SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Xun Wang,Zewei Liu,Jian Yan,Daofei Lv,Xin Chen,Feng Xu,Xin Zhou,Zhong Li
Developing adsorbents with exceptional CH 4 /N 2 selectivity under high pressure is a crucial commercial requirement and a significant technical challenge for the industrial high-pressure swing adsorption (HPSA) in CH 4 /N 2 separation. Only a few adsorbents were reported to address this issue. In this work, we aimed to explore two microporous MOFs with low-polarity channels for high-pressure CH 4 /N 2 separation. The Ni(BDC)(DABCO) 0.5 and its isostructural analog Ni(TMBDC)(DABCO) 0.5 were synthesized, characterized, and examined for high-pressure CH 4 /N 2 adsorption property. Due to the lower polarity channel originating from tetramethyl groups, Ni(TMBDC)(DABCO) 0.5 exhibited an exceptional methane capacity of 4.23 mmol/g and an IAST selectivity of 5.1 for the CH 4 /N 2 binary mixture at a pressure of 10 bar, surpassing the majority of previously reported materials. The GCMC simulation revealed that the low-polarity pore surfaces in both materials exhibited a higher affinity for CH 4 molecules compared to N 2 molecules, under both ambient and high pressures. The breakthrough experiments showed that Ni(TMBDC)(DABCO) 0.5 exhibited excellent dynamic separation towards CH 4 /N 2 under both 1 bar and 5 bar, indicating great potential as an emerging candidate for the recovery of CH 4 from coal-mine methane in industrial PSA processes.
研究领域:
材料科学
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化学工程
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吸附技术
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Kang Yang,Yongjun Hu,Ning Dong,Guichi Zhu,Tingfeng Zhu,Ningjing Jiang
The accurate and highly sensitive detection of prostate specific antigen (PSA) is particularly important, especially for obese men and patients. In this report, we present a novel aptamer-based surface-enhanced Raman scattering (SERS) sensor that employs magnetic nanoparticles (MNPs) core-Au nanoparticles (AuNPs) satellite assemblies to detect PSA. The high specific biorecognition between aptamer and PSA caused the dissolution of the core-satellite assemblies, thus the concentration of functionalized AuNPs (signal probes) existing in the supernatant was on the rise with the continual addition of PSA. The aptamer-modified MNPs were used as supporting materials and separation tools in the present sensor. With the assistance of magnet, the mixture was removed from the supernatant for the concentration effects. It was found that the corresponding SERS signals from the supernatant were in direct correlation to PSA concentrations over a wide range and the limit of detection (LOD) was as low as 5.0 pg/mL. Excellent recovery was also obtained to assess the feasibility of this method for human serum samples detection. All of these results show a promising application of this method. And this novel sensor can be used for the accurate and highly sensitive detection of PSA in clinic samples in the future.
研究领域:
生物传感器
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纳米技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yuan Zhao,Han Liu,Lixia Shi,Wangwang Zheng,Xiaohui Jing
Early detection of prostate specific antigen (PSA) could reduce the mortality from prostate cancer. Electroactive nanostructures have attracted much attention due to their excellent electronic conductivity and favorable biological microenvironment. In particular, electroactive Cu 2 O nanoparticles (NPs) and Ag NPs exhibited unique oxidation signals at around -0.1 V and +0.2 V. The separated and non-interfere oxidation signals enabled them to be alternative electrochemical beacons for the establishment of ratiometric electrochemical sensor for PSA detection. Electroactive Cu 2 O NPs were designed to grow on the surface of reduced graphene oxide/Fe 3 O 4 nanosheets (rGO/Fe 3 O 4 NSs) and served as reference tags due to the stable differential pulse voltammetry (DPV) signals of Cu 2 O NPs, magnetic properties for the absorption on electrode and the accelerated electron transfer ability of rGO. In order to improve the sensitivity of detection, a large number of small electroactive Ag nanodots were dispersed on the surface of [email protected] (RF) NPs and served as detection tags. Electroactive [email protected] NPs were assembled with rGO/Fe 3 O 4 /Cu 2 O NSs for the fabrication of ratiometric electrochemical aptasensor. With the addition of PSA, aptamers modified [email protected] NPs disassembled from electrode surface, resulting in the decrease of DPV signals near +0.2 V and unchanged DPV signals at -0.1 V. This developed ratiometric electrochemical aptasensor achieved sensitive and accurate PSA detection. This proposed ratiometric electrochemical aptasensor has the advantages of easy preparation, simple structure and wide detection ranges.
研究领域:
生物化学
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纳米技术
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分析化学
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癌症研究
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产品关联
DOI
高影响因子
TALANTA
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2021
·
IF 6.057
Zipeng Yin,Ling Zhu,Zijian Lv,Meijin Li,Dianping Tang
Persistent luminescent nanoparticles (PLNPs) are a class of materials with excellent optical properties , which can continue to emit light for a long time after removing the excitation light source. This feature enables PLNPs to be used for development of biological detection modes without autofluorescence background. In this study, we prepared Zn 2 GeO 4 : Mn 2+ , Pr 3+ (ZGOMP) nanorods through a one-pot hydrothermal method . Using the pH-responsive luminescence behavior of ZGOMP, we developed an autofluorescence-free biosensor using ZGOMP as a probe and gluconic acid as a quencher to detect prostate-specific antigen (PSA). Hybridization chain reaction (HCR) and magnetic separation system were introduced in the design to achieve efficient signal amplification. Under the optimal conditions, the as-designed autofluorescence-free sensing platform showed high selectivity , and showed a good luminescence response to PSA within the linear range of 0.001–10 ng/mL at a detection limit of 0.64 pg/mL. The excellent analytical performance shows that the current strategy provides an effective platform for clinical sample analysis.
研究领域:
生物传感
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纳米技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
International Journal of Medical Sciences
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2017
·
IF 2.399
Lin Yu,Xiaofeng Wu,Min Chen,Huarong Huang,Yan He,Huaqian Wang,Dongli Li,Zhiyun Du,Kun Zhang,Susan Goodin,Xi Zheng
Background : Docetaxel is the first-line treatment for castration-resistant prostate cancer (CRPC). The limited survival benefit associated with the quick emergence of resistance and systemic toxicity diminishes its efficacy in high-dose monotherapy. YK-4-279 is a small molecule inhibitor of ETV1 that plays an important role in the progression of prostate cancer. The aim of this study was to evaluate the hypothesis that the combination of docetaxel and YK-4-279 will have a synergistic effect on inhibiting growth and accelerating apoptosis in human prostate cancer cells. Methods : Cell growth assessed using CCK-8 and trypan blue exclusion assays. Cell apoptosis was determined by morphological assessment in cells stained with propidium iodide. Standard scratch migration and Matrigel-coated transwell invasion assays were used to assess cell migration and invasion, respectively. Western blotting was used to investigate the levels of ETV1, AR, PSA, p-STAT3, survivin, Bcl-2, and p-Akt in prostate cancer cells. Results : The combination of low-dose docetaxel and YK-4-279 synergistically inhibited growth and induced apoptosis in human prostate cancer cells. The combination also more efficiently suppressed the migration and invasion of LNCaP and PC-3 cells. The combination of low-dose docetaxel and YK-4-279 caused a stronger decrease in the levels of ETV1, AR, PSA, p-STAT3, survivin, Bcl-2, and p-Akt in LNCaP cells and of p-Akt, Bcl-2, and p-STAT3 in PC-3 cells compared with either drug alone. Conclusions : These data suggest that the combination of docetaxel and YK-4-279 may be an effective approach for inhibiting the growth and metastasis of prostate cancer. This could permit a decrease in the docetaxel dose necessary for patients with CRPC and thereby lower its systemic toxicity.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
International Journal of Greenhouse Gas Control
·
2024
·
IF 4.6
Kang Shunji,Shen Zhi,Liu Baiqiang,Yi Qun,Ma Jun,Song Hao,Shen Xizhou
The petrochemical industry has a significant demand for high-purity hydrogen. It primarily obtained from the H 2 purification unit by using pressure swing adsorption (PSA) technology to recover H 2 from medium-temperature shifting gas (MTSG). The the coupled wet CO 2 separation-PSA process was introduced in this work, aiming to capture CO 2 from MTSG while simultaneously increasing the H 2 recovery rate. To enhance the desorption pressure of CO 2 separation unit in the coupled process to facilitate the storage or utilization of the captured CO 2 , firstly, a rigorous thermodynamic study was carried out to provide a theoretical basis for the development of an improved CO 2 solvent. Subsequently, a pilot-scale test was executed to validate the theoretical findings. Finally, the patented coupled process was implemented at an industrial scale for the first time. The results showed that the capacity of PSA unit for H 2 purification increased from 6800 to 12,000 tons per year and the H 2 recovery rate increased from 84.6 % to 93.6 %; the CO 2 desorption pressure increased from about 0.03 MPa (traditional Benfield process) to 0.15 MPa, which reduced the energy consumption of CO 2 compression, and the total average annual economic benefits increased by US $ 15.55 million.
研究领域:
化学工程
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环境科学
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能源技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Li Hou,Zhuangqiang Gao,Mingdi Xu,Xia Cao,Xiaoping Wu,Guonan Chen,Dianping Tang
A highly sensitive and selective impedimetric immunosensor with triple signal amplification was designed for ultrasensitive detection of prostate-specific antigen (PSA) by using anti-PSA antibody and DNAzyme-functionalized gold–palladium hybrid nanotags (Ab 2 –AuPd–DNA). The signal was amplified based on the Ab 2 –AuPd–DNA toward the catalytic precipitation of 4-choloro-1-naphthol (4-CN). DNAzyme (as a kind of peroxidase mimic) could catalyze the oxidation of 4-CN, whilst AuPd hybrid nanostructures could not only provide a large surface coverage for immobilization of biomolecules but also promote 4-CN oxidation to some extent. The produced insoluble benzo-4-chlorohexadienone via 4-CN was coated on the electrode surface , and hindered the electron transfer between the solution and the electrode, thereby increasing the Faradaic impedance of the base electrode . Three labeling strategies including Ab 2 –AuNP, Ab 2 –AuPd and Ab 2 –AuPd–DNA were investigated for determination of PSA, and improved analytical features were obtained with the Ab 2 –AuPd–DNA strategy. Under optimal conditions, the dynamic concentration range of the impedimetric immunosensor spanned from 1.0 pg mL −1 to 50 ng mL −1 PSA with a detection limit of 0.73 pg mL −1 . Intra- and inter-assay coefficients of variation were below 8.5% and 9.5%, respectively. Importantly, no significant differences at the 0.05 significance level were encountered in the analysis of 6 clinical serum specimens and 6 diluted standards between the impedimetric immunosensor and the commercialized electrochemiluminescent method for PSA detection.
研究领域:
生物传感器
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纳米技术
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分析化学
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癌症诊断
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Chuanxia Chen,Dan Zhao,Bo Wang,Pengjuan Ni,Yuanyuan Jiang,Chenghui Zhang,Fan Yang,Yizhong Lu,Jian Sun
Enzyme-triggered in situ chromogenic and/or fluorogenic reactions under accessible conditions are significant for developing enzyme activity and related spectroscopic assays. Here, we describe a facile one-pot synthetic strategy to prepare silicon-containing nanoparticles with yellow-green fluorescence and orange-red color by mixing N-[3-(trimethoxysilyl)propyl]ethylenediamine and p-aminophenol (AP) in aqueous solution at a mild temperature. Encouraged by the AP-regulated simple synthetic procedure and the generation of AP from alkaline phosphatase (ALP)-catalyzed hydrolysis of 4-aminophenol phosphate (APP), a fluorometric and colorimetric dual-readout ALP activity assay can be rationally envisioned and developed by employing APP as the substrate. In the wake of the good analytical performance of such ALP activity assay and its successful combination with enzyme-linked immunosorbent assay (ELISA), corresponding fluorometric and colorimetric dual-readout ALP-based ELISA has been constructed for highly sensitive and quantitative determination of human prostate-specific antigen (PSA), the key biomarker of prostate cancer in human serum. The convincing performance in evaluating the PSA level in serologic tests unambiguously reveals the great potential of our proposed fluorometric and colorimetric dual-channel immunoassay in early clinical diagnosis by monitoring disease biomarkers.
研究领域:
生物化学
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纳米技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·5038-5045
·
IF 3.864
Fang Tan,Yan Yang,Xiaoxue Xie,Linqian Wang,Keqin Deng,Xiaodong Xia,Xiumei Yang,Haowen Huang
The interaction between incident light and surface electrons in conductive nanoparticles produces localized plasmon oscillations with a resonant frequency that strongly depends on the composition, size, geometry, and dielectric environment. Hybrid heterostructure materials combining two or more materials in one structure represent a powerful way to achieve unique properties and multifunctionality compared to those of the individual nanoparticle components. Hybrid gold nanorods and gold nanoclusters (GNR/AuNCs) heterostructures prepared by intimate integration of GNRs with AuNCs exhibit both localized surface plasmon resonance (LSPR) property and peroxidase-like activity. It is found that the catalytic activity of the AuNC/GNR heterostructure could be remarkably enhanced by LSPR induced by photon–plasmon coupling in the visible to near-infrared (NIR) region. Meanwhile, the catalytic activity of enzyme-like AuNC/GNRs may be regulated by immunoreactions to realize specific recognition of a target analyte. Accordingly, a fast colorimetric assay within 5 min for the detection of prostate specific antigen (PSA) was developed based on a AuNC/GNRs heterostructure mask regulated by the target molecule under photon-plasmon coupling. The color intensity is inversely proportional to the PSA concentration, and quantitative analysis may be achieved in a range of 10 and 200 pg mL−1. This sensor was practically applied to detect PSA levels in prostate cancer serum samples and the determined values agreed well with those measured by the hospital using standard methods. This indicates that the AuNC/GNRs heterostructure-based assay has high accuracy for the analysis of practical samples. Moreover, the new method has the advantages of very fast determination and low sample volume requirements.
研究领域:
纳米技术
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生物化学
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癌症诊断
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Zheng Chang,Zhenyuan Duan,Junpeng Li,Xiangze Meng
In this study, hemin-functionalized polyethyleneimine/silica nanoparticles (hemin/PEI/SiO2 NPs) served as an electrochemical label for ultrasensitive detection of prostate-specific antigen (PSA), a clinically important prostate cancer biomarker. A poly(thionine)/carbon nanotube (PTh/CNTs) multilayer film was fabricated on a glassy carbon electrode (GCE) via integrated electropolymerization and electrostatic layer-by-layer (LbL) assembly, forming a {PTh/CNTs}2/PTh modified GCE with abundant amino groups. PSA capture antibodies (Ab1) were then covalently immobilized on the modified electrode through glutaraldehyde (GA) crosslinking, leveraging the film's surface-exposed amino groups. Furthermore, hemin/PEI/SiO2 NPs were synthesized through amine-carboxyl interactions between carboxyl groups of hemin and amine groups of PEI/SiO2 NPs. The immunosensor assembly employed a sandwich-type electrochemical platform using PSA detection antibody (Ab2)-conjugated hemin/PEI/SiO2 NPs. The specific antibody-antigen binding enables efficient electron transfer across the nanoscale spacing (several nanometers) between hemin/PEI/SiO2 NPs and the {PTh/CNTs}2/PTh multilayer film. In this architecture, CNTs serve as excellent electron mediators, significantly facilitating electron transport. Moreover, the high-density immobilization of hemin on PEI/SiO2 NPs significantly amplifies the electrochemical redox currents of the multilayer film through proximity effects. This enhanced electrical signal enables highly sensitive detection of target protein antigen–antibody binding events. Consequently, by correlating the enhanced reduction peak current of the immunosensor with PSA concentration, highly sensitive detection of PSA at various concentrations can be achieved. Under optimized conditions, it exhibited a linear response to logarithmic PSA concentrations (0.01 pg mL−1 to 100 pg mL−1, R = 0.9963), with an ultralow detection limit of 3.0 fg mL−1 (S/N = 3). Furthermore, the immunosensor demonstrated outstanding analytical performance in human serum samples (96.0–103% recovery, n = 3), confirming its high accuracy for clinical applications.
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Fan Xia,Jian Yang,Jingwen Chen,Ximeng Liu,Zhefan Ma,Jinlou Gu
Although hierarchically porous zeolitic imidazolate frameworks (HPZIFs) not only inherit the intrinsic architectural and chemical stabilities of their microporous counterparts but also afford open space for the efficient mass diffusion of the macromolecules involved, their rational design and construction are still challenging. Herein, HPZIFs with tailorable pore sizes ranging from 18 to 54 nm were successfully fabricated by using a newly developed soft-template-directed strategy. Our success rooted in the fact that the screened PS81–PVP44–PEO113 triblock copolymer could effectively coordinate with the metal precursor for the directed crystallization of ZIFs along surfactant assemblies. The advantages of continuous large pores and open structures in such HPZIFs were fully taken into account to serve as a bioreactor for the efficient immunoassay. The expanded large pores provided not only a significantly vast surface area to enhance the density of capture antibodies but also enough space for accommodating multiple conjugated biomolecules in one pore channel. In combination with a cascade enzyme cycle amplification strategy, a model biomarker of prostate-specific antigen (PSA) at the femtomolar level was checked with a limit of detection of 92 fM using the developed immunosensor. Specific screening on patients with prostate cancer or even benign prostatic hyperplasia was exemplified through accurately quantifying small changes of PSA concentration in clinical serum samples, prefiguring the great potential of the developed HPZIF-8 immunosensor platform for the early monitoring and diagnostics of diseases.
研究领域:
材料科学
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分析化学
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生物传感器
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生物医学工程
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2025
·Vol. 78
·期 9
·NULL
·
IF 0.9
Huipeng Zhao,Jingsi Yang,Heli Tian,Kunjie Li,Ruihong Zhao,Xiaoqing Liu,Pingxia Zhang,Xiaoxue Niu,Guicai Wu,Haoming Li
In this study, four DETA-SiO2 solid amine adsorbents were prepared by an impregnation method using diethylenetriamine (DETA) as the amine source and four types of silica with different pore structures as the carrier, and the effects of varying amine concentrations, temperatures and pressures on the CO2 adsorption performance were investigated. The results showed that the prepared N-MCM-48-C material reached a maximum CO2 adsorption of 417.45mgg−1 at 25°C and 0.5MPa. The effect of pressure on the adsorption performance was verified by kinetic calculations using the intraparticle diffusion model and the Thomas dynamic adsorption model. The presence of surfactant (CTAB) inside the carrier diluted the amine concentration, which improved the homogenisation of the amine on the carrier and increased the CO2 mass transfer efficiency and adsorption capacity. Multiple pressure swing adsorption (PSA) experiments have shown that N-MCM-48-C material has stable adsorption performance during the PSA process and the adsorption capacity can reach 300mgg−1.
研究领域:
环境科学
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材料化学
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化学工程
·
碳捕集与封存（CCS）
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吸附技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）