Journal of Materials Chemistry B
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2015
·Vol. 4
·期 5
·910-919
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IF 4.726
Wei Gu,Qi Zhang,Ting Zhang,Yingying Li,Jian Xiang,Rui Peng,Jian Liu
Currently there is a great need to design nano-carriers which enable multi-modal imaging of tumors and administration of therapeutics with high efficacy simultaneously. Herein we report a facile and robust approach to fabricate multifunctional nano-capsules based on double emulsions. The hybrid nano-capsules were loaded with BSA capped gold nanoclusters (AuNCs) and indocyanine green (ICG) for dual-modal imaging and effective photothermal therapy. RGD peptides were conjugated onto the surface of the hybrid nano-capsules to target cells overexpressing integrin ανβ3. We demonstrated that the as-prepared nano-capsules can be used for both one-photon and two-photon fluorescence imaging of tumor cells, and subsequent photothermal ablation with high effectiveness. The background noise of tissue autofluorescence was dramatically suppressed in the two-photon imaging mode. Therefore, it assisted a great deal to acquire images with a high signal-to-noise ratio using the hybrid nano-capsules. Due to a unique combination of the one-photon/two-photon fluorescence imaging and highly-effective photothermal properties, the hybrid nano-capsules may find broad biomedical applications as attractive theranostic nanomaterials.
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ACTA PHARMACOLOGICA SINICA
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2016
·Vol. 37
·期 7
·941-949
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IF 3.166
Sun Hui-ping,Su Jing-han,Meng Qing-shuo,Yin Qi,Zhang Zhi-wen,Yu Hai-jun,Zhang Peng-cheng,Wang Si-ling,Li Ya-ping
Aim: To improve the therapeutic efficacy of cancer treatments, combinational therapies based on nanosized drug delivery system (NDDS) has been developed recently. In this study we designed a new NDDS loaded with an anti-metastatic drug silibinin and a photothermal agent indocyanine green (ICG), and investigated its effects on the growth and metastasis of breast cancer cells in vitro . Methods: Silibinin and ICG were self-assembled into PCL lipid nanoparticles (SIPNs). Their physical characteristics including the particle size, zeta potential, morphology and in vitro drug release were examined. 4T1 mammalian breast cancer cells were used to evaluate their cellular internalization, cytotoxicity, and their influences on wound healing, in vitro cell migration and invasion. Results: SIPNs showed a well-defined spherical shape with averaged size of 126.3±0.4 nm and zeta potential of −10.3±0.2 mV. NIR laser irradiation substantially increased the in vitro release of silibinin from the SIPNs (58.3% at the first 8 h, and 97.8% for the total release). Furthermore, NIR laser irradiation markedly increased the uptake of SIPNs into 4T1 cells. Under the NIR laser irradiation, both SIPNs and IPNs (PCL lipid nanoparticles loaded with ICG alone) caused dose-dependent ablation of 4T1 cells. The wound healing, migration and invasion experiments showed that SIPNs exposed to NIR laser irradiation exhibited dramatic in vitro anti-metastasis effects. Conclusion: SIPNs show temperature-sensitive drug release following NIR laser irradiation, which can inhibit the growth and metastasis of breast cancer cells in vitro .
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ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Wei Li,Hanbo Zhang,Xiaomeng Guo,Zuhua Wang,Fenfen Kong,Lihua Luo,Qingpo Li,Chunqi Zhu,Jie Yang,Yan Lou,Yongzhong Du,Jian You
Both photothermal therapy (PTT) and photodynamic therapy (PDT) are phototherapeutic approaches, which have been widely investigated for cancer therapy mediated by an external light source. Here, a nanosystem presenting the synchronous PTT and PDT effect realized through one-step near-infrared (NIR) light irradiation is reported. This system was fabricated by conjugating indocyanine green (ICG) on hollow gold nanospheres (HAuNS) using branched-polyethylenimine (PEI, MW = 10 kDa) as optimal linker, which provided a high ICG payload as well as a covering layer with suitable thickness on HAuNS to maintain ICG fluorescence and reactive oxygen species (ROS) productivity. The resulting system (ICG-PEI-HAuNS) had the molar ratio of ICG:PEI:Au = 3:0.33:5. Compared with free ICG, ICG-PEI-HAuNS exhibited dramatically enhanced stability of ICG molecules and greater intratumoral accumulation. The conjugation of ICG caused significantly higher plasmon absorption of ICG-PEI-HAuNS in the NIR region compared with HAuNS alone, inducing remarkably enhanced photothermal conversion efficiency and synchronous photodynamic effect under NIR light irradiation. Interestingly, compared with PTT or PDT alone, synchronous PTT and PDT produced by ICG-PEI-HAuNS upon NIR light irradiation induced significantly stronger antitumor and metastasis inhibition effects both in vitro and in vivo, which might be a promising strategy for cancer treatment.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Le Guo,Yi Zhou,Jiayi Ding,Jiabao Xiong,Lijun Zhu,Siyiti Amuti,Chi Zhang,Zhong Du,Xueliang Zhang,Biao Dong,Nuernisha Alifu
The integration of photothermal and near-infrared (NIR) imaging capabilities of indocyanine green (ICG) small molecules has attracted considerable attention in tumor diagnosis and treatment. However, the abnormal upregulation of cellular heat shock proteins (HSPs) induced by photothermal therapy (PTT) enhances cellular heat resistance, thereby severely affecting the efficacy of PTT. In this study, to address the impact of HSPs on the efficacy of PTT while obtaining high-quality NIR fluorescence imaging in the NIR region, we designed a targeted peptide@ICG nanofluorescent probe encapsulated in liposomes. The introduced cRGD targeting peptide not only possesses tumor-targeting capabilities but also features LA as the last amino acid in the targeting peptide, which can generate nitric oxide (NO) under reactive oxygen species (ROS) triggering. It can happen under 808 nm single-light source NIR light, and the guanidine group in the peptide decomposes and combines with singlet oxygen molecules to generate NO gas molecules, thereby exerting an elevated photothermal effect by inhibiting the expression of HSP70. In addition, the nanoprobes enable deep imaging and treatment of glioma in situ and can be combined with a laser speckle contrast imaging (LSCI) system for multimodal imaging. This composite probe demonstrates synergistic tumor therapeutic effects of photodynamic therapy (PDT), PTT, and gas therapy, offering a promising strategy for cancer treatment.
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MOLECULAR PHARMACEUTICS
·
2022
·
IF 5.364
Xinyu He,Huanhuan Chen,Shufang Chang,Shenyin Zhu
Rheumatoid arthritis (RA), a common chronic inflammatory joint disease with features of synovitis and pannus formation, may lead to irreparable joint damage and disability. Methotrexate (MTX) is known as the cornerstone of therapy for RA. However, the therapeutic effects of MTX are unsatisfactory due to its low retention in the inflammatory joints as well as systemic toxic effects. Fortunately, the use of multifunctional nanoparticles for diagnostics and in treatment shows potential for application as a strategy for traceable and targeted RA therapy. This research aims to develop novel nanoparticles that carry with perfluoropropane (PFP), indocyanine green (ICG), and MTX and investigate the corresponding enhancement in multimodal imaging both in vitro and in vivo. A modified double emulsion method was applied for the construction of encapsulated PFP-O2, ICG, and MTX (OIM@NPs), and the essential properties of the developed NPs were determined. The fluorescence and ultrasonic and photoacoustic imaging characteristics were experimentally evaluated both in in vitro and in vivo models. The OIM@NPs are stable and efficient nanoagents. They enable more targeted distribution in the inflammatory joints in RA rats. Moreover, the NPs play an important role as contrast agents for prominent ultrasound and photoacoustic imaging after laser and low-intensity focused ultrasound excitation, providing precision guidance and monitoring for subsequent treatment. This research may provide a novel and efficient strategy to better enable monitoring in inflammatory joints of RA patients and the developed NPs may be a promising nanoplatform for integrating multimodal image monitoring.
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RSC Advances
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2016
·Vol. 6
·期 19
·15460-15468
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IF 3.289
Chao Zhang,Tan Lu,Jingang Tao,Guang Wan,Hongxing Zhao
Herein, we have prepared PEGylated nano graphene oxide (NGO-PEG) that co-delivers paclitaxel (PTX) and indocyanine green (ICG) for tumor theranostics, integrating fluorescence imaging and chemotherapy. First, polyethylene glycol (PEG) with a dual-terminal amine was covalently conjugated with NGO to obtain the NGO-PEG. Furthermore, ICG, a near-infrared (NIR) Food and Drug Administration (FDA) approved fluorescent dye was covalently loaded onto NGO-PEG as a fluorescence contrast agent, NGO-PEG–ICG. PTX, a widely clinical used anti-cancer drug, was non-covalently immobilized onto the NGO surface via π–π stacking interaction and hydrogen bonding, giving the multifunctional nano-composite, NGO-PEG–ICG/PTX. The obtained nano-composite had great stability and biocompatibility. NGO-PEG showed (50 ± 2.1)% and (90 ± 1.6)% (w/w) of ICG and PTX loading ratios, respectively, which were determined from a UV-visible spectrometer. The fluorescence signal endowed by ICG was detected in the cytoplasm using confocal microscopy, demonstrating the high-efficiency human osteosarcoma (MG-63) cell uptake of NGO-PEG–ICG/PTX. Moreover, NGO-PEG–ICG/PTX could cause decreased cell viability compared to the same concentration of free PTX, revealing the excellent chemotherapeutic effect of NGO-PEG–ICG/PTX in vitro. In vivo fluorescence imaging results indicated a high MG-63 tumor accumulation of NGO-PEG–ICG/PTX, which could reach the maximum content in tumors at about 29.1% dose per g tissue at 24 h post injection with about eight days of tumor retention. Based on these encouraging results, the NGO-PEG–ICG/PTX was tail vein injected into tumor-bearing mice at day one and day eight, and the tumor-bearing mice showed complete tumor suppression or no relapse and treatment-induced major organ lesions over one-month of treatment. These results suggested that the synthesized versatile NGO-PEG–ICG/PTX with a high fluorescence contrast and chemotherapeutic effect can be a potential tumor theranostic nanoplatform.
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Journal of Biomaterials and Tissue Engineering
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2015
·Vol. 5
·期 5
·347-356
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IF 2.066
Chen Gong,Ding Jiali,Wu Haoan,Song Lina,Ma Ming,Lu Shanshan,Jiang Yanni,Luo Shouhua,Shi Yaxiang,Gu Ning,Zhang Yu
A kind of X-ray computed tomography (CT)/fluorescence dual-modal nanoemulsion probe that combined both CT imaging and near-infrared fluorescent (NIRF) imaging abilities was developed for application in tumor diagnosis. In this study, NIRF molecule indocyanine green (ICG-Der-01), a kind
of hydrophobic dye, was encapsulated in clinic CT contrast agent of iodinated oil injection based on oil-in-water nanoemulsion platform. The obtained ICG-iodinated oil nanoemulsion exhibited excellent CT and fluorescence capabilities. In vitro studies revealed that this nanoemulsion
could serve as an effective dual-modal probe by Micro-CT and confocal microscopy imaging of murine macrophage cells and mouse breast tumor cells without obvious cytotoxicity. Then in vivo clinical CT and NIRF imaging results suggested the nanoemulsion could accumulate in tumor by the
enhanced permeation and retention (EPR) effect and visualize tumor tissues in living body. All these results demonstrated the ICG-iodinated oil nanoemulsion had great potential in tumor diagnosis by dual-modal CT/fluorescence imaging in future.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Siyan Liang,Huan Liao,Guangyu Wu,Jingwen Zhong,Peiyuan Li
Skin and soft tissue infections (SSTIs) present a significant clinical challenge due to persistent bacterial colonization that exacerbates oxidative stress and impairs tissue repair. To address this, we developed a novel bilayer microneedle system (PI@Cur MN) combining polydopamine (PDA)-stabilized indocyanine green (ICG) at the tips with a Curcumin (Cur)-loaded backing layer, enabling synergistic photodynamic (PDT) and photothermal (PTT) antimicrobial therapy coupled with antioxidant functionality. The PDA-encapsulated ICG nanoparticles (PI) in microneedle tips demonstrated targeted near-infrared (NIR, 808 nm) responsiveness, achieving rapid bacterial eradication through concurrent PTT-induced hyperthermia and PDT-generated reactive oxygen species (ROS), with 99.89 % and 99.74 % reduction against Staphylococcus aureus and MRSA , respectively. Meanwhile, the backing layer containing curcumin remained on the skin surface as a drug reservoir. After the needle was inserted, it enabled a sustained release of Cur, which provided long-lasting antioxidant and anti-inflammatory effects. This multifunctional platform integrates multimodal antibacterial action, oxidative stress alleviation, and minimally invasive drug delivery, offering a clinically promising solution for managing SSTIs and promoting infected wound healing.
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International Journal of Nanomedicine
·
2021
·
IF 6.4
Shuai Li,Shuping Yang,Chong Liu,Jintong He,Tian Li,Changhui Fu,Xianwei Meng,Haibo Shao
Purpose P-glycoprotein (P-gp), which is highly expressed in liver cancer cells, is one of the obstacles for the treatment of cancer. In this study, we have prepared and characterized a kind of novel ICG&Cur@MoS2 (ICG and Cur represent indocyanine green and curcumin, respectively) nanoplatform, which can achieve photothermal-photodynamic therapy and inhibit the P-gp effectively and safely.Methods In this work, plenty of studies including drug release, acute toxicity, Western blot, real-time PCR, cell viability, therapeutic experiment in vivo, immunofluorescence and so on were conducted to test the antitumor potential of ICG&Cur@MoS2 and the inhibitory effect of curcumin on P-gp.Results The ICG&Cur@MoS2 NPs exhibit an excellent photothermal effect and relatively low toxicity. Cell viability in the ICG&Cur@MoS2 + NIR group was significantly lower than that in ICG@MoS2 + NIR group (75.3% vs 81.2%, 59.0% vs 64.4%, 20.3% vs 27.5%, and 15.4% vs 22.3%) at the concentration of ICG at 0.5, 5, 25, 50 μg/mL (P<0.05 at each concentration). Western blot, Q-PCR, and immunofluorescence assay indicate ICG&Cur@MoS2 NPs can inhibit the P-gp effectively and safely. In vivo, the tumors in the ICG@MoS2 + NIR group are significantly smaller than those in the MoS2 + NIR group (95.0 vs 420.9 mm3, p<0.05).Conclusion In conclusion, we have successfully synthesized ICG&Cur@MoS2 nanoparticles which can not only achieve PTT-PDT but also inhibit P-gp effectively. Our findings indicate that the PTT-PDT exhibits great potential in the treatment of hepatocellular carcinoma. Meanwhile, ICG&Cur@MoS2 can effectively inhibit the expression of P-gp, which will enhance the PDT effect.
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Pharmaceutics
·
2022
·Vol. 14
·期 10
·2047
·
IF 6.525
Yang Chen,Bin Wang,Wenping Chen,Tao Wang,Min Li,Zucheng Shen,Fang Wang,Jing Jia,Fenglan Li,Xiangyu Huang,Junyang Zhuang,Ning Li
Dihydroartemisinin (DHA), a widely used antimalarial agent, has clinical potential for the treatment of hepatic carcinoma. Although chemotherapy is indispensable for tumor therapy, it is generally limited by poor solubility, low efficiency, rapid clearance, and side effects. As an emerging treatment method, photothermal therapy (PTT) has many outstanding properties, but suffers from poor photostability of photosensitizer and incomplete ablation. Multimodal therapies could combine the advantages of different therapy methods to improve antitumor efficiency. Hence, we designed a nano-delivery system (ICG&[email protected]) using zeolitic imidazolate framework-8 (ZIF-8) with a high porous rate and pH sensitivity property, to co-load DHA and indocyanine green (ICG). Dynamic light scattering and transmission electron microscopy were used to characterize the prepared nanoparticles. The photothermal conversion and drug release performances of ICG&[email protected]were investigated. In vitro antitumor efficacy and cellular uptake were studied. The mechanism of the combination treatment was studied by reactive oxygen species level detection and western blot assays. In vivo antitumor assays were then studied with the guidance of ex vivo imaging. The results showed that the ICG&[email protected]based combination therapy could efficiently kill hepatic carcinoma cells and suppress tumor growth. This research provides a potential nanodrug for the treatment of hepatic carcinoma.Keywords:ZIF-8;nanoparticles;dihydroartemisinin;phototherapy;combination therapy
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Acta Biomaterialia
·
2021
·
IF 8.947
Kesi Wang,Linping Jiang,Liyan Qiu
L-arginine (L-Arg) is an important nitric oxide (NO) donor, and its exploration in NO gas therapy has received widespread attention. Application of nano-platforms that can efficiently deliver L-Arg and induce its rapid conversion to NO becomes a predominant strategy to achieve promising therapeutic effects in tumor treatment. Herein, an enhanced nano-vesicular system of ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild photothermal therapy (MPTT) was developed for cancer therapy. We integrated photosensitizer PEGylated indocyanine green (mPEG-ICG) into polyphosphazene PEP nano-vesicles through co-assembly and simultaneously encapsulated NO donor L-Arg into the vesicle center chambers to form mPEG-ICG/L-Arg co-loaded system IA-PEP. The unique nanostructure of vesicle provided considerable loading capacity for mPEG-ICG and L-Arg with 15.9% and 17.95% loading content, respectively, and efficiently prevented mPEG-ICG and L-Arg from leaking. Significantly, the reactive oxygen species (ROS) was produced by IA-PEP under 808 nm laser irradiation to perform PDT against tumors, which concurrently reacted with L-Arg to release NO and arouse gas therapy effectively. Moreover, the mild heat produced by IA-PEP could exhibit cooperative anti-tumor effect with minimal damage. As a consequence, in vivo antitumor investigation on nude mice bearing xenograft MCF-7 tumors verified the potent anti-tumor efficacy of IA-PEP under 808 nm laser irradiation with complete tumor elimination. Taken together, the IA-PEP nano-vesicle system designed in this work may provide a promising treatment paradigm for synergistic cancer treatment. Statement of significance Nitric oxide (NO) gas therapy has drawn widespread attention due to its “green” treatment paradigm with negligible side effects. L-arginine (L-Arg) is an important NO donor. However, how to efficiently deliver L-Arg and induce NO generation remains a big challenge since L-Arg is a water-soluble small molecule . Herein, we developed a nano-vesicle system IA-PEP to integrate photosensitizer PEGylated indocyanine green and L-Arg with high loading content and to produce a ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild-temperature photothermal therapy (MPTT) under 808 nm laser irradiation. The in vivo investigation on nude mice bearing xenograft MCF-7 tumors verified its potent anti-tumor efficacy with complete tumor elimination.
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Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 35
·5842-5849
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IF 4.872
Baoji Du,Xiaoxiao Gu,Wenjing Zhao,Zuojia Liu,Dan Li,Erkang Wang,Jin Wang
Phototherapy is widely studied with the development of photothermal nanomaterials. However, the laser power density for reaching effective phototherapy is still high and dangerous for normal tissues. In this research, based on multi-functional polydopamine, we proposed a general strategy to design diverse core–shell nanoparticles with different surface charges, and synthesized a targeted nanoplatform, AuNS@PDA–PEI–FA (APP), by coupling gold nanostar (AuNS) with polyethylenimine–folate (PEI–FA). Owing to the electrostatic interaction, the as-prepared APP can anchor a negatively-charged photosensitizer, indocyanine green (ICG), for photothermal and photodynamic synergistic cancer therapy. The APP–ICG is able to realize the targeted phototherapy for tumor cells even at the low power laser density of 0.33 W cm−2 both in vitro and in vivo. Furthermore, APP–ICG is not only a treatment nanoplatform but also a diagnosis agent. The integrative diagnosis results can be acquired by dual imaging technologies: the near-infrared fluorescence images and the photothermal images acquired by an in vivo imaging system and a thermal imaging camera, respectively. This new nanoplatform is a good diagnosis and treatment system and has the potential for further theranostics application.
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