JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 10
·4316-4326
·
IF 3.9
Youxin Qiu,Zhaoying Liu,Yuhao Mao,Weipeng Teng,Ming Li
A novel immunochromatography strip (ICS) based on the DNA-bridged double gold nanoparticles was established and evaluated for the dual-mode detection of ochratoxin A (OTA). For this purpose, the streptavidin was coupled with the big size of gold nanoparticle (40 nm, AuNP), the OTA monoclonal antibody and biotinylated DNA-SH were simultaneously immobilized on the small size of AuNP (20 nm), and then the enhanced ICS was self-assembled and evaluated. The dual-mode detection of semi-quantification and quantification had been achieved and performed by the proposed ICS. The LOD for semi-quantification (semi-Q-LOD) was 0.06 ng/mL by the directly naked eye (eightfold enhanced than conventional ICS). By the on-site reader, the LOD for quantification (Q-LOD) was 0.03 ng/mL (threefold enhanced), with the detection range between 0.03 and 1.2 ng/mL. The specificity, reliability, and practicability had been well represented. Furthermore, the OTA-positive results of the enhanced ICS method correlated well with those obtained by the referenced HPLC–MS/MS for the market samples. This study provided a new ICS pattern of semi-quantification and quantification for OTA contamination, which could be used as a valuable reference for improving the ICS technology and enhancing the sensitivity.
研究领域:
食品安全检测
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Xin Liu,Chunli Yan,Chunxiao Chang,Fansong Meng,Wenjie Shen,Song Wang,Yi Zhang
Background Mycotoxins, such as aflatoxin and ochratoxin A (OTA), are found at measurable levels in many staple foods; the health implications of long-term exposure of such toxins are poorly understood. Increasing evidence has confirmed the important role of OTA in upregulation of oxidative stress- and inflammatory response-induced tissue injury. However, it remains unknown whether ochratoxin A can promote chronic colitis and its associated colon cancer (CRC) development, and potential molecular mechanism. Additionally, RING finger-interacting protein with C kinase (RINCK) is a ubiquitin ligase and mediates immune response. Unfortunately, the potential molecular function of RINCK on regulation of colitis is still largely unknown. Purpose This study aims to provide mechanistic evidence that the role of RINCK in colitis and early colorectal cancer progression in response to OTA treatment via targeting nuclear factor erythroid 2-related factor 2 (NRF2). Methods The Cancer Genome Atlas (TCGA) database, GEO database, human subjects with CC phenotype and CC cell lines were used in this work. Pathological links between OTA, RINCK and treatment of CC are revealed through comprehensive means such as biological information analysis, clinical experiments, RNA-seq, and verification experiments. Results In this study, under oxidative stress in setting of colitis, we first identified RINCK as a key regulatory factor and a novel endogenous suppressor of nuclear factor erythroid 2-related factor 2 (NRF2), and we also confirm that RINCK is a NRF2 partner protein that catalyses its ubiquitination and degradation in intestinal epithelial cells (IECs). Notably, in vivo study, pathological phenotypes triggered by OTA pretreatment, accompanied by post-treatment of dextran sulfate sodium (DSS)-induced colitis was significantly mitigated by IEC-specific deficiency of Rinck , IEC- Rinck (KO) and adenovirus-associated virus (AAV)-triggered suppression of Rinck in rodent model, and lentivirus (LV)-mediated downregulation of Rinck (LV-sh Rinck ) in rabbit model, as determined by decreased endogenous reactive oxygen species (ROS) production, pro-inflammatory cytokines contents, improved body weights, reduced survival rates, restored colon length, assuasive DAI and histological scores. Inversely, transgenic mice by IEC-specific Rinck overexpression, IEC- Rinck (OE) accelerated colitis in acute or chronic colitis rodent models and in vitro experiments. Moreover, we found that OTA pretreatment-promoted azoxymethane (AOM)/DSS-induced colitis-associated early colorectal cancer (CRC) was also dramatically reduced by IEC- Rinck (KO), indicated by the decreased tumor number and corresponding KI-67 levels. Clinical samples analysis revealed that RINCK levels were greatly increased in tumor tissues of patients with CRC phenotypes. In parallel, RINCK deletion remarkably retarded the proliferation of colon cancer and tumor growth in vitro and in vivo , respectively. Mechanistically, in response to onset of colitis, RINCK directly interacts with NRF2 and promotes ubiquitin-proteasome degradation via increasing K48-linkage ubiquitin chain, thus leads in suppression of NRF2 nuclear translocation and its downstream cascade inactivation, which retards antioxidant defense. Conclusion The findings suggested that oral sub-chronic exposure of OTA significantly facilitates DSS-induced colitis and colitis-associated CRC development. These results further elucidated the potential role of RINCK in colitis progression by mediating NRF2 degradation, and could be considered as a therapeutic target for the treatment of such disease.
研究领域:
毒理学
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免疫学
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癌症生物学
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分子生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xingping Zhang,Yu Zhou,Hualin Yang,Wei Wei,Jing Zhao
In this work, a porphyrin metalation-based ratiometric absorbance and fluorescence dual model immunoassay was proposed to detect ochratoxin A (OTA). 5,10,15,20-tetrakis(1-methyl-4-pyridinio) porphyrin (TMPyP) was pink and had a strong fluorescence, upon coordination with Hg(II), its fluorescence was quenched and the color became green. The alkaline phosphatase can catalyze the dephosphorylation of ascorbic acid 2-phosphate to produce ascorbic acid, which can reduce the coordinated Hg(II) to Hg(0) and then dissociated from TMPyP, its fluorescence was recovered. Meanwhile, the color changed from green to light pink, which can be identified by naked eye for semi-quantitative detection. The linear ranges of ratiometric absorbance and fluorescence model were 0–6.0 ng/mL and 0.1–6.0 ng/mL, respectively. The absorbance and fluorescence signals produced by porphyrin metalation can mutually verify to improve the accuracy of detection results. Besides, the ultra-sensitivity and high selectivity demonstrated this method was a powerful tool for trace OTA detection.
研究领域:
食品科学
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分析化学
·
环境毒理学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Haixia Lyu,Haoran Sun,Yimen Zhu,Jun Wang,Zenghong Xie,Jinxia Li
In this study, a double-recognized aptamer-molecularly imprinted monolithic column (Apt-MIP monolithic column) was prepared by introducing both aptamer and MIP to reduce non-specific adsorption. Its preparation parameters such as the time of photo-initiation, the dosage of photo-initiator and the concentration of aptamer were investigated in detail. The recovery ratios of ochratoxin A (OTA) to ochratoxin B (OTB) on Apt-MIP monolithic column, Apt monolithic column and MIP monolithic column were 116.1, 40.8 and 69, respectively. Even if the concentration of OTB was 10 times that of OTA, the recovery of OTB was only about 2.9%. Applied to beer samples, the prepared Apt-MIP monolithic column drastically resisted background adsorption and the high-specificity recognition for OTA was obtained with the recoveries of 95.5–105.9%. This work provided a simple and effective method to selectively identify OTA from complex samples.
研究领域:
食品安全
·
分析化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Ming Lv,Haidong Gao,Shucai Gao,Yeru Liu,Jingjing Yao,Hongwei Liu,Fayun Wang,Mantang Chen,Lianjun Song,Tianlin Wang,Libin Wan
Ochratoxin A (OTA) contamination in food crops has sparked global concern for potential health risk to humans and animals. A simple, accurate and sensitive method was developed for the determination of OTA using electromembrane extraction (EME) coupled with ultra-high performance liquid chromatography-fluorescence detector (UPLC-FLD). By optimizing the important factors, EME showed good performance for the extraction of OTA in spiked corn flour sample. Using the developed EME-UPLC-FLD method, good linearity for OTA was achieved in the range of 1–100 ng mL −1 , and the limit of detection (LOD) was 0.02306 μg kg −1 , which was well below the established maximum residue limit. The proposed method was successfully applied for the determination of trace OTA in multiple matrices (corn flour extract, corn flour slurry and soybean milk) with satisfactory recoveries ranging from 80.01 % to 116.7 % at four spiked levels, which has great potential to analyze trace chemical contaminant in food crops.
研究领域:
食品安全
·
分析化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Wei Yang,Liang Feng
Background Surface immobilization of DNA is the foundation of a broad range of applications in biosensing and specific DNA extraction. Polydopamine (PDA) coatings can serve as intermediate layers to immobilize amino- or thiol-labelled molecules, including DNA, onto various materials through Michael addition and/or Schiff base reactions. However, the conjugation efficiency is limited by electrostatic repulsion between negatively charged DNA and PDA. Recently, it has been reported that polyvalent metal ions (such as Mg 2+ and Ca 2+ ) can mediate the adsorption of DNA on PDA surfaces. Inspired by this, in this work we aimed to exploit polyvalent metal ions to facilitate the conjugation of DNA on PDA. Results Mg 2+ was used to promote the conjugation of amino-terminated DNA complementary to ochratoxin A (OTA) aptamer (cDNA-NH 2 ) on PDA-coated magnetic nanoparticles (Fe 3 O 4 @PDA). After the reaction, the unlinked cDNA-NH 2 adsorbed on Fe 3 O 4 @PDA mediated by Mg 2+ was removed with EDTA. In the presence of 20 mM Mg 2+ , the amount of covalently linked cDNA-NH 2 increased approximately 11-fold compared to that in the absence of Mg 2+ . The resulting Fe 3 O 4 @PDA@cDNA conjugates exhibited superior hybridization capacity towards OTA aptamers, minimal nonspecific adsorption, and excellent chemical stability. The conjugates combined with fluorophore-labelled aptamers were employed for OTA detection, achieving a limit of detection (LOD) of 2.77 ng mL −1 . To demonstrate versatility, this conjugation method was extended to Ca 2+ -promoted conjugation of cDNA-NH 2 on Fe 3 O 4 @PDA nanoparticles and Mg 2+ -promoted conjugation of cDNA-NH 2 on PDA-coated 96-well plates. Significance The conjugation efficiency of DNA on PDA was significantly improved with the assistance of polyvalent metal ions (Mg 2+ and Ca 2+ ), providing a facile and efficient method for DNA immobilization. Due to the substrate-independent adhesion property of PDA, this method demonstrates versatility in DNA surface modification and holds great potential for applications in target extraction, biosensing, and other fields.
研究领域:
生物传感
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纳米技术
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Siqi Zhang,Kai-Bin Li,Yuanjiang Pan,De-Man Han
In this paper, an ultrasensitive nanochannel sensor has been proposed for label-free Ochratoxin A (OTA) assay in combination with graphene oxide (GO) and catalyzed hairpin assembly (CHA). The high-performance sensor is segmented into two parts. One is composed of graphene oxide (GO) and DNA probes . In the presence of target OTA, OTA works as a catalyst to trigger the self-assembly pathway of the two probes and initiate the cycling of CHA circuits, which results in numerous double-stranded DNAs (dsDNA) in solution. The excess ssDNA probes are removed by GO. The other part is composed of biomimetic nanochannel coated with polyethyleneimine (PEI) and Zr 4+ , which can quantify the concentration of OTA by detecting the dsDNA in solution. The nanofluidic device has a detection limit of as low as 6.2 pM with an excellent selectivity . The nanochannel based assay was used to analyse food samples (red wine) with satisfied results. Thus, the proposed analytical method will provide a new approach the detection of OTA and can be applied for quality control to ensure food safety.
研究领域:
食品科学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Ming Li,Yu Xiao,Zexuan Wang,Enze Sheng,Rujin Zhao,Chenfei Han,Daolin Du
Background Ultrasensitive detection is crucial for the early warning and intervention of risk factors, ultimately benefiting the environment and human health. Low levels of ochratoxin A (OTA) present a hidden yet significant threat, and rapid detection via high-performing biosensors is therefore essential. Results A cascade isothermal amplification aptasensor (CIA-aptasensor) was designed for OTA detection. On the surface of a magnetic bead probe, the OTA level was converted into positively correlated trigger cDNA through its competitive binding with OTA-Apt. The released trigger cDNA activated catalytic hairpin assembly followed by coupling with a hybridization chain reaction to achieve CIA. After adding graphene oxide and SYBR Green I, the background interference was eliminated to specifically obtain OTA-related fluorescence. The ultrasensitive limit of detection was 0.22 pg mL −1 , an improvement of 1368-fold over conventional enzyme-linked aptamer sorbent assay by the same OTA-Apt, demonstrating satisfactory reliability and practicability. Thus, the CIA-aptasensor provides an enzyme- and label-free simplified homogeneous system with minimal background interference using isothermal conditions. Significance This study provides a polymerase chain reaction–like approach for enhancing the sensitivity and performance of a biosensor, which could be extended for the application of CIA and label-free signaling strategy to other risk factors.
研究领域:
生物传感器
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毒理学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 66
·38590-38596
·
IF 3.049
Rong Huang,Lu Lu Xiong,Hui Hui Chai,Jing Jing Fu,Zhisong Lu,Ling Yu
A novel colorimetric aptasensor based on a Au/Fe3O4 nanohybrid was developed to detect ochratoxin A (OTA). The aptasensor is composed of a free OTA aptamer, a Au/Fe3O4 nanohybrid coated with biotinylated complementary DNA of the OTA aptamer (biotin-cDNA-Au/Fe3O4), and free alkaline-phosphatase-labeled streptavidin (SA-ALP). The Au/Fe3O4 nanohybrid not only immobilizes biotin-cDNA but also magnetically separates SA-ALP from the sample solution. One part of the OTA aptamer sequence hybridizes with biotin-cDNA immobilized on Au/Fe3O4, and the left part of the OTA aptamer sequence covers the biotin and blocks the specific interaction between biotin and SA-ALP. OTA can interrupt the interaction of OTA aptamer binding to biotin-cDNA-Au/Fe3O4 and can inhibit the shielding effect of the OTA aptamer on biotin. The amount of SA-ALP that can be captured by biotin-cDNA-Au/Fe3O4 thus increases with increasing OTA concentration. Through a simple magnetic separation, the collected SA-ALP-linked Au/Fe3O4 can produce a yellow-colored solution in the presence of p-nitrophenyl phosphate (p-NPP). This colorimetric aptasensor can detect OTA as low as 1.15 ng mL−1 with high specificity.
研究领域:
分析化学
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纳米技术
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毒理学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Xiangyu Sun,Yuxue Niu,Tingting Ma,Pingkang Xu,Weidong Huang,Jicheng Zhan
A simple and convenient HPLC-FD detection method for ochratoxin A (OTA) with a high detection limit and a short run time has been developed. OTA has been found in most samples of Chinese market wine, including domestic and imported wines, but the content was not very high. Only a few wines showed an OTA content that exceeded the EC and OIV limits, indicating that most Chinese market wines were safe. The OTA intake for Chinese from wine was 0.017 ng/kg (bw) per day, which was lower than the SCF and JECFA limits and also lower than in many other countries. This was mainly due to the low per capita wine consumption in China, but it is still necessary for the Chinese government and wine makers to monitor OTA levels in wine and to establish relevant regulations. An egg white treatment (0.20 mg/mL, 48 h) was the best removal method for OTA.
研究领域:
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoyu Shi,Xiaochun Zhu,Yaqin Chai,Ying Zhou,Ruo Yuan
Ochratoxin A (OTA) with high toxicity represents a serious threat to the agriculture and food chain, consequently to human health. Herein, a simple electrochemiluminescence (ECL) biosensor was constructed for ultrasensitive detection of OTA based on mercaptopropionic acid templated Au nanoclusters (Au NCs) as intensive signal probe and a non-enzymatic 2D DNA walking machine as the effective amplification strategy. Specifically, the target related bipedal DNA walker efficiently moved along 2D DNA tracks through toehold-mediated DNA strand displacement, which triggered abundant signal probes for combining to the DNA tracks. Moreover, the Au NCs could exhibit strong ECL emission due to fast electron transfer from massive Au-S electronic pathways under the electrochemical excitation. Thus, the biosensor possessed significant ECL response for achieving ultrasensitive detection toward OTA with low detection limit of 3.19 fg/mL. Impressively, the sensing platform was also applied to detect OTA from edible oils, exhibiting great application potential in food analysis.
研究领域:
分析化学
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食品科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1141
·
IF 5.743
Chunyuan Tian,Minggang Wei,Xiaobin Wang,Qing Hua,Feiyan Tang,Lijun Zhao,Xuming Zhuang,Feng Luan
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3nanocrystals with K2S2O8as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL−1to 10 ng mL−1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL−1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.Keywords:Gd(OH)3nanocrystals;electrochemiluminescence;aptasensor;ochratoxin A;food detection
研究领域:
分析化学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）