MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-17
·
IF 5.3
Liang Jintao,Li Canru,Zhou Yu,Fan Lingling,Liu Lin,Liang Jianlu,Tan Xiaohong,Zhou Zhide,Li Guiyin
Golgi protein 73 (GP73) is highly expressed in hepatocellular carcinoma (HCC) and considered a sensitive serum biomarker for HCC diagnosis. Herein, a label-free electrochemical aptasensor for ultrasensitive detection GP73 based on nitrogen-doped reduced graphene oxide-ferrocene-platinum@palladium nanoparticles (NRGO-Fc-Pt@Pd NPs) was constructed. The NRGO-Fc-Pt@Pd NPs offered excellent conductivity, biocompatibility, and large surface area, serving as both an electrode modifier and a redox probe due to Fc's good electroactivity. GP73 aptamer (GP73 Apt ) was covalently bound with the NRGO-Fc-Pt@Pd NPs to form the signal probe. When GP73 specifically bound to GP73 Apt to form GP73-GP73 Apt complex and fell off from the electrode, it leads to an increased Fc(Fe(II)/Fe(III)) peak current in differential pulse voltammetry (DPV) due to the enhancing of the sensing interface's electroactivity. Under optimized conditions, the label-free electrochemical aptasensor demonstrated a linear relationship with GP73 concentration in the range 1.0 ng/mL-300 ng/mL, with R 2 of 0.99594 and a limit of detection (LOD) of 0.3524 ng/mL. In addition, the aptasensor showed good specificity, reproducibility, and stability. Furthermore, the developed aptasensor was applied to detect the GP73 in actual serum samples with good recoveries (97.85%-107.48%) and relative standard deviations (RSDs) (0.37%–7.85%) via the spiked recovery method. All the results may provide a new approach for HCC clinical detection. Graphical
研究领域:
生物医学工程
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肿瘤学
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分析化学
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纳米材料
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DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 3
Yao Tian,Mengsi Liang,Yongdong Wei,Yuxin Wang,Ning-Bo Li,Yaoyong Wang
Zearalenone (ZEN) is a carcinogenic mycotoxin commonly found in grains, cereals, and dairy products, posing cancer risks to humans and animals. Chronic high-dose ZEN exposure can cause liver disease, immunosuppression, kidney damage, and tumorigenesis. Sensitive and efficient ZEN detection is therefore essential for protecting human and animal health. To meet the need for sensitive, selective, and low-cost mycotoxin sensors in real food samples, we developed a novel ratiometric electrochemical aptamer sensor for ZEN detection. The sensor design utilizes β-cyclodextrin-functionalized graphene (CG) to effectively enhance electron transfer efficiency, combined with host–guest recognition between ferrocene (Fc) and β-cyclodextrin (β-CD), as well as Au-S covalent bonding. The 5′-SH and 3′-MB-modified dual-labeled ZEN aptamer (MB-Apt-SH) was immobilized via β-CD/Fc inclusion, with signal amplified by AuNPs binding to -SH. Without ZEN, the aptamer stays on the electrode, giving high IMB and low IFc. With ZEN, it binds the target, pulling it away. Adding Fc-COOH fills the β-CD cavities, increasing IFc and decreasing IMB, thus raising the IFc/IMB ratio proportionally to ZEN concentration. The sensor exhibits a wide linear range from 1 pg/mL to 1000 ng/mL, with a detection limit of 0.4344 pg/mL. Furthermore, the method was successfully applied to the determination of ZEN in wine samples, yielding recovery rates between 94.00% and 104.60%, demonstrating excellent accuracy and applicability. These results indicate that the MCH/AuNPs/SH-Apt-MB/CG/GCE platform is a highly sensitive and selective electrochemical sensor for ZEN detection.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Guiyin Li,HaiMei Li,Xinhao Li,Huapeng Huang,Huimin Bian,Jintao Liang,Zhide Zhou
Low-density lipoprotein (LDL) is a key biomarker involved in cardiovascular disease (CVD) risk assessment. Early-stage diagnosis of CVD complications by monitoring LDL levels could be a significant clinical tool and the first step toward adopting efficient therapy. Herein, a novel label-free electrochemical aptasensor for LDL detection was developed via the molybdenum disulfide-gold nanoparticle- ferrocene-carboxylic acid nanosheets (MoS 2 -Au-Fc NSs) enhanced signal amplification strategy. The MoS 2 -Au-Fc NSs were used not only as the biocompatible substrate for LDL aptamer (LDLapt) stabilization due to the increasing electrical conductivity and effective surface area, but also as a redox probe for monitoring the changes of the electrochemical signal because of good electroactive properties of Fc . The aptasensor fabrication steps were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). When LDL was captured onto the sensor via the specific affinity of the LDLapt, the formed LDLapt-LDL complexes shed from the electrode and enhanced the electron transfer rate on the electrode surface, resulting in an increase of the peak current. Thus, LDL can be easily detected by measuring the changes of peak current. Under ideal circumstances, the aptasensor was linearly correlated with logarithm LDL concentration from 0.001 to 100.0 μg/mL with R 2 of 0.9914, and a low detection limit of 0.42 ng/mL. Furthermore, the LDL detected by aptasensor in actual serum samples with good actual errors (0.73%–5.00%) and satisfactory relative standard deviations (RSDs) (0.04%–0.84%). This study provides a new analytical method for measuring the level of LDL which is specifically up-regulated in CVD patients at an early stage.
研究领域:
分析化学
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生物传感器
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纳米技术
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心血管疾病诊断
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DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaoyang Wang,Huajie Yu,Qiuhong Li,Yuhang Tian,Xiaolin Gao,Wanqi Zhang,Zhicong Sun,Yaoting Mou,Xia Sun,Yemin Guo,Falan Li
A novel dual-signal fluorescent sensor was developed for detecting organophosphorus pesticides (OPs). It relies on the catalytic activities of acetylcholinesterase (AChE) and choline oxidase (ChOx) to generate hydrogen peroxide (H 2 O 2 ) through the conversion of acetylcholine (ACh) to choline·H 2 O 2 then oxidizes ferrocene-modified tetraphenylethylene (TPE-Fc) to its oxidized state (TPE-Fc + ), resulting in enhanced cyan fluorescence due to aggregation. Simultaneously, ferrocene oxidation generates hydroxyl radicals (•OH), causing a decrease in orange fluorescence of glutathione-synthesized gold nanoclusters (GSH-AuNCs). The presence of OPs restricts AChE activity, reducing H 2 O 2 production. Increasing OPs concentration leads to decreased cyan fluorescence and increased orange fluorescence, enabling visual OPs detection. The sensor has a linear dynamic range of 10–2000 ng/mL with a detection limit of 2.05 ng/mL. Smartphone-based color identification and a WeChat mini program were utilized for rapid OPs analysis with successful outcomes.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2026
·
IF 4.9
Lan Jiang,Lina Zou,Jing Dong,Ping Zhang,Jian Zhou,Hao Jiang,Huanyu Ren,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Feiyan Yang,Lang Cheng,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Non-coding RNAs are critical tumor biomarkers, rendering innovations in their detection technologies highly significant. To overcome the limitations of conventional single-signal electrochemical biosensors (susceptibility to interference, insufficient stability), this study developed a novel ratiometric electrochemical DNA biosensor with dual-signal amplification. Its core novelty is the rational integration of two synergistic nanomaterials: Ferrocene-functionalized MgAl-layered double hydroxide (Fc@MgAl-LDH, as stable immobilization matrix and internal reference signal source) and AuNPs@Fc@MgAl-LDH coupled with MB-cDNA@AuNPs, which construct a cascaded signal amplification pathway. The sensing mechanism involves two-stage amplification: Primary by high-loading-capacity AuNPs@Fc@MgAl-LDH, secondary by MB-cDNA@AuNPs hybridizing with electrode-immobilized microRNA-21 (miRNA-21). The ratiometric signal ( I MB / I Fc ) enables built-in correction, boosting detection accuracy and reproducibility. After systematic optimization, the sensor has a wide linear range (1.0 fM–0.1 nM) and an ultra-low LOD of 0.757 fM, eliminating RNA extraction/enzymatic amplification to simplify operation. Serum validation shows high accuracy (95.3%–101.4% recovery), indicating strong clinical diagnostic application potential.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Juncong Miao,Kang Du,Xuan Li,Xiaoting Xu,Xue Dong,Jinglong Fang,Wei Cao,Qin Wei
Procalcitonin (PCT) as a disease marker is of great significance in the early diagnosis of septicemia and pyemia. Biosensor have good prospects for analysis and detection of disease markers, but developing highly sensitive detection methods for detecting PCT remains a daunting task. In this paper, we develop a ratiometric electrochemical immunosensor with Au NPs modified SiO 2 -Fc-COOH complex as matrix and UiO-66 loaded with Toluidine blue (TB) as a marker for quantitative detection of procalcitonin (PCT). The SiO 2 modified by APTES not only has a large specific surface area but also contains abundant amino groups, which can be connected to the electrochemical probe of ferrocenecarboxylic acid (Fc-COOH). UiO-66 has the advantages of large specific surface area, high porosity and adjustable structure, which can adsorb toluidine blue electrochemical probe through electrostatic attraction . Measurement and analysis of the prepared immunosensor by Differential Pulse Voltammetry (DPV) show that the oxidation peak currents of Fc-COOH and TB appear at potentials of 0.30 V and −0.30 V respectively. As the PCT concentration increases, the oxidation peak current of TB increases and the oxidation peak current of Fc-COOH decreases. The ratios ( ΔI = ΔI TB /ΔI Fc-COOH ) between the double signals could show a certain linear relationship with the concentration of the PCT within a certain range. Under optimal conditions, the linear range of detection obtained by the immunosensor was 1 pg/mL-100 ng/mL and the detection limit was 0.3 pg/mL. In this work, the developed ratiometric electrochemical immunosensor not only provides a simple, reliable and sensitive strategy for quantitative detection of PCT but also provides a useful method for clinical detection of other disease markers.
研究领域:
生物传感器
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医学检测
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电化学分析
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 17
·3344-3356
·
IF 7.571
Linxue Zhang,Zhuo Wan,Zuoying Yuan,Jun Yang,Yunfan Zhang,Qing Cai,Jianyong Huang,Yuming Zhao
Multicellular aggregates have been widely utilized for regenerative medicine; however, the heterogeneous structure and undesired bioactivity of cell-only aggregates hinder their clinical translation. In this study, we fabricated an innovative kind of microparticle-integrated cellular aggregate with multifunctional activities in angiogenesis and osteogenesis, by combining stem cells from human exfoliated deciduous teeth (SHEDs) and bioactive composite microparticles. The poly(lactide-co-glycolide) (PLGA)-based bioactive microparticles (PTV microparticles) were ∼15 μm in diameter, with dispersed β-tricalcium phosphate (β-TCP) nanoparticles and surface-modified vascular endothelialcadherin fusion protein (hVE-cad-Fc). After co-culturing with microparticles in U-bottomed culture plates, SHEDs could firmly attach to the microparticles with a homogeneous distribution. The PTV microparticle-integrated SHED aggregates (PTV/SHED aggregates) showed significant positive CD31 and ALP expression, as well as the significantly upregulated osteogenesis makers (Runx2, ALP, and OCN) and angiogenesis makers (Ang-1 and CD31), compared with PLGA, PLGA/β-TCP (PT) and PLGA/hVE-cad-Fc (PV) microparticle-integrated SHED aggregates. Finally, in mice, 3 mm calvarial defects filled with the PTV microparticle-integrated SHED aggregates achieved abundant vascularized neo-bone regeneration within 4 weeks. Overall, we believe that these multifunctional PTV/SHED aggregates could be used as modules for bottom-up regenerative medicine, and provide a promising method for vascularized bone regeneration.
研究领域:
再生医学
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组织工程
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生物材料
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干细胞生物学
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DOI
PubMed
高影响因子
Advanced Healthcare Materials
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2026
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IF 11
Zhong Cheng,Zhanyuan Qu,Jiaxin Sun,Zhihua Duan,Yong An,Yan Zhang,Jun Yang
Renal fibrosis lacks experimentally tractable human-relevant models that integrate defined stromal programming with spatially controlled profibrotic cues. Here, we developed a dual E-/VE-cadherin-Fc (EVE) interface to condition human mesenchymal stem cells (MSCs) under CTGF/TGF-β stimulation. Cells showed increased expression of Gli-1, NG2, PDGFRβ, and FAP and activated transcription of extracellular matrix remodeling. These changes were consistent with the acquisition of a Gli-1 + perivascular-like profibrotic stromal phenotype, and were accompanied by reorganization of cadherin-catenin complexes, actin cytoskeletal rearrangement, and increased YAP nuclear localization. Cell-sized PLGA/chitosan-heparin microparticles were subsequently functionalized with E-/VE-cadherin-Fc and loaded with CTGF/TGF-β. Co-assembly of these microparticles with the conditioned stromal cells (gMSCs) and renal epithelial cells (HK-2) generated 3D renal fibrotic microtissues that combined cadherin-mediated adhesive presentation with localized cytokine delivery. The resulting microtissues exhibited a broader distribution of α-SMA, Collagen I, and Fibronectin, together with reciprocal epithelial-stromal signaling and spatially organized fibrotic activation. As a proof of concept for pharmacological evaluation, the microtissues responded to pirfenidone (PFD) in a dose-dependent manner, with 100 µM PFD reducing α-SMA expression by ∼73% while preserving microtissue viability. Together, these findings establish a material-enabled, cadherin-guided strategy for constructing disease-relevant stromal organization and spatially controlled profibrotic signaling in human renal fibrosis models.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiajun Yu,Xiao Wang,Shijie Yang,Xiaofeng Xie,Jing Sun
Ligand functionalization could effectively modulate the local electron distribution of MOFs, thus improving their performance in both the capturing and photocatalytic oxidization of various VOCs. In this work, a series of Fc-CHO/NU-X (X = 1, 2, 3, 4) were obtained through the ligand functionalization of reo -NH 2 -UiO-66 (denoted as NU) with ferrocenecarboxaldehyde (Fc-CHO) as the modifier. By altering the local charge distribution and inducing Fe-OH and cyclopentadienyl rings as new adsorption sites, Fc-CHO altered the adsorption behavior of both aromatic and oxygenated VOCs and consequently their degradation pathways. The introduction of electron-donating Fc-CHO enhanced the ligand to metal charge transfer (LMCT), accelerated the electron-hole separation and therefore promoted the effective formation of oxidative radicals, facilitating the effective oxidation of acetaldehyde. Direct charge transfer through p-p interaction enabled the ring-opening and deep oxidation of toluene. The degradation efficiencies of toluene and acetaldehyde increased from 25 % and 28 % (NU) to 80 % (Fc-CHO/NU-3) and 65 % (Fc-CHO/NU-2), respectively. Positive effects of Fc-CHO functionalization were also observed during the removal of acetaldehyde-toluene mixtures and the synergistic effect between acetaldehyde and toluene was revealed. This work provides new ideas and novel materials for the purification of in-door air pollutants.
研究领域:
环境化学
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材料科学
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光催化
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纳米技术
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空气净化
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产品关联
DOI
高影响因子
JOURNAL OF DRUG TARGETING
·
2021
·
IF 5.121
De-Sheng Liang,Zu-Jun Wen,Jia-Hui Wang,Fang-Fang Zhu,Feng Guo,Jian-Liang Zhou,Jian-Jun Xu,Hai-Jun Zhong
Tumour-associated macrophages (TAMs) represent an attractive cell target for anticancer therapy. However, selective and efficient targeting of TAMs remains difficult. Here, we constructed a novel dually functionalised nanoparticle platform (s-Tpep-NPs) by surface co-modification of nanoparticles (NPs) with tuftsin (Tpep) and legumain protease-sheddable polyethylene glycol 5k (PEG5k) to achieve selective targeted delivery to TAMs. The fluorescence resonance energy transfer experiment and in vitro cellular uptake assay confirmed that s-Tpep-NPs can responsively shed PEG5k and transform into active Tpep-NPs upon the cleavage of legumain that is overexpressed on TAM surfaces, which then promotes TAM phagocytosis through Fc receptor-mediated pathways. Owing to the shielding effect by legumain-sheddable PEG5k, s-Tpep-NPs can effectively decrease the Tpep-induced non-specific accumulation in mononuclear phagocyte system (MPS) organs during systemic circulation. Moreover, s-Tpep-NPs can significantly enhance the tumoural accumulation and improve the specificity and efficiency of targeting to TAMs, as compared with both controls of Tpep-NPs and non-sheddable ns-Tpep-NPs. Overall, this study provides a robust nanoplatform with a novel avenue for improved selectivity of targeted delivery to TAMs.
研究领域:
药学
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纳米技术
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癌症研究
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生物医学工程
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Qinqin Chen,Xing Jin,Qifan Zheng,Min He,Liqiao Han,Jun Yan,Min Zhan,Pengwei Zhang,Peifeng Ke,Jianbing Wang,Xianzhang Huang,Qiaoxuan Zhang
High-affinity anti-double-stranded DNA (anti-dsDNA) antibodies serve as essential biomarkers for systemic lupus erythematosus (SLE), exhibiting particularly strong correlations with active disease manifestations and renal pathology. However, conventional detection methods require urea treatment to quantify these antibodies, necessitated by the heterogeneity of endogenous antibodies, which complicates assay procedures and compromises accuracy. In this study, a novel ratiometric electrochemical (EC) biosensor for the direct detection of high-affinity anti-dsDNA antibodies in serum without additional sample processing has been developed. This biosensor conducted by a novel T(6–4)T dimer dsDNA capture probe and integrated with a self-assembled 4-mercaptophenylboronic acid@gold nanoparticles@6-ferrocenyl-1-hexanethiol (4-MPBA@AuNPs@6-(Fc)HT) composite signal tag. Leveraging the highly specific of the T(6–4)T dimer dsDNA capture probe and multi-amplification capability of 4-MPBA@AuNPs@6-(Fc)HT, the platform exhibited non-specific interferences, higher sensitivity (limit of detection:1.60 IU/mL), and efficient preparation. Clinical serum analysis showed good correlation with indirect immunofluorescence (IIF) (r = 0.629, p < 0.001), superior diagnostic performance (AUC = 0.893 vs. IIF AUC = 0.900) in differentiating SLE patient from healthy control (CON). Notably, EC results correlated more strongly with renal impairment (r = 0.426, p < 0.01) than IIF (r = 0.321, p > 0.05), highlighting its superior clinical utility for monitoring disease. This strategy provides a robust and straightforward approach for serum high-affinity anti-dsDNA antibodies detection, offering significant potential for SLE diagnosis and progression monitoring.
研究领域:
生物医学工程
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免疫诊断学
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电化学传感器
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自身免疫性疾病
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纳米生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Haotian Wu,Li Tian,Haijuan Qin,Xiao Zhou,Xiying Chen,Weiran Li,Jing Zhang,Shuo Wang,Yaqing Liu
An injectable hydrogel dressing, Zr/Fc-MOF@CuO 2 @FH, was constructed by combing acid-triggered chemodynamic treatment (CDT) with low-temperature photothermal treatment (LT-PTT) to effectively eliminate bacteria without harming the surrounding normal tissues. The Zr/Fc-MOF acts as both photothermal reagent and nanozyme to generate reactive oxygen species (ROS). The CuO 2 nanolayer can be decomposed by the acidic microenvironment of the bacterial infection to release Cu 2+ and H 2 O 2 , which not only induces Fenton-like reaction but also enhances the catalytic capability of the Zr/Fc-MOF. The generated heat augments ROS production, resulting in highly efficient bacterial elimination at low temperature. Precisely, injectable hydrogel dressing can match irregular wound sites, which shortens the distance of heat dissipation and ROS diffusion to bacteria, thus improving sterilization efficacy and decreasing non-specific systemic toxicity. Both in vitro and in vivo experiments validated the predominant sterilization efficiency of drug-resistant methicillin-resistant Staphylococcus aureus (MRSA) and kanamycin-resistant Escherichia coli (KREC), presenting great potential for application in clinical therapy.
研究领域:
纳米医学
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生物材料
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生物医学工程
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传染病学
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材料科学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）