi Momo,Jia Xiaodan,Schlesinger Ramona,Jiang Xiue,Ataka Kenichi,Heberle Joachim
Direct optical activation of microbial rhodopsins in deep biological tissue suffers from ineffective light delivery because visible light is strongly scattered and absorbed. NIR light has deeper tissue penetration, but NIR-activation requires a transducer that converts NIR light into visible light in proximity to proteins of interest. Lanthanide-doped upconversion nanoparticles (UCNPs) are ideal transducer as they absorb near-infrared (NIR) light and emit visible light. Therefore, UCNP-assisted excitation of microbial rhodopsins with NIR light has been intensively studied by electrophysiology technique. While electrophysiology is a powerful method to test the functional performance of microbial rhodopsins, conformational changes associated with the NIR light illumination in the presence of UCNPs remain poorly understood. Since UCNPs have generally multiple emission peaks at different wavelengths, it is important to reveal if UCNP-generated visible light induces similar structural changes of microbial rhodopsins as conventional visible light illumination does. Here, we synthesize the lanthanide-doped UCNPs that convert NIR light to blue light. Using these NIR-to-blue UCNPs, we monitor the NIR-triggered conformational changes in sensory rhodopsin II from Natronomonas pharaonis (NpSRII), blue light-sensitive microbial rhodospsin, by FTIR spectroscopy. FTIR difference spectrum of NpSRII was recorded under two different excitation conditions: (ⅰ) with conventional blue light, (ⅱ) with UCNP-generated blue light upon NIR excitation. Both spectra display similar spectral features characteristic of the long-lived M photointermediate state during the photocycle of NpSRII. This study demonstrates that NIR-activation of NpSRII mediated by UCNPs takes place in a similar way to direct blue light activation of NpSRII.
研究领域:
生物物理学
·
光学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chongshan Yang,Dandan Duan,Chunwang Dong,Chuanxia Li,Guanglin Li,Yunhai Zhou,Yifan Gu,Yachao Liu,Chunjiang Zhao,Daming Dong
Aroma is a key factor used to evaluate tea quality. Illegal traders usually add essence to expired or substandard tea to improve its aroma so as to gain more profit. Traditional physical and chemical testing methods are time-consuming and costly. Furthermore, rapid detection techniques, such as near-infrared spectroscopy and machine vision, can only be used to detect adulterated powdered solid essences in tea. In this study, proton-transfer reaction mass spectrometry (PTR-MS) and Fourier-transform infrared spectroscopy (FTIR) were employed to detect volatile organic compounds (VOCs) in samples, and rapid detection of different tea adulterated liquid essence was achieved. The prediction accuracies of PTR-MS and FTIR reached over 0.941 and 0.957, respectively, and the minimum detection limits were lower than the actual used values in both. In this study, the different application scenarios of the two technologies are discussed based on their performance characteristics.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongyan Pei,Mengqi Zhang,Xiaoying Wu,Hua Cao
Seaweed polysaccharides exhibit skin-protective properties, yet species-specific structural determinants of their functionalities remain unexplored. Here, polysaccharides from Sargassum, Ulva and Laminaria digitata were extracted via response surface methodology (RSM)-optimized protocols, quantified by HPLC (monosaccharide composition, sulfate content) and characterized by Fourier transform infrared spectroscopy (FTIR), 13 C nuclear magnetic resonance ( 13 C NMR) and gel permeation chromatography (GPC). Functional validation across cell (RAW264.7 macrophages, HaCat and chorioallantoic membrane assays), ex vivo (antioxidant capacity, UV absorption, hygroscopicity) and in vivo models (rat auricle edema, skin irritation) confirms both efficacy and safety for cosmeceutical use. Sargassum contains a dominant 599.87-kDa fucoidan with diagnostic NMR signals at δ15.05 ppm (C6-CH₃ of L-fucose) and δ100.25–101.87 ppm (anomeric C1), alongside FTIR bands for sulfate (1250 cm −1 ), fucose-specific C–O–S bending (840 cm −1 ) and hydration-associated O H bending (1650 cm −1 ), correlating with its antioxidant activity (62.67 ± 5.22 % hemolysis inhibition). Ulva comprises a 22.21-kDa ulvan exhibiting NMR peaks at δ107.05 ppm (C1 of α-L-rhamnose-3-sulfate) and δ16.86 ppm (C6-CH₃ of rhamnose), plus FTIR signatures of sulfate (1240 cm −1 ), broad O H stretching (3400 cm −1 ) and rhamnose C H deformation (890 cm −1 ), enabling hygroscopic hydrogel formation and UV shielding (61.31 ± 0.78 %). Laminaria digitata features a 180.29-kDa alginate with NMR signals at δ176.89 ppm (carboxyl carbon) and δ102.78–103.54 ppm (anomeric carbons of mannuronic and guluronic acid residues), and FTIR bands for carboxyl (1720 cm −1 ) and bound water (1640 cm −1 ), driving moisturization (64.81 ± 10.01 %) and hyaluronidase inhibition (27.26 ± 2.03 %) . All polysaccharides demonstrated zero irritation and excellent biocompatibility, thus supporting species-specific cosmetic applications: Sargassum for antioxidant-focused formulations, Ulva for UV-protective products and Laminaria digitata for hydration and anti-inflammatory-oriented cosmeceuticals.
研究领域:
海洋生物化学
·
化妆品科学
·
天然产物化学
·
光谱分析
·
多糖表征
·
功能生物材料
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE ENERGY INSTITUTE
·
2025
·
IF 6.2
Yueping Liu,Suning Li,Yongcheng Yang,Yongxiang Liang,Fei Wang,Xuetong Li,Deng Zhao,Yanjun Lu,Hui Liu,Shuai Guo
The aim of this study is to investigate the role of different carbon-based structures on the pyrolysis pathway and gas product distribution of polypropylene (PP). Using in situ DRIFTS coupled with thermogravimetric mass spectrometry, this study investigated the synergistic mechanisms between polypropylene (PP) and common plastics and biomass at the molecular level. The following mechanisms were revealed: 1) Polyethylene erephthalate (PET) induces demethylation of PP through oxygenated fragments, which weakens the stability of its main chain and synergistically reduces the pyrolysis temperature. The high-temperature polyene structure of polyvinyl chloride (PVC) promotes further breakage of the PP main chain, synergistically driving the directional conversion of gas products to unsaturated hydrocarbons and chlorine-containing compounds. The phenyl groups in polystyrene (PS) trigger PP activation at low temperatures, improving the yield of toluene gas. 2) PP inhibits the decomposition of blended components through physical entanglement at low temperatures. At high temperatures, polyenes, phenyl groups and other cleavage products reorganize with PP hydroxyl fragments, promoting the enrichment of olefins, aromatics and chlorinated compounds. 3) In PP-biomass systems, it is hypothesized that the PP pyrolysis pathway is altered, enhancing the selectivity for short-chain hydrocarbons and monocyclic aromatics. This effect is likely mediated through ash-catalyzed reactions and induction by oxygen-containing fragments. This study employs in situ Fourier-transform infrared (FTIR) spectroscopy to monitor in real time the molecular-level evolution of PP-based mixed waste plastics and biomass pyrolysis, revealing fundamental carbon-chain structure-property relationships. These findings establish a mechanistic framework for the rational design of high-efficiency pyrolysis systems with precisely controlled product distributions through synergistic interactions.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOILS AND SEDIMENTS
·
2024
·Vol. 24
·期 6
·2309-2317
·
IF 2.8
Mi Na,Yang Min,Wang Xiaohan,Sun Qian,He Jianzhou,Deng Shaopo,Fan Tingting
Purpose The application of silicon could effectively alleviate Cd contamination in the soil. However, the interaction between silicon and Cd in soils is not well understood. Our research aims to investigate the mechanism of the impact of silicate on the sorption of Cd on metal oxides in soils. Materials and methods Sodium silicate and Cd(II) co-sorption on γ-Al 2 O 3 and goethite at pH 5.5 and 7.5 was studied using a combination of batch sorption experiments, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Results and discussion Silicate enhanced the sorption of Cd(II) onto γ-Al 2 O 3 at pH 5.5 and 7.5. However, the sorption of Cd(II) on goethite at pH 5.5 and 7.5 was only promoted when the silicate concentration reached 1 mmol L −1 . Cadmium(II) enhanced silicate sorption on γ-Al 2 O 3 at pH 5.5 and on goethite at pH 7.5, while it decreased silicate sorption on γ-Al 2 O 3 at pH 7.5 and had no significant effect on goethite at pH 5.5. At pH 5.5, Cd(II) reacted with silicate to generate Cd 2 SiO 4 , and Cd-Si-Al ternary complexes were formed on γ-Al 2 O 3 . At pH 7.5, silicate promoted the formation of Cd(OH) 2 on γ-Al 2 O 3 , which ultimately transformed into CdCO 3 . When silicate concentration reached 1 mmol L −1 , more silicate complexes were formed through the Fe-O-Si bound on goethite at both pH 5.5 and 7.5 to promote the adsorption of Cd(II) owing to its electronegative functional group on the surface of the complex. Conclusions The type of clay minerals, pH, and the presence of silicate all significantly affected Cd sorption. Furthermore, the sorption mechanism of Cd on γ-Al 2 O 3 and goethite in the presence of silicate has been elucidated. The above findings improve our understanding of the mechanism for immobilizing Cd in soils using silicon-containing materials.
研究领域:
土壤科学
·
环境化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Jingyang Zhang,Li Kang,Feigang Zhao,Chao Mu,Dan Guo,Shengping Wang,Xinbin Ma
The crystal planes tailored lattice oxygen mechanism for the competitive and promising process of directly synthesizing dimethyl carbonate (DMC) from carbon dioxide (CO 2 ) and methanol have been comprehensively investigated. Steady-state in-situ micro-reaction Fourier transform infrared spectroscopy (FTIR) combined with density functional theory (DFT) calculations were conducted on a series of CeO 2 model catalysts to elucidate the formation pathway of DMC. The previously theoretically predicted methoxycarbonyl (MC, CH 3 OCO*) intermediate was experimentally identified through FTIR spectroscopy. Only on the active (110) surface do lattice oxygen and Ov participate in the reaction cycle, facilitating critical reaction steps and shifting the reaction pathway from the electrophilic addition of methyl species to monomethyl carbonate (MMC, CH 3 OCOO*) on the (111) surface to the nucleophilic addition of methoxy species to MC. The established structure-activity relation holds a promise for providing effective guidance in CeO 2 -based catalyst design at a mechanistic level.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2022
·Vol. 12
·期 12
·1562
·
IF 4.501
Di Liu,Hui-Zi Huang,Zhejiaji Zhu,Jiani Li,Li-Wei Chen,Xiao-Ting Jing,An-Xiang Yin
The development and commercialization of direct ethanol fuel cells requires active and durable electro-catalysts towards the ethanol oxidation reactions (EOR). Rational composition and morphology control of Pt-based alloy nanocrystals can not only enhance their EOR reactivity but also reduce the consumption of precious Pt. Herein, PtCu nanocubes (NCs)/CB enclosed by well-defined (100) facets were prepared by solution synthesis, exhibiting much higher mass activity (4.96 A mgPt−1) than PtCu nanoparticles (NPs)/CB with irregular shapes (3.26 A mgPt−1) and commercial Pt/C (1.67 A mgPt−1). CO stripping and in situ Fourier transform infrared spectroscopy (FTIR) experiments indicate that the alloying of Cu enhanced the adsorption of ethanol, accelerated the subsequent oxidation of intermediate species, and increased the resistance to CO poisoning of PtCu NCs/CB, as compared with commercial Pt/C. Therefore, alloying Pt with earth-abundant Cu under rational composition and surface control can optimize its surface and electronic structures and represents a promising strategy to promote the performance of electro-catalysts while reduce the use of precious metals.Keywords:Pt–Cu alloy;nanocubes;electrocatalysis;ethanol oxidation reaction;in situ FTIR
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）