JOURNAL OF MEDICAL VIROLOGY
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2022
·Vol. 95
·期 1
·e28299
·
IF 20.693
Lu Lv,Leiliang Zhang
Monkeypox virus (MPXV) is a member of Orthopoxvirus in the Poxviridae family, causing a Public Health Emergency of International Concern. The number of cases and geographic range has increased significantly in 2022. Identification of MPXV-specific therapeutic targets is urgent. G-quadruplex (GQ) secondary structures attract great attention as potential targets for antiviral strategy. Whether GQs are present in the MPXV genome remains inconclusive. In this study, we aim to characterize the GQs encoded by MPXV. Through a series of biophysical experiments, we characterized the formation potential of MPXV-encoded GQs and evaluated the binding and stabilization abilities of GQ ligands including BRACO-19, pyridostatin, and TMPyP4 to GQs encoded by MPXV. Moreover, GQ ligands suppressed the gene transcription of MPXV sequences containing GQ. BRACO-19 and TMPyP4 were able to inhibit vaccinia virus replication. We demonstrated the existence of MPXV GQ and reinforced the idea that GQs could be novel antiviral targets. Targeting these GQ sequences with GQ-binding molecules may represent a new approach for MPXV therapy.
研究领域:
病毒学
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分子生物学
·
药物开发
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 2
·493
·
IF 4.2
Ligang Ma,Chao Lin,Wenjun Jiang,Shun Yan,Huilin Jiang,Xiang Song,Xiaoqian Ai,Xiaoxiao Cao,Yihuan Ding
Selective supported catalysts have emerged as a promising approach to enhance carrier separation, particularly in the realm of photocatalytic hydrogen production. Herein, a pioneering exploration involves the loading of PdS and Pt catalyst onto g-C3N4nanosheets to construct g-C3N4@PdS@Pt nanocomposites. The photocatalytic activity of nanocomposites was evaluated under visible light and full spectrum irradiation. The results show that g-C3N4@PdS@Pt nanocomposites exhibit excellent properties. Under visible light irradiation, these nanocomposites exhibit a remarkable production rate of 1289 μmol·g−1·h−1, marking a staggering 60-fold increase compared to g-C3N4@Pt (20.9 μmol·g−1·h−1). Furthermore, when subjected to full spectrum irradiation, the hydrogen production efficiency of g-C3N4@PdS@Pt-3 nanocomposites reaches an impressive 11,438 μmol·g−1·h−1, representing an eightfold enhancement compared to g-C3N4@Pt (1452 μmol·g−1·h−1) under identical conditions. Detailed investigations into the microstructure and optical properties of g-C3N4@PdS catalysts were conducted, shedding light on the mechanisms governing photocatalytic hydrogen production. This study offers valuable insights into the potential of these nanocomposites and their pivotal role in advancing photocatalysis.
研究领域:
材料科学
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能源科学
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DOI
PubMed
高影响因子
ChemCatChem
·
2019
·Vol. 11
·期 24
·6372-6383
·
IF 4.495
Wenhua Ye,Jisong Hu,Xiaofeng Hu,Wenhua Zhang,Xinguo Ma,Huihu Wang
Graphical Photocatalysis : The traditional p–n junction charges transfer mode at the interface of CuInS 2 /g-C 3 N 4 can be switched to Z-scheme mode in CuInS 2 /Au/g-C 3 N 4 heterostructure through Au integration by weakening the built-in electric field and improving the interfacial bonding. Two dimensional (2D) heterojunction based on g-C 3 N 4 and CuInS 2 (CIS) nanosheets has been successfully constructed with the assistance of biomolecules L-cysteine. We demonstrated that a typical p–n junction charges transfer mode of CIS/PCN (protonated g-C 3 N 4 ) can be switched to Z-scheme transfer mode through the integration of Au by weakening the role of built-in electric field. The morphology of CIS/Au/PCN can be well adjusted through the formation of Au−S covalent bond and the electrostatic adsorption between Au and PCN during the hydrothermal process. Furthermore, the intimate interface contact inside CIS/Au/PCN heterojunction was indeed constructed, which may promote the charges separation and transfer process at the interface as evidenced by ESR spectra and time-resolved PL spectra. Under visible light illumination, the CIS/Au/PCN exhibited an enhanced CO (19.46 μmol/g) and CH 4 (1.18 μmol/g) yield in CO 2 reduction, which was about 5.04 times and 2.03 times higher than pure PCN. The composite heterojunction also showed excellent photocatalytic activity for tetracycline degradation with a 87 % degradation efficiency in 1 hour, which was 2.07 times higher than pure PCN. The experimental and theoretical calculation results demonstrated that an all-solid-state Z-scheme heterojunction was formed in the CIS/Au/PCN system, which was different from the typical p–n junction charges transfer mechanism in CIS/PCN.
研究领域:
材料科学
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环境科学
·
化学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Xin Li,Jingru Guan,Haopeng Jiang,Xianghai Song,Pengwei Huo,Huiqin Wang
Construction of multi-interface contact step-scheme (S-scheme) photocatalyst is a promising pathway to achieve high-electron transfer efficiency for photocatalytic CO 2 reduction. In this paper, g-C 3 N 4 nanosheets were selected as the main photocatalyst, rod-like CeO 2 (R-CeO 2 ) with unique Ce 4+ →Ce 3+ conversion property and rGO were loaded on the g-C 3 N 4 surface to construct 2D-1D-2D sandwich photocatalyst. The yields of CO and CH 4 were about 63.18 and 32.67 μmol/g after 4 h when the rGO/R-CeO 2 /g-C 3 N 4 was used as catalyst, which were about 4 and 6 times higher than that of pure CN, respectively. Cyclic experiments proved that the composite had excellent photocatalytic and material stability. Photoelectrochemical tests showed that the construction of S-scheme electron transfer model and the introduction of rGO can great enhance the electron transmission and separation of photogenerated electron-hole pairs. CO 2 adsorption test identified that the loading of R-CeO 2 and rGO obviously enhanced the CO 2 adsorption ability of pure g-C 3 N 4 . Density functional theory (DFT) calculations used to analyze the electron transfer path and the formation of the build-in electric field at the semiconductor interface. In-situ FTIR and 13 CO 2 element-tracer detection carried out to research the process of CO 2 photoreduction. A possible multi-interface contact S-scheme electron transfer mechanism for enhanced CO 2 photoreduction activity has been discussed.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
Catalysts
·
2024
·Vol. 14
·期 11
·756
·
IF 3.8
Xuekai Shan,Guolin Zhang,Ying Zhang,Shuobo Zhang,Fang Guo,Qi Xu
Selective CO2hydrogenation faces significant technical challenges, although many efforts have been made in this regard. Herein, a Ni-doped In2O3catalyst supported by g-C3N4was prepared using the co-precipitation method, and its composition, morphology, specific surface area, and band gap were characterized using TEM, XPS, BET, XRD, CO2-TPD, H2-TPR, UV-Vis, etc. The catalytic hydrogenation reduction of CO2to produce methanol was tested. Under low-photothermal conditions (1.0 MPa), the hydrogenation of carbon dioxide to methanol is stable, effective, and highly selective, with a spatiotemporal yield of 86.0 gMeOHh−1kgcat−1, which is 30.9% higher than that of Ni-In2O3without g-C3N4loading under the same conditions.
研究领域:
催化化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
SENSORS
·
2013
·Vol. 13
·期 1
·1064-1075
·
IF 1.953
Zhenzhu Zheng,Jing Han,Wensheng Pang,Juan Hu
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication ofPseudostellaria heterophylla(PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2−to blue-green ABTS•−by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9to 3.0 × 10−7mol·L−1(R2= 0.9906), with a low detection limit of 3.1 × 10−10mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.Keywords:G-quadruplex DNAzyme;molecular beacon;colorimetric;Pseudostellaria heterophylla
研究领域:
生物化学
·
分子生物学
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分析化学
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中药学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Hongbo Li,Mingbin Liu,Weihua Zhao,Jiamei Pu,Jianguo Xu,Suqin Wang,Ruqin Yu
Nucleic acids-sensing methods provide the potential to enable and hasten early disease detection and to rationalize treatments, especially for cancers. In this work, a colorimetric label-free sensing system based on target-triggered split-to-intact remodeling of dual-G-rich duplex probe (Dual-GDP) has been developed for amplified signaling of Pax-5 gene. In this sensing system, the Dual-GDP is splited into one hairpin-structured G-rich probe (HGP) and one duplex-shaped G-rich probe (DGP), which can be re-built to an intact form through target gene triggered accurate base-pairing. Significantly, this unique split-to-intact remodeling behavior allows the occurrence of a well-designed active isothermal amplification that can amplify target response with seven stages to multi-channel accumulate numbers of G-quadruplex transducers. When specifically combined with hemin, the formed G-quadruplex-hemin complexes, also known as DNAzymes, can strongly catalyze the oxidization of TMB to a colored TMB + and robustly reflect the target gene even in complex biological environments. We demonstrate that this method offers simplified operations and improved assay abilities for Pax5 gene analysis. We envision these findings might provide new insights to exploit versatile oligonucleotide probes and nucleic acid-based amplification strategies for diverse bioanalytical applications.
研究领域:
分子生物学
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分析化学
·
癌症诊断
·
核酸传感
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 11
·1-12
·
IF 2.8
Gao Shengqi,He Qin,Yang Qingshan,Wan Songhan,He Xianru
The NBR/G@Fe 3 O 4 composites with good electromagnetic shielding properties are prepared by solution blending, previously, the “G@Fe 3 O 4 composite particles” were obtained by a simple co-precipitation method. The structures of G@Fe 3 O 4 are characterized by Fourier infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectroscopy (Raman), Scanning electron microscopy (SEM), and the graphene content in the composite particles was analyzed by thermogravimetric analysis (TGA). The electromagnetic shielding performance of NBR composites is tested by microwave vector network analyzer. The results show that the average electromagnetic shielding effectiveness of NBR/G@Fe 3 O 4 composites in X-band (8.2–12.4 GHz) can reach 80.4dB, and when the thickness is only 2.1 mm and the filler content is 15 phr can reach 28.2dB, which shield 96.2% of the energy of incident electromagnetic waves. Due to the introduction of magnetic particle Fe 3 O 4 , the NBR/G@Fe 3 O 4 composites have better shielding performance than NBR/G composites at the same graphene content. The electromagnetic shielding effectiveness of NBR/G@Fe 3 O 4 composites with 9.3 phr of graphene is basically the same as that of NBR/G composites with 12.4 phr of graphene.
研究领域:
材料科学
·
电磁学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Songyu Yang,Kailin Wang,Zhen Wu,Yan Wu
Quantum dots/two-dimensional (0D/2D) semiconductor photocatalysts demonstrate wide solar light absorption region and high charge transfer efficiency. However, the relation between the interfacial electric field and the charge transfer during the photocatalytic hydrogen production process is still unclear. Here, we construct NiCo 2 O 4 quantum dots (QDs) and NiCo 2 O 4 nanoparticles (NPs) anchored with 2D g-C 3 N 4 (CN) to form NiCo 2 O 4 -QDs/CN and NiCo 2 O 4 -NPs/CN heterojunctions. The hydrogen production rate of CN loaded with NiCo 2 O 4 QDs is about 3 times higher than that of CN loaded with NiCo 2 O 4 NPs. The electric field intensity at the NiCo 2 O 4 -QDs/CN interface is calculated to be about 15,600 V cm −2 , about 9 times higher than that of NiCo 2 O 4 -NPs/CN, which could effectively drive the electrons of CN to flow toward NiCo 2 O 4 QDs, promoting photocarriers separation and hence greatly improving the photocatalytic performance. This work provides a method to understand the relationship between interfacial electric field strength and photogenerated charges of heterostructure photocatalysts.
研究领域:
材料科学
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能源科学
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产品关联
DOI
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2016
·Vol. 70
·期 3
·271-279
·
IF 1.427
Pengfei Xing,Rongxiang Zhao,Xiuping Li,Xiaohan Gao
The ultra-deep desulfurization of fuel oil has become inevitable for environmental protection. Here, CoWO4/g-C3N4 was used as a catalyst, H2O2 as an oxidant, and 1-ethyl-3-methylimidazolium ethylsulfate ([EMIM][EtSO4], IL) as an extractant for the oxidative desulfurization of model oil. Scanning electron microscopy, FT-IR spectroscopy, N2 adsorption isotherms, and X-ray diffraction were used to confirm the morphology, structure, and properties of the catalysts. The influence of calcination temperature, loading dose of cobalt, amount of H2O2, reaction temperature, and other parameters were investigated. The removal rate of sulfide in model oil could reach 92.9 % at 80°C in 180 min under the optimal operation conditions (V(oil) = 5 mL, T = 80°C, m(catalyst) = 0.03 g, V(H2O2) = 0.4 mL, t = 180 min, V(IL) = 1.0 mL). In addition, the catalyst was reused five times with no significant reduction in the catalytic activity.
研究领域:
环境科学
·
催化化学
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Mengyang Xu,Xiaoxue Zhao,Haopeng Jiang,Songtao Chen,Pengwei Huo
Converting carbon dioxide into valuable chemicals is seen as a promising strategy for slowing global warming while enriching energy supplies. Photocatalysts derived from Metal-Organic Frameworks (MOFs) can not only overcome the problem of poor stability of MOFs, but also inherit the advantages of MOFs to some extent. Herein, carbon doped In 2 O 3 (C-In 2 O 3 ) hollow tubular is synthesized by calcination of MIL-68(In) as sacrificial template. Then C-In 2 O 3 and g-C 3 N 4 are hydrothermal to obtain C-In 2 O 3 /g-C 3 N 4 heterojunction . The unique hollow tubular structure, carbon doping and type-II heterojunction of C-In 2 O 3 /g-C 3 N 4 expand the light absorption capacity and promote the separation of photogenerated electron-hole pairs, thus significantly improve the activity of photocatalytic reduction of CO 2 . Among them, C-In 2 O 3 /g-C 3 N 4 -5 shows the highest CO 2 reduction activity towards CO (153.42 μmol/g/h) and CH 4 (110.31 μmol/g/h).
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Zhenyu Wang,Yu Huang,Meijuan Chen,Xianjin Shi,Yufei Zhang,Junji Cao,Wingkei Ho,Shun Cheng Lee
The development of catalysts that effectively activate target pollutants and promote their complete conversion is an admirable objective in the environmental photocatalysis field. In this work, graphitic carbon nitride (g-C3N4) microtubes with tunable N-vacancy concentrations were controllably fabricated using an in situ soft-chemical method. The morphological evolution of g-C3N4, from the bulk to the porous tubular architecture, is discussed in detail with the aid of time-resolved hydrothermal experiments. We found that the NO removal ratio and apparent reaction rate constant of the g-C3N4 microtubes were 1.8 and 2.6 times higher than those of pristine g-C3N4, respectively. By combining detailed experimental characterization and density functional theory calculations, the effects of N-vacancies in the g-C3N4 microtubes on O2 and NO adsorption activation, electron capture, and electronic structure were systematically investigated. These results demonstrate that surface N-vacancies act as specific sites for the adsorption activation of reactants and photoinduced electron capture, while enhancing the light-absorbing capability of g-C3N4. Moreover, the porous wall structures of the as-prepared g-C3N4 microtubes facilitate the diffusion of reactants, and their tubular architectures favor the oriented transfer of charge carriers. The intermediates formed during photocatalytic NO removal processes were identified by in situ diffuse reflectance infrared Fourier transform spectroscopy, and different reaction pathways over pristine and N-deficient g-C3N4 are proposed. This study provides a feasible strategy for air pollution control over g-C3N4 by introducing N-vacancy and porous tubular architecture simultaneously.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）