CHINESE JOURNAL OF CHEMISTRY
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2024
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IF 5.5
Wenxin Wang,Jin Han,Yutong Zheng,Haijie Wang,Chengbiao Zhu,Yongyi Li,Yuqi Feng,Zhenwei Wei,Xiangyu Wang
Comprehensive Summary A monolithic column-based mass spectrometry (MS) analysis kit was prepared for whole blood analysis with MS. The kit is disposable and can be used for purification, storage, transportation and direct analysis of whole blood. The kit mainly consists of a capillary for quantitative microsampling, a cation exchange monolithic column for purification and storage, and a syringe for loading sample. This kit is very friendly to various users that one can easily siphon the blood in the kit followed by rapid clean-up. We established a quantitative method using the kit with a limit detection as low as 0.33 nmol/L, and achieved more than five orders of magnitude enhancement in sensitivity compared to direct nanoelectrospray ionization MS analysis. The column can avoid analyte exposure to environment, which helps the storage of the sample for laboratory analysis. The relative standard deviation of immediate blood analysis and storage blood analysis within 10 d was less than 10%. This method has been successfully applied to the quantitative analysis of procainamide hydrochloride in 2 μL rat blood. These results indicate that this disposable kit does have the potential to achieve highly sensitive quantitative MS analysis in biological samples, which is expected to become a cost-effective and powerful tool for in vitro diagnostics.
研究领域:
分析化学
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质谱学
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生物医学工程
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临床诊断
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Ziyu Gan,Jun Wang
Plants exhibit rapid responses to biotic and abiotic stresses by releasing a range of volatile organic compounds (VOCs). Monitoring changes in these VOCs holds the potential for the early detection of plant diseases. This study proposes a method for identifying late blight in potatoes based on the detection of (E) -2-hexenal, one of the major VOC markers released during plant infection by Phytophthora infestans . By combining the Michael addition reaction with cysteine-mediated etching of aggregation-induced emission gold nanoclusters (Au NCs), we have developed a portable hydrogel kit for on-site detection of (E) -2-hexenal. The Michael addition reaction between (E) -2-hexenal and cysteine effectively alleviates the etching of cysteine-mediated Au NCs, leading to a distinct fluorescence color change in the Au NCs, enabling a detection limit of 0.61 ppm. Utilizing the superior loading and diffusion characteristics of the three-dimensional structure of agarose hydrogel, our sensor demonstrated exceptional performance in terms of sensitivity, selectivity, reaction time, and ease of use. Moreover, quantitative measurement of (E) -2-hexenal was made easier by using ImageJ software to transform fluorescent images from the hydrogel kit into digital data. Such method was effectively used for the early detection of potato late blight. This study presents a low-cost, portable fluorescent analytical tool, offering a new avenue for on-site detection of plant diseases.
研究领域:
植物病理学
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分析化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
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2019
·Vol. 134
·期 1
·177-183
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IF 2.094
Xie Mingkun,Song Feng,Li Jienan,Xie Bowen,Wang Shuangshuang,Wang Weizhi,Ma Hong,Luo Haibo
Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.
研究领域:
法医学
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分子生物学
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遗传学
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
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IF 9.518
Deshuai Kong,Rui Jin,Tianshuang Wang,Hongxia Li,Xu Yan,Dandan Su,Caileng Wang,Fangmeng Liu,Peng Sun,Xiaomin Liu,Yuan Gao,Jian Ma,Xishuang Liang,Geyu Lu
Precise monitoring of pesticide with portable device was challenging because it required high sensitivity, short response time, strong stability and excellent selectivity. Herein, we newly constructed a stimuli-responsive hydrogel (SRHg)-based portable kit by embedding copper nanoparticles (CuNPs) in agarose hydrogel. In this work, dimethoate as inhibitor of urease restrained the generation of ammonia, which reduced in-situ etching of CuNPs, resulting in the fluorescence color response of test kit under ultraviolet illumination. Interestingly, by means of smartphone-based nanocolorimetry, the photo image of portable kit could be translated into digital information using ImageJ software, achieving a direct quantitative tool for dimethoate identification. The simplicity of SRHg-based portable kit combined with smartphone-based color recognition not only improved the analysis sensitivity (detection limit of 1.0 μg L −1 ), accuracy and stability, but also simplified operation process and shortened sample-to-answer analysis time (55 min), demonstrating that the methodology met the needs of daily testing and provided a new sight for on-site monitoring of food safety and human health.
研究领域:
分析化学
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食品安全
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环境科学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Lanlan Li,Xiaoyu Dong,Jihong Chen,Jing Xiao,Li Zeng,Zhengwen Huang,Zhirong Zou
A rapid and easy-used colorimetric method was developed for on-site and rapid analysis of Cr(VI). Under acidic condition, Cr(VI) was reacted with 1, 5-diphenylcarbazide (DPC) and a purple-red complex was formed subsequently, inducing the color changed from colorless to purple-red, this color variance can be detected by ultraviolet–visible spectrophotometer (UV–Vis), smartphone and even naked-eye. For realizing the goal of on-site analysis, this method was integrated in a three dimensional (3D) printed analytical kit, smartphone was used as a portable detector. Under the optimized conditions, limit of detections (LODs) of 0.0014 μg/mL and 0.0016 μg/mL were obtained by smartphone and UV–Vis, respectively. Concentration of Cr(VI) as low as 0.05 μg/mL was still visible by naked-eye. This colorimetric system was further applied to the analysis of several environmental water samples, recoveries of 90–107 % were obtained. It is a promising portable analytical device/system for Cr(VI) on-site and rapid analysis.
研究领域:
环境科学
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分析化学
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Hu Shengjie,Ma Yuchen,Fan Boyue,Miao Wenjun,Huang Rongrong
MicroRNAs (miRNAs) are post-transcriptional gene regulators and promising tumor biomarkers. Traditional detection methods are limited by high cost and specialized equipment. DNA-templated silver nanoclusters (DNA-AgNCs) are attractive fluorescent materials for biosensing but suffer from suboptimal brightness, with existing fluorescence enhancement strategies lacking universality. This study introduces a novel hydrogel kit integrating photonic crystals (PCs) and DNA-AgNCs for portable detection of miRNA-21. The formation of AgNCs within hybridization chain reaction (HCR) monomers generates a fluorescence signal, which is further amplified by PCs. Upon addition of miRNA-21, the conformational changes induced by miRNA-21 in the monomers trigger a cascade of hybridization events. Meanwhile, the formation of silver nanoclusters (AgNCs) is inhibited, thereby leading to an obvious decrease in fluorescence signal that can be distinguished by the naked eye. Hence, through the synergy of HCR-mediated target recognition, fluorogenic AgNCs-based transduction units, and PCs-triggered signal amplification, the hydrogel kit enhances the convenience and specificity of miRNA detection. In conclusion, this kit enables high-throughput fluorescence detection with a range of 1.56 to 200 nM. Moreover, it exhibits robust anti-interference capability in serum analysis, with recovery rates ranging from 91.84 to 102.74%. Owing to its strong matrix tolerance, the kit facilitates facile miRNA-21 detection, overcomes the drawbacks of conventional approaches, and holds considerable promise for clinical tumor diagnostic applications.
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Toxins
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2018
·Vol. 11
·期 1
·6
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IF 3.273
Ming Li,Mingna Sun,Xia Hong,Jinsheng Duan,Daolin Du
A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1limit of detection (LOD) in working buffer and a 200 ng g−1LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.Keywords:deoxynivalenol;contamination;survey;ELISA kit;agricultural productKey Contribution:A total of 328 agricultural product samples highly suspected to be contaminated were surveyed for deoxynivalenol using a self-assembly ELISA kit.
研究领域:
食品安全
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毒理学
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PubMed
高影响因子
FOOD CHEMISTRY
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2021
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IF 7.514
Yu-Ting Zhuang,Liuyimai Ma,Hao Huang,Lu Han,Li Wang,Yue Zhang
Accurate, sensitive, and selective analysis of acrylamide generated in thermally processed foods is of great significance for food safety. Herein, a novel acrylamide sensing platform is designed for both sensitive on-site colorimetric analysis and accurate UV–vis spectroscopy quantification, by integrating thiol-ene Michael addition with gold nanoparticles-mediated catalytical oxidation. The Michael addition reaction between acrylamide and glutathione efficiently alleviates glutathione-induced catalytic activity inhibition of gold nanoparticles, evoking the chromogenic reaction of H 2 O 2 -mediated 3,3′,5,5′-tetramethylbenzidine. With increasing the concentration of acrylamide, the oxidation of 3,3′,5,5′-tetramethylbenzidine is accelerated, presenting a series of shades from colorless to blue. The sensing platform exhibits excellent detection performance of acrylamide in the range of 0.5–175 μM with a detection limit of 0.16 μM, and is successfully employed in food samples. Especially, a portable assay kit based on the proposed platform is developed for visual determination of acrylamide, opening an avenue for smart sensors of food safety hazards.
研究领域:
食品科学
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分析化学
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纳米技术
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PubMed
高影响因子
Nanoscale
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2023
·Vol. 15
·期 8
·3991-3999
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IF 6.7
Lei Chen,Yun Gao,Jianxian Ge,Yi Zhou,Zhe Yang,Cang Li,Baoxing Huang,Kuan Lu,Dandan Kou,Dandan Zhou,Can Chen,Sixia Wang,Shuwang Wu,Jianfeng Zeng,Gang Huang,Mingyuan Gao
Magnetic resonance imaging (MRI)/nuclear medicine imaging (NMI) dual-modality imaging based on radiolabeled nanoparticles has been increasingly exploited for accurate diagnosis of tumor and cardiovascular diseases by virtue of high spatial resolution and high sensitivity. However, significant challenges exist in pursuing truly clinical applications, including massive preparation and rapid radiolabeling of nanoparticles. Herein, we report a clinically translatable kit for the convenient construction of MRI/NMI nanoprobes relying on the flow-synthesis and anchoring group-mediated radiolabeling (LAGMERAL) of iron oxide nanoparticles. First, homogeneous iron oxide nanoparticles with excellent performance were successfully obtained on a large scale by flow synthesis, followed by the surface anchoring of diphosphonate-polyethylene glycol (DP-PEG) to simultaneously render the underlying nanoparticles biocompatible and competent in robust labeling of radioactive metal ions. Moreover, to enable convenient and safe usage in clinics, the DP-PEG modified nanoparticle solution was freeze-dried and sterilized to make a radiolabeling kit followed by careful evaluations of its in vitro and in vivo performance and applicability. The results showed that 99mTc labeled nanoprobes are effectively obtained with a labeling yield of over 95% in 30 minutes after simply injecting Na[99mTcO4] solution into the kit. In addition, the Fe3O4 nanoparticles sealed in the kit can well stand long-term storage even for 300 days without deteriorating the colloidal stability and radiolabeling yield. Upon intravenous injection of the as-prepared radiolabeled nanoprobes, high-resolution vascular images of mice were obtained by vascular SPECT imaging and magnetic resonance angiography, demonstrating the promising clinical translational value of our radiolabeling kit.
研究领域:
医学影像学
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纳米医学
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放射化学
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PubMed
高影响因子
Forensic Sciences Research
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2022
·
IF 0
Qi Yang,Huan Yu,Yiling Qu,Xiaochun Zhang,Ruocheng Xia,Ziwei Wang,Rui Tan,Lei Xiong,Shihan Xi,Jun Wu,Yuzhen Gao,Suhua Zhang,Chengtao Li
Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East Asia, which do not overlap with the markers of Investigator® DIPplex kit. The typing system was named as GoldeneyeTM DNA ID System 35InDel Kit, and a series of developmental validation studies including repeatability/reproducibility, concordance, accuracy, sensitivity, stability, species specificity and population genetics were conducted on this kit. We confirmed that the 35InDel kit is precise, sensitive, species specific and robust for forensic practice. Moreover, the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture samples. All markers are proved to be highly polymorphic with an average observed heterozygosity (He) of 0.4582. The combined power of discrimination (CPD) is 0.999 999 999 999 978 and the combined power of exclusion in duos (CPED) and trios (CPET) are 0.978 837 and 0.999573, respectively, which are higher than those of the Investigator® DIPplex kit. Thus, the GoldeneyeTM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1945723 .
研究领域:
法医学
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群体遗传学
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人类学
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PubMed
Journal of Physical Chemistry C
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2026
·
IF 3.4
Jiaying Liu,Shiwei Wang,Dong Wang,Zhihao Yu,Lin Zhu,Chunzheng Wu,Hongbo Yu
Modifying noble metal catalysts with transition metal oxides (MOx) is an effective strategy to enhance both catalytic activity and selectivity through interfacial synergy. However, achieving precise spatial colocalization of the metal and oxide phases remains a challenge. Herein, Pd1-(CuOx)1 hybrid nanostructures confined within KIT-6 mesoporous silica were rationally synthesized via in situ oxidative transformation of PdCu bimetallic nanoparticles (NPs) under controlled thermal treatment. The resulting Pd1-(CuOx)1/KIT-6 catalysts exhibited markedly enhanced activity and chemoselectivity for the hydrogenation of acetophenone to 1-phenylethanol compared with monometallic Pd/KIT-6 and PdCu/KIT-6. The optimal catalyst with a Pd/Cu molar ratio of 1:1 achieved 93.1% acetophenone conversion and 98.2% selectivity toward 1-phenylethanol, and demonstrated broad applicability across a range of ketone substrates. Comprehensive structural characterization revealed that the Pd-CuOx interfacial sites are central to the observed performance enhancement: metallic Pd facilitates H2 dissociation, while adjacent CuOx species are proposed to facilitate carbonyl adsorption, contributing to the overall synergistic enhancement. This interface-driven synergistic mechanism provides a general, efficient, and cost-effective strategy for the selective hydrogenation of multifunctional molecules.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Minghui Kong,Yang Lu,Yuan Ma,Xu Zhao,Jiahang Wu,Geyu Lu,Xu Yan,Xiaomin Liu
On-site quantitative analysis of pesticide residues is crucial for monitoring environmental quality and ensuring food safety. Herein, we have developed a reliable hydrogel portable kit using NaYbF 4 @NaYF 4 : Yb, Tm upconversion nanoparticles (UCNPs) combined with MnO 2 nanoflakes. This portable kit is integrated with a smartphone reader and Python-assisted analysis platform to enable sample-to-result analysis for chlorpyrifos. The novel UCNPs maximizes energy donation to MnO 2 acceptor by employing 100 % of activator Yb 3+ in the nucleus for NIR excitation energy collection and confining emitter Tm 3+ to the surface layer to shorten energy transfer distance. Under NIR excitation, efficient quenching of upconversion blue-violet emission by MnO 2 nanoflakes occurs, and the quenched emission is recovered with acetylcholinesterase-mediated reactions. This process allows for the determination of chlorpyrifos by inhibiting enzymatic activity. The UCNPs/MnO 2 were embedded to fabricate a hydrogel portable kit, the blue-violet emission images captured by smartphone were converted into corresponding gray values by Python-assisted superiority chart algorithm which achieves a real-time rapid quantitative analysis of chlorpyrifos with a detection limit of 0.17 ng mL −1 . At the same time, pseudo-color images were also added by Python in “one run” to distinguish images clearly. This sensor detection with Python-assisted analysis platform provides a new perspective on pesticide monitoring and broadens the application prospects in bioanalysis.
研究领域:
环境科学
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食品安全
·
生物分析化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）