SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Chan Song,Haibo Liu,Linlin Zhang,Jie Wang,Chenglong Zhao,Qinyuan Xu,Cheng Yao
Nanozymes with high catalytic performance play a vital role in the construction of advanced biosensors for the signal detection and analysis of complicated biological samples. Herein, FeS nanoparticles (NPs) embedded in 2D carbon nanosheets (denoted as [email protected] ) are demonstrated to serve as excellent nanozymes with high catalytic activity . Notably, the introduction of mesoporous carbon substrate could effectively improve the enzymatic peroxidase-like catalytic ability of embedded FeS nanozymes, especially in the fluorescence catalytic system . Based on the high peroxidase-like activity of proposed [email protected] nanozymes, a facile method was then designed for H 2 O 2 assay with detection limit as low as 0.78 μM and 0.86 μM for the colorimetric and fluorescence method , respectively. The oxidation of the colorimetric and fluorescence substrates could be ascribed to the hydroxyl radical (·OH) produced by catalytic reaction of H 2 O 2 with [email protected] nanozymes. Thus, the [email protected] nanozymes-based H 2 O 2 sensing platform was further constructed to evaluate the antioxidant capacity of glutathione (GSH), cysteine (Cys), and gallic acid (GA). Notably, [email protected] nanozymes can keep the functionality in a broad pH range (2.0 ~ 9.0) for catalytic reaction, delivering a great potential in the fabrication of advanced biosensors.
研究领域:
生物化学
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纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jianan Li,Xinyong Li,Qidong Zhao,Zao Jiang,Moses Tadé,Shaobin Wang,Shaomin Liu
A novel photoelectrochemical (PEC) biosensing platform coupling with enzyme-induced biocatalytic precipitation (BCP) for H 2 O 2 was successfully constructed. TiO 2 nanotube arrays (TNs) were coated with polydopamine (PDA) and horseradish peroxidase (HRP) as the PEC electrode through a facile method. PDA was incorporated into the sensor system not only as a sensitizing agent to alter the optical properties of TiO 2 nanotube arrays and enhance the visible light (λ > 400 nm) absorption, but also as a bonding resin to HRP via Michael addition or Schiff base reaction. The immobilized HRP on the PEC electrodes could induce BCP reaction as the signal amplifier by oxidizing the 4-chloro-1-naphthol (4-CN) into insoluble products in the presence of H 2 O 2 . The quantitative monitoring of H 2 O 2 could be realized thanks to the photocurrent decrease caused by the BCP generated insoluble products deposited on the PEC electrode surface. The fabricated TNs@HRP/PDA PEC electrode for biosensing of H 2 O 2 exhibited a good sensitivity as well as selectivity in a detection range from 1 nM to 50 μM, with a detection limit of 0.7 nM.
研究领域:
生物传感
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材料科学
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分析化学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Jiaqi Xian,Yuhui Weng,Hantao Guo,Yan Li,Bixia Yao,Wen Weng
Iron-doped carbon nitride nanoparticles (Fe-CNNPs) were prepared from citric acid , urea and ferric chloride through a convenient one-pot solvothermal method . Oleic acid was used as the reaction medium. The morphology and chemical composition of the obtained Fe-CNNPs were characterized by multiple methods including transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). It is interesting to find that the Fe-CNNPs showed higher catalytic activity than horseradish peroxidase (HRP), and tetramethylbenzidine (TMB) can be catalytically oxidized in the presence of H 2 O 2 to produce a color change in aqueous solution. As H 2 O 2 can be generated in the oxidation process of glucose catalyzed by glucose oxidase (GOD), a novel sensitive method for the detection of glucose with a limit of detection (LOD) of 0.29 μM has been developed combined with the catalytic properties of GOD and Fe-CNNPs. The Fe-CNNPs with peroxidase mimetics activity may have potential applications in biotechnology field.
研究领域:
生物化学
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纳米材料科学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 37
·8599-8606
·
IF 5.344
Zhen Lin,Linlin Zheng,Wensong Yao,Shijun Liu,Yemei Bu,Qi Zeng,Xiaomin Zhang,Haohua Deng,Xinhua Lin,Wei Chen
Nanozymes have emerged as enzyme-mimicking nanomaterials to overcome the low stability and high cost of some natural enzymes. The design and fabrication of nanozymes with superior performance to natural enzymes are in urgent demand. Single-atom catalysts offer the unique characteristics of maximum atomic utilization, and are an excellent candidate for nanozymes. However, most of the reported synthesis methods for single-atom catalysts require the preparation of supports for single-atom catalysts in advance, which requires multiple steps and calcination in a high temperature atmosphere. Herein, Cu–N–C single-atom nanozymes (Cu–N–C SAzymes) were successfully designed via a one-pot solvothermal method. Cu–N–C SAzymes exhibited excellent peroxidase-mimicking activity that is superior to some other related nanoparticles. The mechanism study revealed that H2O2 was catalyzed by Cu–N–C SAzymes to generate reactive oxygen species. Furthermore, based on the excellent peroxidase-mimicking activity of the Cu–N–C SAzymes, a simple and sensitive detection method for H2O2 and glucose has been developed.
研究领域:
纳米技术
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催化
·
生物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 26
·14982-14989
·
IF 3.049
Pian Wu,Ping Ding,Xiaosheng Ye,Lei Li,Xiaoxiao He,Kemin Wang
Multimetallic nanomaterials have aroused special attention owing to the unique characteristics of chemical, optical and enhanced enzyme mimetic capabilities resulting from the synergistic effect of different metal elements. In this work, we present a facile, gentle, fast and one-pot method for preparing Cu/Au/Pt trimetallic nanoparticles (TNPs), which possess intrinsic and enhanced peroxidase-like activity as well as excellent stability, sustainable catalytic activity, and robustness to harsh environments. Kinetic analysis indicated that Cu/Au/Pt TNPs exhibited strong affinities with H2O2 and 3,3,5,5-tetramethylbenzidine (TMB) as the substrates. To investigate the feasibility of Cu/Au/Pt TNPs-based strategy in biological analysis, H2O2 was chosen as a model analyte and a sensitive and specific detection for H2O2 was acquired with a detection limit of 17 nM. By coupling with glucose oxidase (GOD), this assay could also achieve a sensitive and selective detection of glucose with a detection limit of 33 μM, indicating the versatility of the method. In view of the potential combination with diverse enzyme-related reactions, the Cu/Au/Pt TNPs-based strategy is promising as a universal platform for biosensors.
研究领域:
纳米生物学
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分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 32
·20838-20849
·
IF 4.036
Hanzhang Ye,Yongli Ding,Tingting Liu,Jiani Li,Qi Wang,Yuhao Li,Jingjing Gu,Zhanen Zhang,Xuedong Wang
Traditional bimetallic sulfide-based nanomaterials often have a small specific surface area (SSA), low dispersion, and poor conductivity, thereby limiting their wide applications in the nanozyme-catalytic field. To address the above issues, we herein integrated NiCo2S4 with N,S-rGO to fabricate a nanocomposite (NiCo2S4@N,S-rGO), which showed a stronger peroxidase–mimetic activity than its pristine components. The SSA (155.8 m2 g−1) of NiCo2S4@N,S-rGO increased by ∼2-fold compared to NiCo2S4 with a pore size of 7–9 nm, thus providing more active sites and charge transfer channels. Based on the Michaelis–Menten equation, the affinity of this nanocomposite increased 40% and 1.1∼10.6-fold compared with NiCo2S4 with N,S-rGO, respectively, highlighting the significant enhancement of the peroxidase-like activity. The enhanced activity of this nanocomposite is derived from the joint participation of ˙OH, ˙O2−, and photogenerated holes (h+), and was dominated by h+. To sum up, N,S-codoping, rich S-vacancies, and multi-valence states for this nanocomposite facilitate electron transfer and accelerate reaction processes. The nanocomposite-based colorimetric sensor gave low detection limits for H2O2 (12 μM) and glucose (0.3 μM). In comparison with the results detected by a common glucose meter, this sensor provided the relative recoveries across the range of 97.4–101.8%, demonstrating its high accuracy. Moreover, it exhibited excellent selectivity for glucose assay with little interference from common co-existing macromolecules/ions, as well as high reusability (>6 times). Collectively, the newly developed colorimetric sensor yields a promising methodology for practical applications in H2O2 and glucose detection with advantages of highly visual resolution, simple operation, convenient use, and satisfactory sensitivity.
研究领域:
生物化学
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分析化学
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Mengying Luo,Yinnan Song,Niaz Ali Khan,Wanru Wei,Ying Lu,Qinghua Zhao,Haiqing Jiang,Mufang Li,Dong Wang
H 2 O 2 sensor is widely used in biomedicine, environmental governance and food processing. However, it is limited by the complex preparation processes and high cost of raw materials. In this work, we develop a facile, simple and cost-effective method to fabricate H 2 O 2 sensor based on a screen-printed electrode via in-situ growth method. The copper was first electrodeposited on the working electrode of screen-printed electrode (SPE) as a sacrificial layer, subsequently, the SPE was immersed in horseradish peroxidase (HRP) containing phosphate buffer saline (PBS). The Cu ions formed by oxidation of the copper layer combined with HRP and phosphate ions to construct HRP-Cu 3 (PO 4 ) 2 hybrid nanoflowers on its surface. The fabrication process is straightforward, without complex reaction conditions and dangerous drugs, which is conducive to mass production. This sensor showed a good linear response to H 2 O 2 with a detection limit of 1.8 μM and a sensitivity of 263.3 μA∙mM −1 ∙cm −2 . Additionally, it possessed excellent selectivity and long-time stability. The performance of this sensor was investigated in various beverages such as milk, beer, and juice, showing reliable detection of H 2 O 2 which highlights the potential practical applications of the sensor.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Liling Wang,Yanbin Wang,Yifeng Zhou
Bimetallic MOF derivatives have shown excellent performance as nano-enzymes in the field of catalysis. Herein, PdCo oxide nanoflowers with three-dimensional flower were prepared by a simple pyrolysis method on a precursor of bimetallic PdCo-MOF. PdCoO x showed excellent peroxidase mimic activity, which could significantly promote the oxidation of TMB by H 2 O 2 . Compared with CoO x , the peroxidase mimic activity of the optimized PdCoO x -300 increased by 2.41-fold. PdCoO x -300 has high affinity for TMB and H 2 O 2 with K m values of 0.16 mM and 2.11 mM, which are only 57.03% and 36.87% of HRP, respectively. The highly specific peroxidase mimic activity is conducive to the sensitive detection of H 2 O 2 , glucose and ascorbic acid with limit of detection of 10, 100 and 10 nM, respectively. Furthermore, the total antioxidant capacity in the actual beverage samples was conducted, which showed good anti-interference ability and recovery rate.
研究领域:
催化
·
纳米酶研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Bing Bian,Qingyun Liu,Shitao Yu
Artificial nanoenzymes which can overcome some drawbacks of natural enzymes is a challenging topic in the biosensor field. Herein, we demonstrated 5, 10, 15, 20-tetrakis (4-carboxylpheyl)-porphyrin modified magnetic ZnFe 2 O 4 nanoparticles loaded on the surface of reduced graphene oxide (Por-ZnFe 2 O 4 /rGO), which exhibited intrinsic peroxidase-like activity and rapidly oxidized the peroxidase substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into a blue product (OxTMB) distinguished by naked eyes. Interestingly, by comparative study of different nanomaterials ZnFe 2 O 4 nanoparticles, ZnFe 2 O 4 /rGO and Por-ZnFe 2 O 4 , Por-ZnFe 2 O 4 /rGO was proved to possess the highest peroxidase-like activity. Electron spin resonance (ESR) verified the catalytic activity of Por-ZnFe 2 O 4 /rGO for H 2 O 2 was due to hydroxyl radical from decomposition of H 2 O 2 . Temperature and pH strongly affected the peroxidase-like activity of Por-ZnFe 2 O 4 /rGO nanocomposites. Under optimal conditions (pH = 4, 40 °C), the constructed sensor based on the catalytic activity of the Por-ZnFe 2 O 4 /rGO could be conveniently used for colorimetric detection of H 2 O 2 in the range of 0.7–30 μM with the detection limit of 0.54 μM. Moreover, the colorimetric sensor based on Por-ZnFe 2 O 4 /rGO exhibited a good linear response to glutathione (GHS) in the range of 2–40 μM with a low detection limit of 0.76 μM. The detection of GHS can be easily realized through the obvious color change by naked eyes without any complicated instrumentation.
研究领域:
生物传感器
·
纳米生物学
·
分析化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ruyu Li,Yu Zhao,Tingting Zhang,Zejin Ju,Xianbing Ji,Yanshuai Cui,Longgang Wang,Haiyan Xiao
The development of nanozymes using noble metal nanoparticles to replace natural peroxidase in bio-related detection has been gain great interest. Noble metal nanoparticles with small size have large specific surface area. However, small noble metal nanoparticles tend to aggregate without stabilizer. In this paper, small Pd nanoparticles (3–6 nm) stabilized by bitter gourd polysaccharide (Pd n -BGP NPs) were prepared by using bitter gourd polysaccharide as reducing agent and stabilizing agent. Pd 25 -BGP NPs had peroxidase-like catalytic property. And the catalytic kinetics of Pd 25 -BGP NPs towards substrates conformed to the Michaelis-Menten equation. Furthermore, a method was established to detect H 2 O 2 using Pd 25 -BGP NPs. The linear range and detection limit of this method was 20–320 μM and 2.04 μM, respectively. Finally, Pd 25 -BGP NPs had good biocompatibility when the concentration was less than 80 μg/mL. The prepared Pd nanoparticles with high stability showed their good prospect in H 2 O 2 detection.
研究领域:
纳米技术
·
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Yuhong Liu,Xuetao Zhang,Xinhua Shi,Xiaoping Jiang,Jing Zhang,Hui Wang,Meng Gao
Due to the important role of H 2 O 2 in human health and environment safety, the development of portable optical sensors for real-time detection of H 2 O 2 in gas phase is highly anticipated. Herein, an electrospun ratio fluorescence film (PVA@Dual-SiO 2 ) with bead structure was constructed by dual Aggregation-induced Emission (AIE) featured silica nanoparticles for convenient detection of gaseous H 2 O 2 . In this system, the blue-emitted AIE silica nanoparticles (T-SiO 2 ) with H 2 O 2 recognition capability was embedded in the spinning fiber as the fluorescent responder. Meanwhile, the red-emitted AIE silica nanoparticles (F-SiO 2 ) was designed as the fluorescent reference unit in film. By exploiting the peroxide-mediated reaction between T-SiO 2 and H 2 O 2 , the dual-emitting PVA@Dual-SiO 2 could sense gaseous H 2 O 2 with high sensitivity and selectivity by ratiometric fluorescence signals. It proved that this film could be used for detecting H 2 O 2 in food samples. This work developed a bead like fiber film sensor with dual AIE emission, which provided a representative case of the fabrication of portable and integrated film sensor with high fluorescence performance.
研究领域:
分析化学
·
生物传感器
·
材料科学
·
环境监测
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yu Xiao,Na Huang,Jiahui Wen,Dan Yang,Huanhuan Chen,Yijuan Long,Huzhi Zheng
Fluorescent bovine serum albumin-protected gold nanoclusters ( [email protected] NCs) can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxTMB for its peroxidase-like activity. The two absorption peaks of oxTMB overlapped with the excitation and emission peaks of [email protected] NCs, respectively, causing efficient quenching on the fluorescence of [email protected] NCs. The quenching mechanism can be attributed to the dual inner filter effect (IFE). Based on the dual IFE, [email protected] NCs were utilized as both peroxidase mimics and fluorescent reporters for H 2 O 2 detection and further for uric acid detection with uricase. Under optimal detection conditions, the method can be used to detect H 2 O 2 ranging 0.50–50 μM with a detection limit of 0.44 μM and UA ranging 0.50–50 μM with a detection limit of 0.39 μM. The established method had been successfully utilized for the determination of UA in human urine, with massive potential in biomedical applications.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）