INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Yongjing Xu,Bo Liu,Liming Zou,Chenkai Sun,Wengang Li
In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method. PLLA and dichloromethane/ethanol (CH 2 Cl 2 /EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning. The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL. The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA. The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°–19°, 109%–482%, 1945–2517 g m −2 day −1 and 4–14%, respectively. The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA. The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats. Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.
研究领域:
生物材料学
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组织工程学
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医学材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER TESTING
·
2022
·
IF 4.931
Yuezhou Liu,Yanjing Liu,Xiyue Li,Yongfang Qian,Lihua Lv,Ying Wang
In order to fabricate antibacterial nanofiber membranes for tissue engineering field, the oxidative damage of magnesium hydroxide nanoparticles (Mg(OH) 2 NPs) against the model system of Escherichia coli ( E. coli ) was researched by measuring the oxidative stress response, oxidative stress products and glucose metabolism. The toxicity of Mg(OH) 2 NPs to a normal biological system was evaluated in vivo through the acute oral toxicity, blood physiological and serum biochemical markers and acute skin irritation, indicating that the Mg(OH) 2 NPs exhibited great biosafety. The Mg(OH) 2 NPs with antibacterial property and biosafety were loaded on PCL/PVP nanofiber substrates through electrospinning technology to fabricate the Mg(OH) 2 /PCL/PVP nanofiber membranes. The results of characterization and antibacterial test showed that the Mg(OH) 2 /PCL/PVP nanofiber membranes with 8.0 wt% Mg(OH) 2 NPs present excellent antibacterial rate of 100% against E. coli that the Mg(OH) 2 NPs can be physically and evenly loaded on nanofiber substrates.
研究领域:
材料科学
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纳米技术
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组织工程
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2016
·
IF 2.641
Xiaodong Zhu,Shilei Ni,Tongliang Xia,Qingyu Yao,Haoyuan Li,Benlin Wang,Jiangang Wang,Xingang Li,Wandong Su
ABSTRACT: Electrospun poly(ε-caprolactone) (PCL)/gelatin (GT) scaffolds were developed to provide controlled release of 7-ethyl-10-hydroxy camptothecin (SN-38). Acetic acid was introduced to improve the miscibility of PCL and GT to produce a homogeneous nanofiber membrane mixture. The effect of SN-38 content in binary mixtures on processability, fiber morphology, water sorption, swelling, and drug release was investigated. Electrospun PCL/GT blend nonwoven fibers showed fiber surface roughness, decreased PCL crystallinity, and increased swelling with increasing drug content of 1, 2, and 4 wt %. Additionally, increasing the SN-38 concentration reduced the degradation rate of the GT. Furthermore, we hypothesize the existence of a drug content saturation point in the monoaxial fiber to explain the different drug release patterns of PG2 compared with those of PG1 and PG4. The matrix also showed good biodegradation and anti-tumor function. Our results demonstrate that SN-38-loaded PCL/GT fibers can be obtained by electrospinning. The SN-38-loaded fibers merit further evaluation as a means to potentially prevent locoregional recurrence following surgical tumor resection.
研究领域:
生物医学工程
·
肿瘤学
·
药剂学
✓ 已验证
DOI
高影响因子
Biomedical Materials
·
2022
·Vol. 17
·期 5
·055008
·
IF 4.103
Zini Liu,Xueqi Chen,Chen Li
Asymmetrical dressings, which are composed of a compact top layer and a porous bottom layer, are commonly used to mimic the characteristics and structure of the epidermis and dermis layers, and overcome the flaws of traditional dressings such as wound dryness and bacterial penetration. Herein, a bio-inspired double-layer asymmetric wettable wound dressing was prepared by low-temperature 3D printing coupled with electrospinning technology. The hydrophobic top layer of poly(caprolactone)(PCL) film produced by electrospinning was used to simulate the compact and air-permeable epidermis. The hydrophilic bottom layer of the dressing, a scaffold composed of chitosan and copper ions doped Laponite (Cu@CS-Lap) was used to kill bacteria and speed up wound healing. Additionally, the composite dressings also showed excellent cytocompatibility and antibacterial properties in vitro experiments. The migratory area of Cu-doped group human umbilical vein endothelial cells increased by about 48.19% compared to the control group, as revealed by the results of the cell scratch experiment. Furthermore, in vivo experiments in rats showed that wound closure at the 0.5Cu@CS5-PCL dressing reached 98.24% after 12 days, indicating the enormous potential of asymmetric double dressings in boosting wound healing.
研究领域:
生物材料
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组织工程
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伤口愈合
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Siyu Wu,Jiayi Ma,Jun Liu,Chun Liu,Su Ni,Ting Dai,Yan Wang,Yiping Weng,Hongbin Zhao,Dong Zhou,Xiubo Zhao
Biomaterial–immune system interactions play an important role in postimplantation osseointegration to retain the functionality of healthy and intact bones. Therefore, appropriate osteoimmunomodulation of implants has been considered and validated as an efficient strategy to alleviate inflammation and enhance new bone formation. Here, we fabricated a nanostructured PCL/PVP (polycaprolactone/polyvinylpyrrolidone) electrospinning scaffold for cell adhesion, tissue ingrowth, and bone defect padding. In addition, telmisartan, an angiotensin 2 receptor blocker with distinct immune bioactivity, was doped into PCL-/PVP-electrospun scaffolds at different proportions [1% (TPP-1), 5% (TPP-5), and 10% (TPP-10)] to investigate its immunomodulatory effects and osteoinductivity/conductivity. Telmisartan-loaded scaffolds displayed in vitro anti-inflammatory bioactivity on lipopolysaccharide-induced M1 macrophages by polarizing them to an M2-like phenotype and exhibited pro-osteogenic properties on bone marrow-derived mesenchymal stem cells (BMSCs). Histological analysis and micro-CT results of a rat skull defect model also showed that the telmisartan-loaded scaffolds induced a higher M2/M1 ratio, less inflammatory infiltration, and better bone regenerative patterns. Furthermore, activation of the BMP2 (bone morphogenetic protein-2)-Smad signaling pathway was found to be dominant in telmisartan-loaded scaffold-mediated macrophage–BMSC interactions. These findings indicate that telmisartan incorporation with PCL/PVP nanofibrous scaffolds is a potential therapeutic strategy for promoting bone healing by modulating M1 macrophages to a more M2 phenotype at early stages of postimplantation.
研究领域:
生物材料
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免疫学
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组织工程
·
再生医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2019
·
IF 3.78
Qun Liu,Haipeng Wang,Lei Chen,Wulong Li,Yakun Zong,Yi Sun,Zhanxiong Li
Fluorinated Poly(ε-caprolactone) (PCL) block copolymers PCL-PTFOA(2 h), PCL-PTFOA(4 h), PCL-PTFOA(6 h), PCL-PTFOA(8 h) were prepared via esterification by using poly(1H,1H,2H,2H-perfluorooctylacrylate) (PTFOA) with different molecular weights as reactants. It was found that PCL-PTFOA(6 h) and PCL-PTFOA(8 h) possessed the similar molecular weight via GPC analysis. PCL and fluorinated PCL block copolymers films were prepared by solution casting method. The structure and properties of film surface of the fluorinated PCL block copolymers were analyzed by energy dispersive X-ray spectrometer (EDS), scanning electron microscope (SEM), Differential scanning calorimetry (DSC), water contact angles (WCAs) and the surface free energy. The results showed that the fluorinated PCL films possessed improved hydrophobicity because of the microphase separation between fluoropolymer chain and PCL chain. The enzymatic degradation of PCL, PCL-PTFOA(2 h), PCL-PTFOA(4 h) and PCL-PTFOA(6 h) films were performed at 37 °C in a phosphate buffer solution (PBS, 0.1 M, with a pH of 7.2–7.4) with lipase and analyzed by weight loss, SEM and EDS . It was found that the introduction of fluorine-containing segment reduced the rate of enzymatic degradation of the copolymer film in the early period, while increased the finally weight loss rate with a prolonged time. In addition, the enzymatic degradation rate of PCL-PTFOA(2 h) film and PCL-PTFOA(4 h) film was faster than that of PCL-PTFOA(6 h) film.
研究领域:
高分子科学
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材料科学
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生物降解
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表面化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2016
·
IF 4.32
Yunzhu Qian,Hanbang Chen,Yang Xu,Jianxin Yang,Xuefeng Zhou,Feimin Zhang,Ning Gu
Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.
研究领域:
生物材料
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组织工程
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细胞生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Lei Yang,Zhenghai Zhang,Jian Hou,Xin Jin,Zhongcheng Ke,Dan Liu,Mei Du,Xiaobing Jia,Huixia Lv
Ginsenoside compound K (CK) is one of the effective ingredients in antitumor composition of ginsenoside. However, the poor water solubility and significant efflux have limited the widespread clinical use of CK. In this study, preparation of novel CK-loaded d -alpha-tocopheryl polyethylene glycol 1,000 succinate/poly(ethylene glycol)-poly(ε-caprolactone) mixed micelles (CK-M) is discussed to solve the above problems. Particle size, zeta potential, and morphology were characterized using dynamic light scattering and transmission electron microscopy. CK-M are spherical shaped with an average particle size of 53.07±1.31 nm with high drug loading of 11.19%±0.87% and entrapment efficiency of 94.60%±1.45%. Water solubility of CK was improved to 3.78±0.09 mg/mL, which was ~107.35 times higher than free CK. A549 and PC-9 cells were used to evaluate in vitro cytotoxicity and cellular uptake. IC 50 values of CK-M in A549 and PC-9 cells (24 h) were 25.43±2.18 and 18.35±1.90 μg/mL, respectively. Enhanced cellular uptake of CK-M was observed in both cells. Moreover, CK-M promoted tumor cell apoptosis, inhibited tumor cell invasion, metastasis, and efflux through regulation of Bax, Bcl-2, matrix metalloproteinase-2, Caspase-3, and P-glycoprotein. In vivo imaging indicated that CK-M has excellent tumor targeting effect within 24 h, and the relative tumor inhibition rate of CK-M was 52.04%±4.62% compared with control group ( P <0.01). Thus, CK-M could be an appropriate delivery agent for enhanced solubility and antitumor effect of CK.
研究领域:
药学
·
肿瘤学
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纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2023
·Vol. 63
·期 7
·2084-2092
·
IF 3.2
Gaoyuan Ye,Tongfei Gu,Boyuan Chen,Hongjie Bi,Yingcheng Hu
To improve the toughness of polylactic acid (PLA), in this research, a tough polymer polycaprolactone (PCL) was combined with PLA to prepare blends, and a compatibilizer of glycidyl methacrylate grafted PLA (PLA-g-GMA) was produced to enhance the compatibility of PLA/PCL blend. Compared with PLA/PCL blend, the compatibilized blend exhibited a lower melting temperature and greater thermal stability. The tensile test indicates that the addition of compatibilizer greatly improved the toughness of the PLA/PCL blend. Particularly, the elongation at break of PLA/PCL blend with 6 wt% PLA-g-GMA increased to 370% and reached about 66 times of neat PLA. In addition, compatibilized blends have good shape memory performance. The research provides a simple and feasible method to overcome the brittleness of PLA, supplying a new strategy to modify PLA.
研究领域:
材料科学
·
高分子科学
·
工程学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Jupei Zhang,Hangbin Xia,Xuerui Zhou,Zhaoxu Meng,Qishu Jin,Dongmin Chen,Xiaojuan Xia,Yiren Jiao,Jiang Chang,Zhihong Dong,Zhen Zeng,Hongshi Ma,Chen Yang
Breast reconstruction after mastectomy aims to restore breast appearance and function. Current scaffolds often fail to meet the complex clinical demands of post-mastectomy breast reconstruction, which include personalized shaping, prevention of tumor recurrence and metastasis, and promotion of adipose tissue regeneration. This study aims to address these challenges by combining a readily processable biopolymer, polycaprolactone (PCL), with a multifunctional bioactive ceramic, cobalt orthosilicate (Co 2 SiO 4 , CoSi) to form a multi-functional CoSi/PCL composite scaffold through 3D printing technology. The scaffold exhibits a controllable shape with designed macroporous architecture. Its photothermal performances are well presented in both near-infrared (NIR) I and NIR-II regions, enabling effective tumor ablation with minimal side effects. Additionally, the release of bioactive silicate and cobalt ions promotes adipogenesis and angiogenesis, thereby enhancing the integration of the scaffold with surrounding tissues. This approach offers a promising strategy for combining tumor therapy with breast reconstruction, providing a clinically relevant solution for post-mastectomy patients.
研究领域:
生物医学工程
·
组织工程
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Mianji Qiu,Zhi Geng,Wangjian Cheng,Chengyuan Hua,Xing Yan,Baoyun Ye,Chongwei An,Jingyu Wang
Solid propellants are essential for rocket engine efficiency and safety. The introduction of energetic self-healing adhesives can improve its energy density, mechanical properties, and safety, while extending its service life. Therefore, this paper proposes a novel energetic self-healing adhesive that utilizes asymmetric alicyclic isophorone diisocyanate (IPDI) and 2-(aminophenyl) disulfide (2-AFD), which has a bent biphenyl ring structure, as hard segments, combined with semicrystalline polymer polycaprolactone (PCL) and energetic adhesive glycidyl azide polymer (GAP) as soft segments to synthesize a series of self-healing adhesives. By adjusting the hard segment content, a balance between mechanical properties and self-healing performance is achieved. Results show that increasing the hard segment content enhances toughness (from 26.60 MJ·m −3 to 58.54 MJ·m −3 ), but decreases self-healing efficiency (from 90 % to 58 %). GPPU-2 exhibits 38.95 MJ·m −3 toughness and recovers 86 % of its toughness within 90 min at 80 °C via dynamic disulfide and hydrogen bond interactions. The GPPU-2-based propellant, GPPU80, recovers 88.22 % of tensile strength after 24 h at 80 °C, demonstrating effective crack propagation inhibition. Compared to inert adhesive-based propellants, GPPU80 shows superior combustion performance and energy release. This study offers insights for designing high-performance self-healing adhesives to enhance propellant safety and energy density.
研究领域:
材料科学
·
航天工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Biological Engineering
·
2024
·Vol. 18
·期 1
·1-14
·
IF 5.7
Wang Chenglong,Dong Jinlei,Liu Fanxiao,Liu Nan,Li Lianxin
Background The long-term nonunion of bone defects is always a difficult problem in orthopaedics treatment. Artificial bone implants made of polymeric materials are expected to solve this problem due to their suitable degradation rate and good biocompatibility. However, the lack of mechanical strength, low osteogenic induction ability and poor hydrophilicity of these synthetic polymeric materials limit their large-scale clinical application.
Results In this study, we used bioactive glass (BG) (20%, W/W) and polycaprolactone (PCL, 80%, W/W) as raw materials to prepare a bone repair scaffold (PCL@BG20) using fused deposition modelling (FDM) three-dimensional (3D) printing technology. Subsequently, stromal cell-derived factor-1α (SDF-1α) chemokines were loaded into the PCL@BG20 scaffold pores with gelatine methacryloyl (GelMA) hydrogel. The experimental results showed that the prepared scaffold had a porous biomimetic structure mimicking that of cancellous bone, and the compressive strength (44.89 ± 3.45 MPa) of the scaffold was similar to that of cancellous bone. Transwell experiments showed that scaffolds loaded with SDF-1α could promote the recruitment of bone marrow stromal cells (BMSCs). In vivo data showed that treatment with scaffolds containing SDF-1α and BG (PCL@BG-GelMA/SDF-1α) had the best effect on bone defect repair compared to the other groups, with a large amount of new bone and mature collagen forming at the bone defect site. No significant organ toxicity or inflammatory reactions were observed in any of the experimental groups.
Conclusions The results show that this kind of scaffold containing BG and SDF-1α serves the dual functions of recruiting stem cell migration in vivo and promoting bone repair in situ. We envision that this scaffold may become a new strategy for the clinical treatment of bone defects.
研究领域:
骨科
·
组织工程
·
生物材料
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）