查找使用阿拉丁产品的科研文献

Novel pH-Responsive CaO2@ZIF-67-HA-ADH Coating That Efficiently Enhances the Antimicrobial, Osteogenic, and Angiogenic Properties of Titanium Implants

Xinlin Li,Mengfei Xu,Zhaoli Geng,Xinyi Xu,Xu Han,Lin Chen,Ping Ji,Yi Liu

Titanium-based implants often lead to premature implant failure due to the lack of antimicrobial, osteogenic, and angiogenic properties. To this end, a new strategy was developed to fabricate CaO2@ZIF-67-HA-ADH coating on titanium surfaces by combining calcium peroxide (CaO2) nanoparticles, zeolite imidazolate framework-67 (ZIF-67), and the chemical coupling hyaluronic acid–adipic acid dihydrazide (HA-ADH). We characterized CaO2@ZIF-67-HA-ADH with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma–atomic emission spectrometry (ICP–AES). The results demonstrated that CaO2@ZIF-67-HA-ADH was pH-sensitive and decomposed rapidly under acidic conditions, and it released inclusions slowly under neutral conditions. Antibacterial experiments showed that the CaO2@ZIF-67-HA-ADH coating had excellent antibacterial properties and effectively killed methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PAO-1). Cell experiments revealed that the CaO2@ZIF-67-HA-ADH coating promoted pro-osteoblast adhesion, proliferation, and differentiation and also promoted the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs), exhibiting excellent osteogenic and angiogenic properties. In in vivo animal implantation experiments, the CaO2@ZIF-67-HA-ADH coating exhibited strong antimicrobial activity early after implantation and excellent osseointegration later after implantation. In conclusion, the pH-responsive CaO2@ZIF-67-HA-ADH coating conferred excellent antibacterial, osteogenic, and angiogenic properties to titanium implants, which effectively enhanced osseointegration of the implants and prevented bacterial infection; the coating shows promise for use in the treatment of bone defects.

Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis

Wenqian Zhang,Kangkang Zha,Yuan Xiong,Weixian Hu,Lang Chen,Ze Lin,Chenyan Yu,Wu Zhou,Faqi Cao,Hankun Hu,Bobin Mi,Guohui Liu

The diabetic wounds remain to be unsettled clinically, with chronic wounds characterized by drug-resistant bacterial infections, compromised angiogenesis and oxidative damage to the microenvironment. To ameliorate oxidative stress and applying antioxidant treatment in the wound site, we explore the function of folliculin-interacting protein 1 (FNIP1), a mitochondrial gatekeeper protein works to alter mitochondrial morphology, reduce oxidative phosphorylation and protect cells from unwarranted ROS accumulation. And our in vitro experiments showed the effects of FNIP1 in ameliorating oxidative stress and rescued impaired angiogenesis of HUVECs in high glucose environment. To realize the drug delivery and local regulation of FNIP1 in diabetic wound sites, a novel designed glucose-responsive HA-PBA-FA/EN106 hydrogel is introduced for improving diabetic wound healing. Due to the dynamic phenylboronate ester structure with a phenylboronic acid group between hyaluronic acid (HA) and phenylboronic acid (PBA), the hydrogel is able to realize a glucose-responsive release of drugs. Fulvic acid (FA) is added in the hydrogel, which not only severs as crosslinking agent but also provides antibacterial and anti-inflammatory abilities. Moreover, the release of FEM1b-FNIP1 axis inhibitor EN106 ameliorated oxidative stress and stimulated angiogenesis through FEM1b-FNIP1 axis regulation. These in vivo and in vitro results demonstrated that accelerated diabetic wounds repair with the use of the HA-PBA-FA/EN106 hydrogel, which may provide a promising strategy for chronic diabetic wound repair.

Synergistic effect of HA and BMP-2 mimicking peptide on the bioactivity of HA/PMMA bone cement

Zhenzhen Liu,Yong Tang,Ting Kang,Minyu Rao,Kejia Li,Qin Wang,Changyun Quan,Chao Zhang,Qing Jiang,Huiyong Shen

HA and bone morphogenetic protein-2 (BMP-2) possess superior osteoinductive and osteoconductive activities in the repair of bone defects. In this study, a BMP-2 mimicking oligopeptide (serine–serine–valine–proline–threonine, SSVPT) was introduced to the surface of nano-sized HA as the reinforcement phase of a poly(methyl methacrylate) (PMMA) cement, and the synergistic action of HA and oligopeptide on the bioactivity of HA/PMMA bone cement was investigated. Incorporated with 3-trimethoxysilyl propyl methacrylate (MPS)-modified HA, the compressive strength, flexural strength, and flexural modulus of HA/PMMA complied with the international standardization organization 5833 standard. Adherence measurement demonstrated that the introduction of HA and SSVPT could promote the adhesion and proliferation of human fetal osteoblast (hFOB) on the surface of HA/PMMA cement. The increased alkaline phosphatase (ALP) activity indicated that HA/PMMA could promote osteogenic differentiation of hFOB. All of the results illuminated that the HA/PMMA cement could be used as a bioactive material for regeneration and reconstruction of load-bearing bone.

Binding of methylmercury to humic acids (HA): Influence of solar radiation and sulfide addition reaction of HA

Hongwei Luo,Qianqian Cheng,Dongqin He,Ganning Zeng,Jianqiang Sun,Jun Li,Xiangliang Pan

Methylmercury (MeHg) is a neurotoxin that bioaccumulates in organisms and it forms strong complexes with reduced sulfur-containing ligands in dissolved organic matter (DOM). In the present study, the influences of solar radiation and sulfide addition reaction of humic acids (HA) on MeHg binding to HA were investigated using synchronous fluorescence and FT-IR two-dimensional correlation spectroscopic (2DCOS) analysis. Results showed that the complexation of fluorescent fractions of HA and sulfur-reacted HA (S-HA) with MeHg was not significantly affected by photoreaction treatments and the affinity of fluorescent fractions followed the order of protein-like fractions > humic-like fractions > fulvic-like fractions for both HA and S-HA. FT-IR 2DCOS analysis showed that the affinity of various binding sites in DOM for MeHg changed under different photoreaction treatments. Under dark treatment, small molecular compounds with low humification degree such as aromatic amino acids may be the site with the strongest binding ability to MeHg in HA, whereas aliphatic amino acids and sulfur-containing groups from sulfide addition reactions play a role in complexing of S-HA and MeHg. Under BS treatment (irradiation of DOM before MeHg binding), aliphatic compounds in HA preferentially bind to MeHg and aliphatic amino acids are the components with the strongest complexing ability; but for S-HA binding to MeHg, unsaturated functional groups and aromatic groups are more sensitive (alkenes > alkanes, phenols > alcohols). Under AS treatment (irradiation of DOM after MeHg binding), unsaturated bonds and aromatic compounds in HA preferentially bind to MeHg and aromatic amino acids show the strongest complexing ability; but for S-HA binding to MeHg, aliphatic groups show the strongest complexing ability (alkanes, alkenes > aromatics). These findings help us to better understand the complexation mechanisms between MeHg and DOM.

Bionic Mineralization Method on Designing and Preparation of CS/HA and CMCS/HA Composite Coating and Its Antibacterial Properties Study

Chenyang Xu,Wenzhu Li,Linyi Zhang,Jiakai Xu,Peng Han,Bin Chen

Poly(chitosan)/hydroxyapatite (CS/HA) and carboxymethyl poly(chitosan)/hydroxyapatite (CMCS/HA) composite coatings were successfully fabricated via biomimetic mineralization, with their structural characteristics and properties systematically analyzed. As the CS concentration increased during fabrication, the growth morphology of CS/HA coating transitioned from clustered to sheet-like structures, forming dense and uniform composite coating with strong substrate adhesion and excellent biocompatibility. The CMCS/HA coatings exhibited a coral-like structure comprising spherical particles, which ensured higher substrate coverage and demonstrated superior corrosion resistance. At 20 g/L, coatings prepared by mixing pre-calcified solution A with CMCS and then adding biomimetic mineralization solution A (defined as YOCS), or by mixing pre-calcified solution A with a mixture of biomimetic mineralization solution A and CMCS (defined as FOCS), both show optimum structure. Coatings incorporating 30 g/L CS or 20 g/L CMCS in the biomimetic mineralization solution exhibited optimum corrosion resistance, antibacterial activity, and biocompatibility.

Synthesis and properties of HA/ZnO/CNT nanocomposite

M. Ding,N. Sahebgharani,F. Musharavati,F. Jaber,E. Zalnezhad,G.H. Yoon

The goal of this study was to examine the tribomechanical properties of hydroxyapatite (HA)/ZnO and HA/ZnO/CNT composite ceramics (carbon nanotubes; with different ratios 0.5 wt%, 1.0 wt%, and 1.5 wt%). The composites were synthesized using the hydrothermal method in an autoclave. The structure and morphology of the composites were analyzed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX) and transmission electron microscope (TEM). The consolidation process was performed by sintering the compounds at 1150 °C under an argon gas atmosphere. The effects of ZnO and CNT on the mechanical properties and wear resistance of the HA-nanoparticle-based ceramic composites were investigated using a Vickers hardness tester, nanoindentation , and reciprocating wear tester equipment. The nanohardness and elastic modulus of the sintered samples increased and the friction coefficient of the sintered samples decreased as the fraction of CNTs increased compared to the pure HA and HA/ZnO compounds. Furthermore, the wear loss of HA/ZnO/CNT composites decreased with the increase in the CNT content compared to the HA and HA/ZnO samples.

A strong bioceramic microtube and its use in the fabrication of bioceramic implants through flexible deformation and pressure-assisted forming

Xueni Zhao,Zhaoyang Liu,Qihe Zhang,Zhipeng Zhu,Lingna Li

Bioceramic implants with complex structures, controllable pore size, and excellent mechanical property are hardly manufactured due to high hardness and brittleness of bioceramics. To address the challenge, the study developed a different way to facilitate the fabrication of a strong and tough hydroxyaptite (HA) microtube (HAMT) and ceramic implants. Ceramic implants such as HA bone cage and screw containing microtube were manufactured through flexible deformation and pressure-assisted forming. The compressive strength of HAMT formed by HA filament with 70 carbon fiber(CF) was 132.4±8.4 MPa, which was 73.68% higher compared to that of HA filament. Furthermore, the HA cage was closely stacked to form dense and uniform structure and had a better mechanical property. The HA bone screw based on the microtube had a compressive strength of 24.5±1.3 MPa and a pullout force of 83.5±1.4 N, which can be used as a bioabsorbable cancellous-bone screw.

Preparation and properties of Pue-loaded HA-ADH-PS nanomicelles

Huiru Wang,Yuanyuan Li,Yunpeng Min,Hang Zhang,Linkun Hao,Ru Zhang,Yunying Jiang,Yimin Song

3D printed biomimetic PEEK/HA scaffold induces cranial bone regeneration

Haojia Li,Luyang Liao,Yushuan Jia,Yingyuan Zhang,Kai Zhang,Xingdong Zhang,Xiangdong Zhu,Bo Yuan

The repair of critical-sized cranial defects resulting from trauma, congenital malformations, or surgery remains a significant clinical challenge. Polyetheretherketone (PEEK) is a commonly used material for cranial defect repair in clinical practice. However, its application is severely limited by drawbacks such as poor structural adaptability, bioinertness, and insufficient osseointegration, which significantly compromise clinical outcomes. In this study, we biomimicked the structure and composition of natural bone to fabricate a biomimetic PEEK/hydroxyapatite (HA) scaffold via 3D printing strategy. This scaffold exhibits a stochastic lattice structure and mechanical properties similar to natural bone, capable of sustained release of calcium and phosphate ions. An in vitro co-culture study with bone marrow mesenchymal stem cells (BMSCs) confirmed that the biomimetic scaffold promotes cell proliferation and enhances the expression of osteogenesis-related proteins and genes. In a rat model with a critical-sized cranial defect (8 mm diameter), the biomimetic PEEK/HA scaffold demonstrated excellent capability in inducing bone regeneration and promoting osseointegration. This research provides a viable strategy for the repair and functional reconstruction of clinical critical-sized cranial defects.

Walnut Shell-Porogen HA-MgO Porous Scaffolds for Bone Regeneration

Junjie Xiao,Pingfan Wu,Xing Ke,Lingyan Guo,Yu Li,Guoping Du,Linlin Chen

The regeneration of bone defects remains a significant clinical challenge in orthopedic and reconstructive medicine. Conventional approaches, such as autografts and allografts, often prove insufficient in addressing the multifaceted biological and mechanical demands essential for successful bone regeneration. Therefore, the design and development of bioactive porous scaffolds with improved regenerative potential have emerged as a critical focus of current research. In this study, a hydroxyapatite (HA)-based scaffold, commonly employed in bone regeneration, was innovatively engineered by incorporating magnesium oxide (MgO) and utilizing walnut shells as a natural pore-forming agent through high-temperature sintering. By adjusting the content ratios of MgO and walnut shells, HA-MgO porous bone tissue engineering scaffolds with varying proportions were successfully fabricated. The HA–2% MgO scaffold fabricated with a 50% walnut shell ratio demonstrated a well-defined porous architecture and compressive strength closely resembling that of human cancellous bone. In vitro evaluations further revealed improved biological performance, characterized by increased cell adhesion, proliferation, and migration, as well as significant osteogenic differentiation. These findings provide preliminary evidence that the synergistic presence of Ca 2+ and Mg 2+ ions can effectively stimulate bone regeneration, providing a strategic foundation for the rational design of next-generation HA-magnesium composite scaffolds.

Trace Cu(Ⅱ) can enhance the degradation of Orange Ⅱ in Fe(Ⅱ)/hydroxylamine/persulfate system

Jinpeng Wang,Ming Zhang,Runjuan Zhou,Jiyuan Li,Wei Zhao,Wenyuan Chen

This paper explored the effect of trace Cu(Ⅱ) (5 μM) on the degradation of Orange Ⅱ by Fe(Ⅱ)/Hydroxylamine (HA)/Persulfate (PS) system. The results showed that the addition of trace Cu(Ⅱ) not only improved the degradation efficiency of Fe(Ⅱ)/HA/PS system, but also broadened the pH range of Fe(Ⅱ)/HA/PS degradation Orange Ⅱ (2–10). Increasing the dosage of Fe(Ⅱ), Cu(Ⅱ) and PS will promote the degradation of Orange Ⅱ by the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. However, the optimal dosage of HA is 0.03 mM. Lower or excessive HA dosage is not conducive to the degradation of Orange Ⅱ by the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. Scavenger experiments show that SO 4 ·- is the main reactive oxygen species (ROS) in the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. In both acidic and alkaline environments, Cu(Ⅰ) reduced by hydroxylamine can promote the conversion of Fe(Ⅲ) to Fe(Ⅱ). In acidic environment, Fe(Ⅱ) reduced by Cu(Ⅰ) is the reason why trace Cu(Ⅱ) can enhance the efficiency of Fe(Ⅱ)/HA/PS system to degrade Orange Ⅱ. However, in alkaline environment, Cu(I) reduced by hydroxylamine is the main participant in the production of SO 4 ·- by activating persulfate, which is also the main reason for broadening the applicable pH of Fe(Ⅱ)/HA/PS for Orange Ⅱ degradation to alkaline environment.

Synthesis and Characterization of Photoresponsive Macromolecule for Biomedical Application

Pang Juan,Gao Ziyu,Zhang Long,Wang Huiming,Hu Xiaohong

Azobenzene, a photo switcher, has attracted increasing interest due to its structural response to photo stimulus in the field of information science and chemical sensing in the recent decades. However, limited water solubility and cytotoxicity restrained their applications in the biomedical field. In research, HA-AZO has been designed as a water soluble photo switcher in biomedical application. Synthesized HA-AZO had good water-solubility and a stable π-π* transition absorbance peak trans-isomer. With exposure to UV, transformation from trans-isomer to cis-isomer of HA-AZO could be realized according to UV spectra. Reversely, trans-isomer could be gradually recovered from cis-isomer in the dark. Simultaneously, quick response and slow recovery could be detected in the process of structural change. Moreover, repeated illumination was further used to detect the antifatigue property of HA-AZO, which showed no sign of fatigue during 20 circles. The influence of pH value on UV spectrum for HA-AZO was investigated in the work. Importantly, in acid solution, HA-AZO no longer showed any photoresponsive property. Additionally, the status of HA-AZO under the effect of UV light was investigated by DLS results and TEM image. Finally, in vitro cytotoxicity evaluations were performed to show the effects of photoresponsive macromolecule on cells.