Food Chemistry-X
·
2025
·
IF 8.2
Yinglin Jian,Ningli Wang,Zhiming Han,Yunchun Li,Ting Huo,Yuan Gong,Duolong Di,Ling Ma,Dong Pei,Jianfei Liu
Drying plays a significant role in determining the quality and bioactivity of wolfberries. This study compared four drying methods—natural drying (ND), hot air drying (HAD), vacuum freeze-drying (FD), and vacuum microwave combined with vacuum pulsation drying (VMD)—focusing on their effects on the appearance and bioactive content of wolfberries, particularly carotenoids and polysaccharides. Wolfberries dried using FD and V MD appeared plumper, smoother, and more brightly colored. Notably, VMD-treated samples (LB-V) retained the highest total carotenoid content (6.42 mg/g), including zeaxanthin (3.41 μg/g), β -carotene (52.05 μg/g), and zeaxanthin dipalmitate (139.72 μg/g). Polysaccharides (LBPs- V ) extracted from LB- V displayed a lower molecular weight (3.51 × 10 4 Da), a higher arabinose content (51.66 %), and reduced aggregation. Moreover, LBPs-V significantly decreased ALT and AST levels (by up to 67.53 % and 60.84 %), alleviated oxidative damage by reducing ROS, MDA, and NO levels (up to 84.28 %), and increased GSH (up to 77.51 %), thereby providing protective effects against ethanol- and APAP-induced liver injury. These findings highlight the significant role of drying techniques in preserving the bioactivity of wolfberries and provide valuable insights for optimizing their processing in functional food applications.
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Microorganisms
·
2026
·Vol. 14
·期 4
·940
·
IF 4.7
Haoran Yan,Miaomiao Zhang,Chuchen Gui,Huiwen Huang,Wenhao Wu,Zhaokun Chen,Yuansheng Wu,Shaohao Chen,Hongcan Huang,Huixian Lin,Yan Guo,Baichuan Deng,Lingna Zhang
Kennel-housed dogs may experience chronic stress affecting cognition. This study compared the effects ofLycium barbarumpolysaccharides (LBP) and glycopeptide (LbGP) on cognitive function in laboratory-kenneled poodles. Eighteen dogs were assigned to CON, LBP, or LbGP groups for 42 days. Cognitive tests were performed and serum, saliva, and feces were collected for subsequent analysis. Both supplements improved cognitive performance (e.g., increased the correct rate in the cylinder test by approximately 40.0%, bothp< 0.001). LBP enriched beneficial bacteria (FaecalibacteriumandBacteroides,p< 0.05), reduced pathogens (RomboutsiaandTerrisporobacter,p< 0.05), and predominantly influenced the indole pathway of tryptophan metabolism. LbGP specifically decreasedEscherichia-ShigellaandCorynebacterium, increased fecal SCFAs, and mainly targeted the 5-HT pathway. Both treatments regulated immune function (i.e., elevated IL-6 and IL-10) and antioxidant capacity; LBP significantly increased serum superoxide dismutase (SOD) levels by 6.8% (p< 0.01) and BDNF levels by 13.5% (p< 0.05), while LbGP elevated (p< 0.01) glutathione peroxidase (GSH-Px) levels by 20.9% and reduced salivary cortisol levels by 14.2% (p< 0.01). Overall, LBP and LbGP support canine cognition through distinct microbiota-related mechanisms, likely via the microbiota–gut–brain axis, suggesting their potential as functional feed additives for cognitive health.
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Advanced Science
·
2026
·
IF 14.1
Yuping Sa,Hui Yuan,Jingui Ma,Zhigang Yang,Mei Wang,Weibiao Wang,Lingling Yang,Fen Ma,Weiman Zhang,Gidion Wilson Mening'oo,Guoning Chen,Xueqin Ma
Current Alzheimer's drugs exhibit limited effectiveness, highlighting the necessity for multi-target treatments. This study developed an innovative and efficient screening platform combining hydrogen peroxide test strip-based colorimetric sensing with affinity chromatography for rapid identification of acetylcholinesterase (AChE) inhibitors from complex herbal medicines. Applying this strategy, from Lycium barbarum leaves, we identified three potent inhibitors: chlorogenic acid, N-acetyl-N'-caffeoylputrescine (NANCP), and N-caffeoylputrescine (NCP), with IC 50 ranging from 55.7 to 143.2 µ m . Molecular analyses confirmed their stable binding to AChE. In a D-galactose and AlCl 3 -induced Alzheimer's disease (AD) mouse model, NCP treatment significantly rescued cognitive deficits in AD mice, with the spontaneous alternation rate in the Y-maze test improved by up to 50%. It markedly reduced cerebral Aβ levels (by 54%) and pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, alleviated oxidative stress, and attenuated hippocampal neuronal damage. Mechanistically, NCP modulated glycerophospholipid metabolism, reshaped gut microbiota, and targeted the proteasome-autophagy pathway, revealing a multi-faceted synergistic mechanism. The research offers a new screening tool for AChE inhibitors and highlights a promising natural multi-target candidate, NCP, for AD therapy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yonggang Zhao,Xia Wu,Huijuan Zhao,Hongting Cao,Xin Meng,Xiaomin Wu,Fengchen Bi,Guanghua Li
Exertional heat stroke (EHS) presents a significant public health risk, with recent studies highlighting the role of arachidonic acid 15-lipoxygenase-1 (ALOX15)-mediated ferroptosis in EHS-induced cardiac damage. Although Lycium barbarum polysaccharides (LBP) have shown potential in treating heart diseases, their effectiveness is limited by poor targeting and low bioavailability. Herein, we delicately engineered a myocardial-targeted lycium barbarum polysaccharide-loaded nanoparticle (PCM/LBP@MPDA) for EHS myocardial injury treatment. PCM/LBP@MPDA achieves precise myocardial targeting through the PCM (WLSEAGPVVTVRALRGTGSW) and subsequently inhibits ferroptosis by scavenging reactive oxygen species and chelating iron. Upon immediate cellular uptake of PCM/LBP@MPDA by cardiomyocytes, MPDA could can chelate with iron ions and elininate reactive oxygen species, while the released LBP was capable of supressing ferroptosis by downregulating ALOX15 and upregulating glutathione peroxidase 4, thereby contributing to an effective decay of the cardiomyocytes ferroptosis and management of the cardiac function in EHS mice via suppressing oxidative stress and ferroptosis. Overall, our findings pave a broad avenue for precise myocardial injury treatment, and point out a promising direction for clinical translation.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Chunxiao Tao,Fazhen Luo,Yaxin Wang,Xiaoya Gao,Yuqi Cao,Keke Wang,Yan Xie
Baicalin (Ba) is a promising anti-inflammatory agent for treating ulcerative colitis (UC), but its efficacy is constrained by poor solubility and colon-targeting efficiency. Lycium barbarum polysaccharide (LBP), an anionic polysaccharide, not only restores gut microbiota dysbiosis but also exhibits excellent upper gastrointestinal stability and biocompatibility, making it a candidate for developing colon-targeted carriers. In this paper, the polymeric nanoparticles (Ba@MP/LBP NPs) were constructed using LBP, mannose, and Prunus persica kernel protein (PKP) for targeted delivery of baicalin to enhance its efficacy in UC treatment. The obtained Ba@MP/LBP NPs possessed core-shell spherical morphology, narrow particle-size distribution (∼230 nm), negative surface charge (−33.89 mV), and excellent baicalin encapsulation (94.26 %). With the aid of the LBP coating, Ba@MP/LBP NPs can tolerate upper gastrointestinal disturbances and transport Ba@MP NPs into colon, thereby facilitating baicalin uptake by inflammatory macrophages via mannose receptor-mediated endocytosis. Furthermore, the LBP coating strengthened Ba@MP NPs' ability to regulate the intestinal flora; hence, Ba@MP/LBP NPs can restore gut microbiota balance while suppressing pro-inflammatory factor secretion and oxidative stress, ultimately alleviating UC. More importantly, Ba@MP/LBP NPs exhibited favorable biosafety. Overall, this work is the first attempt to employ LBP and PKP as colon-targeting carriers, providing a promising nanomedicine for UC therapy.
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Food Bioscience
·
2022
·
IF 5.318
Yi-Long Ma,Yue Wang,Zheng-Fang Wu,Jie Mei,Wen-Qing Zhang,Ya-Fang Shang,Kiran Thakur,Zhao-Jun Wei
To assess the effect of the digestive system on the phenolics and antioxidant capacity of Lycium barbarum fruit, its extract was digested and analyzed in vitro . The results revealed that the phenolics/flavonoids contents and antioxidant capacity in the pH-treated group and digested group generally decreased by 4.39%–21.22% in the oral and intestinal digestions but increased by 1.26%–16.14% in the gastric digestion. The phenolic content and antioxidant capacity were highly correlated in digested groups ( r > 0.899, p < 0.01). Five main phenolics including rutin , p -coumaric acid and its hexoside derivatives were identified and quantified, and these phenolics displayed higher stability during the digestion, implying that they may be the potential functional phenolics of L. barbarum fruit.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Shuo Shi,Wentao Zhang,Xinnan Liu,Xinyi Ren,Min Li,Jing Sun,Yutang Wang,Tianli Yue,Jianlong Wang
Lycium barbarum L. polysaccharides (LBPs) with outstanding biological activities are of increasing interest. Traditional purification approaches are time-consuming and often involve toxic solvents that destroy the functionality and structure of polysaccharides. Herein, we report a sustainable and nondestructive strategy for purifying LBPs using graphene-based nano-decoloration. The amination of graphene oxide (GO) enables the resulted aminated reduced GO (NH 2 -rGO) with abundant sp 2 -hybridized carbon domains, displaying high adsorption capacity toward pigments in crude polysaccharides. As such, within 5 min, NH 2 -rGO can highly effectively and fast to decolor LBPs, with a high decoloration ratio of 98.72% and a high polysaccharides retention ratio of 95.62%. Importantly, compared with traditional decoloration methods, NH 2 -rGO is nondestructive toward LBPs and has good reusability. Moreover, it exhibited widespread-use decoloration performance to decolor several common plant species. Overall, our proposed nano-decoloration approach is a general-purpose, sustainable, and nondestructive method to purify LBPs.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Cunchun Dai,Quanying Li,Guiyu Zhou,Yunjie Liu,Xirui You,Binghu Fang
A novel nanoparticle (WSLM) encapsulating α-mangostin and Lycium barbarum polysaccharide for oral co-delivery. • WSLM effectively eradicates intracellular methicillin-resistant Staphylococcus aureus . • WSLM suppresses pro-inflammatory cytokines to alleviate the inflammatory response. • WSLM combats intestinal infection via integrated antibacterial and anti-inflammatory. Methicillin-resistant Staphylococcus aureus (MRSA) is a globally prevalent pathogen responsible for severe and often refractory gastrointestinal infections. Current therapeutic strategies predicated on bactericidal activity overlook the critical pathological consequence of bacterial lysis, which involves the release of cell components that trigger excessive host inflammatory responses and exacerbate intestinal injury. Inspired by the need for integrated host-pathogen modulation, we developed an oral co-delivery nanoparticle system (WSLM) based on chitosan and whey protein isolate, which encapsulates α-mangostin and Lycium barbarum polysaccharide, to enable coordinated antibacterial and anti-inflammatory intervention within the gastrointestinal tract. WSLM effectively inhibited MRSA biofilm formation and bacterial colonization while significantly attenuating infection-associated inflammation, reducing TNF-α and IL-6 levels by approximately 76.1% and 59.3%, respectively. In a mouse model of MRSA intestinal infection, oral administration of WSLM significantly alleviated intestinal pathology, restored colon length, and decreased bacterial load by approximately three orders of magnitude. Mechanistically, WSLM promoted epithelial barrier repair through the upregulation of tight junction proteins. Furthermore, microbiome analysis revealed that WSLM showed enrichment of beneficial commensal families, including Lachnospiraceae and Oscillospiraceae . Overall, this study has established a multifunctional nano-delivery system targeting bacterial infection and hyperinflammation simultaneously, providing an experimental paradigm for the treatment of bacterial intestinal infections. Download: Download high-res image (119KB) Download: Download full-size image
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Chun-Jie Bao,Meng-Qiu Liu,Ya-Nan Liu,Li-Qiang Zhao,Yu-Xuan Li,Xin-Lan Zhang,Fang Zhang,Sheng Guo,Er-Xin Shang,Jia-Lun Duan,Jin-Ao Duan
Current studies on plant-derived adjuvants focus on heterogeneous or partially characterized polysaccharides, while the adjuvanticity of structurally defined, arabinogalactan (AG)-rich polysaccharide in cancer nanovaccine settings remains unexplored. Here, we identify and characterize NLBPE1, a homogeneous AG-rich polysaccharide isolated from Lycium barbarum , which features an atypical arabinose-bridged structure. Unlike canonical AG-I or AG-II motifs, NLBPE1 contains galactose side chains connected to the rhamnogalacturonan backbone via → 5)- α -Ara f -(1 → linkages, forming a structurally distinct AG topology. Mechanistic studies demonstrate that NLBPE1 activates dendritic cells through the TLR4–IKK–NF-κB signaling axis, promoting antigen uptake, cross-presentation, and proinflammatory cytokine release. When co-formulated in a PLGA-based nanovaccine with a model antigen, NLBPE1 markedly enhances CD8 + T cell priming, leading to effective tumor control in vivo . Moreover, combination with anti-PD-1 immune checkpoint blockade yields synergistic antitumor responses in a melanoma model. Together, these findings establish NLBPE1 as a structurally and mechanistically distinct polysaccharide adjuvant with translational potential for next-generation cancer nanovaccine strategies.
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Food & Function
·
2020
·Vol. 11
·期 4
·3741-3748
·
IF 4.171
Wei Li,Mingbo Gao,Ting Han
Impairment of the intestinal barrier often occurs in inflammatory bowel diseases, and pro-inflammatory factors play a vital role in the pathogenesis of intestinal diseases. In our study, the potential protective effects of Lycium barbarum polysaccharides (LBP) against intestinal barrier dysfunction evoked by pro-inflammatory factors and its anti-inflammatory effects were investigated. Caco-2 cells were stimulated with or without tumor necrosis factor (TNF)-α in the presence or absence of LBP. Our findings showed that LBP assuaged the increase of paracellular permeability and the decrease of transepithelial electrical resistance (TER) in Caco-2 cells. In addition, LBP also prevented the secretion of pro-inflammatory markers (IL-8, IL-6, ICAM-1 and MCP-1) in TNF-α-challenged Caco-2 cells. Moreover, LBP inhibited the overexpression of tight junction (TJ) proteins (claudin-1, ZO-3, and occludin) and the increase of MLCK, pMLC, p-IκBα and NFκBp65 protein expression evoked by TNF-α was suppressed by LBP pre-incubation. This finding indicated that LBP improve TNF-α-evoked intestinal barrier dysfunction via suppressing the MLCK-MLC signaling pathway mediated by NFκB.
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JOURNAL OF FOOD QUALITY
·
2019
·
IF 1.36
Irma Belinda Yossa Nzeuwa,Baofu Guo,Ting Zhang,Liya Wang,Qian Ji,Hui Xia,Guiju Sun
Lycium fruits (Lycium barbarum, Lycium chinense) are mainly cultivated and distributed in Northwest China. The fruits and root bark have been used in Chinese traditional medicine for centuries. In this study, Lycium dry fruit extracts from the main cultivation areas in China together with a sample from Nepal were subjected to a comparative metabolic profiling, including total carbohydrate content, total phenolic content, vitamin C content, carotenoid content, and mineral contents. Results showed that there was a slight difference in contents of nutrients and phytochemicals among samples from different areas. The total carbohydrate content was higher in the sample from Guazhou, Gansu province (69.47%), with an average value of total carbohydrate content of 61.59%, while the highest total phenolic content value was 14.13 mgGAE/g from Nepal. Data concerning vitamin C content ranged between 33.15 and 113.8 mg/100 g, with an average value of 55.29 mg/100 g. Zeaxanthin dipalmitate content in Lycium dry fruits ranged from 419.34 to 1008.90 μg/g among the different samples, with the highest content (1008.90 μg/g) observed in Tianjing. It appeared that we could not clearly differentiate Lycium samples in terms of their metabolic and mineral profile. The quantitative difference observed among samples might be linked to soil composition and environmental aspect of the harvest place. Our results were somehow in the same range as those reported in the literature. Therefore, Lycium fruits could be used as a dietary source of natural function foods and be worthy of development and utilization.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 4
·1558
·
IF 4.9
Jiabin Huang,Haotian Wu,Ranran Gao,Lan Wu,Mengyue Wang,Yang Chu,Yuhua Shi,Li Xiang,Qinggang Yin
L-ascorbic acid (vitamin C, AA) is widely present in plants, but humans lack the ability to synthesize it independently. As a potent reducing agent, AA is susceptible to oxidation, making the enhancement of its stability crucial. 2-O-β-D-glucopyranosyl-L-ascorbic acid (AA-2βG) is a stable natural derivative of AA with glycosylation, initially discovered in the fruits ofLycium barbarum. Understanding the biosynthesis of AA-2βG is crucial for enhancing its production inL. barbarum. While the established biosynthesis pathway of AA constitutes the upstream of AA-2βG biosynthesis, the conclusive step of β-glycosylation remains unclear. We identified aL. barbarumcultivar by UPLC, ZN01, with a high content of AA-2βG, and compared its leaves, immature fruits, and mature fruits to a normal AA-2βG contentL. barbarumcultivar for metabolomic and transcriptomic analysis. The RNA-seq and RT-qPCR analysis revealed that the expression levels of genes involved in the AA biosynthesis pathway did not consistently correlate with AA-2βG content, suggesting that the final glycosylation step may be a key determinant of AA-2βG accumulation. Subsequently, utilizing phylogenetic and co-expression analysis, we identified ten UDP-glycosyltransferases (UGTs) and three β-glucosidases (BGLUs) which may be involved in the crucial step of the conversion from AA to AA-2βG, and the UGTs’ activities were predicted through molecular docking. Lastly, we speculated that the presence of the glycosylation process of AA might have a crucial role in maintaining AA homeostasis inL. barbarum, and deliberated on potential correlations between AA, carotenoids, and anthocyanins. Our integrated multi-omics analysis provides valuable insights into AA-2βG biosynthesis inL. barbarum, identifying thirteen candidate genes and highlighting the complex interplay between AA, carotenoids, and anthocyanins. These findings have implications for improving AA-2βG content inL. barbarum.
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危险品化学品经营许可证（带存储）