JOURNAL OF APPLIED ENTOMOLOGY
·
2025
·Vol. 149
·期 7
·1090-1104
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IF 2
Arushi Mahajan,Meena Devi,Pallvi Mehra,Amarjeet Kaur,Sanehdeep Kaur
Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae), known for its ravenous feeding habits, is a polyphagous pest with a high reproductive rate, often developing resistance to most of the conventional insecticides, posing a substantial pest management challenge. Chlorantraniliprole (CTPR) is an anthranilic diamide insecticide, which is widely used against lepidopteran pests, including S. litura . Understanding the physiological and biochemical alterations associated with CTPR resistance is crucial for effective resistance management. Our research aimed to investigate the overall impact of CTPR on nutritional parameters, and biochemical and immunological responses in laboratory-selected susceptible (Unsel-Lab) and resistant (CTPR-Sel) S. litura populations. Results showed a reduction in nutritional parameters and digestive enzyme activity, with a more pronounced decline in the CTPR-Sel population. In contrast, detoxification enzyme activities (MFO, GST, EST, ACP and AKP), along with AChE, were significantly elevated, suggesting an adaptive response to CTPR exposure, with the CTPR-Sel population exhibiting a stronger detoxification capacity. Antioxidant enzyme assay indicated increased SOD activity but decreased CAT activity in both populations, reflecting oxidative stress. Immune response markers, including total haemocyte count, lysozyme and phenoloxidase activity, showed significant variations between the two populations, suggesting differential immune-physiological adaptations. These findings highlight the physiological trade-offs associated with CTPR resistance in S. litura and emphasise the necessity of integrated pest management strategies to mitigate resistance development.
研究领域:
昆虫学
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农药毒理学
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昆虫生理学
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化学生态学
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综合害虫管理
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Xianyou Sun,Zihan Jin,Shaoxiang Zhang,Peng Lin,Xueming Zhang,Jiaying Sun,Yanchi Zhang,Yong Zhou,Yanjie Hu,Zhuoru Huang,Jiahao Hu,Shichao Tian,Guangqing Ren,Ping Wang,Liubing Kong,Hao Wan
Urine, abundant in disease-related biomarkers, offers advantages such as easy collection and noninvasiveness. Portable biochemical sensors are ideal for efficient on-site applications due to their flexibility and accessibility. However, existing sensors face challenges in complex sample operations and limited sensor renewal, hindering large-scale and convenient urine analysis. This study presents the “lab on pipette” (LOP) concept to address these issues, enabling efficient on-site urine analysis. The LOP system integrates a multifunctional pipette tip and a miniaturized system, facilitating the detection of multiple biomarkers in urine. Laser-induced graphene (LIG) technology fabricates an electrochemical electrode array on flexible polyimide, and custom-designed electrical interfaces ensure quick plug-and-play connections. Gold nano dendrites, Prussian blue (PB), and nickel hexacyanoferrate (NiHCF) form a multilayer functional interface, enabling highly sensitive detection of pH, hydrogen peroxide, glucose, and ascorbic acid with pH compensation. NiHCF doping enhances PB stability, while nanoalumina-doped porous enzyme membranes improve sensor repeatability and reliability. Gold nano dendrites anchor the enzyme membrane on curved surfaces, ensuring robust performance. The LOP achieves detection limits of 7.19 and 8.57 μM for glucose and ascorbic acid, respectively. Its low cost, disposability, portability, and reliability highlight its potential for on-site biochemical detection and personalized health monitoring.
研究领域:
生物医学工程
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传感技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Shenguang Ge,Yan Zhang,Lina Zhang,Linlin Liang,Haiyun Liu,Mei Yan,Jiadong Huang,Jinghua Yu
A trimetallic dendritic Au@PdPt nanparticles (Au@PdPt NPs) with intrinsic peroxidase-like catalytic activity were fabricated by a facile method. Au@PdPt NPs catalyzed the reduction of H 2 O 2 with the help of the electron mediator (thionine), which produced the electrochemical signals dramatically amplified. A sandwiched sensor was designed and implemented in the paper chip device for the determination of K-562 cells. The surface of dendritic Au@PtPd NPs was linked with folic acid by click chemistry. Folic acid was attached to the folate receptor (FR) of cell surface with high affinity. So, K-562 cells could be assayed based on folic acid-functionalized Au@PtPd NPs. On the other hand, folic acid-functionalized Au-paper working electrode on origami electrochemical device was fabricated, which could be used to capture K-562 cells. The twice utilization of folic acid for the capture of K-562 cells as well as the attachment of dendritic Au@PtPd NPs nanoprobe, which enhanced the selectivity of sensor for the determination of cells. The proposed biosensor exhibited excellent analytical performance toward the cytosensing of K562 cells with a wide detection linear range from 1.0 × 10 2 to 2.0 × 10 7 cells mL −1 and a detection limit of 31 cells mL −1 . Further, the proposed electrochemical sensor was applied to monitor cells. Au@PtPd NPs nonenzymatic catalysts could be used as signal-amplifying nanoprobes for ultrasensitive electrochemical sensing.
研究领域:
生物医学工程
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分析化学
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纳米技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Liuyue Xiao,Tianhao Li,Hui Ling,Yanjing Yang,Shian Zhong
The hybrid ratiometric fluorescence sensors (GKHMIPs & RKHMIPs) encompass luminescent substances incorporated within a core-shell architecture. These sensors manifest a diverse array of hues in response to variations in the concentration of alpha-fetoprotein, accomplished through disparities in the quantum dots (QDs)-protein response. This study is devoted to comprehending the principles governing the alteration of fluorescence during the recognition process of the ratiometric fluorescence sensor. A compensation mechanism, rooted in the correlation between fluorescence and protein response, is postulated through a fluorescence interaction model (multi-FRET model). Multi-FRET model can be divided into FRET process and Fluorescence compensate process (FCP), FCP occurs when electron orbits are filled, hindering the fluorescence resonance energy transfer process and leading to the return of excited state electrons to the ground state by alternative pathways. Based on the multi-FRET model we demonstrate the Sherpa formular to calculating the fluorescence-target relation. To assess the visual impact, the CIE-Lab model is introduced to validate the rationale behind the multi-color colorimetric model. The influence of different mixing ratios on the visualization effect is elucidated using the concentration fluorescence response model, and the optimal relationship between wavelength combinations in the tricolor mixing model is proposed.
研究领域:
生物传感器
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分析化学
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分子生物学
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材料科学
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产品关联
DOI
高影响因子
CURRENT MICROBIOLOGY
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2025
·Vol. 82
·期 9
·1-11
·
IF 2.6
Gu Yiwen,Lin Xiu,Lu Yanxu,Fang Shuguang,Hu Kun,Ju Xin,Li Liangzhi,Chen Zhi
The increasing use of antibiotics in shrimp aquaculture has raised concerns regarding ecological safety and the emergence of antibiotic resistance. This study aimed to isolate and characterize probiotic lactic acid bacteria (LAB) strains from the gut of Penaeus vannamei to evaluate their potential as antibiotic alternatives in aquaculture. Two strains, S1 and S2, were identified with 99.79 and 99.51% similarity to Pediococcus acidilactici DSM 20284 and Enterococcus lactis BT159, respectively. Based on minimum inhibitory concentration (MIC) assays, both strains exhibited high sensitivity to the tested antibiotics. They also demonstrated notable acid and bile salt tolerance, with S1 maintaining a survival rate of 30% at pH 5 and S2 achieving 40% survival at pH 6. Under 0.03% bile salt concentration, survival rates were approximately 60% (S1) and 45% (S2), while S1 retained 15% viability even at 0.9% bile salt. Enzymatic activity analysis indicated that both strains exhibited lower amylase activity compared to other hydrolytic enzymes, with S2 showing higher cellulase and protease activities than S1. Antimicrobial assays revealed that both strains exerted broad-spectrum inhibition against four indicator pathogens. S1 exhibited the strongest inhibitory effect against Aeromonas hydrophila (inhibition zone: 1.6–2.2 cm), whereas S2 showed the highest inhibition against Vibrio parahaemolyticus (1.5–2.4 cm). Feeding trials demonstrated that the combined application of S1 and S2 significantly enhanced shrimp body weight (6.05 ± 0.09 g vs. 9.23 ± 0.2 g, P < 0.01) and survival rate (62.49% ± 1.56 vs. 93.78% ± 2.55, P < 0.01). These findings highlight the potential of Lactobacillus strains as probiotics in shrimp farming, offering a sustainable approach to reducing antibiotic dependency and improving shrimp health and productivity.
研究领域:
水产养殖
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微生物学
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应用微生物学
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益生菌研究
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抗生素耐药性
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海洋生物学
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动物营养
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 55
·44714-44721
·
IF 3.84
Siqi Zhang,Kun Wang,Zhenyu Li,Zhongmin Feng,Ting Sun
In this work, a one-pot signal amplified strategy was constructed based on generating a hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme as the color product for the detection of DNA sequences. A DNA sequence associated with the hepatitis B virus (HBV) was selected as a model target. The enzyme-free and label-free biosensor contained three oligonucleotide terms as target DNA, hairpin structures H1 and H2 which were partially complementary. It should be noted that the G-quadruplex structure was partially hidden in the hairpin structure H2. In the absence of THBV, hairpins H1 and H2 were stable enough due to the complementary sequences at the end of the oligodeoxynucleotides, which made it difficult to complete the self-assembly. Upon addition of THBV, self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to facilitate H1–H2 complex formation and releases THBV to the next cycle. In the presence of hemin and K+, the G-quadruplex at the end of the H1–H2 complex was liberated to form a hemin/G-quadruplex structure, which could catalyze achromatous tetramethylbenzidine (TMB) into a colored product. The color change of the solution could be quantitated by spectrophotometry and the naked eye. We also employed 20% ethanol in the buffer to accelerate H1–H2 complex formation in the DNA strand displacement reaction (DSDR), which cut down the reaction time of the detection process from 12 h to 1 h. The detection limit of the colorimetric sensor is 9.5 pM, and the linear range is 50 pM to 100 nM.
研究领域:
生物化学
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分子生物学
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分析化学
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医学诊断
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DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2021
·
IF 6.141
Lijie Liu,Jinhong Li,Yong Deng,Zhiwei Yang,Kaiyue Huang,Siyi Zhao
Phase change materials with an ability to store the renewable thermal energy find widespread applications as building materials and temperature control devices. In this study, a lightweight and energy-efficient temperature regulating material based on the sandwich structure of Na 2 SO 4 ·10H 2 O, expanded vermiculite and magnesium oxychloride cement has been reported. The composite material responded in the range 20–40 °C and extended the heating and cooling period of the indoor temperature. The developed material exhibited an improved application performance as compared to the traditional heat preservation materials. Further, the supercooling of Na 2 SO 4 ·10H 2 O/expanded vermiculite was determined to be 1.20 °C, along with shape stability. Different synthesis methods and phase change materials with cement were explored to minimize the degradation of the thermal regulation performance, and the sandwich structure was observed to exhibit the optimal performance. Owing to the low thermal conductivity of Na 2 SO 4 ·10H 2 O/expanded vermiculite and magnesium oxychloride cement, the extended holding time of the indoor temperature was observed. The developed material exhibits significant potential for future applications as self-regulating materials and novel temperature control devices in which the optimal thermo-physical properties are desirable.
研究领域:
建筑材料
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能源材料
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热物理工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Xiaoqing Lin,Longqian Xu,Zhenyu Zhao,Shanshan Liu,Jiaming Zhang,Deli Wu
Low electron-transfer efficiency and cumbersome processes are widely known to negatively affect the application of Fe-based materials for treating electroplating wastewater. In this study, ferrous/ferric hydroxide complex (FHC) was successfully synthesized to greatly improve the electron-transfer efficiency for Cr(VI) removal; meanwhile, the effect of the interlayer type on Cr(VI) removal and the possible removal mechanisms were well investigated. The sulfate interlayer of FHC was shown to strongly promote Cr(VI) removal, with 10.4 % and 29.0 % improvements over those achieved by chloride and carbonate interlayers, respectively, in close association with the amounts of surface structural Fe(II), surface –OH, and adsorbed water. The initial pH value was demonstrated to exert almost no effect on Cr(VI) removal by FHC(SO 4 2− ) owing to the buffering effect of the surface –OH. These results were different from some previously reported Cr(VI) removal of Fe-based materials. Moreover, the results of characterization and lab-scale experiments indicated that most of Cr(VI) binds rapidly with the surface –OH owing to electrostatic attraction. The peak at 618 cm −1 in the FT-IR spectra shifted to 549 cm −1 and new peaks at 576.79 and 586.39 eV in the XPS spectra were observed after the reaction, which was attributed to the formation of CrOOH and FeCr 2 O 4 owing to reduction and coprecipitation. Pilot-scale experiments revealed that the chemical cost and sludge yield during the FHC process decreased by 71.3 % and 54.1 %, respectively, compared with those in the NaHSO 3 process. These findings provide new insight for utilizing different interlayers to improve the reactivity of FHCs in remediating electroplating wastewater containing Cr(VI).
研究领域:
环境科学
·
化学工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Haoran Song,Rui Zhou,Shiting Pan,Yuwei Wang,Yang Cai,Qingyun Li,Ting Wu,Yongming Shen,Changping Li
Treatment of landfill leachate and recovery of recycled water in photothermal activation of peroxydisulfate (PDS) process using iron nanoparticles/MXene-coated polyurethane sponge module (Fe NPs/MXene@PU) were investigated from lab to pilot scale for the first time. In the module preparation process, nanoscale zero-valent iron (NZVI) and iron oxides were in situ generated on Ti 3 C 2 MXene surface. Fe NPs/MXene@PU module reached the 147.7 °C interfacial temperature due to the synergistic effect of MXene and in-situ generated Fe NPs. Sulfate radical (SO 4 •- ) and hydroxyl radical (HO • ) were generated in photothermal activation of PDS process. Under optimized reaction conditions, almost complete removal of chemical oxygen demand (COD), nitrate nitrogen (NO 3 – ), ammonia nitrogen (NH 3 -N) and total phosphorus (TP) of landfill leachate was achieved after about 40 d pilot-scale experiment in the open air. The recycled water collected by natural condensation was also purified, which contained ultralow concentrations of COD, NH 3 -N, NO 3 – , TP and heavy metals and met the discharge limits of various countries. This study provides an eco-friendly and energy-efficient technology for treatment of landfill leachate and recovery of recycled water.
研究领域:
环境科学
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材料科学
·
水处理工程
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Zhaoying Liu,Yuan Kong,Kan Yang,Guangyun Chen,Ming Li
Background The overuse of ampicillin (AMP), a pivotal antibiotic, poses severe environmental and ecological risks, necessitating portable and precise monitoring. Results A highly integrated Lab-in-Tube biosensing platform based on Ag + -coupled hairpin (Ag@hairpin) aptamer and gold nanoparticles-immobilized DNA (AuNP@DNA) hydrogel for the portable and ultrasensitive detection of AMP was developed. In this pre-assembled tube platform, Ag@hairpin aptamers and AuNP@DNA hydrogels were immobilized on the inner bottom and cap of the tube, respectively. Upon introduction of AMP, the released Ag + triggered Ag-DNAzyme to cleave S-DNA, generating abundant AuNP. These AuNP were subsequently introduced into a glucose/FeNi-doped polydopamine nanozyme (FeNi@PDA) system to generate ultrasensitive colorimetric signals. The biosensing requires only a simple two-step upside down operation in the sealed tube, achieving an excellent limit of detection (LOD) of 1.02 pg/mL. The platform demonstrated high specificity (cross-reactivity less than 4.31%) and practical applicability in environmental samples. Furthermore, the advantages of miniaturized platform, simplified operation and portability had demonstrated. Significance By integrating accurate biosensing with an ultrasensitive enzyme cascade system, this Lab-in-Tube platform may offer an advanced strategy for the portable and on-site detection of other targets.
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mushi Li,Xi Wang,Mingyu Ma,Juan Wu,Yang Chen,Jinbao Ou,Xinyue Chen,Qianying Zhou,Wenjing Sang,Shihong Xu,Li Cai,Dengxin Li
This study focused on the treatment of high-concentration pharmaceutical wastewater with high-salinity by pretreatment of chemical synthesis coupling with evaporation. Via chemical synthesis, this pretreatment process was developed to remove the organic matter that inhibited evaporation of wastewater, resulting in improved evaporation efficiency. Lab-scale results demonstrated that under the optimum conditions (∼pH 9, 2 mL/L demulsifier, 98 mM/L H 2 O 2 , 3 h of aeration time, 80 °C, 500 mg/L PAC, and 20 mg/L PAM), 54.9 % of COD was successfully removed by single pretreatment and 98.6 % after coupling with evaporation. Compared to the 80.1 % COD removal efficiency of direct evaporation of 100 mL with 30 min, the pretreatment process coupling with evaporation demonstrated superior performance and halved the evaporation time. A pilot-scale chemical synthesis pretreatment (120 t/d) was further developed to treat pharmaceutical wastewater of both high COD concentrations and high salinity. Under the optimal conditions determined in the lab-scale experiments, the pilot-scale achieved a COD removal efficiency of 49 % with a single pretreatment and 95.8 % after coupling with mechanical vapor recompression (MVR). Additionally, the obtained salt appeared visually clean and white compared to that obtained from the treated wastewater without pretreatment. Furthermore, to elucidate the possible mechanisms driving the efficient removal of COD, GC–MS was performed on the wastewater before and after pretreatment, and the resulting products from pretreatment in pilot-scale applications were further determined by both FTIR and GC–MS. It was proposed that the aldol condensation reaction increased the formation of carbon chains to macromolecular compounds might be the possible mechanism. This study gives insights into the future application of low-investment and low-cost technology in the treatment of high-concentration pharmaceutical wastewater with high salinity in industry.
研究领域:
环境工程
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化工技术
·
废水处理
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DOI
高影响因子
TALANTA
·
2011
·
IF 3.722
Yang Wang,Jie Tang,Xiaoyu Luo,Xiaoya Hu,Chun Yang,Qin Xu
In this work, a kojic acid electrochemical sensor, based on a non-covalent molecularly imprinted polymer (MIP) modified electrode, had been fabricated in the lab-on-valve system. The sensitive layer was synthesized by cyclic voltammetry using o -phenylenediamine as the functional monomer and kojic acid as the template. The template molecules were then removed from the modified electrode surface by washing with NaOH solution. Differential pulse voltammetry method using ferricyanide as probe was applied as the analytical technique, after extraction of kojic acid on the electrode. Chemical and flow parameters associated with the extraction process were investigated. The response recorded with the imprinted sensor exhibited a response in a range of 0.01–0.2 μmol L −1 with a detection limit of 3 nmol L −1 . The interference studies showed that the MIP modified electrode had excellent selectivity. Furthermore, the proposed MIP electrode exhibited good sensitivity and low sample/reagent consumption, and the sensor could be applied to the determination kojic acid in cosmetics samples.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
DOI
高影响因子
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