Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 7
·1454-1460
·
IF 4.543
Zeying Zhang,Jin Xie,Jie Yu,Zhisong Lu,Yingshuai Liu
Although bioenzymes or artificial nanoenzymes-catalyzed reactions can efficiently amplify the detection signal in immunosorbent assays, the cascade reactions are sensitive to various conditions, including pH, temperature, time, and so on, resulting in poor reproducibility and dose-dependent response. In this work, a novel colorimetric immunoassay strategy was developed using an iron oxide magnetic nanoparticle (MNP) as a label for signal generation and amplification without the need for subsequent cascade reactions. The signal intensity is directly proportional to the level of targeting analytes, providing a more accurate quantification and a better reproducibility. The measurable color change results from the formation of a soluble red complex between bathophenanthrolinedisulfonic acid disodium salt (BPT) and Fe2+ released from dissolution of iron oxide MNP. The MNP, acting as an iron pool, provides a large amount of Fe2+ after its dissolution, thus promoting an efficient signal amplification. A qualitative evaluation is directly observed with the naked eye, while quantitative detection is achieved by measuring the absorbance of the colored solution using a UV-Vis spectrophotometer. Under optimal conditions, a limit of detection (LOD) at 3.6 pg mL−1 was achieved with a cancer biomarker carcinoembryonic antigen (CEA) as a model analyte, demonstrating its great potential for early cancer diagnosis. Moreover, the developed colorimetric assay method can be easily adapted to detection of other biomolecules (nucleic acids, saccharides, small molecules and so on) by simply changing the recognition pairs.
研究领域:
分析化学
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纳米技术
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生物医学工程
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癌症诊断
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DOI
PubMed
高影响因子
Nano Today
·
2024
·
IF 13.2
Qizhi Liang,Qianxi Zhou,Hai Shi,Shuai Wu,Jiahua Yang,Tianyu Zeng,Jing Zhao,Yongmei Yin,Genxi Li
The controllable assembly of metal-organic frameworks (MOFs) nanomaterials is an effective means to enhance their application capabilities. In this paper, a novel method is reported to control MOFs assembly by using a DNA framework material, a tetrahedral DNA nanostructure (TDN) labeled with phosphate groups at the vertex. In particular, various well-defined superstructures can be tailored by regulating the number of phosphate groups that coordinate with catalytic Zr-MOFs to create polymeric MOFs superstructures (Poly(MOFs)), exhibiting excellent catalytic activities and molecular recognition ability as well as greatly amplified signal readout. Furthermore, based on the DNA framework-controlled Poly(MOFs), a colorimetric biosensor with excellent analytical performance is fabricated for improving the diagnosis of human epidermal growth factor receptor 2 (HER2)-positive breast cancer. It is demonstrated that the quantification of HER2 protein can be achieved at molecular level with the limit of detection (LOD) of 12 pg L -1 and cellular level with the LOD of 10 cells. More importantly, the detection of HER2 in the serum of breast cancer patients have been achieved, thus HER2-positive and triple-negative breast cancer patients can be well distinguished. Considering the substitutability of the target, this study shows great potential in biomedical research as well as clinical applications.
研究领域:
材料科学
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纳米技术
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生物医学工程
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癌症诊断
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xiaojuan Yi,Yonghua Yuan,Min Qing,Lulu Wang,Huizhen Li,Lijuan Bai
Glucose management is an important part of disease control for diabetes patients, thus the development of a rapid and real-time point of care testing (POCT) device for monitoring blood glucose is of great significance. In this work, a paper-based analytical device (PAD) is constructed by combining acetylene black (AB)-hemin complex modified filter paper as sensing platform with a smartphone as signal detector. Large specific surface area of AB decreases the self-associate and aggregate of hemin in aqueous solution, resulting in improved peroxidase-like activity of hemin. Compared with graphene oxide supported hemin, AB-hemin exhibits superior signal response on paper. Glucose oxidase (GOx) catalyzes the conversion of blood glucose to hydrogen peroxide, and then AB-hemin complex catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMB oxidized products (TMB + ) in the presence of hydrogen peroxide, thus achieving the visual detection of blood glucose. In optimal conditions, PAD provides an applicable linear range from 0.2 mM to 30 mM and a low limit of detection (LOD) (0.06 mM). Notably, the detection accuracy of the developed paper-based sensor is in good agreement with that of the commercially available blood glucose meter (p > 0.05). Moreover, the proposed PAD presents high recoveries from 95.4% to 112% (RSD ≤ 3.2%), and therefore holds great potential for glucose monitoring and diabetes diagnosis.
研究领域:
生物医学工程
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分析化学
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即时检测
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糖尿病管理
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Enming Miao,Nazhen Zhang,Shiwei Lu,Yufeng Hu,Lin Fu,Hao Zhou,Jingjing Zhan,Minghuo Wu
A simple magnetization method was developed for the preparation of magnetic materials from conventional solid phase packing though coprecipitation and solvothermal approaches. And the prepared magnetic materials were used for magnetic solid phase extraction (MSPE) of biogenic amines (BAs) from beers. Furthermore, to improve the analytical throughput, a solid phase “on-situ” quadraplex isotope dimethyl labeling method was developed for the quantification of BAs by liquid chromatography-high resolution mass spectrometry (LC HRMS). Compared to conventional in-solution labeling, the “on-situ” labeling could simplify the sample preparation procedure and efficiently remove the residuals such as inorganic salts and excessive labeling reagents. The quadraplex labeling, which enabled three real samples and one internal standard sample to be analyzed simultaneously in a single LC HRMS run. For the tested 8 BAs (cadaverine, phenethylamine, spermine, spermidine, tyramine, histamine, putrescine and tryptamine), LODs of 0.02–0.05 μg/L and LOQs of 0.05–0.1 μg/L were achieved at good reproducibility (RSD of 0.5–4.6% and 2.2–7.0% for intra- and inter-day reproducibility, respectively). With this method, six beer samples were analyzed, and these 8 BAs were all detected in the range of low μg/L to 2.9 mg/L, which were lower than maximal residual level (MRL) required in the regulations of China and EU.
研究领域:
分析化学
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食品科学
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质谱分析
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Yujiao Hou,Nan Long,Boyu Jia,Xiaofang Liao,Minghan Yang,Lizhu Fu,Lidong Zhou,Ping Sheng,Weijun Kong
The prevalent ochratoxin A (OTA) contamination in food has increased the urgency to develop sensitive and accurate detection methods. This study reported a conveniently fabricated label-free electrochemical (EC) aptasensor for ultrasensitive detection of OTA based on the competitive binding of OTA with its specific aptamer without any signal amplification strategy. A gold electrode (AuE) was modified with a compact layer of double-stranded DNA (dsDNA), obtained via OTA aptamer and complementary DNA (cDNA) hybridization, to act as a barrier for redox probe [Fe(CN) 6 ] 3-/4- to AuE access. This led to a high initial electron-transfer resistance (R ct ), recorded via electrochemical impedance spectroscopy (EIS). Since the aptamer displayed a higher affinity to OTA than cDNA, the added OTA triggered aptamer dissociation from AuE to form the OTA-aptamer complex, obviously decreasing the R ct . The target-induced variation in the resistance response strongly depended on the OTA concentration. Optimum conditions improved the linear relationship between R ct and the logarithm of OTA concentration in a range of 0.05–10 ng/mL with a limit of detection (LOD) of 0.05 ng/mL for OTA. Recoveries of 74.8–105% with relative standard deviations (RSDs) of 8.86–13.09% were obtained in the spiked malt samples. The Real-world detection of OTA in the actual malt samples confirmed the reliability of the convenient, cost-effective, and highly specific impedimetric aptasensor, exhibiting significant promise for extended application in food control to identify more hazardous contaminants by altering the corresponding aptamers.
研究领域:
食品科学
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分析化学
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电化学
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毒理学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yuandong Lin,Nian Lu,Ji Ma,Jun-Hu Cheng,Da-Wen Sun
An emerging fluorescent ratiometric aptasensor based on gold nanoclusters (AuNCs) with aggregation-induced emission (AIE) properties was prepared and studied for deoxynivalenol (DON) detection. The ratiometric aptasensor used red fluorescent AuNCs 620 labelled with DON aptamer (Apt-AuNCs 620 ) as an indicator and green fluorescent AuNCs 519 modified by complementary DNA (cDNA) and magnetic beads (MBs) as internal reference, namely MBs-cDNA-AuNCs 519 . Under the optimal conditions, the aptasensor exhibited two good linear ranges of 0.1–50 and 50–5000 pg/mL for DON detection with coefficient of determination (R 2 ) of 0.9937 and 0.9928, respectively, and the low detection limit (LOD) of 4.09 pg/mL was achieved. Furthermore, this aptasensor was feasible to detect DON in positive wheat samples, and the results were in line with those from HPLC and ELISA, thus providing a promising route to detect DON with high sensitivity in cereals, even for other mycotoxins by replacing the suitable aptamer and cDNA.
研究领域:
分析化学
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纳米技术
·
食品安全
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Xiangqian Li,Jiapei Gu,Zhan Zhou,Wanqiang Liu,Jinwei Gao,Qianming Wang
The versatility of the organic fluorophores enables us to track its emission curves and sharp luminescence changes under controllable conditions. A tetraphenylethylene-based fluorescent probe (1-methyl-4-(4-(1,2,2-triphenylvinyl)styryl)pyridin-1-ium iodide, abbreviated as TPE-py-I ) has been synthesized and yields efficient red emission in organic phase (DMSO). The incorporation of ClO − in its DMSO solution triggers efficient “on-off” quenching effect due to ions exchange from iodide to hypochlorite. The inactivation of the original red luminescence can be also realized by the addition of water and the typical aggregation-caused-quenching (ACQ) has been observed. Unexpectedly, such ACQ process will be suppressed and lead to an aggregation-induced-emission (AIE) effect, providing distinct fluorescent read-out from pale-red to bright yellow in the presence of hypochlorite. The limit of detection (LOD) has been calculated to be 0.25 μM via ratiometric operation with an excellent linear range from 1 μM to 50 μM. This non-invasive and in-situ detection of ClO − via AIE modes can be employed as an indicator for the evaluation of ions responses in real environments.
研究领域:
材料科学
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分析化学
·
光学传感
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产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 46
·26029-26035
·
IF 2.936
Ming Li,Yuanyuan Zhang,Rujin Zhao,Zhenjiang Liu,Xia Hong,Yin Cui,Yonglai Xue,Daolin Du
The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10), half-maximal inhibition concentration (IC50) and detection range (IC20–IC80) of BSA-IMB are 0.00579, 0.573 and 0.0183–17.9 ng mL−1, respectively, for AFB1. The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.
研究领域:
食品安全
·
分析化学
·
毒理学
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农业科学
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产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 82
·期 8
·1285-1293
·
IF 1.401
Wang Huimin,Guo Bin,Pang Xiaoya,Yu Huan,Liu Haiyan,Yan Hongyuan,Han Dandan,Guo Huaizhong,Bai Ligai
A poly(NMA-ST- co -TAIC-EDMA) monolithic column was prepared by redox initiation method using N -methylolacrylamide (NMA) and styrene (ST) as co-monomers. The composite monolith was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm and was used as a solid phase extraction (SPE) absorbent for enrichment of β -sitosterol by high performance liquid chromatography (HPLC). Under the optimum conditions for extraction and determination, the calibration equation was y = 1.02247 x + 0.1766; the linear range was 0.015–0.75 mg mL −1 and the linear regression coefficient was 0.998. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.006 mg mL −1 and 0.02 mg mL −1 , respectively. The spiked recoveries of β -sitosterol in edible oil samples were 83.60–103.88%; precisions for intra-day and inter-day assays presented as relative standard deviations were less than 6.0% and 6.2%, respectively. The enrichment factor of β -sitosterol was 60 and the results showed that the monolithic column had high selectivity and good permeability as an on-line SPE absorbent for the enrichment and determination of β -sitosterol from edible oils.
研究领域:
分析化学
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食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Kangkang Wang,Meng Wang,Yuli Kou,Changyan Guo,Xin Ma,Bingren Tian,Ying Qi,Wei Wang,Jide Wang
A rapid and sensitive analytical method has been proposed to monitor simultaneously four nitrofuran metabolites (NFMs) in meat products by ultra-high performance liquid chromatography–fluorescence detection (UHPLC-FLD). These NFMs were derivatized by 4-( N,N diphenylamino)benzaldehyde (DABA) by thermostatic ultrasonication within 20 min. The results confirmed that the four NFMs derivatives had excellent fluorescence response at specific excitation (λ ex = 260 nm) and emission (λ em = 500 nm) wavelengths, and all NFMs derivatives could be separated entirely on the reversed-phase Syncronis C18 column within 10 min. The limits of detection (LOD) and quantitation (LOQ) in this new method were 0.21 to 0.28 and 0.69 to 0.92 μg/kg, respectively. The four NFMs displayed excellent linearity (R > 0.998), and the recovery rate was satisfactory, ranging from 91.6% to 104.0%. This method successfully determined the NFMs in fish, shrimp and chicken. The method is highly sensitive and accurate, therefore, it may have potential for detecting four NFMs residues in other foodstuffs.
研究领域:
分析化学
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食品科学
·
药物分析
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-10
·
IF 6.408
Zhou Xuemei,Qi Yuji,Tang Yulian,Gao Hui,Lv Li,Lei Xingyu,Hu Lei,Yan Zhengquan
To enhance the peroxidase-like performance and its application in detection of toxic o -aminophenol ( o -AP), a kind of bimetal Cu–Zn oxide-based mesoporous nanosphere (Cu 2/3 Zn 1/3 O PNPs) was constructed under microwave-radiation conditions. Its mesoporous microstructure and peroxidase-like catalytic activity were investigated in detail. The results showed that Cu 2/3 Zn 1/3 O PNPs possessed a high specific surface area of 34.89 m 2 g −1 and a well-distributed mesoporous size of approximate 6.07 nm, which endowed the superior peroxidase-like performance. The material catalyzes the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) with K m / V max of 0.104 mM/3.79 × 10 −8 M·s −1 in the presence of H 2 O 2 . Especially o -AP could exclusively deteriorate the characteristic UV–Vis absorbance intensity at 653 nm ( A 653 ) of the Cu 2/3 Zn 1/3 O PNPs-TMB-H 2 O 2 system with obvious color change from blue to colorless. Under the optimal conditions, the effect of some interfering substances was low and the limit of detection (LOD) for o -AP was 1.65 × 10 −8 mol/L (S/N = 3). When applied to the colorimetric detection of o -AP in practice, the recovery was between 96.1 and 107.2% with R.S.D. less than 2.04%. The mechanism of synergic-enhancement peroxidase-mimic activity of Cu 2/3 Zn 1/3 O PNPs and its exclusive colorimetric response to o -AP were proposed as well. Graphical abstract
研究领域:
纳米技术
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yunlong Nie,Fang Zhou,Chenye Wang
A simple paper-based microfluidic device was fabricated to simultaneously detect multiple targets. Microfluidic paper-based analytical devices (μPAD) comprise a single-layer moving sliding PAD (SPAD) to control the flow channel switch together with a folding origami PAD (OPAD) to test the target analytes. The facile assembly without any splicing materials avoids cross-contamination and non-specific adsorption of joining materials that may be caused by multi-target detection. The concentration of Fe(III), Ni(II), Cr(VI), and nitrite in standard solutions and actual aqueous solutions was successfully determined using the designed μPAD. The μPAD was able to achieve LOD of 3.3 mg/L, 1.3 mg/L, 0.35 mg/L, 0.28 mg/L for Fe (III), Ni (II), Cr (VI), and nitrite, respectively. The designed SOPAD exhibits improved stability, with a deviation of less than 7% compared to conventional analytical methods (ICP-OES and UV). Our work demonstrates that this 3D PAD holds great promise and a wide scope in environmental monitoring, biochemical analysis, food testing and other testing industries.
研究领域:
微流控技术
·
分析化学
·
环境科学
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生物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）