FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Mengke Zhang,Mengyin Li,Yan Zhao,Naifeng Xu,Lanlan Peng,Yuanfeng Wang,Xinlin Wei
In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe 3 O 4 /Au nanoparticles (Fe 3 O 4 /AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe 3 O 4 /AuNPs was developed. The results showed that the color intensity of test line positively correlated with the PV concentration in the standard or spiked sample. The limit of detection for qualitative (LOD) and quantitative detection (LOQ) were 2 ng/mL and 0.691 ng/mL, respectively. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80–120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale determination of PV in foodstuffs.
研究领域:
免疫学
·
食品科学
·
纳米技术
·
过敏学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Wenjing Yu,Jining Li,Guorong Huang,Zhe He,Huiyan Tian,Fengxin Xie,Weidong Jin,Qing Huang,Weiling Fu,Xiang Yang
Staphylococcus aureus ( S. aureus ) poses a serious threat to global public health, necessitating the establishment of rapid and simple tools for its accurate identification. Herein, we developed a terahertz (THz) metamaterial biosensor based on aptamer-functionalized Fe 3 O 4 @Au nanocomposites for quantitative S. aureus assays in different clinical samples. Fe 3 O 4 @Au@Cys@Apt has the dual advantages of magnetism and a high refractive index in the THz range and was used to rapidly separate and enrich target bacteria in a complex environmental solution. Furthermore, conjugation to the nanocomposites significantly increased the resonance frequency shift of the THz metamaterial after target loading. Our results showed that the shifts in the metamaterial resonance frequency were linearly related to S. aureus concentrations ranging from 1.0 × 10 3 to 1.0 × 10 7 CFU/mL, with a detection limit of 4.78 × 10 2 CFU/mL. The biosensor was further applied to S. aureus detection in spiked human urine and blood with satisfactory recoveries (82.4–109.6%). Our approach also demonstrated strong concordance with traditional plate counting (R 2 = 0.99306) while significantly lowering the analysis time from 24 h to <1 h. In conclusion, the proposed biosensor can not only perform culture-free and extraction-free detection of target bacteria but can also be easily extended to the determination of other pathogenic bacteria, rendering it suitable for various bacteria-related disease diagnoses.
研究领域:
医学诊断
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Guifen Jie,Junjun Ge,Xiaoshan Gao,Chunli Li
In this work, we designed a new strategy for ultrasensitive detection of CEA based on efficient electrochemiluminescence (ECL) quenching of Ru(bpy) 3 2+ -doped SiO 2 nanocomposite by ferrocene using target recycling amplification technique. A large number of [email protected] 2 ECL signal probe were firstly assembled on the novel magnetic core−shell Fe 3 O 4 @Au nanoparticles (NPs), then the ferrocene-labeled ECL quenching probe (Fc-probe) was linked to the magnetic NPs. Finally, numerous DNA1 sequences were produced by target CEA-triggered multiple recycling amplification and displaced the Fc-probe on the magnetic NPs, leading to significantly enhanced ECL signal for CEA detection. Because of the designed cascade signal amplification strategy, the newly developed method achieved a wide linear range of 10 fg/mL to 10 ng/mL with a low detection limit of 3.5 fg/mL. Furthermore, taking advantages of the magnetic Fe 3 O 4 @Au NPs for carring abundant signal probes, sensing target and ECL detection, the developed ECL strategy is convenient, rapid and displayed high sensitivity for CEA detection, which has great potential for analyzing the clinical samples in practical disease diagnosis applications.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 4
·799-805
·
IF 4.2
Shixin Cai,Xin Chen,Haohan Chen,Yuting Zhang,Xiaoli Wang,Nandi Zhou
The development of sensitive adenosine triphosphate (ATP) sensors is imperative due to the tight relationship between the physiological conditions and ATP levels in vivo. Herein, a fluorescent aptasensor for ATP is presented, which adopts a strategy that combines a split aptamer and a DNAzyme/walker with terminal deoxynucleotidyl transferase (TDT)-assisted formation of DNA-AgNCs to realize fluorescence detection of ATP. A multifunctional oligonucleotide sequence is rationally designed, which integrates a split aptamer, a DNAzyme and a DNA walker. Both multifunctional oligonucleotide and its substrate strand are connected to the surface of Fe3O4@Au nanoparticles via Au–S bonds. The existence of ATP can induce the formation of the complete aptamer, and then activate the DNAzyme to circularly cleave the substrate strand, leaving 2′,3′-cyclophosphate at the 3′end of the strand. This blocks the polymerization of dCTP to form poly(C) even in the presence of TDT and dCTP, due to the lack of free 3′-OH. In contrast, when ATP is absent, the DNAzyme/walker cannot work and then TDT catalyzes the formation of poly(C) at the free 3′-OH of the substrate strand, which is subsequently utilized as the template to prepare DNA-AgNCs. The fluorescence response derived from AgNCs thus reflects the ATP concentration. Under the optimum conditions, the aptasensor shows a linear response range from 5 nM to 10 000 nM, with a detection limit of 0.27 nM. The level of ATP in human serum can be effectively measured by the aptasensor with good recovery, indicating its application potential in medical samples.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Wang Ying,Sun Jingyi,Zhao Peng,Yi Hui,Yuan Hui,Yang Mingfeng,Sun Baoliang,Che Fengyuan
The blood–brain barrier (BBB) is of vital importance to the progression and prognosis of subarachnoid hemorrhage (SAH). The construction of a simple, sensitive, and accurate detection assay for measuring the biomarkers associated with BBB injury is still an urgent need owing to the complex pathogenesis of SAH and low expression levels of pathological molecules. Herein, we introduced surface-enhanced Raman scattering (SERS) label-embedded Fe3O4@Au core-shell nanoparticles as ideal SERS sensors for quantitative double detection of MMP-9 and occludin in SAH patients. Meanwhile, utilizing the SERS signals to dynamically estimate MMP-9 and occludin concentration in the rat SAH model is the first application in exploring the relationship of pathological MMP-9 and occludin molecular levels with neurobehavioral score. This method warrants reliable detection toward MMP-9 and occludin with a wide recognition range and a low detection limit in blood samples. Furthermore, the results monitored by the SERS assay exactly matched with those obtained through a traditional enzyme-linked immunosorbent assay (ELISA). The aforementioned results demonstrated this novel biosensor strategy has extensive application prospects in the quantitative measurement of multiple types of biomolecules in body fluid samples.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xinran Yang,Zonglin Li,Junhui Du,Xueli Zhang,Haibin Liu,Xuechao Zhang,Lijun Li,Chuanjin Cui,Hongshuo Chen
In this paper, a SERS/electrochemical dual-channel aptamer biosensor was established for ultrasensitive detection of Pb 2+ . Firstly, the capture probe Fe 3 O 4 @Au@PAMAM@Aptamer and the signal probe AuNs@4-MBA@Au@ssDNA were chemically synthesized step by step. Secondly, the carboxyl graphene oxide (GO) was electrodeposited onto the electrode surface to gain the GO/SPCE. Meanwhile, PAMAM@Au nanomaterials were successfully synthesized and thus prepared the Aptamer/PAMAM@Au/GO/SPCE. During the process, SEM, TEM, FTIR and XPS were applied to characterize the synthesized nanomaterials which confirmed the successful synthesis of the nanomaterials. Finaly, the performance of this biosensor was evaluated in a series of Pb 2+ aqueous solutions by surface enhanced Raman (SERS) and differential pulse voltammetry (DPV). The results showed that the lowest limit of detection (LOD) was 3.16 × 10 −5 nM, and the sample recovery was 99.50–103.67 %. Furthermore, the biosensor demonstrated good specificity, repeatability, and stability. Reliable results were also obtained in actual sample testing. Therefore, it can provide a viable method for trace detection of heavy metal ions.
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jun Li,Yao-Yao Fan,Jie Wen,Jing Zhang,Zhi-Qi Zhang
The HIV-Ι trans-activation responsive (TAR) RNA–trans-activator of transcription (Tat) protein complex is crucial for the efficient transcription of the integrated human immunodeficiency virus-I genome and is an established therapeutic target for AIDS diagnosis and treatment. Developing a sensitive strategy for the TAR RNA-binding ligand assay could provide antiviral leads with a radically new mechanism for the treatment of AIDS. Herein, a new TAR RNA-binding ligand assay platform was established using a signal amplification strategy that combines aggregation-induced emission (AIE) with a metal-enhanced fluorescence (MEF) concept. The tetraphenylethylene (TPE) derivative was labeled on the Tat peptide as a fluorescent molecule, while the TAR RNA was immobilized on the surface of the Fe3O4@Au@Ag@SiO2 nanoparticles (NPs) to specifically bind the TPE-Tat peptide. The TPE-Tat peptide was weakly emissive itself while emitting strongly in the NP–TAR–TPE-Tat complex by the AIE and MEF signal amplification effect. It was confirmed by known Tat peptide competitors that this system could be applied to the screening and detection of TAR RNA-binding ligands because they could replace the TPE-Tat peptide from the complex and make the system fluorescence decrease. When this system was adopted to test four candidate ligands, it was found that bisantrene had a favorable TAR RNA-binding ability. The proposed AIE–MEF strategy not only provides a sensitive and reliable method for the TAR RNA-binding ligand assay but also can avoid the influence of ligands on fluorescent detection in the conventional displacement assay.
研究领域:
病毒学
·
分子生物学
·
生物医学工程
·
化学生物学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-10
·
IF 6.232
Weng Chenyuan,Li Xiaoyun,Lu Qiaoyun,Yang Wei,Wang Jing,Yan Xiaoqiang,Li Bingzhi,Sakran Marwan,Hong Junli,Zhu Wanying,Zhou Xuemin
A highly sensitive and selective electrochemical biosensor for Pb 2+ with a dual-amplification strategy is proposed. The first amplification step was realized by the cycle of Pb 2+ and 8–17 DNAzyme (S2), and the hybridization chain reaction (HCR) triggered by S1 further amplified the electrochemical signal. Fe 3 O 4 @Au NPs, as a multifunctional magnetic carrier, is not only manifested in the construction of a magnetically controlled electrochemical response interface, but also has significant contribution in the purifying system, reducing interference, increasing the specific surface area, and the DNA loading. The magnetic nanocomposites were characterized by TEM as spheres with particle size of around 39 nm. When there was no Pb 2+ , long double-strand DNA (dsDNA) is formed on the surface of Fe 3 O 4 @Au NPs by the S1-triggered HCR; in the presence of Pb 2+ , S2 is activated and S1 on the surface of magnetic biocomposites (Fe 3 O 4 @Au NPs-S1) is continuously cleaved with the cycle of Pb 2+ and S2, leading to a significant decrease of methylene blue (MB) absorbed on dsDNA. Such reverse dual-signal amplification strategy effectively increased the current difference and improved the sensitivity of the proposed sensor. The electrochemical signal of MB was obtained by differential pulse voltammetry (DPV) with preconcentration, showing a linear response toward Pb 2+ ranging from 50 pM to 1 μM with a detection limit of 15 pM. The proposed method has feasible applications in detecting other heavy metal ions based on other metal-dependent DNAzyme.
Graphical Abstract
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Shiyi Zhao,Yingxia Zhou,Lai Wei,Lihua Chen
Based on the successful design and preparation of Fe 3 O 4 @ [email protected] glycol (PEG)@chondroitin sulfate (CS) nanoparticles , a novel electrochemical biosensor was elaborately developed for MO determination by the drop coating method. This biosensor demonstrates ultra-low fouling properties in complex biological systems, especially in whole serum. Only 2.40% of the differential pulse voltammetry (DPV) signal changes are shown in the resistance test of electrode interface to 100% goat serum. The constructed biosensor also presented a good selectivity (DNA samples for M1, M2 and M3give a response around 14.2–21.4%of that obtained by the target analyte), reproducibility (the relative standard deviation (RSD) is only 4.82%) and storage stability (93.2% of the initial response is maintained after 20 day). Moreover, this biosensor exhibits high analytical performance with a wide line arranges (10 −17 M ∼ 10 −12 M) in buffer, even in 100% goat serum. In both cases, a low limit of detection (LOD) of 3.3 aM(S/N = 3)is showed. To our knowledge, a few examples reported the application of electrochemical biosensors to the direct ultrahigh sensitive determination of MO, especially in whole serum.
研究领域:
电化学
·
生物传感器
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Lizhu Zeng,Chenjia Lin,Peiqing Liu,Duanping Sun,Jing Lu
Cardiac troponin I (cTnI), a principal biomarker of acute myocardial infarction, possesses superior cardiac specificity and can provide a long detection window for cardiac injury. Although the potential of cTnI as a cardiac biomarker is promising, sensitive and rapid detection of cTnI remains challenging. In this work, a novel colorimetric biosensor was developed for cTnI detection based on multifunctional hybrid nanoprobes coupled with triple-aptamer-modified tetrahedral DNA nanostructures (TAPT-TDNs). First, we evaluated the affinity of four TAPT-TDNs with three vertices modified by two kinds of aptamers in different proportions. The four TAPT-TDNs exhibited much better affinity than the single-stranded aptamer for cTnI, which was attributed to the fact that TAPT-TDNs could improve the capture efficiency of cTnI with excellent interface density and constant support. Second, the selected TAPT-TDNs with highest affinity were immobilized on Fe 3 O 4 -Au magnetic nanoparticles as capture probes (named Fe 3 O 4 -Au-TAPT-TDNs). TAPT-TDNs and HRP were both labeled on NH 2 -MIL-101(Fe) to form signaling probes (named MIL-101(Fe)-HRP-TAPT-TDNs). Then, with the help of capture probes and signaling probes, the target protein was recognized and captured to form capture probe-cTnI-signaling probe sandwich-like structures, causing a decrease in signaling probes in the supernatant with magnetic separation and eventually leading to a visual color change. This colorimetric aptasensor displays excellent analytical performance toward cTnI with a detection limit of 27 pg·mL −1 . The anisotropic aptamer-mediated colorimetric sensor may serve as an alternative to conventional immunoassays for quick and accurate detection of cTnI in clinical samples.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
分子生物学
·
心脏病学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 12
·1046
·
IF 4.4
Xiangyang Li,Hean Qian,Jin Tao,Mingshuo Cao,Meng Wang,Wenlei Zhai
Extensive use of pesticides in agricultural production has been causing serious health threats to humans and animals. Among them, phorate is a highly toxic organophosphorus insecticide that has been widely used in planting. Due to its harmful effects on human and animal health, it has been restricted for use in many countries. Analytical methods for the rapid and sensitive detection of phorate residues in agricultural products are urgently needed. In this study, a new method was developed by combining surface-enhanced Raman spectroscopy (SERS) and immunochromatography assay (ICA). Hybrid magnetic Fe3O4@Au@DTNB-Ab nanoprobes were prepared by modifying and growing Au nanoseeds on an Fe3O4core. SERS activity of the nanoprobe was optimized by adjusting the concentration of the Au precursor. A rapid and sensitive assay was established by replacing the traditional colloidal gold-based ICA with hybrid SERS nanoprobes for SERS-ICA. After optimizing parameters including coating antibody concentrations and the composition and pH of the buffer solution, the limit of detection (LOD) for phorate could reach 1 ng/mL, with a linear range of 5~100 ng/mL. This LOD is remarkably lower than the maximum residue limit in vegetables and fruits set by the Chinese government. The feasibility of this method was further examined by conducting a spiking test with celery as the real sample. The result demonstrated that this method could serve as a promising platform for rapid and sensitive detection of phorate in agricultural products.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
农业科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2017
·Vol. 164
·期 9
·B421
·
IF 3.259
Jing Lu,Lin Wu,Yufang Hu,Sui Wang,Zhiyong Guo
A Faraday cage-type electrochemiluminescence (ECL) biosensor for ultrasensitive detection of cancer-associated microRNA-21 was constructed based on Fe3O4@Au magnetic nanoparticles (Fe3O4@Au MNPs) and multi-functionalized graphene oxide. The capture unit (Fe3O4@Au MNPs-cDNA) was prepared by immobilizing capture DNA (cDNA) on Fe3O4@Au MNPs, while the multi-functionalized graphene oxide acted as the signal unit ((sDNA&luminol)@GO). In this biosensor, the capture unit, which was used to catch the microRNA-21, could be easily attracted onto the magnetic glassy carbon electrode surface. After the hybridization of microRNA-21 with the capture unit, the signal unit was further hybridized to form a Faraday cage-type structure, in which the signal unit was directly fixed onto the electrode surface, resembling a huge net and even becoming a part of the electrode surface. All luminol molecules labeled on the signal unit could take part in the electrode reaction, thus greatly enhancing the ECL signal. The ECL intensity was found to increase with the logarithm of microRNA-21 concentrations from 1 fM to 10 pM with a detection limit of 0.3 fM. In addition, this proposed Faraday cage-type ECL biosensor can be used for practical analysis of microRNA-21 in human serum, providing an ultrasensitive detection platform for microRNAs in clinical diagnosis.
研究领域:
生物传感器
·
纳米技术
·
癌症诊断
·
电化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）