JOURNAL OF MICROENCAPSULATION
·
2019
·
IF 2.04
Yingting Wang,Along Zuo,Xiaoyan Huang,Ying Ying,Xinsheng Shu,Xianxiong Chen,Yatao Yang,Junxian Ma,Guimiao Lin,Xiaomei Wang,Lin Mei,Gan Liu,Yingying Zhao
Taxane-based chemotherapy-loaded drug delivery systems have great potential for cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical cancer cell line Hela and the human breast cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and ‘self-controlled’ drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast cancer cells and human cervical cancer cells.
研究领域:
药剂学
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肿瘤学
·
纳米技术
·
药物递送系统
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癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 50
·44865-44872
·
IF 3.289
Yazhen Niu,Junlin He,Yuliang Li,Yilin Zhao,Chunyong Xia,Guolin Yuan,Lei Zhang,Yuchan Zhang,Chao Yu
Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. α2,3-Sialylated glycans and α2,6-sialylated glycans are the predominant sialic acids found in nature. Different expression of the quantity of glycans can result in development of different disease. However, there are no ideal methods for discriminating α2,3-sialylated glycans and α2,6-sialylated glycans. In this work, a multi-purpose biosensor is fabricated for sensitive detection of α2,3-sialylated glycans and α2,6-sialylated glycans. To improve the sensitivity of the biosensor, p-MWCNTs were integrated with PAMAM, as PAMAM has highly branched and abundant amino groups, providing a large available surface area for linking with other substances. To achieve distinguishable recognition, Maackia amurensis lectin (MAL) and Sambucus nigra agglutinin (SNA) were included. To facilitate the lectin fixation, PDITC, a kind of green homobifunctional cross-linker, was selected. Under optimized detection conditions, the linear range of detection for α2,3-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a lower detection limit of 3 fg mL−1, and the linear range of detection for α2,6-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a detection limit of 3 fg mL−1. This work not only provides a method for distinguishing detection of α2,3-sialylated glycans and α2,6-sialylated glycans, but also provides a reference for future clinical testing.
研究领域:
生物化学
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分析化学
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生物医学工程
·
临床诊断
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2021
·
IF 2.991
Minjun Ma,Xueke Gao,Zhaohui Guo,Yan Qiao
Biotin–dendrimer conjugates (such as biotin-PAMAMs-NH2) are important macromolecules in the field of host–guest chemistry and widely used systems for delivery. The similar chemical structures of the inner and outer layers of PAMAM-NH2 make it difficult to illuminate the interaction and the binding affinity of biotin-PAMAMs-NH2. By utilizing NMR techniques including 1H NMR titration, CSSF-TOCSY, STDD methods, and 2D DOSY analysis, we demonstrate a method to sort out these interactions. The methylene protons of the inner and outer layers of PAMAM-NH2 are successfully identified and accurately positioned so that the carboxylic acid groups of biotins are having ionic interactions with the outermost amine groups of PAMAM-NH2. The inner PAMAM-NH2 is protonated when reaching the isoelectric point of PAMAM-NH2, increasing the hydrodynamic radius. On the basis of the NMR experiments, a model is proposed, where the carboxylic acid groups and heterocyclic skeleton of biotin arched over the outer layers of PAMAM-NH2 like a bridge. Furthermore, using STDD epitope mapping, the binding affinity between biotin and PAMAM-NH2 was quantified. The diffusion behavior of biotin-G5 PAMAM-NH2 complex is more complicated than that of biotin-G3 PAMAM-NH2 complex due to steric hindrance. The results provide a theoretical basis for understanding these complicated drug delivery systems.
研究领域:
化学
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药物递送系统
·
分子相互作用
✓ 已验证
DOI
高影响因子
Silicon
·
2017
·Vol. 10
·期 2
·483-493
·
IF 0.829
Wen Zhao,Jincheng Wang
Polyamidoamine modified organo-montmorillonites (OMMT) bearing flame-retardant elements were prepared through montmorillonite (MMT) and polyamidoamines with different generations. These materials turned out to be a possible use for the flame-retardency of polymers. Different generations of dendrimers, G1.0, G2.0, and G3.0, were synthesized from a cyclotriphosphazene core. Characteristics of these dendrimers were studied by Fourier transform infrared (FTIR) spectroscopy, hydrogen nuclear magnetic resonance ( 1 H-NMR), ultraviolet (UV-Vis) spectroscopy, and intrinsic viscosity (IV). It was demonstrated that different generations of dendrimers had been well synthesized. These dendrimers were used as an organic intercalation agent to modify natural clay, Na + -MMT. Different generations of flame-retardant dendrimer modified OMMT, G1.0-OMMT, G2.0-OMMT, and G3.0-OMMT, were prepared. The interlayer spacing, thermal stability, and surface morphology of these dendritic MMTs were investigated by FTIR, thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM) as well as energy dispersing spectrometry (EDS). It was seen that the thermal stability of G1.0-OMMT, G2.0-OMMT, and G3.0-OMMT was obviously improved with the increase of generations compared with that of MMT. In addition, an exfoliated structure existed in these dendrimer modified silicate layers.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Guowei Li,Siming Yu,Wei Xue,Dong Ma,Wu Zhang
Fabrication of nitric oxide (NO) donors with good biocompatibility and high NO loading efficiency for antibacterial applications remains a big challenge. In the present work, using low molecular weight chitosan (CS) with high water solubility as the backbone, we designed and synthesized a poly(amidoamine) (PAMAM) dendron-grafted CS using a fast and simple copper-catalyzed azide-alkyne cyclization reaction of azide-modified CS (CS-N 3 ) and propargyl focal point PAMAM dendrons with the third generation. NO was loaded on CS-PAMAM via a reaction with its secondary amine groups, which resulted in the formation of CS-PAMAM/NONOate with a maximal NO loading amount of 1.7 μmoL/mg. The CS-PAMAM/NONOate showed significant antibacterial activity against both Gram-negative E. coli and Gram-positive S. aureus , where 2.5 mg of CS-PAMAM/NONOate showed an inhibition zone with a diameter of about 20 mm. Moreover, the CS-PAMAM/NONOate presented negligible toxicity on normal mouse embryonic fibroblast cells, suggesting excellent biocompatibility. These findings indicate that the prepared CS-PAMAM/NONOate is promising for use in wound dressing industry as wound healing and treatment of anti-bacterial infections.
研究领域:
材料科学
·
生物学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 5
·124800
·
IF 7.5
Yunqi Chen,Xiang Wang,Haoyuan Sun,Xinqiang Wang,Baihu Dong,Chaogang Fan,Yingfeng Tu,Shaohui Lin,Qinmin Pan,Xianshe Feng,Flora T.T. Ng
Efficient separation of surfactant-stabilized oil-water emulsions remains a significant challenge. Janus membranes, featuring distinct chemical properties on opposite surfaces, have attracted considerable attention for emulsion separation applications. In this work, we report the fabrication of a Janus fiber membrane with a highly hydrophilic/underwater oleophobic polyethylene terephthalate (PET) side and a hydrophobic/underwater superoleophilic polyvinylidene fluoride (PVDF) side via electrospinning and chemical modification. Hydrophilic polyamidoamine (PAMAM) dendrimers were successfully grafted onto the PET side to achieve a wettability transformation. For the representative n-hexane systems, the PET-G3-facing configuration gave a permeance of 975 L⋅m −2 ⋅h −1 ·bar −1 and a separation efficiency of 96.9% for Hex/W emulsion, whereas the PVDF-facing configuration gave a permeance of 746 L⋅m −2 ⋅h −1 ·bar −1 and a separation efficiency of 99.5% for W/Hex emulsion. Also, the membrane achieved > 96.0% efficiency for Chl/W, Tol/W, Hex/W, and Hep/W emulsions, 91.6% for the Die/W emulsion, and > 99.0% for W/Hex, W/Hep, W/Tol, and W/Chl emulsions. Furthermore, the separation efficiencies remained above 96.9% and 98.0% after 10 continuous separation cycles for O/W (oil-in-water) and W/O (water-in-oil) emulsions. Additionally, after bovine serum albumin (BSA) protein contamination and simple water washing, the flux recovery rate (FRR) of this Janus membrane maintained 90.3%, demonstrating its strong anti-fouling properties. In summary, this study presents a sustainable and effective strategy for preparing Janus membranes using PET as a raw material, offering valuable insights into the separation of oil-water emulsions.
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产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 8
·期 2
·1140-1146
·
IF 7.394
Chunmei Gao,Shaoyu Lü,Mingzhu Liu,Can Wu,Yun Xiong
The synthesis and properties of CO2 responsive and fluorescent dendritic polymers, poly(amido amine)/Pluronic F127 (PAMAM/F127), are reported in this paper. The morphologies and sizes of PAMAM/F127 dendritic polymers were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). PAMAM/F127 dendritic polymers showed unimolecular micelle morphologies at low concentrations, and changed to multimolecular micelles at higher concentrations. Additionally, fluorescence spectra and confocal laser scanning microscopy images showed that PAMAM/F127 dendritic polymers exhibited a fluorescent enhancement response to the presence of CO2. Apart from that, the release behavior of PAMAM/F127 gels under simulated body fluids was investigated by choosing curcumin as the hydrophobic drug. The results indicated that PAMAM/F127 dendritic polymers can be used to improve the solubility of curcumin, and the drug released faster in the presence of CO2. Such CO2 responsive fluorescent dendritic polymers are potentially applicable in cellular imaging or drug controlled release.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2022
·Vol. 32
·期 3
·840-853
·
IF 3.518
Cui Can,Xie Ya-Dian,Niu Jiao-Jiao,Hu Hai-Liang,Lin Sen
In this work, three different generations of Poly(amidoamine) dendrimers (PAMAM) decorated on magnetic Fe 3 O 4 composites (Fe 3 O 4 @SiO 2 –G1, Fe 3 O 4 @SiO 2 –G3, Fe 3 O 4 @SiO 2 –G5) were fabricated and characterized by FTIR, XRD, TEM, TGA, VSM and XPS. The obtained composites were used for Cr(VI) removal. Batch adsorption studies showed that the adsorption reached equilibrium within 60 min, and the optimal pH was 3.0. The result of adsorption kinetics was simulated by the pseudo-second-order model. The adsorption equilibrium isotherm was well fitted with the Langmuir adsorption model. Furthermore, thermodynamics calculations revealed that the adsorption process was endothermic and spontaneous. Importantly, adsorption capacity of Cr(VI) obeyed the sequence of Fe 3 O 4 @SiO 2 –G1 < Fe 3 O 4 @SiO 2 –G5 < Fe 3 O 4 @SiO 2 –G3, 3 generation of PAMAM (G3) was the optimal for adsorption capacity of Cr(VI). The maximum theoretical Cr(VI) adsorption capacity ( q m ) of Fe 3 O 4 @SiO 2 –G3 was 334.45 mg/g, and removal ration remained above 89.5% after five cycles of adsorption–desorption. Thus, Fe 3 O 4 @SiO 2 –G3 is predicted to be an efficient adsorbent for the adsorption of Cr(VI) from aqueous solution, and the obtained results can help in the generation optimization during fabrication of dendrimer modified adsorbents.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Minjun Ma,Yu Liu,Honghong Chang,Yingxiong Wang,Yan Qiao
Ofloxacin is one of the most potent antibiotics. But its low solubility in water has limited its use in site targeted delivery and topical applications. This study investigates the PAMAM-NH 2 (amine-terminated polyamidoamine dendrimers) delivery of ofloxacin in water based on host-guest chemistry and reveals the interaction mechanism of PAMAM-NH 2 with ofloxacin by utilizing NMR techniques including 1D chemical shift titration, 2D 1 H– 13 C HSQC titration, NOESY technique, STDD method, and 2D DOSY analysis. Ofloxacin interacts with surface amine groups of PAMAM-NH 2 via ionic interactions, and ofloxacin is also encapsulated into the PAMAM interior cave through hydrophobic interactions. An overall mechanism was proposed for the PAMAM-NH 2 adsorption of ofloxacin, and then the binding affinity was determined. The experimental data demonstrate the potential of the PAMAM-NH 2 for the drug molecules delivery.
研究领域:
药学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF GENE MEDICINE
·
2020
·Vol. 22
·期 10
·e3240
·
IF 3.258
Dongli Wang,Jing Wang,Jie Song,Qing Shen,Ruifeng Wang,Weiyue Lu,Jun Pan,Cao Xie,Min Liu
Background Gene therapy has become a potential strategy for cancer treatment. However, the development of efficient gene vectors restricts the application for cancer gene treatment. Functionalization of polymers with functional groups can significantly improve their transfection efficacy. Methods Guanidyl can form bidentate hydrogen with the phosphate groups and phosphate groups are present in DNA and cell membranes, thus increasing DNA condensation and cellular uptake. Imidazolyl has high buffering capacity in endosomal/lysosomal acidic environment, facilitating endosome/lysosome escape. We designed a structure-integrated group of guanidyl and imidazolyl, 2-aminoimidazole (AM), which was conjugated to PAMAM generation 2 (G2) for gene therapy of gastric adenocarcinoma. Results Molecular docking results illustrated that G2-AM bound with DNA molecule effectively via multiple interactions. A quantitative luciferase assay showed that the transfection efficacy of G2-AM/pGL3 was approximately 100-fold greater than that of G2/pGL3, 90-fold greater than that of imidazolyl-modified G2 (G2-M) /pGL3 and 100-fold greater than that of G5/pGL3 without additional cytotoxicity. After introducing the pTRAIL gene into gastric adenocarcinoma cells, the apoptosis ratio of gastric adenocarcinoma cells treated with G2-AM/pTRAIL was 36.95%, which is much larger than the corresponding ratio of G2/pTRAIL (7.45%), G2-M/pTRAIL (11.33%) and G5/pTRAIL (23.2%). In a gastric adenocarcinoma xenograft model, the in vivo transfection efficacy of G2-AM/pRFP was much greater than that of G2/pRFP and G2-M/pRFP. Conclusions These results demonstrate that AM could be modified with cationic polymers for potential application in gene delivery and gastric adenocarcinoma gene therapy.
研究领域:
癌症治疗
·
分子生物学
·
聚合物化学
·
基因递送
✓ 已验证
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2026
·
IF 5.9
Huaiying Qin,Yefan Wang,Xin Li,Xiaoyun Zhang,Guanghui Zhao
Abstract Existing research of single polymer systems has primarily focused on the modification and optimization, with limited systematic comparisons of the performance evolution and gene transfection differences between branched polyethyleneimine (bPEI) and dendritic polyamide-amine (PAMAM) after fluorination modification. In this study, a series of fluorinated polymers were prepared by fluorinating 25 kDa bPEI and PAMAM G4 using heptafluorobutyric anhydride. The pEGFP plasmid was employed as a model gene. The gene transfection efficiencies of both types of materials before and after fluorination were systematically compared and evaluated using fluorescence analysis, flow cytometry, and in vivo imaging techniques. The results demonstrated that fluorination significantly enhanced transfection efficiency under serum-containing conditions. PAMAM G4-F773 in particular exhibited superior performance compared to fluorinated bPEI and the commercial Lipofectamine 2000 transfection reagent. This study represents the first systematic comparison of transfection performance between these two types of fluorinated polymers, providing crucial insights for rational design of gene delivery vectors.
✓ 已验证
DOI
高影响因子
ENERGY & FUELS
·
2014
·
IF 2.733
Xing Yao,Bin Jiang,Luhong Zhang,Yongli Sun,Xiaoming Xiao,Zhiheng Zhang,Zongxian Zhao
Waste water resulted from polymer flooding oil recovery generally has a bad impact on the subsequent process of enhanced oil recovery. Separating residual oil from oil/water (O/W) emulsion with suitable kinds of demulsifier is one strategy generally adopted by oil companies. Because of the existence of large amounts of ultrafine oil droplets with the average diameter less than 2 μm, the emulsions can be extremely difficult to break up. To solve this problem, an amine-based dendrimer demulsifier PAMAM (polyamidoamine) was synthesized in this study, and the efficiency of the demulsifier in dealing with O/W emulsions with ultrafine oil droplets was investigated. Because of its strong interfacial activity and relatively good solubility in water, the dendrimer-based demulsifier can easily attach to emulsified oil droplets in a stable diesel-in-water emulsion. The influences of temperature, settling time, and concentration of the demulsifier used on the efficiency of the demulsifier were investigated in detail. The optimal operating condition under which more than 90% oil was removed from the original emulsion by the demulsifier was found. In contrast, less than 2% oil was removed from the emulsion without applying the demulsifier under the same conditions. Micrographs showed that the PAMAM demulsifier could lead to the breakup of diesel-in-water emulsions with ultrafine oil droplets by flocculation and coalescence. The surface tension and interfacial tension at the diesel–water interface were also measured to give a basic understanding of the demulsification mechanism. Though not perfect in dealing with emulsions with the average oil droplets less than 2 μm due to the relatively high demulsifier dosage, its relatively simple synthetic procedure and mild operating conditions showed a great promise in industrial applications with unique advantages over traditional physical methods.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）