TALANTA
·
2017
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IF 4.162
Yamin Fu,Lu Wang,Yinghao Duan,Lina Zou,Baoxian Ye
A one-step and facile method using SnCl 2 ·H 2 O as reducing agent to reduce graphene oxide (GO) was performed in the aid of poly(diallyldimethylammonium chloride) solution (PDDA). SnCl 2 ·H 2 O is not only a reducing agent for graphene oxide (GO), but also a precursor of SnO 2 . SnO 2 -PDDA-GR composite was characterized by various surface, structural and electrochemical analysis techniques, such as transmission electron microscopy (TEM), UV spectrum (UV–vis), Infrared Spectrum (IR), X-ray diffraction (XRD), Cyclic voltammograms (CV) and electrochemical impedance (EIS). The SnO 2 -PDDA-GR composite was used to constructed electrochemical sensor (SnO 2 -PDDA-GR/GCE) for the determination of daidzein . Under the optimized experimental condition, it was found that the response of peak current is linear to the concentration of daidzein in the ranges of 2.0×10 −8 −1.0×10 −6 mol L −1 , and the detection limit was estimated to be 6.7×10 −9 mol L −1 (S/N=3). Furthermore, this sensor was successfully applied for the determination of daidzein in traditional Chinese medicine (pueraria lobata) and Daidzein tablets.
研究领域:
材料科学
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电化学
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分析化学
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药物分析
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yuanyuan Liu,Bangyan Liu,Lian Xia,Hongyan Yu,Qiaodie Wang,Yuangen Wu
Although methods based on mass spectrometry or immunoassays can meet the demands of paraquat detection, their application is limited by a lack of convenience or stability. Herein, a facile visual aptamer-based chromatographic strip (CS) was fabricated for the rapid paraquat detection using the cationic polyelectrolyte poly(diallyl dimethyl ammonium chloride) (PDDA) as a powerful capture molecule at test-line (T-line). The PDDA could capture the gold nanoparticles (AuNPs) through strong electrostatic interactions for visual detection. The proposed CS showed an excellent response for paraquat in the range of 20–150 μg·L −1 , and the limit of detection (LOD) was as low as 4.28 μg·L −1 . This detection method has good stability and specific selectivity , and can visually determine paraquat residues more than 30 μg·L −1 by naked eye within 3 min. The results demonstrated that the prepared CS based on aptamer and cationic polyelectrolyte is an accurate and practicable tool for rapid analysis of pesticide residues .
研究领域:
分析化学
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环境科学
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农业科学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 142
·期 5
·e56430
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IF 2.7
Chuntao Zhu,Meng Yu,Zijian Liang,Lanhe Zhang,Jian Zhang
This paper reports the formation of coacervates by the electrostatic interaction of poly (diallyldimethylammonium chloride) (PDDA) and adenosine triphosphate (ATP) in aqueous solution, examining its formation conditions, stability, and efficiency in separation. The ideal concentration for creating coacervate droplets in pure water, HEPES buffer, and NaCl solution was determined to be 20 mM of PDDA and ATP. Enhancing the stability of coacervates was achieved by incorporating phospholipid vesicles on their surface, presenting a novel strategy for building cell models. Ostwald Ripening was employed to comprehend the growth mechanism of the coacervates, while the Hofmeister Ion Series and Schulze-Hardy's rule were utilized to elucidate the stability differences in solutions containing NaCl, Na 2 SO 4 , and MgCl 2 . These coacervates were stable at concentrations below 90 mM NaCl, 200 mM Na 2 SO 4 , and 30 mM MgCl 2 , respectively. We also explored the specific separation of dyes and tetracycline hydrochloride (TC) in the coacervates. Separation efficiencies of 92.98% for methylene blue (MB), 94.19% for methyl orange (MO), and 85.94% for TC, were achieved by the coacervates, which can be attributed to the synergistic effects of hydrophobicity, electrostatic forces, and π-π interactions. The proposed coacervates have great potential in cell mimicry and water treatment.
研究领域:
材料科学
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化学工程
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生物物理
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环境科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Danya Lyu,Jing Li,Xiaowen Wang,Weiwei Guo,Erkang Wang
DNA-templated silver nanoclusters (DNA–Ag NCs) have shown great potential in various bioanalysis and bioimaging applications, owing to their facile synthesis and ultrasmall sizes and especially their programmable fluorescence emission depending on the sequences of DNA templates. However, the bioimaging applications of DNA–Ag NCs are severely limited by their poor stability in physiological environments and their poor cell permeability, resulting from the highly negatively charged DNA backbones. In this paper, cationic polyelectrolytes were used to modify fluorescent DNA–Ag NCs via electrostatic interactions between the positive polymer backbones and the negatively charged phosphate groups of the DNA strands. The fluorescence properties of a typical single-stranded-DNA (ssDNA)-templated Ag NC changed dramatically after incorporation with the cationic polyelectrolyte caused by conformational changes in the DNA, with a 3-fold fluorescence-emission enhancement of the Ag NCs observed after incorporation with a typical cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA), whereas the fluorescence of double-stranded-DNA (dsDNA)-templated Ag NCs changed little after incorporation. Moreover, the modification with PDDA greatly prolonged the stability of both ssDNA- and dsDNA-stabilized Ag NCs against nuclease digestion and enhanced the cell uptake of the DNA–Ag NCs. Rapid cell imaging was demonstrated using NIH/3T3 cells as a model system.
研究领域:
纳米技术
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生物化学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 23
·9633-9645
·
IF 4.5
Hu Rongqin,Mu Zhaode,Gong Feng,Qing Min,Yuan Yonghua,Bai Lijuan
Human epididymis protein 4 (HE4) is a highly sensitive and specific biomarker for diagnosing ovarian cancer. In this work, a new electrochemical aptasensor for rapid and sensitive detection of HE4 was constructed. Firstly, metal–organic framework/ketjen black (NH 2 -MIL-53(Al)/KB) composite with abundant amino groups and large specific surface area was prepared as signal amplifier to anchor gold nanoparticles (AuNPs). Subsequently, signal probe (SP) and a large amount of electroactive toluidine blue (Tb) were attached on AuNPs to form the tracer label, producing a distinct electrochemical detection signal. Moreover, Ni 3 (HITP) 2 was modified by poly(diallyldimethylammonium chloride, PDDA) to obtain PDDA/Ni 3 (HITP) 2 , and then it was covered with deposited gold nanocrystals (DpAu) to form the sensing platform, which increased the electrical conductivity, specific surface area and the loading of triple-helix assembled probes (TAP). Target HE4 was bound to its aptamer in the outer layer of the TAP, leaving a separate capture probe (CP) on the electrode surface that can bind with the tracer label. The proposed aptasensor for HE4 detection showed a wide linear range from 1 to 10 nM with a detection limit of 0.41 fM. In addition, the aptasensor has good selectivity, stability and reproducibility. More importantly, this method showed satisfactory results in clinical serum sample analysis compared with ELISA, possessing application prospects in ovarian cancer diagnosis.
研究领域:
生物传感器
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分析化学
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纳米技术
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癌症诊断
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电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）