CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Qinzhe Li,Yewei Zhang,Xuan Huang,Dongliang Yang,Lixing Weng,Changjin Ou,Xuejiao Song,Xiaochen Dong
Drug-resistant bacterial infection is a serious threat to the survival and health of human. Clinically, the conventional therapy for subcutaneous infections generally involves the systemic administration of high dose of antibiotics and frequently causes detrimental side effects in normal tissues. To overcome this issue, an acidity-responsive near infrared (NIR) light-triggered injectable hydrogel was designed for non-antibiotic local treatment. Herein, poly(ethylene glycol) diacrylate (PEGDA) was used as hydrogel matrix, polyoxometalate (POM) as acidity-enhanced photothermal agent and 2,2′-azobis[2-(2-imidazolin-2-yl) propane] dihydro-chloride (AIPH) as thermo-responsive initiator. After the hydrogel precursor solution (AIPH/POM/PEGDA) was injected into the acidic infected tissue, the aggregated POM with strong photothermal property was restricted to the site of abscess. Upon excitation at 1060 nm laser, the heat generated from POM would trigger alkyl radical formation from AIPH and thereafter achieving NIR-triggered in situ gel. Consequently, the POM and AIPH were trapped in Gel(AIPH/POM) and exhibited long-term retention in the abscess for further repeated local photothermal/thermodynamic antibacterial therapy. In vitro antibacterial evaluation indicated that the system could effectively eradicate drug-resistant bacteria through destroying bacteria integrity and producing the noxious alkyl radical under NIR light illumination. Moreover, the bacterial burden of abscess was significantly relieved and more collagens were deposited after the administration of Gel(AIPH/POM) and repeated NIR light. Meanwhile, no apparent adverse effect was observed during the treatment. This work not only provides a light-triggered gelation for local photothermal/thermodynamic synergistic therapy, but also offers a novel strategy for the rational design of light-responsive intelligent antibacterial hydrogels.
研究领域:
生物医学工程
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药学
·
材料科学
·
抗菌治疗
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光热治疗
·
智能药物递送系统
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 19
·4274-4286
·
IF 7
Jingge Zhang,Enna Ha,Danyang Li,Shuqing He,Luyang Wang,Shaolong Kuang,Junqing Hu
Nanozymes mediated catalytic therapy can produce toxic reactive oxygen species (ROS) and destroy the metabolic balance of tumor cells, providing a new direction for cancer treatment. However, the catalytic efficiency of a single nanozyme is limited by the complexity of the tumor microenvironment (hypoxia, GSH overexpression, etc.). In order to overcome these problems, we designed flower-like Co-doped FeSe2 (Co–FeSe2) nanozymes by a simple wet chemistry method. Co–FeSe2 nanozymes not only exhibit high POD and OXD-mimicking activities for facile kinetics, but also effectively consume over-expressed glutathione (GSH), inhibiting the consumption of generated ROS and destroying the metabolic balance of the tumor microenvironment. These catalytic reactions trigger cell death through apoptosis and ferroptosis dual pathways. More importantly, under the NIR II laser irradiation, the catalytic activities of Co–FeSe2 nanozymes are boosted, confirming the photothermal and catalytic synergistic tumor therapy. This study takes advantage of self-cascading engineering that offers new ideas for designing efficient redox nanozymes and promoting their clinical translation.
研究领域:
癌症治疗
·
材料科学
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纳米医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Chaoqun Li,Dong Gao,Yijian Gao,Ran Zhang,Xiongwei Qu,Shengliang Li,Chengfen Xing
Cell fate can be efficiently modulated by switching ion channels. However, the precise regulation of ion channels in cells, especially in specific organelles, remains challenging. Herein, biomimetic second near-infrared (NIR-II) responsive conjugated oligomer nanoparticles with dual-targeted properties are designed and prepared to modulate the ion channels of mitochondria to selectively kill malignant cells in vivo. Upon 1060 nm laser irradiation, the mitochondria-located nanoparticles photothermally release a specific ion inhibitor of the potassium channel via a temperature-sensitive liposome, thus altering the redox balance and pathways of mitochondria. NIR-II responsive nanoparticles can effectively regulate the potassium channels of mitochondria and fully suppress tumor growth. This work provides a new modality based on the NIR-II nanoplatform to regulate ion channels in specific organelles and proposes an effective therapeutic mechanism for malignant tumors.
研究领域:
纳米技术
·
癌症生物学
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诊疗学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·
IF 3.3
Qingyuan Cheng,Liman Li,Mingxia Yu
Silver chalcogenide (Ag2X; X = S, Se, Te) quantum dots (QDs) have facilitated the biomedical applications of QDs due to their low toxicity and tunable emission reaching the second near-infrared window (NIR-II window, 1000–1700 nm). The photoluminescence intensity of Ag2S QDs and Ag2Se QDs is considerably enhanced via Pb doping. However, the developed Pb-doped Ag2S QDs lacked in vivo verification, and the toxicity of the Pb-doped Ag2X QDs was only preliminarily assessed. Although QDs containing Pb have been widely introduced into the field of biomedicine, there are still concerns regarding toxicity associated with Pb. In the present study, we prepared polyethylene glycol (PEG)-modified Pb-doped Ag2S QDs with better biocompatibility and tested their in vivo bioimaging and toxicity in comparison with Ag2S QDs modified with the same PEG under identical concentrations. Results indicated that the PEG-modified Pb-doped Ag2S QDs significantly enhanced the brightness of in vivo bioimaging and spatial resolution for vessels inside the tumor without increasing toxicity, assessed based on cell viability post incubation for 96 h, intracellular ROS level, DNA damage, early apoptosis and mitochondrial injury. Our study demonstrated that the PEG-modified Pb-doped Ag2S QDs could serve as an advanced fluorescent probe for various biomedical applications.
研究领域:
生物医学工程
·
材料科学
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纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Nan Lv,Shaodong Zhai,Jun Xiong,Nan Hu,Xiang Guo,Zhida Liu,Ruiping Zhang
Pathological thrombus can cause serious acute diseases that present a significant threat to human health, such as myocardial infarction and stroke. Challenges remain in achieving effective thrombolysis and real-time monitoring of therapeutic effects while minimizing side effects. Herein,a multifunctional nanoplatform (TG-OPDEA@UK/MnO 2 -H1080) with enhanced thrombus-permeability was developed to monitor the therapeutic effect of antioxidant-thrombolysis by hydroxyl radical-responsive NIR-II fluorescence imaging. The polyzwitterion poly (oxidized N,N-Diethylaminoethyl methacrylate- co -n-butyl methacrylate) (OPDEA) was prepared as the matrix of nanoparticles to simultaneously loading urokinase (UK) and MnO 2 QDs, as well as NIR-II fluorescent molecule, H-1080. Subsequently, the fibrin targeted peptide CREKA was modified on the surface of the nanoparticles. OPDEA exhibits efficient loading capacity while endowing nanoparticles with the ability to effectively increased penetration depth of UK by 94.1 % into the thrombus, for extensive thrombolysis and fluorescence monitoring. The loaded UK exhibited good thrombolytic effect and greatly reduced the risk of bleeding by 82.6 %. TG-OPDEA@UK/MnO 2 -H1080 showed good thrombolytic efficacy and specific thrombus monitoring in the mouse carotid artery thrombosis model induced by ferric chloride (FeCl 3 ). This work prepares a nanoplatform for thrombolytic therapy and real-time efficacy assessment based on an independent externally forced thrombus penetration delivery strategy.
研究领域:
纳米技术
·
生物医学工程
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诊疗学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Mengxuan Du,Xiaojun He,Danyan Wang,Zhengting Jiang,Xiaoliang Zhao,Jianliang Shen
Deep tissue bacterial infections, especially methicillin-resistant Staphylococcus aureus (MRSA) infections, pose challenges to clinical therapy due to their low debridement efficiency and relapsing. Molybdenum disulfide (MoS 2 ) is used in the antibacterial field as a classic photothermal agent (NIR-I) with good biocompatibility. However, due to its limited NIR-I tissue penetration ability and single treatment mode, MoS 2 has poor therapeutic effects on deep tissue infection. Herein, we prepared a defect-type hybrid 2H-MoS 2 nanozyme (MoWS 2 ) using hydrothermal method fabricate the MoWS 2 composite, which is a new antibacterial strategy involving photothermal and enzyme catalysis, and further enhances the activity of the nanozyme through overheating. The regulation of 2H-MoS 2 defects through tungsten ion doping endows MoWS 2 with better near-infrared two-region absorption (NIR-II) and enzyme catalytic performance. Antibacterial activity experiments in vitro have shown that MoWS 2 can achieve efficient bactericidal activity and biofilm clearance through hyperthermia and reactive oxygen species (ROS). Deep MRSA infection experiments have shown that MoWS 2 rapidly removes bacteria from subcutaneous infected tissues through photothermal therapy (PTT) and chemodynamic therapy (CDT), accelerates the dissipation of abscesses, and promotes the healing of infected wounds. Additionally, the versatile treatment mode of MoWS 2 was further confirmed through tissue sectioning and immunofluorescence staining analysis. Overall, these results provide a feasible approach for achieving efficient treatment of deep tissue infections through tungsten ion doping to regulate defective 2H-MoS 2 . Statement of significance The photothermal effect of MoS 2 nanosheets in the NIR-I (650-900 nm) window in anti-MRSA therapy is considered to be highly reliable and efficient in PTA. However, most of the developed PPT therapies or antimicrobial systems based on PTT therapies developed with 1T-MoS 2 have in vivo sterilization temperatures of more than 55°C, which have the risk of damaging the normal tissues of the skin. In this study, we prepared W@MoS 2 with a good photothermal effect (36.9%) in the NIR-II window and good peroxidase-like activity. The combined effect of PTT and CDT has a stronger bactericidal effect while avoiding high-temperature damage, which makes the W@MoS 2 material more advantageous in terms of antimicrobial effect.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
传染病学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2022
·
IF 18.027
Mohammad Javad Afshari,Cang Li,Jianfeng Zeng,Jiabin Cui,Shuwang Wu,Mingyuan Gao
Bioluminescence (BL) imaging has emerged to tackle the potential challenges of fluorescence (FL) imaging including the autofluorescence background, inhomogeneous illumination over a wide imaging field, and the light-induced overheating effect. Taking advantage of the bioluminescence resonance energy transfer (BRET) mechanism between a conventional luciferin compound and a suitable acceptor, the visible light of the former can be extended to photons with longer wavelengths emitting from the latter. Although BRET-based self-illuminating imaging probes have already been prepared, employing potentially cytotoxic elements as the acceptor with the emission wavelengths which hardly reach the first near-infrared (NIR-I) window, has limited their applications as safe and high performance in vivo imaging agents. Herein, we report a biocompatible, self-illuminating, and second near-infrared (NIR-II) emissive probe to address the cytotoxicity concerns as well as improve the penetration depth and spatiotemporal resolution of BL imaging. To this end, NanoLuc luciferase enzyme molecules were immobilized on the surface of silver sulfide quantum dots to oxidize its luciferin substrate and initiate a single-step BRET mechanism, resulting in NIR-II photons from the quantum dots. The resulting dual modality (BL/FL) probes were successfully applied to in vivo tumor imaging in mice, demonstrating that NIR-II BL signals could be easily detected from the tumor sites, giving rise to ∼2 times higher signal-to-noise ratios compared to those obtained under FL mode. The results indicated that nontoxic NIR-II emitting nanocrystals deserve more attention to be tailored to fill the growing demands of preparing appropriate agents for high quality BL imaging.
研究领域:
生物医学工程
·
分子生物学
·
纳米技术
·
成像科学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2022
·
IF 10.508
Xiaonan Li,Siman Luo,Yu Chen,Yucheng Zuo,Yue Huang,Hong Zhang,Liheng Chen,Jun Feng,Jing-Yi Zhu,Wei Xue
Inspired by the effective interfacial interactions between natural creatures (e.g., cockleburs and viruses) and their bio-hosts, we developed a novel gold-manganese oxide nanoparticle (Au-MnOx) nanoenzyme with a spiky surface for NIR-II light-induced synergistic antibacterial therapy. Au-MnOx with favorable photothermal performance and peroxidase-like activity could achieve combined photothermal therapy (PTT)/chemodynamic therapy (CDT) under NIR-II irradiation with a low concentration. Of special note, the spiky surface endows Au-MnOx robust bacterial adhesion, in turn favoring the following synergistic PTT/CDT through effective interaction between Au-MnOx and bacteria. In vitro results display that our combined antibacterial nanoplatforms possess the broad-spectrum antibacterial ability against both Escherichia coli (E. coli, Gram-negative) and Staphylococcus aureus (S. aureus, Gram-positive). Moreover, satisfactory in vivo bactericidal efficacy and good cytocompatibility could be obtained in the rat wound model with the mixed bacterial infections. This study puts forward a facile one-pot method to establish the well-designed Au-MnOx nanozymes with a spiky surface for NIR-II light-enhanced bacterial elimination. The meteor hammer-like Au-MnOx holds great potential in construction of a novel antibacterial treatment system to settle the overuse of antibiotics.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
抗菌治疗
✓ 已验证
产品关联
DOI
高影响因子
Science Bulletin
·
2022
·
IF 20.577
Shaohua Qu,Qian Jia,Zheng Li,Zhongliang Wang,Li Shang
Near-infrared II (NIR-II) fluorescent nanoprobes hold great potential for biomedical applications. Elucidating the relationship between surface properties of NIR-II nanoprobes and their biological behaviors is particularly important for future probe design and their performance optimization. Despite the rapid development of NIR-II nanoprobes, the distinct role of surface chirality on their biological fates has rarely been exploited. Herein, chiral NIR-II fluorescent Ag 2 S quantum dots (QDs) are synthesized to investigate the relationship between their chirality and biological functions at both in vitro and in vivo levels. D- / L -Ag 2 S QDs exhibit significant differences on their interactions with serum proteins, which further affect the cellular uptake. As a result, D -Ag 2 S QDs can be internalized with higher efficiency (over 2-fold) than that of L -Ag 2 S QDs. Moreover, in vivo studies reveal that the chirality determines the primary localization of these chiral QDs, where a more efficient renal elimination of D -Ag 2 S QDs was observed than that of L -Ag 2 S QDs. Importantly, D -Ag 2 S QDs show preferential accumulation in tumor region than that of L -Ag 2 S QDs in orthotopic kidney tumor model, which points out a new avenue of enhancing targeting capabilities of nanoprobes by engineering their surface chirality.
研究领域:
生物医学工程
·
纳米技术
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2021
·Vol. 9
·期 16
·5542-5550
·
IF 6.843
Lan Fang,Jie Wang,Xueliang Ouyang,Binbin Liang,Liying Zhao,Dechun Huang,Dawei Deng
Multi-functionality has been a constant pursuit in the development of next-generation drug carriers, as it will bring the potential for combination therapy by integrating diverse therapeutic modes. In this work, FeSe2 nanosheets (NSs) have been prepared as a bifunctional platform to investigate their use in synergistic cancer therapy. Bifunctional FeSe2 NSs exhibit exceptional Fenton-like activity that generates cytotoxic hydroxyl radical (˙OH) and strong broad photothermal performance including the second-infrared (NIR-II) spectral range, wherein the ˙OH production can be enhanced by NIR-II light irradiation. Furthermore, doxorubicin (DOX) was conjugated onto NSs via a pH-responsive hydrazone bond to achieve preferential drug release in an acidic microenvironment. Upon intratumoral administration, these bifunctional drug-carrying FeSe2 NSs showed an NIR-II irradiation-reinforced strong tumor suppression effect, and no obvious toxicity to normal tissues was observed. This study provides a new paradigm for the design of advanced drug carriers relying on their inherent physicochemical properties.
研究领域:
纳米技术
·
癌症治疗
·
光热治疗
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Qing Hu,Fanyi Qin,Huijiao Qu,Huayu Xiong,Shengfu Wang,Xiuhua Zhang,Yan Xiao
Acute kidney injury (AKI) is a common kidney disease characterized by a sudden decline in renal function. It usually has no clear clinical symptoms in its early stages, but can easily progress to renal failure or even result in death. Real-time monitoring of AKI progression significantly enhances the prospects for early diagnosis and prompt intervention. However, there is no available to monitor AKI as developing a renal-clearable and reversibly responsive probe capable of long-term in vivo track biomarkers of AKI, such as reactive oxygen species (ROS), remains an extremely challenging task. Herein, we have constructed a redox-reversible second near-infrared (NIR-II) fluorescent probe based on Au nanoclusters (Au NCs) for long-term dynamic monitoring of AKI. The probe features NIR-II excitation and emission capabilities, along with effective kidney targeting and prolonged retention, facilitating renal enrichment and enabling long-term high-resolution renal imaging. Notably, it demonstrates rapid and reversible responsiveness to ROS with high sensitivity and selectivity, permitting real-time monitoring of redox fluctuations in vivo. Given its exceptional performance, the probe achieves unprecedented real-time dynamic tracking of AKI progression, providing crucial redox status information during the disease process. Additionally, the probe can serve as an effective new tool for screening of potential therapeutic drugs for AKI. This work may offer some substantial promotion for the diagnosis and treatment of kidney diseases.
研究领域:
生物医学工程
·
纳米医学
·
分子成像
·
肾脏病学
·
活性氧信号传导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Jiasheng Bao,Ruiqi Liu,Zhouyu Yu,Zhen Cheng,Baisong Chang
Despite multifunctional theranostics hold vast potential in deep tissue bioimaging and tumor therapy, activatable nanomedicine with integration of precise diagnosis and effective treatment is usually achieved at the cost of complicated synthesis chemistries. Here, a facile way to design bioresponsive Ag 2 S-Ag Janus probes coated by polyethylene glycol (PEG) (denoted as AAP) is showed, active in the second near-infrared window (NIR-II, 1000–1700 nm). In tumor microenvironment, Ag part can yield hydroxyl radicals (·OH) by consuming H 2 O 2 for high-efficiency chemodynamic therapy (CDT), while Ag 2 S part has impressive photothermal therapy (PTT). The synergistic benefits from Ag and Ag 2 S parts further boosted CDT effect of AAP Janus probes with high photothermal conversion efficiency up to 56.8%. Moreover, multiple lines of evidence supported that extremely faint fluorescence of AAP Janus probes can be significantly activated by overexpressed levels of H 2 O 2 , showing bright NIR-II emission at ≈1270 nm with over 5.6 × 10 3 -fold increase in signal intensity. AAP Janus probes are easily activatable for NIR-II imaging for tumor-specific identification, and effectively ablate tumor tissues with tumor inhibition rate of 96.2%. This study expects that AAP Janus probe will open up a new route to achieve precise tumor diagnosis and treatment in the NIR-II window.
研究领域:
生物医学工程
·
纳米技术
·
癌症治疗
·
生物成像
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）