CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Xinhui Fan,Ke Li,Xuemei Qin,Zhenyu Li,Yuguang Du
Astragali Radix polysaccharides (APSs) exhibit a broad spectrum of biological activity, which is mainly related to immune regulation. At present, most available studies focus on total APSs or a certain component of APSs. However, structural structure study and screening for the anti-inflammatory activity of polysaccharides with different molecular weights (MW) have yet to be conducted. In this study, lipopolysaccharide (LPS)-induced RAW264.7 macrophages were used as a model to investigate the anti-inflammatory activity of APSs and its fractions. The results revealed that fraction APS-I had better anti-inflammatory effects than APS-II. After APS-Ⅰ was hydrolyzed by trifluoroacetic acid (TFA), the resulting degradation products oligosaccharides were fully methylated. These derivatized oligosaccharides were further analyzed by MALDI-TOF-MS and UPLC-Q-Exactive-MS/MS. The results showed that APS-Ⅰ was a hetero-polysaccharide with a molecular weight of about 2.0 × 106 Da, mainly consisting of glucose (46.8%) and galactose (34.4%). The degree of polymerization of Astragali Radix oligosaccharides (APOS) was 2-16. APOS were identified as 1,4-glucooligosaccharides and 1,4-galactooligosaccharides. The findings of this study lay the foundation for further elucidation of structure-function relationships of APSs and provide guidance for the development of anti-inflammatory drugs.
研究领域:
药理学
·
生物化学
·
免疫学
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DOI
PubMed
高影响因子
Chemical Science
·
2018
·Vol. 10
·期 1
·257-267
·
IF 9.063
Enhui Wu,Kun Feng,Rui Shi,Rui Lv,Fuzhong Ouyang,Shawn S. C. Li,Jun Zhong,Jian Liu
The soaring concerns globally on antibiotic overuse have made calls for the development of rapid and sensitive detection methods urgent. Here we report that the hybrid CuCoO–GO matrix allows for sensitive detection of various antibiotics in combination with MALDI TOF MS. The new matrix is composed of few-layered GO nanosheets decorated with CuCoO nanoparticles with an average size of 10 nm, and exhibits excellent aqueous suspensibility. Accurate quantitation of the sulfonamide antibiotics in milk samples have been demonstrated using a CuCoO–GO matrix and a stable isotope (C13)-labeled analyte as the internal standard. Our experiments have achieved lower limits of detection (LOD) by several hundred fold for the detection of a panel of representative antibiotics, in comparison with the literature reports. Both intrabacterial and extrabacterial residual antibiotics can be sensitively detected with our method. We have further investigated the molecular mechanism of the enhanced desorption/ionization efficiency by the CuCoO–GO matrix with synchrotron radiation techniques for the first time. This work provides a sensitive matrix enabling MALDI-TOF MS to be applied in small molecular analysis, but also presents a distinct perspective on the mechanism behind the material functions.
研究领域:
分析化学
·
纳米技术
·
材料科学
·
生物医学工程
·
质谱分析
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaopan Liu,Mengge Zhao,Lanlan Wang,Yuting Chen,Guanghui Niu,Xingjie Zhang,Lihong Liu,Changpo Chen
Carboxyl-functionalized covalent organic framework (COF HD ) was synthesized and successfully applied as both an adsorbent and MALDI-MS matrix for the ultrasensitive detection and quantitative analysis of illicit cationic dyes in food samples with low matrix effects. Benefitting from its great thermal stability and strong UV absorption properties, COF HD was an ideal matrix for cationic dyes with no matrix background interference, high ionization efficiency, excellent salt tolerance, and superior reproducibility. Furthermore, the merits of numerous adsorption sites, good water dispersibility, and electronegative natures facilitated the efficient and selective enrichment of cationic dyes from solutions. The proposed method was further applied for the quantitative analysis of CV and RB in real food samples, such as chili, red wine, beverage samples, exhibiting a wide linear range (0.2–40 ng mL −1 for CV and 0.8–200 ng mL −1 for RB with R 2 > 0.98), ultra-high sensitivity (at subppt levels), excellent precision and accurate, satisfactory recovery, and low matrix effects (91.7 %–96.8 %). This work establishes a robust, high-throughput analytical platform for the sensitive detection and precise quantification of illicit cationic dyes in complex food matrices, providing a reliable tool for food safety monitoring.
研究领域:
分析化学
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食品安全
·
材料科学
·
质谱分析
·
环境污染物检测
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DOI
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 4
·678
·
IF 4.1
Raquel Nunes Almeida da Silva,Karina Teixeira Magalhães-Guedes,Rogério Marcos de Oliveira Alves,Angélica Cristina Souza,Rosane Freitas Schwan,Marcelo Andrés Umsza-Guez
(1) Background: The identification of microorganisms includes traditional biochemical methods, molecular biology methods evaluating the conserved regions of rRNA, and the molecular biology of proteins (proteomics), such as MALDI-TOF MS mass spectrometry. This work aimed to identify the biodiversity of yeasts associated with stingless bee species’ honey and pollen,Melipona scutellaris,Nannotrigona testaceicornes, andTetragonisca angustula, from the region of São Gonçalo dos Campos-Bahia (BA) state, Brazil. (2) Methods: Cellular proteins were extracted from 2837 microbial isolates (pollen and honey) and identified via MALDI-TOF MS. The identified yeast species were also compared to the mass spectra of taxonomically well-characterized reference strains, available from the National Center of Biotechnology Information (NCBI) database. (3) Results: Nine yeast species were identified:Candida maltosa,Candida norvegica,Kazachstania telluris,Schizosaccharomyces pombe,Scheffersomyces insectosus,Meyerozyma guilliermondii,Brettanomyces bruxellensis,Kazachstania exigua, andStarmerella lactis-condensi. Nannotrigona testaceicornespollen had the highest number of yeast colonies. The yeastsBrettanomyces bruxellensisandKazachstania tellurisshowed high populations in the samples ofNannotrigona testaceicornesandMelipona scutellaris, respectively. This work shows that there is some sharing of the same species of yeast between honey and pollen from the same beehive. (4) Conclusions: A total of 71.84% of the identified species present a high level of confidence at the species level. Eight yeast species (Candida maltosa,Candida norvegica,Kazachstania telluris,Schizosaccharomyces pombe,Scheffersomyces insectosus,Meyerozyma guilliermondii,Kazachstania exigua, andStarmerella lactis-condensi) were found for the first time in the samples that the authors inspected. This contributes to the construction of new knowledge about the diversity of yeasts associated with stingless bee products, as well as to the possibility of the biotechnological application of some yeast species.
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 78
·63896-63902
·
IF 3.84
Qun Song,Wen-jie Zhao,He-xing Yin,Hong-zhen Lian
In this work, novel FeIII–tannic acid (TA) film-functionalized magnetic silica microspheres (designated Fe3O4@SiO2@FTA) were prepared via a facile deposition of one-step assembled FeIII–TA films on the surface of magnetic silica microspheres. The resulting Fe3O4@SiO2@FTA microspheres possessed strong magnetic responsiveness and good dispersity in aqueous solutions. We introduced low concentrations of standard peptide, standard protein, peptides in BSA tryptic digests and human urine to investigate the enrichment capability of the prepared nanocomposites. The enrichment process was very fast (only 5 min) and the peptide/protein–magnetic microsphere conjugates were readily separated by an external magnet. Moreover, the obtained conjugates can be directly analyzed by MALDI-TOF MS without any elution procedure. The enrichment performance was quite satisfying, indicating Fe3O4@SiO2@FTA microspheres as a universally applicable material for efficient and fast enrichment of peptides and proteins.
研究领域:
分析化学
·
蛋白质组学
·
材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Chen Ya-Shun,Xiao Hua-Ming,Wang Tian-Qi,Yu Lei,Feng Yu-Qi
A MALDI-TOF mass spectrometric method is described for the determination
of small molecule compounds with cis-diol. It is based on the use of a binary matrix
consisting of boron nitride (BN) and α-cyano-4-hydroxycinnamic acid that was
modified with the derivatization reagent of (3-(acridin-9-ylamino)phenyl)boronic
acid which can recognize cis-diols. The binary matrix is used for
desorption/ionization (DI) in the positive ion mode. The mechanism leading to DI
enhancement was investigated. The results imply that BN is beneficial for the DI
because it induces an enhancement in the positive ion mode. The boronic
acid-functionalized binary matrix was successfully applied to capture the glucose,
shikimic acid and quinic acid. The method was applied to the determination of
3-chloro-1,2-propanediol in plant oil.
Schematic representation of a method for detecting the
cis-diol compounds on matrix assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOF MS) using the binary
matrix of boron nitride (BN)/α-cyano-4-hydroxycinnamic acid (CHCA)
that was modified with (3-(acridin-9-ylamino)phenyl) boronic acid
(AYPBA).
研究领域:
分析化学
·
生物医学
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DOI
PubMed
高影响因子
Analytical Science Advances
·
2025
·Vol. 6
·期 2
·e70037
·
IF 4.1
Dan-ping Liu,Bi-cheng Yang,Qiao-yun Huang,Ping Liu,Hai-yan Luo,Mao-lin Yu,Jian-qiong Yang,An Jia,Yan-qiu Liu,Lin Xu,Bin Hu,Ting Huang
In this work, we developed a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method to directly analyze human whole blood samples. 1.0 µL of whole blood samples and 1.0 µL of optimized organic matrix were directly loaded onto the sample plates for MALDI-TOF-MS detection. A total of 59 whole blood samples were investigated in this work, including 23 healthy control samples, 28 α-globin gene triplication carriers with α-chain repetition, and eight deletional hemoglobin H disease (HbH) patients with α-chain deficiency. Human hemoglobin (Hb) chains with high signal-to-noise were directly observed. It is found that there is a change in signal ratios of α/β-chains of Hb from blood samples in thalassemia subtypes by comparing the ratios obtained from healthy blood samples. Analytical variability ( n = 6) of signal ratios of α/β-chains was found to be 3.33%–12.86% (intra-day), 4.02%–13.39% (inter-day), and 6.41%–15.32% (inter-laboratory), respectively. Furthermore, the results were validated using different MALDI-TOF MS approaches. Our results suggest that α/β-chains ratios of Hb could be an indicator for investigating thalassemia by MALDI-TOF-MS.
研究领域:
质谱分析
·
临床化学
·
血液学
·
遗传学
·
蛋白质组学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Hui-Wen Wang,Jia-Qi Wang,Yue-Qiu,Tong-Hao Cui,Jian-Cong Liao,Yi-Long Liu,Ya-Qin Liu,Yuan-Jiang Pan,Xian Li,Xiao-Yong Zhao
Universal 5-Da labeling creates internal standards for accurate N-glycome analysis. • Novel matrix 2-AIA/CHCA ensures uniform co-crystallization and high reproducibility. • H3N2F1X1 is a cross-species candidate biomarker for fruit cold stress. N-glycosylation is a crucial post-translational modification regulating protein function in plants. However, its accurate quantification by matrix-assisted laser desorption/ionization-mass spectrometry with time-of-flight is challenged by low glycoprotein abundance, poor glycan ionization, uneven matrix crystallization, and insufficient internal standards. Here, we introduce a novel 5-Da stable isotope labeling method, combining enzymatic 18 O-labeling (+2-Da) and reduction with NaBD 4 (+3-Da) on a portion of the sample itself to universally generate internal standards that eliminate isobaric interference. Furthermore, a binary matrix of 2-aminoisophthalic acid (2-AIA) plus α-cyano-4-hydroxycinnamic acid (CHCA) was developed, providing high sensitivity and homogeneous co-crystals. The integration of isotope labeling with 2-AIA/CHCA allows linear quantification ( R 2 > 0.99) over a two orders of magnitude. Applied to tomato and peach during cold storage, the method revealed that H3N2F1X1, the most abundant N-glycan in both fruits, was significantly downregulated, suggesting its role as a candidate biomarker for cold stress response and fruit quality control. Download: Download high-res image (204KB) Download: Download full-size image
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yanbo Hu,Yiming Li,Yuzhu Shen,Baochun Zhang,Jiaxin Liu,Yi Cao,Jun Zhao
In this study, six rare protopanaxadiol (PPD)-type ginsenosides, namely, F2, CK, C-Mc1, C-Mc, CO, and CY, were prepared using enzymatic hydrolysis. Firstly, major (PPD)-type ginsenosides, including Rb1, Rb2, Rc, and Rd, were biotransformed by employing single and combined β-glucosidases BaBgl1A and BaBgl3A. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses revealed that ginsenosides F2, CO, and C-Mc1 could be produced by BaBgl1A, while rare ginsenosides CK, CY, and C-Mc could be produced by the combined use of BaBgl1A and BaBgl3A. Furthermore, optimal preparation conditions for these rare ginsenosides were elucidated, with yields exceeding 76.34%. Additionally, large quantities of rare ginsenosides were prepared to determine their structures. Characteristic absorption peaks in 13 C NMR spectra and ion peaks in MALDI-TOF-MS suggested that the six high-purity rare ginsenosides (i.e., F2, CO and C-Mc1, CK, CY, and C-Mc) could be produced at high-efficiency. The findings of this study offer insights into novel enzymolysis methods for the targeted preparation of rare PPD-type ginsenosides, thus widening the range of raw materials that could be directed to in-depth biological activity research.
研究领域:
生物化学
·
药理学
·
生物技术
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DOI
高影响因子
Polymer Chemistry
·
2022
·Vol. 13
·期 27
·4054-4063
·
IF 5.364
Tianxiao Chang,Hongyi Qi,Chenxi Liang,Yanbin Huang,Wantai Yang
Benzothiophene is an under-explored monomer for free radical polymerization. Here we report the preparation of poly(benzothiophene-alt-maleic anhydride) (PBTMA) microspheres with high yield by self-stabilized precipitation polymerization. The chemical structure of the PBTMA copolymer was characterized by 1H-NMR, 13C-NMR, FT-IR and MALDI-TOF MS analyses. The effects of various reaction parameters, including the reaction medium, the monomer concentration, the initiator concentration and the reaction time, on the morphology, particle size and molecular weight (Mn) of the resultant PBTMA microspheres were investigated systematically. The experimental results demonstrated that the PBTMA copolymer was an alternating copolymer, whose composition was independent of the monomer composition. Uniform PBTMA microspheres with the diameter in the range of 400 nm to 3000 nm and Mn up to 12 000 g mol−1 were easily prepared. PBTMA had good solubility in common polar organic solvents and possessed much higher thermal stability than other maleic anhydride copolymers. PBTMA also showed interesting fluorescence properties both in the solid state and in solution. This research has laid the foundation for the further development of PBTMA copolymers as a new material.
研究领域:
高分子科学
·
材料科学
·
化学工程
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 1
·123-130
·
IF 3.288
Wei Gao,Pingping Jiang,Qian Gu,Hong Zhang,Pingbo Zhang,Agus Haryono
A novel green plasticizer, acetylated lactic acid 1,6-hexane diester (ALHD), was successfully synthesized based on L-lactic acid, a renewable organic acid from corn starch. Its chemical structure was characterized using FTIR, 1H NMR and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The plasticizing effects of ALHD as an alternative substitute for commercial plasticizer acetyl tributyl citrate (ATBC) in poly(vinyl chloride) (PVC) were investigated in detail. The results show that the PVC films plasticized using ALHD have better organic solvent resistance and food mimic migration stability than ATBC. After 24 hours of extraction in cyclohexane and petroleum ether, the weight loss of PVC sample S5 plasticized using pure ALHD was reduced by 83.6% and 77.5%, respectively, compared to that of PVC sample S1 plasticized with pure ATBC. Furthermore, after 72 hours of migration in activated carbon, the weight loss of PVC sample S5 decreased by 44.0% compared to that of PVC sample S1, which indicated that ALHD had excellent volatility and migration stability. The elongation at break of the PVC pieces increased from 644.65% to 802.15% as the content of ALHD improved, showing an increase of 24.4%. The mechanical property analysis showed that, with the replacement of ALHD in the place of ATBC, the PVC blends exhibited better flexibility and elasticity.
研究领域:
材料科学
·
化学
·
环境科学
·
高分子科学
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产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Jiang Bin,Zhang Xiaoqing,Yuan Yongqiang,Qu Yuxiao,Feng Zhibiao
An aqueous two-phase system (ATPS) consisting of poly(ethylene glycol-ran-propylene glycol) monobutyl ether (UCON)/phosphate was developed for the separation of the antioxidant peptides from pepsin hydrolysate of Whey Protein Isolate (WPI). The efficiency of the separation was evaluated based on the DPPH radical scavenging activity, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of the separated peptides. The effects of some parameters on the partition of antioxidant peptides were investigated. An efficient separation of antioxidant peptides was achieved using ATPS with pH of 4.0, 4 mL of UCON solution (40%, w / w ), 4 mL of KH 2 PO 4 solution (15.5%, w / w ), 2 mL of WPI hydrolysate and 0.40 g/10 mL of NaCl. Reversed-phase high-performance liquid chromatography (RP-HPLC), amino acid analyzer and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the purified peptides separated by the ATPS. The peptides in top phase were less polar than those in bottom phase. More antioxidative and hydrophobic amino acids were extracted to the top phase of ATPS, and the peptides with the amino acid sequences with antioxidant activities moved to the top phase as well. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by UCON/phosphate ATPS.
研究领域:
生物化学
·
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）