JOURNAL OF LUMINESCENCE
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2014
·
IF 2.367
Xing-Pei Ye,Shao-bo Sun,Ying-dong Li,Li-hua Zhi,Wei-na Wu,Yuan Wang
A new o -aminophenol-based fluorogenic chemosensor methyl 3,5-bis(( E )-(2-hydroxyphenylimino)methyl)-4-hydroxybenzoate 1 have been synthesized by Schiff base condensation of methyl 3,5-diformyl-4-hydroxybenzoate with o -aminophenol, which exhibits high selectivity and sensitivity toward Al 3+ . Fluorescence titration studies of receptors 1 with different metal cations in CH 3 OH medium showed highly selective and sensitive towards Al 3+ ions even in the presence of other commonly coexisting metal ions. The detection limit of Al 3+ ions is at the parts per billion level. Interestingly, the Al(III) complex of 1 offered a large Stokes shift (>120 nm), which can miximize the selfquenching effect. In addition, possible utilization of this receptor as bio-imaging fluorescent probe to detect Al 3+ in human cervical HeLa cancer cell lines was also investigated by confocal fluorescence microscopy .
研究领域:
生物化学
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分析化学
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细胞生物学
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DOI
高影响因子
Chemistry Central Journal
·
2018
·Vol. 12
·期 1
·1-7
·
IF 2.284
Jin Guofan,Xiao Fuyan,Liu Ruijiang
(Z,Z’)-1,1′-(4-ortho-Caboranyldimethyl)-bis(2-methoxyphenylethan-1-oxime) intermediate 3 was synthesized by a three-step reaction with a final treatment with base to give a new series of ortho-carboranyl biphenyloxime derivatives (4–8). Compounds 7 and 8 showed high solubility and the in vitro study results revealed high levels of accumulation in HeLa cells with higher cytotoxicity and boron uptake compared to l-boronphenylalanine.
研究领域:
药理学
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肿瘤学
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
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2015
·
IF 4.762
Jing Liu,Jian Liu,Weisheng Liu,Haoli Zhang,Zhengyin Yang,Baodui Wang,Fengjuan Chen,Haotai Chen
The combination of different fluorescent species into one nanostructure to develop fluorescent nanoparticles with multiple emission signatures by a single wavelength excitation has become a very popular research area in the field of multiplex bioanalysis, diagnostics, and multicolor imaging. However, these novel hybrids must be elaborately designed to ensure that the unique properties of each component are conveyed, i.e., fluorescent species and nanoparticles, and are maximized without serious interactions with each other. Herein, a first triple-fluorescence dumbbell nanoprobe with large Stokes shift based on incorporating fluorescein isothiocyanate (FITC) and lanthanide complexes onto Au–Fe3O4 NPs was synthesized. This hybrid displays well-resolved triple fluorescence emission, with FITC at 515 nm, Tb(III) complex at 545 nm, and Eu(III) complex at 616 nm under a single-excitation wavelength and is used for highly selective and sensitive colorimetric detection of Cu2+ with a detection limit of 30 nM. Under different Cu2+ concentrations, this hybrid exhibited distinguishable multiple colors under UV light, and the color could change in the presence of different concentrations of Cu2+. This sensor for ratio/multianalyte microscopic imaging of Cu2+ in HeLa cells and BHK cells was also demonstrated. Target molecules, such as folic acid, can be covalently attached to the fluorescent nanoparticle surface to serve as an effective probe for simultaneous multicolor imaging folate receptor-overexpressing HeLa cell lines in vitro.
研究领域:
生物分析
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诊断学
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成像技术
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Li-Ping Zong,Ling-Yu Ruan,Junji Li,Robert S. Marks,Jun-Song Wang,Serge Cosnier,Xue-Ji Zhang,Dan Shan
Enzyme mimetics have attracted wide interest due to their inherent enzyme-like activity and unique physicochemical properties , as well as promising applications in disease diagnosis, treatment and monitoring. Inspired by the attributes of nonheme iron enzymes , synthetic models were designed to mimic their capability and investigate the catalytic mechanisms . Herein, metal-organic gels (Fe-MOGs) with horseradish peroxidase (HRP) like Fe-N X structure were successfully synthesized though the coordination between iron and 1,10-phenanthroline-2,9-dicarboxylic acid (PDA) and exhibited excellent peroxidase-like activity. Its structure-activity relationship and the in-situ electrochemiluminescence (ECL) detection of H 2 O 2 secreted by Hela cells were further investigated. The highly dispersed Fe-N X active sites inside Fe-MOGs were able to catalyze the decomposition of H 2 O 2 into large amounts of reactive oxygen species (ROS) via a Fenton-like reaction under a low overpotential. Due to the accumulation of ROS free radicals , the luminol ECL emission was significantly amplified. A proof-of-concept biosensor was constructed with a detection limit as low as 2.2 nM and a wide linear range from 0.01 to 40 μM. As a novel metal organic gels based enzyme mimetic, Fe-MOGs show great promises in early cancer detection and pathological process monitoring.
研究领域:
生物化学
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生物技术
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分析化学
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材料科学
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癌症研究
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Pan Zhang,Hui Yang,Guojun Wang,Weijun Tong,Changyou Gao
The poly(allylamine hydrochloride)/trisodium citrate aggregates were fabricated and further covalently crosslinked via the coupling reaction of carboxylic sites on trisodium citrate with the amine groups on polyamine , onto which poly- L- lysine and hyaluronic acid were sequentially assembled, forming stable microspheres. The pH sensitive dye and pH insensitive dye were further labeled to enable the microspheres with pH sensing property. Moreover, these microspheres could be specifically targeted to HeLa tumor cells, since hyaluronic acid can specifically recognize and bind to CD44, a receptor overexpressed on many tumor cells. Quantitative pH measurement by confocal laser scanning microscopy demonstrated that the microspheres were internalized into HeLa cells, and accumulated in acidic compartments. By contrast, only a few microspheres were adhered on the NIH 3T3 cells surface. The microspheres with combined pH sensing property and targeting ability can enhance the insight understanding of the targeted drug vehicles trafficking after cellular internalization.
研究领域:
生物材料学
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肿瘤生物学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
·
2021
·
IF 3.247
Xiaoxiang Wang,Tao Lin,Wei Wu,Haisuo Wu,Dongdong Yan
The current investigation reports the synthesis of N-CDs using glucosamine, ascorbic acid, and ethylenediamine precursors by a simple hydrothermal technique. The formation of N-CDs was proved by various characterisation techniques such as X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier-Transform Infrared Spectrophotometer (FT-IR). The optical properties were investigated by fluorescence and UV-vis spectrophotometer. Also, N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. However, the metronidazole serves as an antibiotic against several microbial diseases but also a genotoxic, carcinogenic to the human when used in excessive dosage. The synthesised N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. Besides, the cytotoxicity of the N-CDs was studied to evaluate its toxicity against the HeLa cancer cells. It showed 65.6% cell viability and 34.3% toxicity against the cancerous cells, and similarly 71% of cells viability against H9C2 cells. Thus, the current investigation explores the promising selective sensing of N-CDs against MTZ, along with that, it proved its cytotoxicity against HeLa cancerous cells and non-toxicity against H9C2 cells. The synthesised CDs can be better MTZ sensors and anti-cancer agents on further development at the industrial scale.
研究领域:
材料科学
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分析化学
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毒理学
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DOI
高影响因子
RSC Advances
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2017
·Vol. 7
·期 57
·35687-35693
·
IF 3.108
Wenjie Gao,Yanhua Jiang,Zhihui Zhang,Yifang Zhang,Yanyan Liu,Yanhong Zhou,Xin Liu
Conventional protocols for cellular N-glycan profiling often need several time-consuming and labor-intensive steps, and a large amount of material (5–20 × 106 cells) is required. In this study, an optimized co-derivatization method was applied for convenient, rapid and highly-sensitive analysis of cellular N-glycan. Taking human cervical carcinoma cells (HeLa) as a model, the required amount for comprehensive cellular N-glycans analysis was reduced down to an original cell number of 105 and the total analysis time was decreased from several days to several hours. Good reproducibility of the method was also obtained with the CV averaging to less than 13.1%. In addition, compared to permethylation derivatization, more than 5-fold sensitivity improvement was achieved and more concise and clear mass spectra were obtained with the new developed method. As a preliminary study, this method was also successfully applied to reveal the difference in cellular N-glycan profiling of two heterogeneous live cell lines, human normal liver cells (L-02) and human hepatocyte carcinoma cells (HepG2), showing great potential for high-throughput analysis of N-glycans from limited cell samples, such as primary cell lines, cells obtained from cell sorting and small size cancer specimens.
研究领域:
生物化学
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分析化学
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医学研究
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DOI
高影响因子
Polymer Chemistry
·
2014
·Vol. 5
·期 12
·3824-3830
·
IF 5.368
Yan Zhang,Yujue Chen,Xing Li,Jibo Zhang,Jinlong Chen,Bin Xu,Xueqi Fu,Wenjing Tian
Fluorescent block amphiphilic copolymers are one of the most important bioimaging materials which are highly desirable for early stage cancer diagnosis and treatment. However, sometimes the application of the fluorescent block copolymers is severely limited because of the aggregation-caused quenching (ACQ) effect. In this study, new aggregation induced emission polymer dots (AIE Pdots) were prepared through a self-assembly process by using an AIE-conjugated block copolymer containing an AIE fluorophore, 9,10-bis(4-hydroxystyryl)anthracene, hydrophobic poly(ε-caprolactone) segments, hydrophilic poly(ethylene glycol) segments and folate groups. The AIE Pdots are monodispersed in H2O with an average diameter of 15 nm, and possess strong emission and high solid state fluorescence quantum efficiency (Φ = 27.0%). Furthermore, the AIE Pdots show good stability and little toxicity to living cells and thus can be utilized for targeted HeLa cell imaging. Biological imaging investigation indicated that the folic acid functionalized AIE Pdots can be applied for targeted HeLa intracellular imaging.
研究领域:
生物医学工程
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材料科学
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细胞生物学
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DOI
高影响因子
CHEMICAL RESEARCH IN TOXICOLOGY
·
2019
·
IF 3.274
Shuli Man,Haiyue Wang,Jin Zhou,Yingying Lu,Yanfang Su,Long Ma
The current study aimed to examine the anticancer activity of HTF-1, a cardiac glycoside (CG) isolated from Helleborus thibetanus Franch, using a cell-based model and to discover the underlying mechanisms with specific focus on autophagy. We found that HTF-1 was able to potently decrease the viability of several cancer cell lines especially for HeLa cervical carcinoma cells. It was discovered that HTF-1 dose dependently induced overproduction of ROS in HeLa cells, and the cell viability can be rescued when adding ROS scavenger N-acetyl-l-cysteine (NAC). More, we found that HTF-1 induced ROS-independent autophagy in concentration- and time-dependent manners in HeLa cells. This can be collectively verified by LC3-II and p62 abundance and also eGFP-LC3 puncta assay, bafilomycin clamp experiment, and acidotropic dye fluorescent labeling experiment. Additionally, TEM examination showed more autophagic vacuoles for HTF-1-treated HeLa cells. In HeLa cells, pretreatment with wortmannin (an inhibitor of the initial stages of autophagy to block autophagosome formation, thus, it should weaken the autophagy induction effect of HTF-1) decreased the autophagic flux and partially antagonized cell death induced by HTF-1, indicating that autophagy induced by HTF-1 played a cancer-suppressing role. Furthermore, coadministration of BAF (as a distal inhibitor of autophagy) with HTF-1 demonstrated a synergistic anticancer effect against HeLa cells. We believe that our work will enrich the understanding of CGs and especially anticarcinoma activity, also, pave the way for natural-product-based anticancer drug development.
研究领域:
药理学
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肿瘤学
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天然产物化学
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细胞生物学
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Lingzhi Zhao,Liu Zhao,Yanqing Miao,Chengxiao Zhang
A highly selective method for the electrochemical detection of reduced glutathione (GSH) has been developed on the basis of the product of a reaction between GSH and catechol as the electron mediator on single-walled carbon nanotubes modified glassy carbon electrode. A polymerization-adduct ECE mechanism at SWNTs/GCE was proposed. The oxidation peak potential was −0.160 V (vs. Ag/AgCl, sat. KCl) and the oxidation peak current showed a dependence relationship with GSH concentration in the range of 1.0 ∼500.0 μM with a detection limit of 0.5 μM GSH. The developed method can discriminate GSH from other thiol species such as L-cysteine (Cys) and cysteamine hydrochloride (Cym) within their certain concentration ranges. The as-established electrochemical method has been successfully applied for the determination of GSH from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis.
研究领域:
生物化学
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分析化学
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医学
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产品关联
DOI
高影响因子
ARCHIVES OF PHARMACAL RESEARCH
·
2016
·Vol. 39
·期 6
·762-770
·
IF 2.49
Wang Nan,Cui Cheng-Bin,Li Chang-Wei
A novel cyclic dipeptide, named penicimutide ( 1 ), and four known cyclic dipeptides, cyclo( l -Val- l -Pro) ( 2 ), cyclo( l -Ile- l -Pro) ( 3 ), cyclo( l -Leu- l -Pro) ( 4 ) and cyclo( l -Phe- l -Pro) ( 5 ), were isolated from a neomycin-resistant mutant of the marine-derived fungus Penicillium
purpurogenum G59. The structure of 1 , including the absolute configuration, was determined by spectroscopic and chemical methods, especially NMR and Marfey’s analysis. An unusual amino acid in 1 , 4,5-didehydro- l -leucine, was found for the first time occurring in nature. HPLC–ESI–MS analysis evidenced that 1 – 3 were produced only in the mutant strain, but 4 and 5 were produced in both the mutant and parental strains, indicating that the introduction of neomycin-resistance in the mutant activated pathways of 1 – 3 biosynthesis that were silent in the parental strain. Compound 1 selectively inhibited HeLa cells (among five tested human cancer cell lines) with an inhibition rate (IR %) of 39.4 % at 100 µg/mL, a similar inhibition intensity to that of the positive control 5-fluorouracil (IR % of 41.4 % at 100 µg/mL against HeLa cells). The present work exemplifies the effectiveness of our previous DMSO-mediated method for introducing drug-resistance in fungi to activate silent biosynthetic pathways to obtain new bioactive compounds.
研究领域:
微生物学
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药物化学
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肿瘤学
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DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Sheng Zhang,Niping Feng,Jinwen Huang,Minglong Wang,Lei Zhang,Junjie Yu,Xiling Dai,Jianguo Cao,Guozheng Huang
Aleuritopteris argentea (S. G. Gmél.) Fée is a medicinal fern consisting of an ent -labdane diterpene, i.e. alepterolic aicd, as the major metabolite. We recently isolated grams of alepterolic acid from A. argentea enabling subsequent structural modification. By incorporation of amino moiety to alepterolic acid, fifteen amide derivatives were synthesized, characterized, and further biological evaluated regarding their activity against four cancer cells and normal human liver cells. The potency of synthesized amides dramatically improved as compared to alepterolic aicd itself. The best hit (compound 11 ) inhibits HeLa cells with an IC 50 of 7.39 ± 0.80 μM, and is nearly nontoxic to normal cells. Compound 11 exhibits an inhibitory effect on the colony forming ability of the four cancer cells, especially of HeLa cells. Moreover, it induces apoptosis of HeLa cells by decreasing mitochondrial membrane potential and altering expression of apoptosis-associated proteins. Release of cytochrome c , activation of caspases-3, caspases-9 and alteration of Bax/Bcl-2 balance was detected in the biological assays. These results imply that compound 11 can inhibit the proliferation of cervical cancer cell line HeLa and induce apoptosis through the mitochondrial pathway. These findings encourage further rational structural modification of 15- carboxyl group of alepterolic acid.
研究领域:
药理学
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肿瘤学
·
有机合成化学
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产品关联
DOI
PubMed
高影响因子
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