TALANTA
·
2023
·
IF 6.1
Jun Xu,Ying Wang,Menghua Huang,Xiaojuan Xu,Yueyun Zeng,Xuan Luo,Shicheng Pei,Keming Xu,Wenying Zhong
Compared to conventionally synthesized organic compounds, peptides with amphiphiles have unique advantages, especially in self-assembly. Herein, we reported a peptide-based molecule rationally designed for the visual detection of copper ions (Cu 2+ ) in multiple modes. The peptide exhibited excellent stability, high luminescence efficiency, and environmentally responsive molecular self-assembly in water. In the presence of Cu 2+ , the peptide undergoes an ionic coordination interaction and a coordination-driven self-assembly process that leads to the quenching of fluorescence and the formation of aggregates. Therefore, the concentration of Cu 2+ can be determined by the residual fluorescence intensity and the color difference between peptide and competing chromogenic agents before and after Cu 2+ incorporation. More importantly, this variation in fluorescence and color can be presented visually, thus allowing qualitative and quantitative analysis of Cu 2+ based on the naked eye and smartphones. Overall, our study not only extends the application of self-assembling peptides but also provides a universal method for dual-mode visual detection of Cu 2+ , which would significantly promote point-of-care testing (POCT) of metal ions in pharmaceuticals, food, and drinking water .
研究领域:
分析化学
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材料科学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xiaoqing Lv,Zhengtai Ma,Jiaqi Xu,Zhen Wang,Guixian Zhu,Beiju Huang
The paper-based analytical platforms have been applied in biochemical detection and environmental monitoring due to their low cost and easy operation. Despite their advantages, the complexity of fabrication has limited their further application. Here, a simple and innovative approach using parylene C, a material commonly used in semiconductors, is reported to fabricate paper-based chips and construct a point-of-care testing (POCT) platform through smartphones. Parylene C is deposited on the filter paper through a polydimethylsiloxane (PDMS) clamp and forms a hydrophobic barrier. A three-channel paper-based chip was utilized to detect aluminum (Al) ions on a smartphone platform by the chromogenic method. The color change after Al 3+ detection is represented with △E which was photographed and calculated by app on smartphone. The detection limit is 0.05 mg/L and the detection range is from 0.05 mg/L to 10 mg/L which indicates the application potential in the field of biochemical detection. This new paper-based sensing method and smartphone-based portable detection platform are simple, low-cost, fast, and highly sensitive, and provides a potential application for on-site, visual detection of ions and even other substances.
研究领域:
分析化学
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环境科学
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即时检测
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智能手机应用
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材料科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-9
·
IF 5.3
Liu Shendong,Chen Xinlian,Zhao Hong,Lin Tianran,Hou Li,Zhao Shulin
An ultrasensitive photothermal assay was designed for point-of-care testing (POCT) of tumor markers based on a filter membrane. Firstly, Cu 2-x Se was successfully encapsulated in liposome spheres with biotin on the surface and connected to carcinoembryonic antigen (CEA) aptamer with 3'end modified biotin by streptavidin. Secondly, the CEA antibody was successfully modified on the surface of the nitrocellulose membrane through simple incubation. Finally, the assay process was completed using a disposable syringe, and the temperature was recorded using a handheld infrared temperature detector. In the range 0–50 ng mL −1 , the temperature change of the nitrocellulose membrane has a strong linear relationship with CEA concentration, and the detection limit is 0.097 ng mL −1 . It is worth noting that the entire testing process can be easily performed in 10 min, much shorter than traditional clinical methods. In addition, this method was successfully applied to the quantitative determination of CEA levels in human serum samples with a recovery of 96.2–103.3%. This rapid assay can be performed by “one suction and one push” through a disposable syringe, which is simple to operate, and the excellent sensitivity reveals the great potential of the proposed strategy in the POCT of tumor biomarkers. Graphical Abstract
研究领域:
生物医学工程
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分析化学
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即时诊断
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癌症生物标志物
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yan Liu,Yuetao Zhang,Zijie Li,Zhiqi Li,Shaoke Zhou,Yong Xia,Lei Mou
Rapid, accurate, and cost-effective strategies for the multiplexed and quantitative detection of multiple infectious disease biomarkers are still lack in hospital. Here, we introduce a multiplexed screen-printed electrode for the simultaneous detection of three infectious disease biomarkers: HBsAg, anti-HIV, and anti-TP. The electrode modified with in-situ deposition of gold nanoparticles (AuNPs) and MXene multilayered nanosheets. Comprehensive characterization confirms the morphology and electrochemical response of the AuNPs&MXene modified screen-printed electrode (AuNPs&MXene-SPE). AuNPs&MXene composite contributes to enhance sensitivity by maximizing surface area and improving electron transfer efficiency. The AuNPs&MXene-SPE exhibits outstanding detection performance for HBsAg, anti-HIV, and anti-TP. The AuNPs&MXene-SPE sensors achieve broad detection ranges (0.05–1000 ng/mL for HBsAg, 0.35–140 ng/mL for anti-HIV, and 0.25–100 ng/mL for anti-TP) accompanied by low limits of detection (0.01, 0.11, and 0.10 ng/mL, respectively). Applied to standard serum samples, the sensors showcase precision with relative standard deviations consistently below 5.0 %, affirming their suitability for complex solution environments. These sensors offer notable advantages, including cost-effectiveness, rapid response, high sensitivity, and specificity, eliminating the necessity for expensive equipment, which open avenues for the realization of point-of-care testing (POCT).
研究领域:
电化学
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生物传感器
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纳米材料
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传染病
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即时检测
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产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 16
·3652-3661
·
IF 5.227
Qinghao Cao,Chao Chen,Jun Huang,Dantong Shen,Haolin Chen,Huiqing Zhong,Zhiming Liu,Zhouyi Guo
The flexible surface-enhanced Raman scattering (SERS) platform has ceaselessly propelled the development of point-of-care testing (POCT) in diverse fields. Herein, we report a facile strategy for the SERS-chemometric analysis of four β-blockers (bisoprolol, metoprolol, acebutolol and esmolol) based on a super-sticky mussel-inspired hydrogel SERS tape. The surface morphology and mechanical properties of the hydrogel tape can be easily controlled by adjusting the compositional ratio. The optimized tape with excellent toughness and adhesiveness allows efficient collection of analytes through a simple “paste and peel off” approach, further by spraying with silver nanoparticles using a household sprayer to instantly assemble a flexible SERS substrate, the analytes can then detected by a portable Raman spectrometer. This POCT strategy enables the identification and discrimination of four similar β-blockers with high sensitivity and accuracy in combination with the statistical algorithms. The developed SERS tape is finally utilized for the recognition of β-blockers in simulated urine solution, which realizes a limit of detection of 1.0 ng mL−1, revealing a promising potential of this SERS-based POCT for the clinical detection of doping abuse.
研究领域:
材料科学
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分析化学
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生物医学工程
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药物分析
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DOI
PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 47
·18711-18722
·
IF 7.367
Bo Zhang,Wenjuan Ma,Fuxin Li,Weichen Gao,Qian Zhao,Weipan Peng,Jiafang Piao,Xiaoli Wu,Hanjie Wang,Xiaoqun Gong,Jin Chang
Recently, immunochromatography test strips (ICTS) have been fully developed for point-of-care testing (POCT). However, the intrinsic limitations including non-quantitative detection of colloidal gold ICTS and low sensitivity of fluorescence ICTS (FICTS) significantly restrict their further application in clinical diagnosis. Taking advantages of rapid colorimetric qualitative detection and fluorescence quantitation, we designed a kind of sensitive and dual-mode magnetic FICTS (mFICTS) based on PLGA@Fe3O4 super-paramagnetic nanosphere (SPMN) probes quenching multiplex fluorescer on the test line through sandwich immunoreactions. Owing to the large number of Fe3O4 nanoparticles (about 47) encapsulated in one SPMN, about 2680 Cy5 molecules were quenched by one SPMN on the test line such that to significantly improve the analytical sensitivity as well as the detection of whole blood samples via magnetic separation. Moreover, the aggregation of black SPMN on the test line enabled a quick naked-eye screening in 3 min. For high accuracy breast cancer diagnosis, combined determination of carcinoembryonic antigen (CEA) and carbohydrate antigen (CA153) was performed on one mFICTS with the limits of detection of about 0.06 ng mL−1 and 0.09 U mL−1, respectively. Then, more than 50 clinical serum samples were investigated for high-resolution screening by mFICTS, and the results were coincident with those obtained by electrochemiluminescence immunoassay (ECLIA). Thus, the designed mFICTS is suitable for point-of-care diagnostics.
研究领域:
生物医学工程
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分析化学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyi Zhou,Siyu Fang,Luoyan Lin,Hao Feng,Tianyu Li,Lu Fang
Heparin is vital in treating thrombotic disorders; it works well as an anticoagulant coating on biomedical devices. Due to its pharmacological nature, excessive heparin can lead to thrombocytopenia, hemorrhage, and hyperkalemia, while too little may cause thrombi. Precise measurement of heparin concentration is essential for clinical practice; therefore, this study developed a heparin sensor utilizing Poly(3,4-ethylenedioxythiophene): Poly(styrenesulfonate) PEDOT:PSS/gold nanoparticle (AuNPs) nanocomposites. Heparin quantification is achieved via the specific protamine-heparin interaction, with protamine immobilized on the electrode surface. We systematically optimized the deposition conditions of PEDOT:PSS/AuNPs and the concentration of protamine and its incubation time. Following this optimization, the sensor's performance was evaluated using cyclic voltammetry (CV) in a 0.5 mM [Fe(CN)₆] 3−/4- solution over a voltage range spanning from −0.3 V to 0.7 V. The GCE/PEDOT:PSS/AuNPs/PRTM sensor achieved a heparin sensitivity of 8.113 μA·mM −1 ·cm −2 , a low detection limit of 0.05 μM, and a wide linear detection range of 0-35 μM. Under simulated physiological conditions (2.5 % human serum - containing buffer), the system exhibited a linear response to heparin (0–35 μM) and achieved recovery rates of 95.2 %–101.5 %, confirming its anti-interference capability in biological environments. The sensor demonstrated rapid response, high selectivity, wide linear range, and robust reliability, thus enabling its potential as a POCT device for real-time heparin monitoring in blood.
研究领域:
分析化学
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纳米技术
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生物医学工程
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电化学
·
临床诊断
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Yi Liu,Huanjiao Weng,Zhiwei Chen,Ming Zong,Shubin Fang,Zili Wang,Shaohua He,Yangming Wu,Jizhen Lin,Shangyuan Feng,Duo Lin
The Omicron variants of SARS-CoV-2 have been spreading globally and has never disappeared from our sight, indicating that its coexistence with humans has become a fact, and monitoring its evolution and spread remains a current task. Although polymerase chain reaction (PCR) is the most commonly used virus detection method, it requires labor-intensive and time-consuming procedures in a laboratory setting. Herein, a multichannel nanoplasmonic sensing chip based on surface enhanced Raman spectroscopy (SERS) was developed for detecting N and S proteins, as well as IgG and IgM, related to SARS-CoV-2 Omicron variants. Through a self-screening process, specific antibodies for on-site and rapid identification of important variants of concerns (VoCs) were obtained, and their binding was confirmed by protein structure analysis. The use of these S protein specific antibodies can accurately identify Omicron VoCs (BA. 5, BF.7，XBB.1.5) with the detection limit (LoD) at 0.16 pg/mL. Then, the proposed SERS array chip was integrated with a hand-held Raman spectrometer to successfully detect the Omicron subvariants in real saliva samples within only 20 minutes, greatly reducing the detection time of PCR. This sensing technology will provide a powerful and rapid point-of-care testing (POCT) method for virus diagnosis, subtype identification, and post infection antibody level monitoring.
研究领域:
生物医学工程
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分析化学
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病毒学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yu Cai,Ji-Cheng Niu,Yu-Qi Liu,Xin-Li Du,Zhi-Yong Wu
Paper-based analytical device (PAD) has received more and more attention in the field of point-of-care test (POCT) due to its low cost, portability and simple operation. Sensitivity and selectivity are two important aspects in clinical diagnostic analysis. However, low sensitivity of a PAD limits its wider application for POCT. Here we introduced a PAD that can clean and enrich the protein content from salty media with both electric field (E) and pH gradient (double E/pH gradients), with which 100-fold enrichment effect could be achieved within 70 s as demonstrated by fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) from artificial urine media. With post staining of the protein stacking band with bromophenol blue (BPB), selective colorimetric detection of human serum albumin (HSA) was achieved simply with smartphone camera in the clinically significant range of 10–300 mg‧L −1 (R 2 = 0.99) with a limit of detection (LOD) of 4.9 mg‧L −1 . Detection of microalbuminuria (MAU) of diabetic patients was demonstrated with this method without difference (ɑ = 0.01) to that by the clinical method (immunoturbidimetry). This work demonstrated the potential of this PAD method in online sample pretreatment and detection of target component from complex physiological samples.
研究领域:
分析化学
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生物医学工程
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xiaojuan Yi,Yonghua Yuan,Min Qing,Lulu Wang,Huizhen Li,Lijuan Bai
Glucose management is an important part of disease control for diabetes patients, thus the development of a rapid and real-time point of care testing (POCT) device for monitoring blood glucose is of great significance. In this work, a paper-based analytical device (PAD) is constructed by combining acetylene black (AB)-hemin complex modified filter paper as sensing platform with a smartphone as signal detector. Large specific surface area of AB decreases the self-associate and aggregate of hemin in aqueous solution, resulting in improved peroxidase-like activity of hemin. Compared with graphene oxide supported hemin, AB-hemin exhibits superior signal response on paper. Glucose oxidase (GOx) catalyzes the conversion of blood glucose to hydrogen peroxide, and then AB-hemin complex catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMB oxidized products (TMB + ) in the presence of hydrogen peroxide, thus achieving the visual detection of blood glucose. In optimal conditions, PAD provides an applicable linear range from 0.2 mM to 30 mM and a low limit of detection (LOD) (0.06 mM). Notably, the detection accuracy of the developed paper-based sensor is in good agreement with that of the commercially available blood glucose meter (p > 0.05). Moreover, the proposed PAD presents high recoveries from 95.4% to 112% (RSD ≤ 3.2%), and therefore holds great potential for glucose monitoring and diabetes diagnosis.
研究领域:
生物医学工程
·
分析化学
·
即时检测
·
糖尿病管理
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Tao Hu,Zeyang Bai,Di Wang,Ying Bai,Xiao Li,Zhonghua Ni
Prostatic cancer threatens the lives and health of numerous male patients all over the world. As a single-chain glycoprotein, prostate specific antigen (PSA) can be used to perform prostate cancer screening. At present, there are still problems such as time-consuming and low sensitivity in hospital detection. At the same time, electrochemical immunosensor technology has received a lot of attention due to its benefits, including high sensitivity, good selectivity, low usage and cost, and so on. Electrocatalysts are typically used to build electrochemical immunosensors for signal amplification in the recent years to realize sensitive detection of cancer biomarkers such as PSA. In recent years, three-dimensional nanomaterials have been widely used in the study of electrocatalysts, but the study of the sensitivity for PSA detection is still very scarce. The present work reported the application of a 3D graphene aerogel(GA)/AuNPs/Nafion electrochemical platform for highly sensitive PSA quantification. The electrochemical immunosensor has been designed by immobilizing aptamers on the screen-printed electrode (SPE) surface modified by GA/AuNPs/Nafion. Scanning electron microscopy (SEM), Raman spectra, and electrochemical characterization demonstrated the successful combination of GA/AuNPs/Nafion/SPE. Based on electrochemical impedance spectroscopy (EIS), the proposed immunosensor has demonstrated excellent electrochemical performance, exhibiting sensitive and stable responses for electrochemical detection of PSA ranging from 0.05 to 50 ng∙mL −1 with a limit of detection (LOD) of 0.0306 ng∙mL −1 . This study has great potential application in PSA detection and provides a valuable reference for point-of-care testing (POCT) of prostate cancer.
研究领域:
生物化学
·
分析化学
·
生物传感器
·
癌症研究
·
材料科学
·
电化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Yong Zhang,Zhihao Yang,Cailin Qiao,Yiyi Liu,Cuncun Wang,Xin Zeng,Jingzhou Hou,Danqun Huo,Changjun Hou
Real-time monitoring of biological markers in sweat is a valuable tool for health assessment. In this study, we have developed an innovative wearable biosensor for precise analysis of glucose in sweat during physical activities. The sensor is based on a single-atom catalyst of platinum (Pt) uniformly dispersed on tricobalt tetroxide (Co 3 O 4 ) nanorods and reduced graphene oxide (rGO), featuring a unique three-dimensional nanostructure and excellent glucose electrocatalytic performance with a wide detection range of 1-800 μM. Additionally, density functional theory calculations have revealed the synergetic role of Pt active sites in the Pt single-atom catalyst (Co 3 O 4 /rGO/Pt) in glucose adsorption and electron transfer, thereby enhancing sensor performance. To enable application in wearable devices, we designed an S-shaped microfluidic chip and a point-of-care testing (POCT) device, both of which were validated for effectiveness through actual use by volunteers. This research provides valuable insights and innovative approaches for analyzing sweat glucose using wearable devices, contributing to the advancement of personalized healthcare.
研究领域:
生物传感器
·
催化
·
材料科学
·
纳米技术
·
健康监测
·
可穿戴技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）