JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Xueyun Wei,Lanrui Yang,Zhuomin Zhang,Gongke Li
Trace disinfection by-products (DBPs) in aquatic products may have serious impact on human health via direct or indirect consumption. However, the accurate and sensitive quantification of trace chlorophenol DBPs in complex aquatic products still remains a great challenge. In this work, the defective MIL-101(Fe)-NH 2 enrichment medium was prepared for developing a selective membrane solid phase extraction (M-SPE) technology for quantifying trace chlorophenol DBPs in fish, coupled with high performance liquid chromatography-ultraviolet-visible spectroscopy (HPLC-UV). The established analytical method showed good selectivity, sensitivity and accuracy, achieving the limits of detection from 0.03 to 0.15 ng/mL and the recovery from 80.0% to 117%, achieving sensitivity comparable to that of HPLC-mass spectrometry (MS)-based methods. The validated method was successfully applied to quantify chlorophenol DBPs in grass carp and crucian carp, offering a selective and sensitive alternative for monitoring chlorophenol DBPs in fish, with practical advantages for routine analysis via compatibility with widely available HPLC-UV.
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高影响因子
Analytical Methods
·
2012
·Vol. 5
·期 2
·536-540
·
IF 1.547
Jianfei Xiong,Xiangyu Tang,Guangming Zhou,Zhuo Guan,Limin Wu
A simple method was proposed for simultaneous determination of chlorpyrifos and its hydrolysis and biodegradation product 3,5,6-trichloro-2-pyridinol (TCP) in soil samples. The analytes were extracted with ethyl acetate (EtOAc) under optimized pretreatment conditions such as the type, volume and pH of extraction solvent. Clean-up of the extract from soil was conducted by dispersive solid phase extraction (DSPE) using activated carbon as the adsorbent. High performance liquid chromatography with ultraviolet detection (HPLC-UV) was used for simultaneous determination of the two analytes. Under the optimized HPLC conditions, the limits of detection (LOD), obtained on signal-to-noise ratio (S/N) of 3, for chlorpyrifos and TCP were 0.0080 mg kg−1 and 0.0120 mg kg−1, respectively. Linear calibration curves were obtained in the range of 0.05–2.00 mg kg−1 and 0.03–2.00 mg kg−1 for chlorpyrifos and TCP, respectively. The spike recoveries from soil ranged from 89.4% to 114%, and the relative standard deviation (RSD) was in the range of 0.80%–14%.
研究领域:
环境科学
·
分析化学
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Food Analytical Methods
·
2021
·Vol. 14
·期 7
·1345-1358
·
IF 3.366
Wei Yihua,Zhang Jinyan,Qiu Shuyan,Huang Qingqing,Yuan Linfeng,Chen Liumeng,Dai Tingcan,Tu Tianhua,Zhang Biaojin,Yan Han,Li Weihong
A simple, rapid, and accurate method for determination of selenocystine (SeCys 2 ), methyl selenocysteine (MeSeCys), selenourea (SeUr), selenite (Se(IV)), selenotmethionine (SeMet), and selenate (Se(VI)) in grain crops was developed, based on the coupling of ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) and ultraviolet irradiation hydride generation atomic fluorescence spectrometry (UV-HG-AFS). The conditions of IP-RP-HPLC-UV-HG -AFS were studied. The conditions of the pretreatment sample, such as types and dosage of proteinases, were investigated. The detection limits (LODs) for six Se species were between 0.77 and 1.77 μg·L −1 . Then, the proposed method has been successfully used to analyze selenium (Se) species in Se-enriched rice paddy ( Oryza sativa L. ), soybean ( Glycine max (Linn.) Merr. ), and sweet potato ( Ipomoea batatas (L.) Lam. ) with foliar application of sodium selenite solution. SeMet was the major Se spices in Se-enriched grain crops, ranged from 77–90% of the total Se contents. The capabilities of Se enrichment were relatively larger differences in three-grain corps as follows: soybean > rice paddy > sweet potato.
研究领域:
植物学
·
分析化学
·
营养学
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高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2014
·Vol. 28
·期 10
·1325-1334
·
IF 1.662
Zhangxiao Peng,Yan Wang,Xue Gu,Xiaojie Guo,Chao Yan
A method based on high-performance liquid chromatography coupled with ultraviolet detection was developed for studying the pharmacokinetics of costunolide (Cos) and dehydrocostus lactone (Dehy) in rats after intravenous (i.v.) administration. Following i.v. administration, the maximum plasma concentrations of Cos and Dehy were observed to be 12.29 ± 1.47 and 5.79 ± 0.13 µg/mL, respectively. The bioavailability of Cos was larger than that of Dehy; however, the clearance and the volume of distribution of Dehy were much larger than those of Cos. An ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry system with automated MS E (E represents collision energy) data analysis software (MetaboLynx TM ) was used to analyze and identify the metabolites of Cos and Dehy in vivo . Four metabolites of Cos and six metabolites of Dehy were discovered from the plasma, urine and feces of rats. The main metabolic pathway of Cos was phase II biotransformation, but the main metabolic pathways of Dehy was phase І biotransformation. Two sequential desaturations and N -acetylcysteine conjugation were the common metabolic pathways of Cos and Dehy in rats. This information may be useful for the further development of the two drug candidates. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
药理学
·
分析化学
·
药物代谢
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Zi-Yu Li,Jia-Hui Zhu,Yu-Zhuo Xu,Jie Zhao,Yu-Shen Liu,Lu-Liang Wang,Shu-Yang Sun,Shi-Lei Ji
Novel magnetic covalent organic frameworks (COFs) were prepared by one-pot synthetic strategy and employed as an efficient adsorbent for magnetic solid-phase extraction (MSPE) of naphthaleneacetic acid (NAA) in food samples. Depending on the predesigned the hydrogen bonding, π-π and hydrophobic interactions of magnetic COFs, the efficient and selective extraction process for NAA was achieved within 15 min. The magnetic COFs adsorbent combined with HPLC-UV was devoted to develop a novel quantitative method for NAA in complex food. The method afforded good coefficient in range of 0.002–10.0 µg mL -1 and low limit of detection was 0.0006 µg mL -1 . And the newly established method afforded less adsorbent consumption, wider linearity and lower LODs than the reported analytical methods. Ultimately, the method was successfully applied to determine NAA in fresh pear, tomato and peach juice. The magnetic COFs based MSPE coupled with HPLC-UV method provided a simple, efficient and dependable alternative to monitor trace NAA in food samples.
研究领域:
分析化学
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食品安全
·
材料科学
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Jian Gao,Jialong Wang,Jiejin Song,Yue Xing,Yuzhao Hou,Fengchao Wang,Jinghui Du,Jiang Wu,Liming Wang
The illegal addition of statins to tea-based products presents significant analytical challenges due to the complexity of the matrices involved. To address these challenges, we developed an ionic liquid-assisted magnetic solid-phase extraction (IL-MSPE) method coupled with HPLC-UV for the simultaneous determination of five statins. This protocol facilitates the rapid batch extraction of analytes within 6 min. The method demonstrated excellent linearity (R 2 ≥ 0.999, 0.1 to 20 μg/mL) and sensitivity, with limits of detection ranging from 0.022 to 0.054 μg/mL, and recovery rates between 89.2% and 117.5%. Density functional theory provided insights into a synergistic “dual-site” recognition mechanism, which is driven by π–π stacking, hydrogen bonding, and electrostatic interactions. Importantly, the method's compliance with green chemistry principles was validated by AGREE (0.77) and MoGAPI (90) metrics. Overall, this proposed workflow represents a reliable and high-throughput tool for monitoring unauthorized statin adulteration.
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高影响因子
Journal of Analytical Methods in Chemistry
·
2019
·
IF 1.589
Yong Zhang,Xian-Liang Zou,Yong-Li Wang,Lu Gao,Gui-Xin Chou
In this study, we used quantitative high-performance liquid chromatography equipped with an ultraviolet detector (HPLC-UV) and solid phase extraction (SPE) to determine the levels of four phenylethanoid glycosides and five triterpene acids in Liuwei Dihuang capsules (LDCs). LDCs were methanol-extracted and purified using a 500 mg/6 mL silica-based C18 SPE cartridge. Two elutions were analyzed on a ChromCore C18 column under two HPLC conditions. To improve the pretreatment clean-up, an array of silica- and polymer-based SPE cartridges were compared. Both wash and elution steps were also optimized to achieve the highest removal of impurities. Under optimal chromatographic conditions, good linearity was achieved for all compounds (correlation coefficient of r ≥ 0.999), with a quantification limit ranging from 0.0076 to 0.418 μg/mL. The method had satisfactory efficiency and reproducibility with recovery rates ranging from 91.6 to 99.3% with a relative standard deviation below 1.5%. Taken together, this demonstrated SPE as a suitable extension of HPLC-UV for the determination of phenylethanoid glycosides and triterpene acids in complex LDCs.
研究领域:
中药分析
·
药物化学
·
分析化学
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高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 1
·1-9
·
IF 6.232
Duo Huixiao,Li Yijing,Liang Xiaojing,Wang Shuai,Wang Licheng,Guo Yong
Magnetic hierarchical nickel/nickel oxide/carbon nanorods (Ni/NiO@C) were prepared via the pyrolysis of a metal-organic framework containing nickel(II) nickel (Ni-MOF; Ni 3 (BTC) 2 ) under argon atmosphere. In this material, magnetic Ni/NiO@C nanoparticles are embedded in porous carbon nanorods, and the morphology is similar to that of the original Ni-MOF precursor. The synthesized nanorods were applied as magnetic sorbents in the solid-phase extraction of five benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron), and their performance was evaluated under optimized conditions. The results show that the material exhibits high extraction recoveries from spiked samples (82.9%–107.6%) and linear response in the range of 0.2–450 μg·L −1 . It is also characterized by relatively low limits of detection (50–100 ng·mL −1 ) at a signal-to-noise ratio of 3. The sorbent is chemically stable and can be repeatedly recycled, with little decline in extraction capacity after 20 cycles of reuse. The method was successfully applied to the quantification of benzoylureas in tea, wolfberry, millet, and oat samples, and it showed high extraction efficiency.
Schematic representation of the synthesis of magnetic hierarchical nickel/nickel oxide/carbon nanorods derived from Ni-MOF. The material is employed as a sorbent for the magnetic solid-phase extraction of benzoylurea insecticides.
研究领域:
分析化学
·
环境科学
·
农药残留检测
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhiqin Ding,Mengdie Cai,Weimin Gan,Ping Yuan,Lijun Wei,Xianglei Cheng
In this paper, a novel, selective and sensitive high performance liquid chromatography-UV photolysis-fluorescence detection (HPLC-UV-FLD) method for nitrosamines (NAs) is proposed based on terbium-doped carbon dots (Tb-CDs). Experimental studies have shown that nitrosamines can be degraded by UV photolysis with wavelength at 254 nm to produce NO 2 − , while NO 2 − can rapidly and specifically quench the fluorescence of Tb-CDs. Based on this principle, N -nitrosodimethylamine (NDMA), Nnitrosomorpholine (NMor), N -nitrosopyrrolidine (NPYR), N -nitrosodiethylamine (NDEA) and N -nitrosopiperidine (NPIP) are separated within 8 min by HPLC. These five kinds of NAs showed a good linear relationship in the range of 10–2000 μg/L, and the limit of detection was as low as 2.25 μg/L. The proposed method is successfully applied to the detection of sweet potato, lotus root, potato, water chestnut, tomato and cucumber. The sample recoveries range from 77.35 % to 119.3 %.
研究领域:
食品科学
·
分析化学
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高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 9
·1857-1865
·
IF 2.366
Yang Fanfan,Xu Long,Liu Xiang,Chen Chen,Dias Alberto C. P.,Zhang Xiaoying
Abstract Bisphenol S (BPS) is a major alternative to Bisphenol A (BPA) newly applied in food packing material and disrupts reproductive and developmental endpoints through endocrine pathways. This study aimed to develop a rapid, sensitive, and accurate chemiluminescence immunoassay (CLIA) for the detection of BPS in canned beverage. Anti-BPS monoclonal antibody (mAb) was generated by immunizing Balb/c mice with immunogen (BPS–BSA conjugation). CLIA assay conditions, including 3-[9-(((3-( N -succinimidyloxycarboxypropyl) [4-methxylphenyl] sulfonyl) amine) carboxyl]-10-acridiniumyl)-1-propanesulfonate inner salt (NSP-SA-NHS) labeling ratio (1:40), antigen concentration (1 μg mL −1 ) and antibody titer (1:8000; 125 ng mL −1 in PBS (0.01 M, pH 7.4)) were determined, respectively. The half inhibition concentration (IC 50 ), detection limit (IC 10 ), and working range of the CLIA in canned beverage were 0.04, 1.48, and 0.32–5 ng mL −1 , respectively. Cross-reactivity with other bisphenols (4,4′-thiodiphenol, Bisphenol F, 4,4′- dihydroxy benzophenone, Bisphenol A and Bisphenol B) was less than 0.56%, and the recovery rates of BPS in canned beverage ranged from 80 to 109.2% (intra-assay) and from 82 to 108.4% (inter-assay). The results of CLIA were compared with high-performance liquid chromatography–UV (HPLC–UV) analysis, showing a good correlation of 0.956. After proper sample pre-treatment, this method allows relatively large amounts of sample analysis in a limited time period (30 min). Thus, it was deemed suitable and efficient for routine screening of BPS in canned beverage. Graphic abstract
研究领域:
分析化学
·
环境科学
·
食品安全
·
免疫学
·
毒理学
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高影响因子
TALANTA
·
2024
·
IF 5.6
Li Yang,Bolin Yu,Jie Yuan,Rongrong Xing,Runqin Wang,Xuan Chen,Shuang Hu
In this study, a novel technique utilizing vortex-assisted dispersive liquid-liquid microextraction with magnetic deep eutectic solvents (MDESs) was established and coupled with HPLC-UV to analyze six primary aromatic amines (PAAs). A novel hydrophobic MDES prepared from trioctylphosphine oxide , octanol , and CoCl 2 was used as the extractant, which could be dispersed uniformly during extraction, then floated onto the sample surface and re-aggregated into a single drop spontaneously after the extraction. The variables influencing the efficiency of the extraction process were investigated. When performing under the optimal extraction conditions, this method exhibited excellent linearity, low limits of detection (0.2–0.9 ng mL −1 ), and high precision (RSD ≤ 8.3 %). The enrichment factors ranged from 56 to 182. Satisfactory recoveries in the range of 91.6–109.2 % with RSDs < 7.1 % were obtained from three apple juices and three environmental water samples. The greenness and practicality of the developed method were assessed by AGREE, AGREEprep, and blue applicability grade index metric tools. Overall, the established procedure demonstrated its simplicity, speediness, environmental friendliness , and effectiveness in analyzing PAAs from aqueous matrices.
研究领域:
分析化学
·
环境科学
·
食品科学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Tianyu Zhou,Yanbo Wang,Tiantian Li,Hongji Li,Chunwei Yang,Dongshu Sun,Dandan Wang,Chunbo Liu,Guangbo Che
Constructing imprinting materials with excellent aqueous-recognition ability is a long-term challenge in imprinting fields. Hydrophilic molecularly imprinted materials have been capturing extensive attentions of material scientists, environmental experts and analytical chemists due to their excellent anti-matrix interference and aqueous-recognition ability. Herein, adopting resorcinol and melamine as double hydrophilic monomers, formaldehyde as hydrophilic crosslinker, 2,4,6-trichlorophenol as template and amino functionalized magnetic graphene oxide as substrate, a hydrophilic magnetic molecularly imprinted resin (MMIR) with enhanced adsorption and recognition performance was fabricated for the first time via integrating two-step template immobilization strategy with surface imprinting. The obtained MMIR was applied as magnetic solid phase extraction adsorbent to targeted separation of trace chlorophenols in complex environmental water samples combined with HPLC-UV detection. The mainly preparation conditions and extraction conditions were investigated in detail. Meanwhile, the physicochemical properties and isotherm/selective/kinetic adsorption were systematically explored. The MMIR exhibits excellent magnetic/hydrophilicity/selectivity/adsorptivity/reusability, and the established method presents low LODs (0.056–0.102 μg L -1 ), high recoveries (91–100%), wide linearity (0.5–100 μg L -1 , R 2 ≥ 0.991), excellent precision and accuracy for the five chlorophenols. The research provides a new sight on the development of eminent hydrophilic molecularly imprinted composites and the establishment of more efficient sample separation techniques for control/monitor/detection of target pollution in complex water environment.
研究领域:
材料科学
·
环境化学
·
分析化学
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高影响因子
危险品化学品经营许可证（带存储）