Analytical Methods
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2018
·Vol. 10
·期 42
·5112-5117
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IF 2.073
Yanru Qin,Jingfan Xie,Shuting Li,Changqun Cai,Xiaoming Chen,Guanqun Zhong,Chunyan Chen
Glycoproteins have extraordinary significance for the diagnosis of tumors and cancers. However, in untreated complex biological samples, highly selective and sensitive determination of glycoproteins is still a huge challenge. In this study, a boronate affinity surface molecularly imprinted resonance light scattering (RLS) sensor was constructed to detect glycoproteins. In the RLS sensor, 4-vinylbenzeneboronic acid, which was used as a functional monomer for molecularly imprinted polymers (MIPs), is pH sensitive and can reversibly bind to glycoproteins. In addition, 2-(dimethylamino) ethyl methacrylate was also applied as a pH-responsive component to prepare MIPs. The swelling/shrinking properties of the resultant MIPs are beneficial to rapidly remove template glycoproteins from MIPs. Combining the boronate affinity and MIPs, the RLS sensor can specifically recognize target glycoproteins from sample solutions and sensitively detect them. Under optimum conditions, the RLS sensor has a linear response to ovalbumin (OVA) in the range of 0.5–15 nM with a low detection limit of 0.13 nM. The RLS sensor has been successfully utilized for the detection of OVA in real human blood serum, with a good recovery of 94.0–104%. This approach will provide a promising way to detect glycoproteins in clinical diagnostics.
研究领域:
分析化学
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生物传感器
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临床诊断
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DOI
高影响因子
RSC Advances
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2015
·Vol. 5
·期 90
·73424-73433
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IF 3.84
Yingchun Wang,Ningwei Wang,Xiaoni Ni,Qianqian Jiang,Wenming Yang,Weihong Huang,Wanzhen Xu
In this study, molecularly imprinted technology, combined with fluorescence measurement and computer simulation, was used to detect contaminant p-nitrophenol. Seven molecular dynamics simulations of molecular imprinting prepolymerization systems were performed to optimize the imprinting shell. Results indicated that the system with a p-nitrophenol (4-NP): 3-aminopropyltriethoxysilane (APTES): tetraethylorthosilicate (TEOS) mole ratio of 8 : 8 : 12 led to the best stable template–functional monomer clusters and the hybrid SiO2 layer with CdS-like clusters on the surface of CdTe was synthesized by a simple reflux procedure. The prepared imprinted materials (CdTe@SiO2-MIPs) on the surface of silica contained CdTe nanoparticles (CdTe@SiO2) by surface imprinting and Stöber method polymerization were characterized by transmission electron microscopy, fluorescence spectroscopy, Fourier-transform infrared spectroscopy, ultraviolet–visible analysis and X-ray diffraction. A linear relationship between relative fluorescence intensity and the concentration of 4-NP was obtained with a limit of detection of 0.08 μmol L−1 and the imprinting factor (IF) was 2.23 which indicated that special binding sites with binding property to p-nitrophenol were created on the surface of the CdTe@SiO2-MIPs materials. Ultimately, the feasibility of the fluorescent materials was successfully evaluated through the analysis of 4-NP in tap water and lake water. The recoveries were above 97.3%.
研究领域:
材料科学
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分析化学
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环境科学
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计算化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Dan Qiao,Tong Zhai,Jing Wang,Jing-Min Liu,Qiliang Deng,Shuo Wang
Hemoperfusion adsorbents is of vital importance for the therapy of hyperbilirubinemia. However, developing hemoperfusion adsorbents with the satisfactory selectivity, sustainability and biocompatibility remains a global challenge. Here we proposed a novel hemoperfusion adsorbent based on molecularly imprinted polymers (MIPs) for online cleansing bilirubin. The adsorbent displayed a high specificity with the imprinting factor of 3.98. The adsorption capacity of the MIPs toward bilirubin was 135.67 mg g - 1 , and 86.88% of the equilibrium adsorption amounts could be reached within 3 h. The resulted MIPs could also specifically capture bilirubin from the mixed analogue molecules solutions and plasma. Compared with the clinical adsorbents, the resulted adsorbent exhibited the lower hemolysis rates (<1.35%), the higher cell viabilities (>97%) and the excellent anticoagulant property. Besides, the loss of human serum albumin and aromatic amino acid was largely reduced. Furthermore, the great stability of the adsorbent ensured its sustainable usage at least four times. These remarkable performances show the application promise for bilirubin cleansing, and this approach can easily transfer to prepare other hemoperfusion adsorbents via replacing the templates with desired targets.
研究领域:
生物医学工程
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材料科学
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生物技术
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医疗器械
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DOI
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Xiaoqing Wang,Huiyi Chen,Jihui Zhang,Hong Zhou,Xiangying Meng,Na Wang,Yishan Fang,Bo Cui
The detection of Escherichia coli ( E. coli ) is of great significance for the environment and human health. Herein, a photoelectrochemical (PEC) detection strategy based on molecularly imprinted polymers (MIPs) was proposed for the sensitive detection of E. coli . 4,4′,4″-Trinitrotriphenylamine (TPA-NO 2 ) was prepared using a simple nitration reaction. Subsequently, MIP films were polymerized on the surface of TPA-NO 2 using 1,3-dihydrothieno[3,2- d ]pyrimidine-2,4-dione as the functional monomer with the dual functions of specific recognition and sensitization. The linear range was 10–10 8 CFU/mL and the limit of detection was 10 CFU/mL. It showed favorable recoveries in real sample tests of milk, orange juice and tomato. Additionally, the ability of functional monomers to bind excellently with E. coli was verified using molecular docking techniques. This research provided broader possibilities for constructing MIPs-PEC sensors and analyzing the interaction mechanism between E. coli and functional monomers.
研究领域:
分析化学
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生物技术
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环境科学
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食品科学
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DOI
PubMed
高影响因子
TALANTA
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2021
·
IF 6.057
Fei Li,Xiaoxuan Li,Jie Su,Yijun Li,Xiwen He,Langxing Chen,Yukui Zhang
In this work, a novel strategy to prepare molecularly imprinted polymers (MIPs) functionalized magnetic carbon nanotubes (MCNTs) via a facile sol-gel polymerization by adopting Cu 2+ -mediating interaction was presented for selective recognition of celastrol (Cel), in the traditional Chinese medicines (TCM). Firstly, template Cel, 3-aminopropyltriethoxysilane (APTES) as monomer and Cu 2+ (co-monomer) were mixed to form a self-assembled pre-complex, in which Cu 2+ could coordinate with Cel. Meanwhile, APTES plays a role of bridge between APTES and Cel. Secondly, carboxyl modified MCNTs as substrate was added into the pre-complex solution. After that, a multi-step sol-gel polymerization process was occurred in the presence of tetraethylorthosilicate as cross-linker and acetic acid as catalyst. Finally, MIPs layer was formed on the surface of the MCNTs (Cel-MIPs@MCNTs) after the removal of template with methanol/acetic. The morphology and structure of Cel-MIPs@MCNTs was investigated by various characterization techniques. The adsorption performance of Cel-MIPs@MCNTs to Cel was illustrated by kinetic, isothermal and selective binding experiments. The results displayed that the Cel-MIPs@MCNTs possessed fast kinetic equilibrium time (40 s), high adsorption capacity (13.35 μg mg −1 ), good imprinting factor of 3.41, and high magnetic responsivity (44.38 emu·g -1 ), which can be used as an ideal adsorbent for rapid isolation and enrichment of target analytes. A selective and sensitive method based on Cel-MIPs@MCNTs coupling with HPLC was developed for Cel determination including a wide linear range (0.15–200 μg mL −1 ) with correlation coefficient of 0.9998, a low limit of detection (0.05 μg mL −1 ). Furthermore, the applicability of Cel-MIPs@MCNTs was demonstrated to isolate and determine Cel in TCM samples with satisfactory recoveries ranged from 84.47% to 91.5% (RSD<5.35%). The results revealed that Cel-MIPs@MCNTs offer great potential as an adsorbent for selective and efficient isolation of Cel from complex TCM samples.
研究领域:
材料科学
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分析化学
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药学
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中医药
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DOI
高影响因子
RSC Advances
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2020
·Vol. 10
·期 42
·25402-25407
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IF 3.119
Xiaodong Lv,Peng Gao
Based on the electron-transfer mechanism between the template and quantum dots (QDs), an optical sensor was structured. It is a phenol sensor, which has the room-temperature phosphorescence (RTP) property of Mn-doped ZnS QDs and high selectivity of molecular imprinted polymers (MIPs). On the surface of the silane modified Mn-doped ZnS QDs, the phenol sensor was prepared by double cross-linker precipitation polymerization in the absence of any stabilizer and additive. Double cross-linkers, divinylbenzene (DVB) and ethyleneglycol dimethacrylate (EGDMA), make a great contribution to the imprinted polymerization with hydrogen-bonding interaction. Then, as a result of the functional monomer, methacrylic acid (MAA), a carboxylic acid was grafted onto the surface of ZnS QDs:Mn@MIPs. Under optimal conditions, the phenol determination experiment had a linear range of 5.0 to 55 μmol L−1 with a correlation coefficient of 0.9984, and a high imprinting factor (IF) of 3.43. In addition, the prepared ZnS QDs:Mn@MIPs were successfully used to detect phenol in real water samples. Therefore, this work provided a highly selective and sensitive RTP probe for phenol determination.
研究领域:
分析化学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 17
·6992-7000
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IF 3.288
Tehseen Nawaz,Muhammad Ahmad,Jieying Yu,Shiqi Wang,Tianxin Wei
Novel bifunctional group monomer itaconic acid (IA) based molecularly imprinted polymers (MIPs) were successfully prepared with hydrogen bonding interactions by applying a facile combination of a reversible addition chain transfer mechanism (RAFT) and surface plasmon resonance (SPR). UV photo-polymerization in synergy with 2-methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid (TTCA) as a chain transfer reagent was employed for MIP film synthesis on an SPR sensor chip for the detection of progesterone using ethylene glycol dimethacrylate (EGDMA) as a cross linker. In addition, the ratio of template to bifunctional group monomer to cross linker was optimized (1 : 2 : 12) by real-time SPR monitoring. The modified surface of the biosensor was characterized by contact angle measurements, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The resulting itaconic acid based MIP (IA based MIP) biosensor showed a high adsorption capacity and excellent selectivity in comparison to other analogues and non-imprinted polymer (NIP) film. Through 8 adsorption–desorption cycles, the high recoverability of IA based MIP film was confirmed. These features suggested that IA based MIPs are ideal candidates for biosensor use owing to the bifunctionality of the monomer. Consequently, within the concentration range of 1 × 10−18 to 1 × 10−8 mol L−1, the coupling angle change of SPR versus the negative logarithm of concentration showed excellent linearity: R2 = 0.99. Based on a linear equation, IA based MIPs showed excellent values for the limit of detection (LOD) and limit of quantification (LOQ), i.e. 0.28 × 10−19 mol L−1 and 0.92 × 10−19 mol L−1, respectively. Furthermore, the influence of the matrix on the biosensor was successfully analyzed in real samples with satisfactory outcomes, displaying identical behavior to ideal samples. Hence, IA based MIPs in combination with an SPR sensor chip demonstrated potential applications for rapid and highly effective sensing even in complicated matrices.
研究领域:
分析化学
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生物传感器
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高分子科学
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材料科学
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生物技术
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产品关联
DOI
高影响因子
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