REACTIVE & FUNCTIONAL POLYMERS
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2019
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IF 3.074
Jie-Ping Fan,Yu-Tong Cheng,Xue-Hong Zhang,Zhou-Peng Xiao,Dan-Dan Liao,Hui-Ping Chen,Kuan Huang,Hai-Long Peng
In this work, a novel mixed non-covalent and semi-covalent molecularly imprinted membrane (MIM) with hierarchical pores was prepared by cryopolymerization at −18 °C and using PEG2000 and dimethyl sulfoxide ice crystals as the pore templates. The free-standing MIM was characterized by scanning electron microscope and nitrogen adsorption-desorption isotherms, and the results confirmed the formation of the hierarchical pore structure . In the static adsorption, membrane permeation and dead-end filtration experiments, the results indicated that the mixed non-covalent and semi-covalent molecular imprinting was a good method to improve the performance of MIM which had a good adsorption selectivity toward genistein due to the presence of the imprinted sites in MIM and possessed strong imprinting efficiency toward genistein even in some similar compound mixture or extract of Radix Puerariae Lobatae.
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高影响因子
Innovative Food Science & Emerging Technologies
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2023
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IF 6.6
Xiao-Jia He,Jiao Jiao,Qing-Yan Gai,Jin-Xian Fu,Yu-Jie Fu,Zi-Yi Zhang,Jie Gao
A novel endophytic fungus Penicillium rubens isolated from pigeon pea producing cellulase (0.73 ± 0.14 U/mL) and β-glucosidase (4.85 ± 0.42 U/mL) was used to ferment pigeon pea roots (PPR) for obtaining bioactive genistein . Semi-solid-state fermentation (SSSF) with water was found to be the appropriate strategy for increasing genistein level in PPR. The genistein yield (0.37 ± 0.02 mg/g) in PPR fermented by P. rubens under the optimal conditions was 3.08-fold higher than that in the control (0.12 ± 0.02 mg/g). Scanning electron microscope results indicated that P. rubens utilized the nutrients in PPR for growth and could significantly destroy cell walls, thus contributing to the release of more genistein. The bioactivities of extracts from PPR fermented by P. rubens were higher than control. The present study provided a cost-effective SSSF approach to achieve higher yield of genistein from agrifood by-products PPR using the novel P. rubens . Industrial relevance A novel endophytic fungus P. rubens , capable of cell wall hydrolysis and genistin deglycosylation , was used to ferment PPR using SSSF strategy to obtain higher yield of bioactive genistein. The present study developed a method associated with advantages of simple operation, low cost, low energy consumption and environmental friendliness for the effective utilization of agrifood by-products PPR in food , nutraceutical, and other industrial fields. Also, P. rubens exhibited promising potential in fermentation fields for improving yields of other bioactive aglycones from food and medicinal resources.
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高影响因子
Food Bioscience
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2024
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IF 4.8
Mengmeng Zhang,Jingbo Liu,Yiding Yu,Wenshuo Ren,Rui Yuan,Xuanting Liu,Xiaomin Shang,Zhiyang Du,Meng-Lei Xu,Ting Zhang
Genistein (GEN) is the most abundant aglycone in soy isoflavones and is a key factor distinguishing soybeans from other food sources. It holds significant nutritional value and exhibits notable biological activity. However, the low water solubility of genistein limits its widespread application. To improve its water solubility, this study prepared γ-cyclodextrin (γ-CD)/GEN inclusion complexes and investigated their mechanism of action in alleviating hydrogen peroxide-induced oxidative stress damage in PC12 cells. The results showed that γ-CD/GEN could be uniformly and stably dispersed in the system. Cell experiments indicated that γ-CD/GEN significantly increased cell survival after treatment with organic hydrogen peroxide, and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of glutathione peroxidase 4 (GPx4). It also improved mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) levels, thereby alleviating cell damage caused by oxidative stress. Further Nrf2 knockdown experiments revealed that γ-CD/GEN failed to provide protection, and no protective effect was observed under RSL3 intervention. In conclusion, γ-CD/GEN exerts antioxidant effects by activating the Nrf2/GPx4 pathway, thereby reducing oxidative stress damage induced by organic hydrogen peroxide, providing a theoretical basis for the development of novel antioxidants.
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STEROIDS
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2019
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IF 2.136
Jiwang Tang,Jianxiu Wang,Liejiang Yuan,Yong Xiao,Xiu wang,Zong Yang
Dummy molecularly imprinted polymer microspheres (DMIPMS) towards estrogens were synthesized by Pickering emulsion polymerization employing genistein (GEN) as a dummy template molecule. The FTIR analysis indicated the successful preparation of the imprinted polymers, and the characterization results of scanning electron microscopy and nitrogen adsorption desorption measurement indicated that the obtained DMIPMS are in possess of regular spherical shapes, porous structures and narrow diameter distribution, a BET surface area of 402.74 m 2 g −1 , a total pore volume of 0.568 cm 3 g −1 and a pore diameter of 3.62 nm. The binding capacity and selectivity of DMIPMS were investigated in equilibrium binding experiments and chromatographic evaluation experiments through scatchard analysis and molecular imprinting factor (IF) analysis, respectively. The MIPs showed high binding capacity and excellent selectivity towards seven selected natural and synthetic estrogens, which are Estrone (E1), 17β-estradiol (βE2), estriol (E3), ethinylestradiol (EE2), dienestrol (DS), diethylstilbestrol (DES), and hexestrol (HEX). A method for selective determination of seven estrogens in milk samples via dummy molecularly imprinted solid phase extraction coupled with HPLC-MS/MS was developed, which showed good linearity from 2 to 500 µg L −1 with a correlation coefficient (R2) of more than 0.999. The detection limits were within the range of 0.10–0.35 µg L −1 and the recoveries of the seven estrogens at three spiking levels (10,100,250 µg L −1 ) ranged from 88.9% to 102.3% with relative standard deviation (RSD, n = 5) for intra-day and inter-day assays varied from 0.8% to 4.5%. The developed method is thus proven to be efficient and reliable for regular monitoring of trace estrogens in complex matrices such as milk samples.
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PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2025
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IF 4.7
Si-Yi Mo,Yuan Li,Ying-Ying Fan,Yao-Jun Zhang,Jing-Wen Liu,Xu-Tong Song,Xiao-Xiang Xu,Ye Cao,Jian-Qiu Jin,Qiu-Fei Xie
Background Temporomandibular disorder (TMD) pain is more prevalent in females than in males, with high estrogen levels potentially being a risk factor. Research indicates that 17β-estradiol (E2) exacerbates experimental occlusal interference (EOI)-induced orofacial hyperalgesia, which can be reversed by genistein. This study aimed to explore the central mechanisms within the spinal trigeminal nucleus (Sp5) related to the pain-exacerbating effect of E2 and the antiestrogenic properties of genistein in a model of EOI-induced chronic masseter pain. Methods Female rats underwent ovariectomy (OVX), followed by pretreatment with genistein or genistin (a control drug for genistein that does not inhibit protein tyrosine kinases (PTKs)), E2 replacement, and EOI application. The head withdrawal thresholds (HWTs) of the bilateral masseters were measured to evaluate pain sensitivity. Expression levels of p-ERK and two PTKs (Yes-associated protein, YAP; Src kinase, Src) in bilateral Sp5 were assessed through immunofluorescent staining and/or Western blotting. The ERK inhibitor PD98059 or vehicle was administered via intrathecal injection (i.t.) to inhibit the ERK1/2 signaling pathway. Results E2 intensified EOI-induced masseter mechanical hyperalgesia in OVX rats, and upregulated the phosphorylation of ERK1/2 in bilateral Sp5. Blocking phosphorylation of ERK1/2 in Sp5 reversed the exacerbating effect of E2. Genistein partially reversed the masseter hyperalgesia induced by E2 combined with EOI, possibly through the inhibition of PTKs and p-ERK1/2 upregulation in bilateral Sp5. Conclusion Genistein alleviates the pain-exacerbating effect of E2 on EOI-induced chronic mechanical hyperalgesia by inhibiting YAP and Src tyrosine kinases as well as the downstream ERK1/2 signaling pathway in Sp5.
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DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2025
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IF 1.9
Shuang Jin,Yubin Ren,Hongyao Cai,Biqiong Chen,Yupeng Cheng,Weili Liu,Cailiang Peng,Yujie Fu,Chen Lv,Huiling Li
This study introduces a green, sustainable, and efficient approach for biotransforming sophoricoside into genistein from Fructus Sophorae using Aspergillus oryzae by removal of one molecule of glucose by β-glucosidase, an edible microorganism immobilized on magnetic cellulose and treated with deep eutectic solvents (DES). The goal was to enhance the biotransformation ratio by optimizing reaction conditions and selecting the most suitable DES. Various DESs, including natural deep eutectic solvents (NADES), were assessed for their ability to improve catalytic performance. Among them, the NADES system comprising choline chloride (CHCL) and glycerol (G) exhibited the highest catalytic efficiency (32.19 mg/g) under optimal conditions: temperature 33 °C, time 65 hours, pH 5.5, and a liquid-to-solid ratio of 45:1 (mL/g). This yield was 10.60 times greater than the genistein yield from untreated F. Sophorae. This combination notably increased cell membrane permeability, aiding the bioconversion process. The cellulose immobilization technique provided a stable and reusable microreactor and maintained microbial activity (80.37%) over 10 cycles. These findings validate the bioconversion method as a promising and sustainable strategy for genistein production from plant-derived sophoricoside, with potential applications in pharmaceutical and nutraceutical industries.
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DOI
PubMed
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Liuming Xie,Ting Chen,Hong Li,Jindan Xiao,Linchun Wang,Soo-Ki Kim,Zhibing Huang,Jianhua Xie
Inflammatory bowel disease is a major health problem that can lead to prolonged damage to the digestive system. This study investigated the effects of an exopolysaccharide from genistein-stimulated Monascus purpureus (G-EMP) in a mouse model of colitis to clarify its molecular mechanisms and identified its structures. G-EMP (Mw = 56.4 kDa) was primarily consisted of → 4)-α-D-Galp-(1 →, → 2,6)-α-D-Glcp-(1→ and →2)-β-D-Manp-(1 → , with one of the branches being α-D-Manp-(1 →. G-EMP intervention reduced the loss of body weight, degree of colonic damage and shortening, disease activity index scores, and histopathology scores, while restoring goblet cell production and oxidative homeostasis, repairing colonic functions, and regulating inflammatory cytokines. RNA sequencing and Western blot analysis indicated that G-EMP exerts anti-inflammatory properties by suppressing the TLR4/MAPK/NF-κB inflammatory signaling pathway. G-EMP modulated the gut microbiota by improving its diversities, elevating the relative abundances of beneficial bacteria, declining the Firmicutes/Bacteroidota value, and regulating the level of short-chain fatty acids (SCFAs). Correlation analysis demonstrated strong links between SCFAs, gut microbiota, and the inflammatory response, indicating the potential of G-EMP to prevent colitis.
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DOI
PubMed
高影响因子
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