INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Ruihong Wu,Ahmed Saud Abdulhameed,Ali H. Jawad,Salis Awal Musa,Yannis De Luna,Zeid A. ALOthman,Sameer Algburi
The present work develops an effective bioadsorbent of cross-linked chitosan-genipin/SiO 2 adsorbent (CHI-GNP/SiO 2 ). The developed CHI-GNP/SiO 2 was employed for the removal of organic dye (reactive orange 16, RO16) from simulated wastewater. The optimization of the fundamental adsorption variables (CHI-GNP/SiO 2 dose, time, and pH) via the Box-Behnken design (BBD) was attained for achieving maximal adsorption capacity and high removal efficiency. The good agreement between the Freundlich isotherms and empirical data of RO16 adsorption by CHI-GNP/SiO 2 indicates that the adsorption process follows a multilayer adsorption mechanism. The reasonable agreement between the pseudo-second-order model and the kinetic data of RO16 adsorption by CHI-GNP/SiO 2 was obtained. The maximum RO16 adsorption capacity ( q max ) of CHI-GNP/SiO 2 was identified to be 57.1 mg/g. The adsorption capacity of CHI-GNP/SiO 2 is attributed to its unique surface properties, including its highly porous structure and the presence of functional groups such as amino and hydroxyl groups. According to the results of this investigation, CHI-GNP/SiO 2 has the potential to be an adsorbent for the removal of acidic dyes from wastewater.
研究领域:
环境科学
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化学工程
·
材料科学
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DOI
PubMed
高影响因子
Tissue Engineering and Regenerative Medicine
·
2018
·Vol. 16
·期 1
·39-50
·
IF 1.216
Zhong Yi,Jiang Ai,Sun Fei,Xiao Yuanfan,Gu Ying,Wu Lei,Zhang Yujie,Shi Hongcan
Background: Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits. Methods: The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin–eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation. Results: The biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them ( p < 0.05). The histological staining results revealed that most of the mucosal epithelial cells were removed and that the decellularized trachea had lower immunogenicity than the control group. The analysis of SEM revealed that the decellularized trachea retained the micro- and ultra-structural architectures of the trachea and that the matrices cross-linked with genipin were denser. The biocompatibility evaluation and in vivo implantation experiments showed that the decellularized trachea treated with the DEM had better biocompatibility than that treated with the TPM and that immunogenicity in the cross-linked tissues was lower than that in the uncross-linked tissues ( p < 0.05). Conclusions: Compared with the trachea treated with the TPM, the rabbit trachea processed by the DEM had better biocompatibility and lower immunogenicity, and its structural and mechanical characteristics were effectively improved after the genipin treatment, which is suitable for engineering replacement tracheal tissue.
研究领域:
组织工程
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生物医学工程
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生物力学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Yibin Yu,Shuo Xu,Shihui Yu,Jinyu Li,Guoxin Tan,Sanming Li,Weisan Pan
We developed a hybrid nanostructured lipid carrier/dual pH- and thermo-sensitive hydrogel (NLC-Gel) for ocular delivery of quercetin (QN). The hydrogel consisted of carboxymethyl chitosan and poloxamer 407 (P407) was cross-linked using a naturally occurring cross-linker genipin (GP), while the hydrogel cross-linked using glutaraldehyde was used as a control. NLC loaded with quercetin/hydrogel cross-linked by a genipin hybrid drug delivery system (QN-NLC-Gel-GP) exhibited better cytocompatibility and lower ocular irritation than its glutaraldehyde counterpart. The levels of cellular uptake in NLC and NLC-Gel groups were improved. The results of fluorescence imaging and ex vivo transcorneal study indicated that NLC could facilitate transcorneal penetration of lipophilic molecules and incorporation into the hydrogel could increase the precorneal residence time of NLC. The area under the curve of quercetin in the NLC-Gel group was 4.4-fold that in the Eye drops group because of a longer precorneal retention time. In summary, the developed drug delivery system has enormous potential for ophthalmic applications.
研究领域:
药学
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材料科学
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眼科学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2018
·Vol. 106
·期 5
·1258-1268
·
IF 3.231
Xiaopeng Zhou,Yiqing Tao,Erman Chen,Jingkai Wang,Weijing Fang,Tengfei Zhao,Chengzhen Liang,Fangcai Li,Qixin Chen
Adipose-derived stem cells (ADSCs)-based tissue engineering was a promising method to treat intervertebral disc degeneration. Type II collagen is a native component in the nucleus pulposus (NP), and has the ability to promote ADSCs to differentiate into NP-like cells. In this article, we aimed to establish a genipin-cross-linked three-dimensional (3D) type II collagen scaffold, and determine the biological effects of the scaffold on ADSCs differentiating into a NP-like phenotype. Different concentrations of genipin were used to cross-link the 3D type II collagen scaffold. Microstructure, surface topography, mechanical strength, porosity, swelling property, and biological stability of the scaffolds were detected to evaluate the scaffold properties. Cell proliferation, gene and protein expression were measured to access the biological effects of the scaffolds on ADSCs, and the related molecular mechanism was investigated. Cross-linking by genipin increased the stability of the type II collagen scaffolds, but deformed the configuration of scaffolds and changed the intrinsic properties of type II collagen. scaffold cross-linked with 0.1% genipin improved the biostability on the basis of maintaining the configuration of scaffold. In addition, the 0.1% genipin-cross-linked scaffold promoted ADSCs proliferation and differentiation into NP-like cells, along with the increasing gene and protein expressions of Sonic Hedgehog (Shh). All these results suggested that 0.1% genipin was the optimal concentration to establish a bio-stable 3D type II collagen scaffold, which inducing ADSC proliferation and differentiation toward a NP-like phenotype through the activation of Shh signaling pathway. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1258–1268, 2018.
研究领域:
组织工程
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干细胞生物学
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骨科
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生物材料
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Qiang Liu,Na Xia,Wenjing Wan,Youcheng Gao,Siming Zhu
Novel eco-friendly and green dimethyldiallylammonium chloride (DMDAAC) grafted chitosan/genipin/cellulose hydrogel beads (CCBG-g-PDMDAAC), were fabricated as selective adsorbents for anionic dyes. The physical and chemical structural changes of the prepared hydrogels were evaluated by FTIR, XRD, SEM and TG-DSC analysis. Results showed CCBG-g-PDMDAAC efficiently and selectively adsorb anionic dyes (Reactive Red 195-RR195 and Methyl orange-MO) from mixture of dye solutions. Endowed chitosan-based hydrogels the advantage of acid insolubility and good adsorption. RR195 and MO adsorption process were described better with pseudo-second-order kinetic model and Langmuir isotherm model with a maximum adsorption capacity of 1333.52 and 190.48 mg/g, respectively, indicating that monolayer chemisorption controlled the sorption process. Moreover, the hydrogels exhibited nice reusability and against S. aureus and E. coli . The hydrogels are promising for the potential application in wastewater treatment and antibacterial simultaneously.
研究领域:
环境科学
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材料科学
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生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
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2016
·Vol. 23
·期 8
·156
·
IF 1.969
Zhang Wu,Ren Guangli,Xu Hao,Zhang Jinglin,Liu Huidi,Mu Shansong,Cai Xiang,Wu Ting
To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, the chitosan hydrogel had been prepared using genipin as the cross-linker and the tetracycline was in situ encapsulated. The formation process and strength of hydrogel were studied by rheological analysis, and the microtopography was observed by scanning electron microscope. It was found that the amount of genipin could modulate the physical property of the hydrogel. For in vitro release and antibacterial assays, the hydrogel could controllably release tetracycline and keep its bioactivity for a long time. The tetracycline-loaded chitosan hydrogel showed good antibacterial effect even under alkaline environment, which displayed better stability compared with free tetracycline. Moreover, the tetracycline-loaded chitosan hydrogel exhibited lower cytotoxicity than did tetracycline alone, suggesting that this tetracycline-loaded chitosan hydrogel could be a more useful dosage form than separate doses of tetracycline. The novel aspects of this study include the cytotoxicity study and the in vitro and in vivo assays, which might be useful for other researchers in this field.
To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, genipin cross-linked chitosan hydrogel was used as the carrier of tetracycline. The hydrogel could controllably release tetracycline with bioactivity. The hydrogel showed good antibacterial effect even under alkaline environment. The hydrogel exhibited lower cytotoxicity than did tetracycline alone.
研究领域:
药剂学
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生物材料
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药物递送
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组织工程
·
抗菌治疗
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Fei Teng,Xixi Wu,Yue Liu,Lijia Li,Mengjie Geng
Emulsion gel is widely used in the delivery of functional components because of its network structure and mechanical properties. In this study, to improve the structural and functional properties of emulsion gels and achieve efficient encapsulation and release of functional components, transglutaminase (TGase) and genipin were employed to crosslink pH-shifting/ultrasonication-modified soybean lipophilic protein (SLP), enabling the simultaneous encapsulation of vitamin E (VE, oil phase) and quercetin (QU, aqueous phase), thereby developing a co-encapsulated emulsion gel system. The impacts of combined modification (pH-ultrasonication) and enzymatic/chemical crosslinking on gel properties were systematically investigated through gel strength analysis, rheological characterization, intermolecular interaction profiling, microstructural observation, and in vitro digestion evaluation. Results demonstrated that pH-shifting combined with ultrasonication exposed more hydrophobic groups in SLP, thereby enhancing intermolecular hydrophobic interactions. Microstructural analysis revealed that TGase and genipin synergistically strengthened network crosslinking density, forming a more compact gel network structure under modified conditions. Furthermore, TGase/genipin crosslinking significantly improved structural characteristics and rheological performance (e.g., water-holding capacity increased by 14.97 %, hardness and viscosity improved), while concurrently enhancing encapsulation efficiencies of both VE and QU. In vitro digestion test confirmed that the maximum release rate reached 97.43 ± 1.50 % (VE) and 90.39 ± 2.43 % (QU) ( p < 0.05). These findings elucidated the potential for constructing emulsion gels via combined modification and crosslinking strategies, providing theoretical guidance for co-encapsulation systems in functional factors delivery applications.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Xiaoyu Du,Huaiyue Sun,Ye Zi,Cuiping Shi,Xichang Wang,Wei Cai,Jian Zhong
Herein, the protein-free algal oil@gum Arabic (GA)-chitosan (CS) powders were developed via the complex coacervation of GA with CS, and the effect of crosslinking agents (genipin, GNP; polymer tripolyphosphate, TPP) on the preparation and properties of the powders. The algal oil powders could be prepared at the coacervation pH of 5.5–4.0, the CS:GA mass ratio of 1:6, and the GA concentrations of 20–140 g/L. Both GNP and TPP crosslinking did not change the structures of the complex coacervates. The sizes of complex coacervates showed a crosslinking agent-dependent order: GNP > Control (CTL) > TPP. Moreover, the production yields, encapsulation yields, loading capacities, and encapsulation efficiencies of the algal oil powders were consistent with this order (GNP > CTL > TPP). However, the highest peroxide values at 63 °C (TPP, 784.6 mmol/kg oil > CTL, 647.8 mmol/kg oil > GNP, 572.0 mmol/kg oil) and free fatty acid release percentages (TPP, 72.5 % at 2 h > CTL, 49.0 % at 2 h > GNP, 43.0 % at 2 h) in the in vitro gastrointestinal model were reversely consistent with this order. This work would be beneficial for developing and applying oil powders in functional foods, especially in medical foods.
研究领域:
食品科学与技术
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微胶囊化
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功能食品
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胶体与界面化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Ying Pan,Longxiang Liu,Yanping Zhang,Lei Song,Yuan Hu,Shudong Jiang,Hongting Zhao
A crosslinked and flame retardant coating was fabricated by layer-by-layer self-assembly on cotton fabric. The coated fabric was treated using positively charged polyethylenimine (PEI) and negatively charged hypophosphorous acid-modified chitosan (HACH) with subsequent crosslinking with genipin. The thermal stability and flammability of the treated cotton fabric were investigated by thermogravimetric analysis (TGA), the horizontal flame test and microscale combustion calorimetry. TGA results showed that, in an air atmosphere, there was more char residue for the treated cotton fabrics than for the untreated cotton fabric. The cottons treated with a 10-bilayer coating (C-(PEI/HACH)10 and C-(PEI/HACH)10-crosslink) could extinguish the fire during the horizontal burning test. The peak heat release rate and total heat release rate of C-(PEI/HACH)10 were reduced by 73% and 80%, respectively, compared with those of the untreated one. At the same time, the genipin crosslinked PEI/HACH coating exhibited good wash durability and mechanical properites for the treated fabrics.
研究领域:
材料科学
·
纺织工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Min Wu,Yuyang Zhou,Ren-Cheng Tang
Integrating bio-extracts with natural fabric towards fully bio-based textiles is broadly advocated that benefits customers’ health and the environment. However, promoting the durability aside from using synthetic fixer or metal mordant, and exploring new source for blue bio-colorant are two challenging issues at this stage. To address this issue, a blue bio-colorant phycocyanin (PC) and a bio-crosslinker genipin (GNP) are combined to silk for durable blue appearance and UV protective/antioxidant functions. In the presence of GNP, the PC quantity on silk dramatically increases from 23.5 to 74.4 mg/g which enhances the colour depth and functionalities of silk. Moreover, the PC/GNP treated silk achieves high ratings in the washing and rubbing tests. Those two results demonstrate the dual roles of GNP for promoting the affinity/durability of PC to/on silk. The condition, i.e., 10 % owf of PC, 1 g/L GNP, 70 °C and 3 h is recommended considering the colour strength and fastness of silk, thermal stability of PC, reactivity of GNP and effective crosslinking between PC and silk. PC (10 % owf) imparts silk with high UPF value up to 39 and scavenges over 70 % of free radicals within 60 min. Almost no strength loss occurs on silk thanks to this milder processing condition. The designed PC, GNP and silk crosslinking system expands the applicable area of silk which represents the next-generation fully bio-based silk product.
研究领域:
纺织科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Ruifeng Luo,Meisi Lin,Chen Zhang,Jinfeng Shi,Siyuan Zhang,Qiyan Chen,Yichen Hu,Minyue Zhang,Jinming Zhang,Fei Gao
Curcumin (CUR) is a promising edible phytochemical compound with ideal ulcerative colitis (UC) treatment activity; however, it is characteristically instable in the digestive tract and has a short retention time in colon. Therefore, we designed and fabricated an oral food-grade nanocarrier composed of tannic acid (TA)-coated, Genipin (Gnp)-crosslinked human serum albumin (HSA) to encapsulate CUR (TA/CUR-NPs). The resulting CUR nanoparticles (NPs) were about 220 nm and −28.8 mV. With the assistance of TA layer and Gnp-crosslinking, the entire nano-scaled system effectively delayed CUR release in simulated gastric fluid, prolonged its colon adhesion and increased its uptake in Caco-2 cells. As expected, TA/CUR-NPs oral administration significantly alleviated colitis symptoms in DSS-treated mice when compared with controls by inhibiting the TLR4-linked NF-κB signaling pathway. Collectively, this study indicates that we have developed a convenient, eco-friendly, nano-scaled vehicle for oral delivery of CUR with anti-UC benefit.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
胃肠病学
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产品关联
DOI
PubMed
高影响因子
Materials
·
2018
·Vol. 11
·期 11
·2203
·
IF 2.467
Shufang Wu,Xunjun Chen,Minghao Yi,Jianfang Ge,Guoqiang Yin,Xinming Li
The high moisture sensitivity of feather keratin/polyvinyl alcohol/tris(hydroxymethyl)aminomethane (FK/PVA/Tris) blend films hinders their application in the packaging field. Thus, in order to improve the water resistance and mechanical properties of such blend films, we attempted cross-linking the blend film with cross-linking agents such as transglutaminase (TG), CaCl2, and genipin. Obvious differences in the morphology of the blended films were observed by scanning electron microscopy before and after cross-linking, indicating that cross-linking can inhibit the phase separation of the blend film. Conformational changes in the blend films after cross-linking were detected by Fourier transform infrared spectroscopy. Importantly, from examination of the total soluble mass, contact angle measurements, and water vapor permeability tests, it was apparent that cross-linking greatly improved the water resistance of the blend films, in addition to enhancing the mechanical properties (i.e., tensile strength and elongation at break). However, cross-linking was also found to reduce the oxygen barrier properties of the blend films. Therefore, cross-linking appears to be an effective method for promoting the application of FK/PVA/Tris blend films in the packaging field.Keywords:blend film;feather keratin;cross-linking;water resistance;mechanical properties
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）