Food Chemistry-X
·
2023
·
IF 6.1
Gengan Du,Qi Guo,Liyue Qiang,Shuaidan Chang,Xiaohai Yan,Hong Chen,Yahong Yuan,Tianli Yue
This study aimed to evaluate the application of encapsulated L . plantarum and eugenol as potential biocontrol agents in sliced apples. The combined encapsulated L . plantarum and eugenol treatment was more effective than separate encapsulated L . plantarum and eugenol treatments, with regards to browning inhibition and consumers panel test. The application of encapsulated L . plantarum and eugenol reduced the decline of the physicochemical qualities of the samples, and improved the ability of antioxidant enzymes to scavenge reactive oxygen species. Furthermore, reductions in the growth of L . plantarum of only 1.72 log CFU/g were observed after 15 days of storage at 4 °C for samples treated with encapsulated L . plantarum and eugenol. Results suggest the combined encapsulated L . plantarum and eugenol appears to be a promising method to protect fresh-cut apples from food-borne pathogens while maintaining the visual appearance.
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高影响因子
Food & Function
·
2020
·Vol. 11
·期 4
·3156-3166
·
IF 4.171
Na Zhang,Chen Li,Zhihua Niu,Hongyan Kang,Miaoshu Wang,Bo Zhang,Hongtao Tian
Immunosuppression is a manifestation imbalance in the immune system, often during unhealthy states. In recent years, lactic acid bacteria (LAB) have been found to be important components of the body's innate immune system, and indispensable to maintaining normal immune function. Lactobacillus plantarum BF_15, a novel strain isolated from the feces of breast-fed infants, which has shown potential as an immunomodulator in vitro. In the present study, with the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) based on RNA-polymerase beta subunit encoding gene (rpoB) to analyze the colonization of L. plantarum BF_15 in the intestine of mice. In addition, Lactobacillus rhamnosus GG (LGG) as a positive control strain, by measuring immune-related indexes and the diversity of intestinal microbiota, the effects of BF_15 on immunoregulation and intestinal microbiota dysbiosis were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of intestinal microbiota. Results did indeed support this notion that BF_15 did colonize murine intestines well, in which it could still be detected in mice feces 14 days after stopping the probiotic administration. Moreover, BF_15 found to protect mice against reduction in the levels of several immune-related indicators, including the thymus and spleen indexes, splenic lymphocyte proliferation, toe swelling degree, serum hemolysin-antibody level, and macrophage phagocytosis index, triggered by high-dose (200 mg kg−1) intraperitoneal administration of cyclophosphamide (CTX). In addition, the strain was also found to effectively balance intestinal microbiota dysbiosis in the mice. Collectively, these results indicated that L. plantarum BF_15 can not only successfully colonize murine intestines, but also can effectively alleviate CTX-induced immunosuppression, once established, by rebalancing the intestinal microbiota. This, therefore, provides strong evidence for the view that BF_15 has the potential to become a highly effective immunomodulating probiotic in human microbiota as well.
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高影响因子
Food & Function
·
2023
·
IF 6.1
Yanyan Chen,Fei Fei,Lingling Ding,Shiyuan Wen,Caifang Ren,Aihua Gong
The microecological stability of the gut microbiota plays a pivotal role in both preventing and treating colorectal cancer (CRC). This study investigated whether Lactobacillus plantarum CBT (LP-CBT) prevents CRC by inducing alterations in the gut microbiota composition and associated metabolites. The results showed that the LP-CBT inhibited colorectal tumorigenesis in azoxymethane/dextran sulfate sodium (AOM/DSS) treated mice by repairing intestinal barrier function. Furthermore, LP-CBT decreased pro-inflammatory cytokines and anti-inflammatory cytokines. Importantly, LP-CBT remodeled intestinal homeostasis by increasing probiotics (Coprococcus, Mucispirillum, and Lactobacillus) and reducing harmful bacteria (Dorea, Shigella, Alistipes, Paraprevotella, Bacteroides, Sutterella, Turicibacter, Bifidobacterium, Clostridium, and Allobaculum), significantly influencing arginine biosynthesis. Therefore, LP-CBT-treatment regulated invertase and metabolites associated with the arginine pathway (carbamoyl phosphate, carboxymethyl proline, L-lysine, 10,11 -Epoxy- 3-geranylgeranylindole, n-(6)- [(Indol-3-yl) acetyl]-L-lysine, citrulline, N2 -succinyl-L-ornithine, and (5 -L-Glutamyl)-L-glutamate). Furthermore, the inhibitory effect of LP-CBT on colorectal cancer was further confirmed by the MC38 subcutaneous tumor model. Collectively, these findings offer compelling evidence to support the potential of LP-CBT as a viable preventive strategy against CRC.
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Cell Metabolism
·
2023
·
IF 29
Qingqing Zhang,Qing Zhao,Tao Li,Longya Lu,Fei Wang,Hong Zhang,Zhi Liu,Huihui Ma,Qihui Zhu,Jingjing Wang,Xuemei Zhang,Yang Pei,Qisha Liu,Yuyu Xu,Jinlong Qie,Xiaoting Luan,Zhibin Hu,Xingyin Liu
Previous studies have shown that Lactobacillus species play a role in ameliorating colorectal cancer (CRC) in a mouse model. However, the underlying mechanisms remain largely unknown. Here, we found that administration of a probiotic strain, Lactobacillus plantarum L168 and its metabolite, indole-3-lactic acid, ameliorated intestinal inflammation, tumor growth, and gut dysbiosis. Mechanistically, we indicated that indole-3-lactic acid accelerated IL12a production in dendritic cells by enhancing H3K27ac binding at the enhancer regions of IL12a that contributed to priming CD8+ T cell immunity against tumor growth. Furthermore, indole-3-lactic acid was found to transcriptionally inhibit Saa3 expression related to cholesterol metabolism of CD8+ T cells through changing chromatin accessibility and subsequent enhancing function of tumor-infiltrating CD8+ T cells. Together, our findings provide new insights into the epigenetic regulation of probiotics-mediated anti-tumor immunity and suggest the potential of L. plantarum L168 and indole-3-lactic acid to develop therapeutic strategies for patients with CRC.
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Wei Li,Liming Liu,Huafeng Tian,Xiaogang Luo,Shilin Liu
Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function. Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process. The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region. The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 10 6 cfu/mL at 210 min. In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 10 6 cfu/mL after the storage of 160 days at 4 °C. The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
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高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Hujun Xie,Xin Luo,Ying Gao,Min Huang,Gerui Ren,Rongmi Zhou,Yulu Sun,Haoxin Ye,Qunfang Lei,Wenjun Fang,Yong-Quan Xu
Probiotics and epigallocatechin gallate (EGCG) are sensitive to external conditions. In order to improve the survival rate of Lactobacillus plantarum (LP) and the activity of EGCG during storage, freeze-drying and gastrointestinal digestion, the LP and EGCG are co-encapsulated for the first time by extrusion using alginate-whey protein isolate (ALG-WPI) and alginate-pectin-whey protein isolate (ALG-PEC-WPI) biocomposites as wall materials, which can protect the encapsulated LP and EGCG during the simulated gastric digestion and co-release LP and EGCG in the intestine. Compared with ALG-PEC-WPI-LP beads, the release rate of LP in ALG-PEC-WPI-LP-EGCG beads is significantly increased to 92.8%. Both co-encapsulated beads are formed through hydrogen bonding interaction, and EGCG exists in amorphous form. Two co-encapsulated beads ALG-WPI-LP-EGCG and ALG-PEC-WPI-LP-EGCG exhibit better survival rate for LP during freeze-drying and in vitro digestion. Experiments conducted in high-fat diet-fed rats indicate that serum total cholesterols and low-density lipoprotein cholesterols in co-encapsulated group are significantly lower than those of un-encapsulated group. The co-encapsulated LP and EGCG alleviate the gut dysbiosis and decrease the hepatic steatosis in high-fat diet-fed rats. Thus the ALG-PEC-WPI composite is a good carrier for protection and delivery of LP and EGCG.
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高影响因子
JOURNAL OF ANIMAL SCIENCE
·
2017
·Vol. 95
·期 2
·837-846
·
IF 1.863
Yang Xin,Li Long,Duan Yongle,Yang Xiaojun
The aim of this experiment was to study the antioxidant capacity of Lactobacillus plantarum JM113 isolated from healthy intestinal contents of Tibetan chicken and its protective effect on broiler chickens challenged with deoxynivalenol (DON). Compared with L. plantarum PZ01 and Enterococcus fecalis M23, L. plantarum JM113 demonstrated maximum reducing (P < 0.05) activity and resistance in the presence of 1.2 mmol/L hydrogen peroxide, and great scavenging ability (P < 0.05) against hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl radicals in vitro. For each strain, the antioxidant activities of live bacterial strains were greater (P < 0.05) than of cell free extracts and dead bacterial strains. To examine the antioxidant capacity of L. plantarum JM113 in vivo, 192 1-d-old Arbor Acres chicks were randomly divided into 4 treatments groups consisting of 6 replicates with 8 birds per replicate. The dietary treatments were 1) control; 2) control diet supplemented with L. plantarum JM113 at 1 × 109 cfu/kg; 3) control diet contaminated with DON at 10 mg/kg; 4) control diet contaminated with DON at 10 mg/kg and supplemented with L. plantarum JM113 at 1 × 109 cfu/kg. Dietary supplementation with DON decreased (P < 0.05) superoxide dismutase activity in serum and increased (P < 0.05) malondialdehyde in the jejunal mucosa of broilers, compared to the control. However, supplementation with L. plantarum JM113 to both the DON-contaminated diet and the control diet, caused a significant reduction (P < 0.05) in malondialdehyde activity in the jejunal mucosa. A reduction (P < 0.05) in expression of nuclear factor erythroid 2-related factor 2 was observed in the jejunal mucosa of broilers fed dietary supplementation with DON, whereas the mRNA levels of Nrf2 and its corresponding downstream HO-1 gene increased (P < 0.05) with L. plantarum JM113 treatment. Addition of L. plantarum JM113 resulted in longer villi (P < 0.05), even in combination with DON compared to the DON group. L. plantarum JM113 treatment, especially in the DON plus L. plantarum JM113 group, up-regulated (P < 0.05) the expression of claudin-1 mRNA. In conclusion, the present study demonstrates that the L. plantarum JM113 strain has great antioxidant activity and supplementation in feed protected the integrity of the intestinal barrier in broilers challenged with DON, suggesting its use for alleviation of negative effects of DON in poultry.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yejun Zhong,Lisen Feng,Hailin Zheng,Hui Zhang,Junzhen Zhong
Four novel colorful C-phycocyanin-pectin (CPC-PE) based emulsion gel beads co-encapsulated with curcumin (Cur), β-carotene (β-Car), capsaicin (Cap) or procyanidins (PAs) were established. It was found that only co-encapsulation of procyanidins could improve the viable count of Lactobacillus plantarum, which was increased from 6.66 to 7.14 log/CFU during the digestion process and from 8.6 to 8.98 log/CFU during the storage process. Firstly, the four colorful emulsions were investigated. Compared with other emulsions, the droplets size of the CPC-PE-PAs emulsion was the lowest, which decreased from 6.5 to 2.5 μm. In addition, the apparent viscosity was the highest and the microstructure of that was the most homogenous. After gel beads formed, it was found that pectin gel beads have more holes and grooves inside the beads. However, the other gel beads are relatively denser and more homogeneous internally. The characteristic diffraction peaks of Cur, Cap, β-Car, and PAs were absent from the XRD spectra of the gel beads, indicating that the four small molecules were completely encapsulated and formed amorphous complexes with the carriers, which helped to stabilize the gel-bead system. These results confirm CPC-PE-procyanidin beads as a groundbreaking naturally colored probiotic delivery system.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Muhammad Nawaz,Hafiz Muhammad Rizwan,Zia Ud Din Momand,Irfan Ullah,Jiayi He,Qian Zhou,Mingfu Wang
Apple peel, a byproduct of apple processing or consumption, is often discarded, contributing to environmental pollution and a considerable loss of valuable bioresources. This study explored the health-promoting potential of apple peel through fermentation with Lactobacillus plantarum ATCC14917. Our previous study indicated that this strain could increase the in vitro antioxidant activity of apple juice. In this study, we examined the phytochemical changes of apple peel extract (APe) fermented by Lactobacillus plantarum ATCC14917, as well as evaluating its beneficial effects against advanced glycation end products (AGEs)-induced cellular responses. In particular, the primary phytochemicals were identified as chlorogenic acid, rutin, isoquercitrin, quercetin-3-xyloside, and quercitrin in APe. After fermentation by L. plantarum ATCC14917, APe exhibited changes in phytochemical content, as well as induced pronounced anti-inflammatory and antioxidant activities. Notably, fermentation increased the levels of quercitrin and quercetin-3-xyloside, while slightly decreased the levels of chlorogenic acid, rutin, and isoquercitrin. The highest antioxidant activities were observed in APe at a concentration of 2.0 mg/mL after 72 h of fermentation (F-APe). Furthermore, F-APe significantly reduced the ROS level and inflammatory responses in RAW 264.7 cells by modulating RAGE/MAPKs/NF-κB signaling pathway, demonstrating enhanced potentials compared to non-fermented APe (NF-APe). Collectively, our findings provide the first evidence that L. plantarum ATCC14917 fermentation specifically converts apple peel waste into bioactive compounds with enhanced beneficial effects, offering a novel strategy for the prevention and management of diseases linked to AGEs.
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Systems Microbiology and Biomanufacturing
·
2022
·
IF 0
Zhu Jiufeng,Bai Yajun,Fan Tai-Ping,Zheng Xiaohui,Cai Yujie
Ethyl ( R )-4-chloro-3-hydroxybutyrate (( R )-CHBE), as a chiral intermediate, is widely used in the synthesis of various chiral drugs. In this study, we screened two aldo–keto reductases (LP-AKRs) from the probiotic Lactobacillus plantarum DSM20174, both with a molecular weight of approximately 31 kDa. Both enzymes could reduce 4-chloroacetoacetic acid ethyl ester (COBE) to produce ( R )-CHBE with an enantioselectivity value of 99%. When determining the kinetic parameter, the K m , K cat , and V max of LP-AKR5 and LP-AKR9 were 9.5 mM, 7.6 U/mg, 3.96 s −1 and 8.7 mM, 8.59 U/mg, 4.47 s −1 , respectively. Both LP-AKR5 and LP-AKR9 had an optimal reaction pH of 6 and could maintain a high level of stability at pH 6, allowing them to perform well in an acidic environment. LP-AKR5 and LP-AKR9 had optimal reaction temperatures of 30 °C and 40 °C, respectively. Metal ions had minimal influence on LP-AKR5 and LP-AKR9 enzyme activities. This series of enzymatic properties showed that LP-AKR5 and LP-AKR9 mined from Lactobacillus plantarum DSM20174 could asymmetrically catalyze the synthesis of ( R )-CHBE under weakly acidic circumstances, which could maintain product stability and provide a good foundation for industrial production.
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Frontiers in Nutrition
·
2023
·
IF 5
Cao Feiwei,Ding Qinchao,Zhuge Hui,Lai Shanglei,Chang Kaixin,Le Chunyan,Yang Guorong,Valencak Teresa G.,Li Songtao,Ren Daxi
This present study was designed to explore the protective role of Lactobacillus plantarum ZJUIDS14 against Non-alcoholic Fatty Liver Disease (NAFLD) in a high-fat-diet (HFD)-induced C57BL/6 mice model. The probiotic (109 CFU/every other day) was administered by oral gavage for 12 weeks. We found that L. plantarum ZJUIDS14 intervention significantly alleviated HFD related hepatic steatosis, liver damage, insulin resistance, and increased hepatic expression of peroxisome proliferator activated receptor α (PPAR-α) while stimulating the activation of AMP-activated protein kinase (AMPK). Furthermore, L. plantarum ZJUIDS14 improved mitochondrial function as reflected by an increase in dynamin related protein 1 (DRP1) and a decrease of proteins associated with oxidative phosphorylation (OXPHOS) after the treatment. Additionally, mice from the L. plantarum ZJUIDS14 group had a restored intestinal flora and homeostasis involving Coprostanoligenes group, Ruminococcaceae UCG-014, Allobaculum, Ruminiclostridium 1, and Roseburia. Meanwhile, these five genera exhibited a significant (negative or positive) association with ileum inflammation mRNA levels and SCFA contents, by Spearman’s correlation analysis. In general, our data demonstrated that L. plantarum ZJUIDS14 mitigates hepatic steatosis and liver damage induced by HFD. Specifically, they strengthened the integrity of the intestinal barrier, regulated gut microbiota, and improved mitochondrial function. Our data provide an experimental basis for L. plantarum ZJUIDS14 as a promising candidate to prevent NAFLD.
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Food Bioscience
·
2023
·
IF 5.2
Mengjiao Ruan,Yunxiao Xie,Chaoyi Zhou,Yan Li,Bin Li,Yangyang Zhang,Shilin Liu
Probiotics are considered to have the potential to be beneficial to health when consumed in adequate amounts. Maintaining excellent probiotic activity during food storage and gastrointestinal transport is a key challenge. In this study, a stable water-in-water (W/W) Pickering emulsion consisting of hydroxypropyl methylcellulose (HPMC) and dextran (DEX) was constructed. Cellulose nanocrystals (CNCs) were used as the solid stabilizers of the emulsion. The obtained emulsions had excellent long-term stability with no phase separation within 60 days. In addition, the pH and ionic strength had little influence on the stability performance of the emulsions. The W/W Pickering emulsion system was applied to the probiotic encapsulation, and the emulsion was microencapsulated by spray drying technique. The microscopy images showed that Lactobacillus plantarum tended to be enriched in the dispersed phase of W/W Pickering emulsions. The viable cell content of obtained microcapsules was 2.22 × 10 10 CFU/g after spray drying. Furthermore, the viable cell content in emulsion microcapsules was 3.38 × 10 6 CFU/g after the simulated digestion process in artificial gastrointestinal fluid. The microcapsules containing Lactobacillus plantarum also showed excellent stability during the storage. The viable cell content of microcapsules was 1.76 × 10 9 CFU/g at 4 °C after 90 days of storage. This study could provide a potential method by using W/W Pickering emulsion as a system for encapsulating probiotics, which would provide protection for probiotics during spray drying and gastrointestinal digestion.
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