JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
·
IF 3.1
Yingxia Guo,Meichen Liu,Yajie Cheng,Jiye Tian,Chunpeng Feng,Qingbin Wang,Xuan Zhao,Lei Yin
Methoxy-polyethylene glycol amine (mPEG-NH₂), an important pharmaceutical excipient, has been extensively utilized in the field of biomedicine. To advance the development of mPEG-NH₂-related drug delivery systems, it is crucial to understand the safety profile and cellular pharmacokinetic behavior of mPEG-NH₂ polymers. In this study, a straightforward analytical assay based on ultra-high performance liquid chromatography and tandem mass spectrometry (UHPLC-MS/MS) for quantifying mPEG 5 -NH₂ in biological matrices was established and validated. Multiple reaction monitoring (MRM) transitions were selected for quantification of mPEG 5 -NH₂ (mass-to-charge ratio, m/z 252.1 → 87.7) and octaethylene glycol (OH-PEG 8 -OH) (mass-to-charge ratio, m/z 371.2 → 89.2). The UHPLC-MS/MS assay demonstrated excellent linearity within the concentration range of 0.01–10 μg/mL, with an R² value of 0.9996. Both intra-day and inter-day accuracies and precisions of the analytical method were within ± 9.19 %. This analytical assay was successfully applied to study the in vitro cellular toxicity and uptake behavior of mPEG 5 -NH₂ in MCF-7 cells. The results indicate that high doses of mPEG 5 -NH₂ may have potential toxicity to MCF-7 cells.
研究领域:
药物分析
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毒理学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
·
IF 4.4
Xinpeng Zhang,Xinyu Li,Danfeng Wang,Tianxin Weng,Lu Wang,Lixia Yuan,Qingpeng Wang,Jie Liu,Yushu Wu,Min Liu
Chemotherapy–phototherapy (CTPT) combination drugs co-loaded by targeted DNA nanostructures can achieve controlled drug delivery, reduce toxic side effects and overcome multidrug resistance. Herein, we constructed and characterized a DNA tetrahedral nanostructure (MUC1-TD) linked with the targeting aptamer MUC1. The interaction of daunorubicin (DAU)/acridine orange (AO) alone and in combination with MUC1-TD and the influence of the interaction on the cytotoxicity of the drugs were evaluated. Potassium ferrocyanide quenching analysis and DNA melting temperature assays were used to demonstrate the intercalative binding of DAU/AO to MUC1-TD. The interactions of DAU and/or AO with MUC1-TD were analyzed by fluorescence spectroscopy and differential scanning calorimetry. The number of binding sites, binding constant, entropy and enthalpy changes of the binding process were obtained. The binding strength and binding sites of DAU were higher than those of AO. The presence of AO in the ternary system weakened the binding of DAU to MUC1-TD. In vitro cytotoxicity studies demonstrated that the loading of MUC1-TD augmented the inhibitory effects of DAU and AO and the synergistic cytotoxic effects of DAU + AO on MCF-7 cells and MCF-7/ADR cells. Cell uptake studies showed that the loading of MUC1-TD was beneficial in promoting the apoptosis of MCF-7/ADR cells due to its enhanced targeting to the nucleus. This study has important guiding significance for the combined application of DAU and AO co-loaded by DNA nanostructures to overcome multidrug resistance.
研究领域:
纳米技术
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药学
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癌症研究
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分子生物学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACOLOGICAL SCIENCES
·
2014
·
IF 2.114
Jing Lu,Duanping Sun,Si Gao,Ying Gao,Jiantao Ye,Peiqing Liu
Autophagy is a highly regulated and multi-step biological process that serves to remove damaged cytoplasmic components and organelles. It has been suggested that the activation of autophagy may be a promising therapeutic strategy for cancer treatment by triggering cell death. In this study, we reported that cyclovirobuxine D (CVB-D), an alkaloid component in a traditional Chinese herb, could induce autophagy in the MCF-7 human breast cancer cell line. CVB-D inhibited the viability of MCF-7 cells in a concentration- and time-dependent manner. Activation of autophagy was characterized by transmission electron microscopy, monodansylcadaverine staining, and expression of autophagy marker microtubule-associated protein 1 light chain 3 (LC3). After CVB-D treatment, a clear accumulation of autophagosomes was observed accompanied with elevated LC3 fluorescent puncta. Western blot analysis revealed that CVB-D significantly promoted the conversion from LC3-I to LC3-II and the expression of autophagy-related protein 5 (ATG5), which are both essential for autophagosome formation. On the other hand, CVB-D–induced autophagy and decrease in cell viability could be blocked by 3-methyladenine, a well-established autophagy inhibitor. Moreover, CVB-D attenuated the phosphorylation of Akt and mTOR, two pivotal suppressors in autophagy pathways. These findings shed new light on the pharmacological actions and mechanism of CVB-D and may support the potential utility of autophagy inducers in cancer treatment.
研究领域:
肿瘤学
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Selvaraj Muthusamy,Kanagaraj Rajalakshmi,Qingxiang Xu,Yan Chen,Long Zhao,Weihua Zhu
Detection of H 2 S to analyze some diseases in living lives demands fast response, high selectivity and biocompatibility. Here we designed an azide containing coumarin attached with 8-aminoquinoline via amide backbone (ACAQ) fluorophore as the H 2 S sensing probe. Excellent response time of 6 min, high sensitivity with the limit of detection (LOD) of 14.6 nM and high selectivity with other possible interferences are revealed for ACAQ after characterized by spectroscopy, 1 H NMR titration and LC–MS measurements. The sensing strategy is explained by amide-iminol tautomerism and azide reduction. In addition, the successful visualization measurement suggests the practicability of the probe ACAQ for H 2 S detection in live samples.
研究领域:
生物化学
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医学诊断
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分析化学
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产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2021
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IF 4.06
Xu Xiao,Xiaolei Hou,Wenda Shi,Chanchan Hu,Yanan Cui,Jiuli Hu,Zongfang Piao,Xiaoqin Zhu,Qingshan Li,Fan Xu
Objective: Oxymatrine has shown strong anti-cancer ability, but its mechanism is not well-studied. Methods: The inhibitory rates of oxymatrine with various concentrations (0, 1, 2, 4, 6, 8, 16, 32 mg/ml) on MCF-7 cells were detected by CCK-8. The effects of oxymatrine on the expression of miRNA-140-5P in MCF-7 cells were detected by real-time fluorescent quantitative PCR (RT-PCR). miRNA-140-5P mimics or NC mimics were transfected into cells using Lipofectamine 2000. Eventually, the cells were divided into control-group, drug-group, miRNA-140-5P mimics group, NC mimics group, and miRNA-140-5P mimics + drug group. Cell viability was detected by CCK-8 assay and apoptosis rate of each group were measured by using Flow cytometry. Western blot was carried out to detect the protein expression of TGFBR1 and FGF9. Results: Oxymatrine at various concentrations had conspicuous inhibitory effect on the proliferation of MCF-7 cells ( P<0.05 ), and the inhibitory effect of oxymatrine on MCF-7 cells showed both dose- and time-dependent manners. The relative expression of miRNA-140-5P in MCF cells was remarkably lower than that in MCF-10A. Oxymatrine could effectively promote the expression of miRNA-140-5P in MCF-7 cells, and the relative expression of miRNA-140-5P increased significantly with the increased dose of oxymatrine ( P<0.05 ). Both transfection of miRNA-140-5P mimics and oxymatrine treatment could reduce the proliferation of MCF-7 cells ( P<0.05 ), and the proliferation of cells in miRNA-140-5P mimics + drug-group was significantly lower than that of other groups ( P<0.05 ). Compared with the control-group, the protein expressions of TGFbR1 and FGF9 in low-dose, medium-dose and high-dose groups were dramatically decreased ( P<0.05 ), in a dose-dependent manner ( P<0.05 ). Conclusion: Oxymatrine inhibits proliferation and promotes cell apoptosis of breast cancer MCF-7 cells. The mechanism may contribute to the regulation of miRNA-140-5p and its target genes.
研究领域:
肿瘤学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
Microsystems & Nanoengineering
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2025
·Vol. 11
·期 1
·1-10
·
IF 9.9
Ma Yanmei,Li Xinhao,Hu Weikang,Ruan Muyang,Yang Ming,Chang Lingqian,Gu Hongri,Hu Chengzhi
Ferroptosis is an iron-dependent form of regulated cell death driven by phospholipid peroxidation and the accumulation of reactive oxygen species (ROS), holding significant importance for therapeutic applications via its induction or inhibition. Accurate detection of intracellular Fe 2+ and ROS is essential, as these molecules play essential roles in initiating and propagating ferroptosis. In this study, we present a novel electrochemical nanoprobe for real-time, highly selective detection of intracellular Fe 2+ . The nanoprobes are prepared by coating gold nanoparticles (AuNPs) and poly(3,4-ethylenedioxythiophene) (PEDOT) onto silicon carbide nanowires (SiC NWs), which are subsequently functionalized with ferrocenyl endoperoxide carboxylic acid (FDCA) and integrated with a liquid metal-filled glass nanopipette. FDCA is specifically synthesized to enable precise electrochemical detection of Fe 2+ with high selectivity (0.1 nM to 1 µM) and exceptional specificity. PEDOT and AuNPs can improve electrical conductivity and provide a versatile interface for further FDCA decoration. We use the nanoprobes to evaluate the intracellular change of Fe 2+ in MCF-7 breast cancer cells during erastin-induced ferroptosis. We observe a significant increase in intracellular Fe 2+ levels in MCF-7 cells undergoing ferroptosis, accompanied by a notable rise in ROS levels. These findings underscore the potential of this nanoprobe to enhance our understanding of the mechanism of ferroptosis in tumor development and as a potential treatment target.
研究领域:
生物化学
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细胞生物学
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肿瘤学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2023
·
IF 3.8
Chenglu Zhang,Shiru Nie,Libo Shang,Chang Liu,Yanpeng Zhang,Yang Zhang,Jinghao Guo
A dual responsive and “turn-on” fluorescent probe NapL with large Stokes shift (115 nm) and large fluorescence emission (585 nm) was designed, synthesized and investigated. NapL has high selective and suitable sensitive (LOD = 82.6 nM) towards Cu 2+ over a wide pH range (7.4–12.1) through a fluorescence “turn-on” mechanism and with rapid response to Cu 2+ (30 s) in complex environments. Furthermore, NapL is sensitive toward pH changes within range (7.4–2.1) with obvious "turn - on" effect (about 37- fold). The sensitive detection characteristics of Cu 2+ can be ascribed to the coordination performance of N and O atoms to Cu 2+ , whereas pH responsiveness is ascribed to the protonation of imino group. NapL has been made into convenient and low-cost pH test strip for detecting pH range (7.4–2.1), and it has also been successfully used for the detection of Cu 2+ in MCF-7 breast cancer cells under neutral conditions.
研究领域:
分析化学
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生物医学
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材料科学
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DOI
高影响因子
Phenomics
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2025
·
IF 6.2
Hu Hongye,Wu Yanzhi,Hong Jingwei,Wang Huiying,Zhang Xiaohua,Huang Guanli,Zhu Xiaofang
Breast cancer (BC) remains the predominant form of cancer among women. Arsenic trioxide (ATO), an element in traditional Chinese medicine, has shown potential for treating BC, particularly when combined with resveratrol. However, the exact mechanisms of their combined action are not fully understood. This study aims to clarify their combined mechanisms through network pharmacology and experimental validation. In vitro experiments confirmed that compared with either agent alone, the combination of ATO and resveratrol more effectively inhibited the production of estrogen receptor 1 ( ESR1 ), tumor protein P53 ( TP53 ), and v-AKT murine thymoma viral oncogene homolog 1 ( AKT1 ) in MCF-7 cells. Our findings indicate that the combination of ATO and resveratrol significantly promotes cell apoptosis by suppressing ESR1 , TP53 , and AKT1 . Thus, ATO and resveratrol together may offer a promising strategy for BC treatment.
研究领域:
肿瘤学
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药理学
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分子生物学
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中医中药
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癌症研究
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3242
·
IF 3.098
Hailing Tan,Ziyi Xing,Gong Chen,Xiaofei Tian,Zhenqiang Wu
YellowMonascuspigments can be of two kinds: Natural and reduced, in which natural yellowMonascuspigments (NYMPs) attract widespread attention for their bioactivities. In this study, the antioxidative and antibreast cancer effects of the water-soluble NYMPs fermented byMonascus ruberCGMCC 10910 were evaluated. Results showed that water-soluble NYMPs had a significantly improved antioxidative activities compared to the reduced yellowMonascuspigments (RYMPs) that were chemically derived from orange or redMonascuspigments. Furthermore, NYMPs exhibited a concentration-dependent inhibition activity on MCF-7 cell growth (p< 0.001). After a 48-h incubation, a 26.52% inhibition yield was determined with 32 μg/mL of NYMPs. NYMPs also significantly inhibited the migration and invasion of MCF-7 cells. Mechanisms of the activities were associated with a down-regulation of the expression of matrix metalloproteinases and vascular endothelial growth factor. Rather than being alternatively used as natural colorants or antioxidants, this work suggested that NYMPs could be selected as potential functional additives in further test of breast cancer prevention and adjuvant therapy.Keywords:natural yellowMonascuspigments;water-soluble;antioxidation;migration;invasion;MCF-7 cells
研究领域:
药理学
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肿瘤学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cancer Nanotechnology
·
2023
·Vol. 14
·期 1
·1-16
·
IF 5.7
Bai Zhiqiang,Zhao Lu,Feng Haidi,Xin Zhihui,Wang Chaoyu,Liu Zhixiong,Tian Maozhong,Zhang Haifei,Bai Yunfeng,Feng Feng
Background Ti 3 C 2 is a type of transition metal carbides and nitrides (MXenes) with high light-to-heat conversion efficiency property, which has been widely used in cancer treatment recently. In fact, active targeting delivery of MXenes nanomaterials with targeting molecule could enhance the therapeutic efficacy. However, targeted therapy of MXenes has not been further studied in the past. Aptamers (Apt) with excellent affinity and high specificity properties have been widely used as targeting tools. Predictably, the incorporation of Apt into Ti 3 C 2 nanomaterials will offer an unprecedented opportunity in the research fields of cancer targeted therapy. Results Transmembrane glycoprotein mucin 1 (MUC1) is overexpressed on the surface of MCF-7 cells, and MUC1 Apt (Apt-M) could target MCF-7 cells with high affinity and specificity. Here, a smart targeting nanotherapeutic system Ti 3 C 2 /Apt-M was fabricated, which could specifically recognize and enter in MCF-7 cells. Benefitting from the desirable targeted performance of Apt-M, MCF-7 cells completed the ingestion process of Ti 3 C 2 /Apt-Mf nanosheets within 4 h, and Apt-M facilitated the entry of the Ti 3 C 2 /Apt-Mf nanosheets into MCF-7 cells. Besides, Ti 3 C 2 /Apt-M nanosheets exhibited the potential as an outstanding photothermal agent (PTA) because of the photothermal performance inherited from wrapped Ti 3 C 2 nanosheets. As demonstrated, upon 808 nm laser irradiation, the Ti 3 C 2 /Apt-M nanotherapeutic system displayed a satisfactory antitumor effect by targeted photothermal therapy both in vitro and in vivo. Conclusion This study provides a new idea for the development of MXenes nanotherapeutic system with high active targeting performance. Graphical Abstract
研究领域:
纳米技术
·
癌症治疗
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Guimei Wang,Xiaoxue Jiang,Nanyan Fu
Adenosine 5′-triphosphate (ATP), as a “molecular currency” for intracellular energy transfer, not only participates in various physiological activities, but also involves in various pathological processes of living cells. Therefore, the establishment of a simple, rapid, sensitive and accurate ATP analysis method is of great significance for the study of its physiological functions. In this work, we rationally design a near-infrared phenylboronic acid group-functionalized dicyanovinyl squaraine ( SQ-PBA3 ), with which a supramolecular assembly was constructed for fluorescent imaging of ATP. In PBS buffer, SQ-PBA3 changes self-assembly when adding cetyltrimethyl ammonium bromide (CTAB) deriving fluorescence response of SQ-PBA3 to ATP with the maximum emission at 700 nm, due to the specificity of phenylboronic acid to diols and the multiple electrostatic interactions between SQ-PBA3 , ATP and CTAB molecules. Meanwhile, the probe was successfully applied to monitor intracellular ATP level in MCF-7 cells with high sensitivity and selectivity.
研究领域:
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2015
·Vol. 6
·期 15
·2945-2954
·
IF 5.52
Lei Xu,Lichao Liu,Feng Liu,Haibo Cai,Weian Zhang
Amphiphilic PNIPAM-b-PTPPC6MA block copolymers were first synthesized using porphyrin-containing monomers. The mono-hydroxylphenyl-triphenylporphyrin photosensitizer, TPP-OH, was modified into a porphyrin monomer, and it was further used to construct the amphiphilic PNIPAM-b-PTPPC6MA block copolymers using RAFT polymerization. PNIPAM-b-PTPPC6MA block copolymers can self-assemble into a plethora of morphologies ranging from spheres to vesicular aggregates and further to a single large vesicle. The PNIPAM-b-PTPPC6MA micelles exhibited a high singlet oxygen quantum yield. Moreover, confocal laser scanning microscopy (CLSM) and flow cytometry analysis confirmed that PNIPAM-b-PTPPC6MA micelles could effectively enhance the internalization rate in MCF-7 cells. According to the MTT assay, PNIPAM-b-PTPPC6MA micelles exhibited low dark toxicity and efficient PDT efficacy towards MCF-7 cells. Thus PNIPAM-b-PTPPC6MA block copolymers have the potential application for photodynamic therapy.
研究领域:
生物材料
·
肿瘤学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）