STAR Protocols
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2023
·
IF 0
Bo Zhou,Ye Chen,Lifang Li,Jiashuo Liu,Yuyan Wang,Zheying Huang,Zheng Hu,Rui Tian,Zhen Li
Summary Here, we present a protocol for generating LtCas12a protein recognizing distinct TTNA (N represented A, T, C, G) protospacer adjacent motif sequence. We describe steps for transforming and harvesting bacterial cells and protein purification including nickel affinity chromatography and dialysis. We then detail procedures for verification of LtCas12a with cis- and trans-cleavage activities. For complete details on the use and execution of this protocol, please refer to Chen et al. (2023). 1
研究领域:
分子生物学
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生物化学
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基因工程
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DOI
PubMed
CHEMOSPHERE
·
2020
·
IF 5.778
Jie Zheng,Xiaomin Tang,Shixin Zhang,Ting Huang,Huaili Zheng,Bin Sun
Chitosan-based flocculants are efficient and biodegradable, possessing the potential application in water treatment . A chitosan-based flocculant, CTS-g-PAM, was prepared via grafting copolymerization . Two main structures of CTS-g-PAM were detected by X-ray photoelectron spectroscopy (XPS), and they related to the different performances of CTS-g-PAM in the treatment of model azo dyeing wastewater in terms of removal efficiency, floc size and zeta potential . CTS-g-PAM with the optimal ratio of C N to –NH– owned the best performance. C N reinforced the interaction between CTS-g-PAM and azo dyes and charge neutralization while the flocs formed in the case were large and compacted. However, excessive C N accelerated itself hydrolysis and leaded to the decomposition of CTS-g-PAM, deteriorating the performance of the flocculant.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
Biotechnology Journal
·
2025
·Vol. 20
·期 12
·e70169
·
IF 3.1
Yiran Tang,Jie Xu,Wang Liu,Jingjing Zhu,Huan Fang,Langyu He,Hongxi Liu,Yuen Yee Cheng,Xiangqin Li,Huanwei Sun,Wei Zhang,Kedong Song
Cardiovascular diseases are a leading global cause of mortality, with cardiac patches (CPs) emerging as a novel surgical treatment. This study used a combined decellularization method with sodium dodecyl sulfate (SDS), Triton X-100, and DNase to process porcine myocardial tissue (PMT), yielding decellularized extracellular matrix (dECM). Quantitative analysis revealed that the dECM contained collagen, DNA, and glycosaminoglycans (GAGs) at concentrations of 2.63 ± 0.37 µg/mg, 4.27 ± 0.79 ng/mg, and 15.94 ± 0.60 µg/mg. Additionally, a conductive hydrogel patch (PDA/CSCA/PAM) with a uniform porous structure and excellent mechanical properties was developed. Its adhesive strengths on glass, stainless steel, and PMT were 50.71 ± 2.88 kPa, 34.19 ± 3.63 kPa, and 54.71 ± 3.24 kPa, respectively. Bacterial inhibition rates reached 103.26 ± 3.52% (Day 1) and 99.26 ± 5.35% (Day 3), indicating significant antimicrobial efficacy. The patch met myocardial tissue engineering standards for swelling ratio, hydrophilicity (contact angle <90°), hemolysis rate (<5%), and conductivity (10 −4 S cm −1) , with biosafety certified by ISO 10993-5. These results highlight its mechanical compatibility, antimicrobial activity, and biocompatibility, offering a multifunctional solution for myocardial infarction repair with high clinical translation potential.
研究领域:
心血管工程
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生物医学材料
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组织工程
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再生医学
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2024
·Vol. 31
·期 3
·1-13
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IF 2.6
Li Shuo,Tao Yulun,Wu Yuannan,zhu Shanshan,Khademi Sara,lv Yinru,Tai Yanlong,Wang Chaoran
The high integration and multifunctionality in flexible electronic device play an important role in its development. In this study, we developed multifunctional hydrogels integrated with supercapacitive, photoelectric, and strain-sensing capabilities. The hydrogel composite consists of PAM/PVA matrix with polyaniline (PANI) and reduced graphene oxide (rGO) as conductive components. Two different acid dopants, citric acid (CA) and hydrochloric acid (HCl), were employed to investigate their impact on photoelectrochemical capabilities of hydrogels. Both CA-doped and HCl-doped hydrogels exhibited remarkable supercapacitive performance, achieving areal capacitances of 492 mF/cm 2 and 538 mF/cm 2 , respectively. Furthermore, both hydrogels demonstrated photosensitivity towards shorter wavelengths such as ultraviolet (380 nm) and blue (475 nm) light. Lastly, the proposed hydrogels serving as highly sensitive strain sensors was verified through effectively sensing arm bending movements.
研究领域:
材料科学
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电化学
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光电子学
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传感器
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纳米技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Linyi Li,Xinglai Yuan,Yuxin Ma,Yuyang He,Linan Wang,Fan Zhang,Limeng Song,Rui Zhang,Bingbing Fan
Positively charged HFO and negative MXene were electrostatically assembled. • The interconnected porous Mxene/HFO/PAM hydrogel was precisely fabricated via DLP 3D printing. • The optimized absorber delivers ultrahigh RL min of −55.18 dB and broad EAB of 5.68 GHz. Architecturally engineered multifunctional hydrogels hold great promise for integrated electromagnetic protection and flexible electronics. However, achieving high-performance microwave absorption while maintaining structural flexibility and high-resolution printability remains a significant challenge due to impedance mismatch and unoptimized attenuation networks. Herein, a three-dimensional architectured MXene/HFO/PAM composite hydrogel was fabricated via electrostatic self-assembly and DLP 3D printing, yielding an integrated material system with efficient electromagnetic wave absorption and flexible strain-sensing capabilities. Positively charged HFO nanospheres were uniformly assembled onto negatively charged MXene nanosheets, constructing a hybrid dielectric-magnetic filler with improved interfacial compatibility. Combined with the three-dimensional interconnected porous architecture enabled by DLP printing, the optimized MHH-2 hydrogel achieved an RL min of −55.18 dB and an EAB of 5.68 GHz at a thickness of 2.7 mm. In addition, the integrated conductive framework and robust hydrogel network afforded excellent mechanical flexibility and stable strain-sensing behavior, with rapid response/recovery times of 200/350 ms and reliable motion-monitoring performance. This study offers a promising route toward architecturally engineered hydrogel-based integrated material systems for electromagnetic protection and intelligent sensing. Download: Download high-res image (209KB) Download: Download full-size image
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DOI
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
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2025
·Vol. 40
·期 2
·382-388
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IF 1.1
Cao Tian,Li Wei,Ren Lin,Song Tao,Song Jie,Lin Jiacheng,Yang Li,Liu Wenxiu,Wang Ping,Feng Shaojie
The adsorption of phosphate was conducted by the complex of chitosan/polyacrylamide/ferric(CS/PAM/Fe(III)) prepared. The SEM images and XPS spectra confirmed the successful adsorption of phosphate. The adsorption process was studied by varying the influencing aspects like pH, co-existing ions, contact time, and initial phosphate concentration. The experimental results indicate that the adsorptive capacity decreases with the increase of pH. However, it is commendable that there is still a adsorption capacity of more than 5 mg/g when the pH is 8–11. The adsorption kinetics can be accurately described by the pseudo-second-order model and is controlled by both chemisorption and surface diffusion. The adsorption process is a single layer adsorption. This paper proposed that the adsorption mechanism of CS/PAM/Fe(III) complex is the joint action of electrostatic attraction and ligand exchange.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Nano Energy
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2024
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IF 16.8
Furong Cao,Jingchang Sun,Siwen Liu,Huiying Cheng,Zijiang Yang,Liuting Shan,Zhan Wang,Qiuju Feng,Jiming Bian,Junfeng Gao
Moisture electricity generator (MEG) has gained increasing interest due to its tremendous potential applications in generating electricity from sustainable ambient moisture. However, the development of MEG for self-powered electronics is seriously hindered by the low power density of individual MEG devices. Moreover, it is highly desirable to explore the potential of MEG in wearable applications. To significantly enhance the output performance of wearable MEGs, an ionic hydrogel moisture electricity generator (IH-MEG) featuring a PAM-LiCl/CMC structure was fabricated, demonstrating an excellent combination of flexibility and high-power density. The moisture energy is produced from the phase transition of water between its gaseous state and its adsorbed state, which exhibit different enthalpy and entropy. A significant number of ions are generated upon water absorption, and the three-dimensional pore structure facilitates the rapid transport of these ions within the IH-MEG, effectively converting the chemical energy stored in the IH-MEG into electrical energy. Under normal conditions (20°C and 90 % relative humidity), the maximum output power density from an individual IH-MEG can reach as high as 113 μW·cm⁻², with an open-circuit voltage of 1.8 V and a short-circuit current of 1.2 mA, nearly doubling the previously reported values. Moreover, our IH-MEG can collect mechanical energy when pressure is applied to the device. Our achievements provide profound insights into enhancing the output performance of sustainable MEGs through the rational utilization of ion hydrogels via a simple fabrication process.
研究领域:
材料科学
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能源技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Jiani Xu,Tingwei Cai,Hanchang Hu,Chen Meng,Xia Dong,Qiangqiang Zhao,Jinxin He
This work primarily discusses the construction of physically cross-linked polyacrylamide copolymer containing 2-(3-(6-methyl-4-oxo-1,4-dihydropyrimidin-2-yl)ureido)ethyl methacrylate (PAM-co-UPyMA, short for PAU) hydrogels with topological entanglement by designing high concentrations of acrylamide (AM) monomers and introducing “bridging” flexible non-covalent bonds. This method overcomes the poor stretchable performance of chemically cross-linked hydrogels, as well as the limited improvement in mechanical properties due to the low dissipation energy of physically cross-linked hydrogel (non-covalent bonding) under an external force. This new hydrogel exhibits low modulus (0.12 MPa), a certain fracture strength (0.2 MPa), and high toughness with an elongation at a break of 5700 %, demonstrating excellent tensile strain sensitivity with a gauge factor (GF) of 16.14, capable of detecting tiny deformations of 0.2 %. It also exhibits fast strain response, long-term stability, and performs well in applications such as human joints and voice monitoring. Furthermore, this work elucidates the relationship between the microstructure of PAU hydrogels and sensing performance, proving that the rate of microstructure change serves as an essential mechanism. The less cross-linked and highly entangled PAU hydrogel strain sensor increases the cross-linked domain size under tensile load, leading to ion channel blockade with a rapid increase in resistance during deformation, thus resulting in an improved sensitivity.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 24
·25286-25300
·
IF 2.914
Liu Fang,Che Yangli,Chai Qingwen,Zhao Mengfei,Lv Yan,Sun Hui,Wang Yongqiang,Sun Juan,Zhao Chaocheng
Copper and zinc composite oxides (Cu 2 O/ZnO) were synthesized by an impregnation-reduction-air oxidation method. A series of Cu 2 O/ZnO/rGO ternary composites were prepared by coupling with graphene oxide (GO) with different mass fractions in a solvothermal reaction system. The microscopic morphology, crystal structure, and optical characteristics of the photocatalysts were characterized. The degradation of p-Nitrophenol (PNP) and polyacrylamide (PAM) by photocatalytic materials under simulated solar irradiation were studied, and the degradation kinetics were also investigated. The results showed that cubic Cu 2 O was modified by ZnO nanorods and distributed on rGO nanosheets. The ternary Cu 2 O/ZnO/rGO nanocomposites have stronger simulated solar absorption ability and higher photodegradation efficiency than pure ZnO and binary Cu 2 O/ZnO nanocomposites. When the amount of Cu 2 O/ZnO/rGO-10 was 0.3 g L −1 , the degradation rate of 10 mg L −1 PNP reached 98% at 90 min and 99.6% of 100 mg L −1 PAM at 30 min. The photocatalytic degradation processes of PNP and PAM all followed the pseudo-first-order kinetic model. Free radical trapping experiments showed that superoxide radicals were the main active substances to improve photocatalytic efficiency. In addition, after four recycles, the catalytic efficiency of Cu 2 O/ZnO/rGO-10 was still over 90%. It showed that Cu 2 O/ZnO/rGO-10 was a promising catalyst for wastewater treatment because of its good photostability and reusability.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2017
·Vol. 32
·期 6
·970-977
·
IF 1.509
Shujuan Yu,Kuan Chen,Feng Wang,Yongfei Zhu,Xuehong Zhang
A simple microwave-assisted solvothermal method was used to prepare fluorescent nitrogen-doped carbon dots (N-CDs) with high fluorescence quantum yield (79.63%) using citric acid and N -(2-hydroxyethyl)ethylenediamine as starting materials. The PVAm-g-N-CDs grafted products were synthesized by amide bond formation between the carboxylic groups of N-CDs and amine groups of polyvinylamine (PVAm). Fluorescent hydrogel films (PVAm-g-N-CDs/PAM) were synthesized by interpenetration polymer network polymerization of PVAm-g-N-CDs and acrylamide (AM). When used for ion detection, we found that the fluorescence of the hydrogel films was clearly quenched by addition of Hg 2+ . Repeatability tests on using the hydrogel films for Hg 2+ detection showed that they could be applied at least three times. The PVAm-g-N-CDs/PAM could serve as an effective fluorescent sensing platform for sensitive detection of Hg 2+ ions with a detection limit of 0.089 μmol/L. This work may offer a new approach for developing recoverable and sensitive N-CDs-based sensors for biological and environmental applications.
研究领域:
材料科学
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环境科学
·
分析化学
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 27
·19174-19184
·
IF 3.9
Peixin Hu,Hechao Lu,Qinting He,Fang Ren,Juan Wu,Wei Jiang
In this work, intelligent pH-sensitive sensors (Fe3O4/RhB@PAM) for Cr(VI) detection were successfully synthesized based on polyacrylamide (PAM) and Rhodamine B (RhB) co-modified Fe3O4 nanocomposites. The characterization results indicated that the sensors had many favorable properties, including suitable size, stable crystal structure and excellent magnetic response performance (47.59 emu g−1). In addition, the fluorescence changes during the detection process indicated that Fe3O4/RhB@PAM were “ON–OFF” intelligent sensors. When the Fe3O4/RhB@PAM sensors were placed in acidic Cr(VI) solution (pH 4), PAM acted as a pH-responsive “gatekeeper” releasing RhB, and the fluorescence intensity of released RhB was weakened by the complexation of Cr(VI). Furthermore, the fluorescence changes of the magnetic sensors were remarkably specific for Cr(VI) even in the presence of other competitive cations, and the limit of detection (LOD) for Cr(VI) was lower (0.347 μM) than the value recommended by the World Health Organization (0.96 μM). All the results presented in this study showed that the Fe3O4/RhB@PAM sensors had significant potential for Cr(VI) detection in acidic environmental samples.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lingqi Deng,Ziqi Xu,Fang Wang,Jiaqi Liu
Natural polysaccharide polymer hydrogels have garnered significant attention in biomedical applications due to their exceptional biocompatibility and biodegradability. However, their practical utility is constrained by inherent limitations such as inadequate mechanical strength and poor toughness. These deficiencies often result in premature drug release and reduced functional longevity when applied in drug delivery systems. To address these challenges, the incorporation of self-healing systems based on dynamic covalent or non-covalent bonds has emerged as an effective solution. In this study, chitosan (CS) was first quaternized (marked as QCS) and then compounded with gelatin and polyacrylamide, using 5-fluorouracil (5-Fu) as the model drug. The Fe 3+ @ TA system, formed by adding Fe 3+ and TA, served as a redox initiator to enable free radical polymerization of acrylamide and shorten the preparation time of hydrogel. Ultimately, a smart hydrogel with an interpenetrating network structure was successfully prepared. By adjusting the concentrations of CS and TPP, the regulation of the microstructure and physicochemical properties of the hydrogel was achieved. The results showed that the hydrogel, as a candidate multifunctional dressing, possessed excellent tensile and compressive properties, self-healable capability, and appropriate repeatable adhesion performance. In vitro drug release and antimicrobial assays demonstrated that the hydrogel exhibits pH-responsive release characteristics and effective antimicrobial properties against Staphylococcus aureus and Escherichia coli . Comprehensive biocompatibility assessments, including cytotoxicity and hemolysis testing, confirmed the material's biological safety profile. These findings collectively establish the strong and flexible composite hydrogel as a promising platform with extensive potential for drug delivery applications.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）