ANIMAL REPRODUCTION SCIENCE
·
2026
·
IF 2.6
Mengting Huang,Aixia Li,Rongjiang Ma,Yi Gao,Yuxuan Jia,Xiangyu Shen,Minghong Xie,Liang Yang,Kangsen Shen,Zheng Ao,Yiyu Zhang,Xiang Chen,Ting Gong
Male infertility is increasingly linked to impaired spermatogenesis, with emerging evidence implicating the umami taste receptor T1R1/T1R3 in male reproductive regulation. This study explored its role in regulating autophagy during spermatogenesis in Xiang pigs. Immunofluorescence confirmed T1R1/T1R3 co-localization in spermatogenic cells. Knockdown of TAS1R3 by RNAi triggered robust autophagy, characterized by increased autophagosomes, mitochondrial swelling, a significant accumulation of 2 C (diploid, spermatogonia) cells (increasing by ~21%), a corresponding reduction 4 C (tetraploid, spermatocytes) cells (decreasing by ~11%), a marked upregulation of Beclin 1 protein (by ~8.0-fold) and the LC3-II/LC3-I ratio (by ~2.9-fold), downregulation of mTOR , and suppression of key spermatogenic genes ( POU5F1 , SYCP3 , STRA8 ). Conversely, the receptors activation by its natural ligand L-glutamate (L-Glu) suppressed autophagy, reducing Beclin 1 levels and the LC3-II/LC3-I ratio to approximately 0.7-fold of the control. This activation improved cellular function, upregulated mTOR, increased 1 C (haploid, spermatids) cell populations, and downregulated STRA8 . Under H₂O₂-induced autophagy, L-Glu restored T1R3 expression, thereby reversing excessive autophagy and calcium overload, and rescuing the expression of autophagy-related ( BECN1 , mTOR ) and spermatogenesis-related genes ( SYCP3 , STRA8 ). In vivo experiments, dietary supplementation with 0.5% L-Glu significantly increased seminiferous tubule diameter and epithelium thickness in Xiang pig testes, upregulating T1R1 and the autophagy substrate p62/SQSTM1. In conclusion, T1R1/T1R3 regulates spermatogenesis by modulating autophagy. Activation by L-Glu mitigates autophagic damage and promotes spermatogenesis, offering a novel strategy for enhancing male fertility by targeting T1R1/T1R3.
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PubMed
高影响因子
Metabolites
·
2025
·Vol. 15
·期 8
·555
·
IF 3.7
Feifan Zhang,Jinhui Liang,Hongliang Zhang,Mengqi Duan,Dong Yang,Chamba Yangzom,Peng Shang
Background/Objectives:The aim of this study was to explore the effects of diets with different protein levels on the metabolite composition and metabolic pathways of the longest dorsal muscle of Tibetan pigs, in order to provide a metabolic basis for optimizing the nutritional regulation strategy of Tibetan pigs.Methods:A total of 32 healthy 180-day-old depopulated male Tibetan pigs were randomly divided into four groups and fed diets with protein levels of 10%, 12%, 14%, and 16%, respectively, with a feeding cycle of 8 weeks. The longest dorsal muscle samples were collected, and their metabolic profiles were systematically analyzed by LC-MS non-targeted metabolomics.Results:The TIC plots of the quality control samples were highly overlapped, indicating a stable instrumental detection process and good consistency of sample processing. Principal component analysis and orthogonal partial least squares discriminant analysis revealed significant metabolic differences between groups with different protein levels. A total of multiple differential metabolites was obtained based on VIP value andp-value screening, and Venn diagram analysis revealed a total of 11 metabolites among the three comparative groups, suggesting that they may have key roles in the protein regulation process. Volcano plots further clarified the number and trend of significantly up- and down-regulated metabolites in each group. KEGG pathway enrichment analysis showed that, with the elevation of protein level, the metabolic pathway response showed a tendency of expanding from basal energy metabolism to the complex network of amino acid synthesis, steroidogenesis, endocrine signaling, and detoxification pathways, especially in the high-protein-treated group.Conclusions:The study showed that different protein intake levels could significantly regulate the metabolites and key metabolic pathways in the longest muscle of Tibetan pigs, which provided theoretical support for the scientific formulation of a protein supply program to optimize the quality and growth performance of Tibetan pork.
研究领域:
动物营养
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代谢组学
·
畜牧科学
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生物信息学
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Mian Zu,Jinan Jiang,Hui Zhao,Shili Zhang,Yan Yan,Shiwei Qiu,Shuolong Yuan,Jiawei Han,Yue Zhang,Weiwei Guo,Shiming Yang
Irreversible hearing loss induced by aminoglycoside in human through local or systemic administration route negatively impacts quality of life. The aim of this work was to develop and validate an analytical method suitable for the detection and quantification of neomycin in cochlear perilymph of guinea pig after local application. The SupelMIP SPE column was used for the pre-treatment of matrix. Chromatographic separation was conducted by a reversed phase ODS column (100 × 2.1 mm, 3 μm) at 40 °C in gradient mode with 0.2‰ (v/v) HFBA in water and 0.2‰ (v/v) HFBA in acetonitrile as mobile phase , at a flow rate of 0.30 mL/min, with retention time of 3.50 and 3.62 min for internal standard tobramycin and analyte neomycin , respectively. The MS was performed with positive ionization mode, with data acquisition in Multi Reaction Monitor (MRM) mode. This method was proved to be specific, accurate (97.1–115% of nominal values) and precise (CV% < 15%). Calibration curves for matrix matched standard of neomycin ranged from 1.25 to 200 μg/mL, with LOD and LLOQ of 0.625 and 1.25 μg/mL in blank matrix. The matrix effect was corrected to (−0.1) - 1.33 by adding internal standard. The relative SPE recovery values were ≥98.9% in low, medium and high QC samples. Neomycin in matrix proved to be stable under room temperature - and −20 °C, or under three freeze-thawing cycles, or under processing as well. Finally, the proposed method was successfully applied to a toxicokinetics study of neomycin in perilymph after round window membrane (RWM) administration, which was in accordance with threshold shift of auditory brainstem response (ABR) test related to hearing loss.
研究领域:
药理学
·
分析化学
·
耳科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Kai Zhang,Xiaoqing Guo,Qingyu Zhao,Yunsheng Han,Tengfei Zhan,Ying Li,Chaohua Tang,Junmin Zhang
Dietary selenium deficiency is recognized as a global problem. Pork is the most widely consumed meat throughout the world and an important source of selenium for humans. In this study, a reliable approach was developed for analyzing selenium and its speciation in the muscles of pigs after different selenium treatments. The selenium source deposition efficiency was ranked as: selenomethionine > methylselenocysteine > selenite, and the muscle selenium content had a dose effect with selenomethionine supplementation. In total, four species of selenium were detected in the muscles of pigs and the distributions of these selenium species were greatly affected by the dietary selenium supplementation forms and levels. Selenomethionine (>70% of total selenium) and selenocystine (>11%) were the major selenium species, followed by methylselenocysteine and selenourea. Therefore, selenium-enriched pork produced from selenomethionine is a good source for improving human dietary selenium intake.
研究领域:
分析化学
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营养学
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动物科学
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PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yajun Wu,Hua Fan,Yuliang Feng,Jiasong Yang,Xiaobo Cen,Wensheng Li
Aim The aim of this study was to confirm the presence of the form deprivation myopia (FDM) guinea pig eye-gut axis and investigate the relationship between serum vasoactive intestinal peptide (VIP), lipopolysaccharides (LPS), specific gut microbiota and their metabolites. Method 20 specific-pathogen-free (SPF) guinea pigs were divided into the FDM and the control(Con) group. Following model induction, serum levels of VIP and LPS were quantified. A combination of 16S ribosomal ribosomal Ribonucleic Acid (rRNA) gene sequencing, non-targeted metabolomics and bioinformatics analysis were employed to identify disparities in gut microbiota and metabolites between the two groups of guinea pigs. Result Compared to the control group, FDM guinea pigs exhibited a significant trend towards myopia, along with significantly elevated concentrations of LPS and VIP ( p < 0.0001). Furthermore, Ruminococcus_albus emerged as the predominant bacterial community enriched in FDM ( p < 0.05), and demonstrated positive correlations with 10 metabolites, including l -Glutamic acid, Additionally, Ruminococcus_albus exhibited positive correlations with VIP and LPS levels ( p < 0.05). Conclusion The findings suggest that the Ruminococcus_Albus and glutamate metabolic pathways play a significant role in myopia development, leading to concurrent alterations in serum VIP and LPS levels in FDM guinea pigs. This underscores the potential of specific gut microbiota and their metabolites as pivotal biomarkers involved in the pathogenesis of myopia.
研究领域:
眼科学
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微生物学
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代谢组学
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PubMed
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2024
·Vol. 38
·期 15
·e9769
·
IF 1.8
Liwei Xu,Andong Zha,Xia Xiong,Dehui Sun
Rationale Succinic acid and lactic acid have been associated with diarrhea in weaned piglets. The level of succinic acid and lactic acid in serum, meat, and intestinal contents is important to elucidate the mechanism of diarrhea in weaned piglets. Methods A facile method was developed for the quantification of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC/MS/MS). The serum samples underwent protein precipitation with methanol. The meat and intestinal contents were freeze-dried and homogenized using a tissue grinding apparatus. Methanol–water mixture (80:20, v/v) was used for homogenizing the meat, while water was used for homogenizing the intestinal contents. An additional step of protein precipitation with acetonitrile was required for the intestinal contents. The resulting solution was diluted with water before being analyzed by UHPLC/MS/MS. Separation of succinic acid and lactic acid could be achieved within 3 min using a Kinetic XB-C 18 column. Results The coefficients of variation for peak areas of succinic acid and lactic acid were less than 5.0%. The established method demonstrated good linearity as indicated by correlation coefficients exceeding 0.996. Additionally, satisfactory recoveries ranging from 88.58% to 108.8% were obtained. The detection limits ( R S/N = 3) for succinic acid and lactic acid were determined to be 0.75 ng/mL and 0.02 μg/mL, respectively. Conclusion This method exhibited high sensitivity, simplicity in operation, and small sample weight, making it suitable for quantitative determination of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat. The method developed will provide valuable technical support in studying the metabolic mechanisms of succinic acid and lactic acid in pigs.
研究领域:
动物营养学
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兽医学
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分析化学
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PubMed
高影响因子
JOURNAL OF MEDICAL VIROLOGY
·
2019
·Vol. 91
·期 6
·941-948
·
IF 2.049
Manyuan Bai,Hu Dong,Xin Su,Ye Jin,Shiqi Sun,Yingpeng Zhang,Yunshang Yang,Huichen Guo
Foot-and-mouth disease (FMD) is an acute and febrile infectious disease, which can cause great economic losses. Virus-like particles (VLPs) as an advantageous antigen can induce significant specific immune response. To improve immunity of VLPs, especially, make it induce persistent immune response, the hollow mesoporous silica nanoparticles (HMSNs) as a potential nano-adjuvant were synthesized and loaded the FMD virus (FMDV) VLPs. They were injected into guinea pigs and the specific immune response was detected. The results confirmed that HMSNs/VLPs can induce persistent humoral immunity with high-level antibody titer for more than three months. HMSNs also improve the T-lymphocyte proliferation and IFN-γ induced by FMDV VLPs, and provides the ideal protection against FMDV challenge. These consequences indicated that HMSNs were good protein delivery vehicle and potential nano-adjuvant of vaccines. Highlights We synthesized Hollow mesoporous silica nanoparticles (HMSNs), as a delivery vehicle, are ideal candidate and have many unique structural features, including relatively larger surface areas, and controlled release capability, especially, good biocompatibility compared to golden nanoparticles as adjuvant. VLPs are composed of one or more structural proteins of virus, and without viral genetic material. As a new type of safe vaccine, FMDV VLPs can induce excellent immune response as traditional vaccines. To improve specific and persistent immune responses of FMDV VLPs, It is first time that HMSN was used as delivery of FMDV VLPs. The results confirmed that HMSNs can improve specific and persistent immune responses of FMDV VLPs, as new generation adjuvant.
研究领域:
纳米技术
·
免疫学
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病毒学
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兽医学
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PubMed
高影响因子
Reproductive Biology
·
2018
·
IF 1.446
Zhen Luo,Wenli Luo,Shaohua Li,Sen Zhao,Takami Sho,Xue Xu,Jing Zhang,Weina Xu,Jianxiong Xu
Intrauterine growth restriction (IUGR) remains a significant obstacle in pig production; however, information regarding the relationship between reactive oxygen species (ROS)-induced placental dysfunction and IUGR is still unknown. This study aimed to explore the placental redox status, mitochondrial content, cellular progression, and mitogen-activated protein kinase (MAPK) pathways in IUGR. Placental tissues were collected from normal intrauterine gestation (NIUG) and IUGR fetuses at delivery. Compared with the NIUG, placental ROS production, lipid peroxidation , and DNA damage were increased in IUGR. Placental mitochondrial DNA (mtDNA) content and mtDNA-encoded gene expression decreased in IUGR. Moreover, p21 phosphorylation increased, cyclin E expression decreased in IUGR cases, which showed senescence characteristics. Analysis of signaling pathways showed that the ERK1/2 phosphorylation increased whereas the p38 and JNK phosphorylation decreased in IUGR. In cultured porcine trophectoderm (pTr) cells, exogenous H 2 O 2 increased intracellular ROS production, decreased cell viability in a dose-dependent manner. Cell cycle distribution was found to arrest in S and G2/M phases. Our findings suggested that IUGR was associated with greater placental ROS and oxidative injury, which might be a factor that resulted in lower mitochondrial content, microvilli loss and senescence, and activation of MAPK pathways.
研究领域:
动物科学
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生殖生物学
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分子生物学
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细胞生物学
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PubMed
高影响因子
Toxins
·
2021
·Vol. 13
·期 12
·874
·
IF 4.546
Quancheng Liu,Fuchang Li,Libo Huang,Wenjie Chen,Zhongyuan Li,Chunyang Wang
Fumonisin B1(FB1) is the most common food-borne mycotoxin produced by theFusariumspecies, posing a potential threat to human and animal health. Pigs are more sensitive to FB1ingested from feed compared to other farmed livestock. Enzymatic degradation is an ideal detoxification method that has attracted much attention. This study aimed to explore the functional characteristics of the carboxylesterase FumDSB in growing pigs from the perspective of brain–gut regulation. A total of 24 growing pigs were divided into three groups. The control group was fed a basal diet, the FB1group was supplemented with FB1at 5 mg/kg feed, and the FumDSB group received added FumDSB based on the diet of the FB1group. After 35 days of animal trials, samples from the hypothalamus and jejunum were analyzed through HE staining, qRT-PCR and immunohistochemistry. The results demonstrated that the ingestion of FB1can reduce the feed intake and weight gain of growing pigs, indicating that several appetite-related brain-gut peptides (including NPY, PYY, ghrelin and obestatin, etc.) play important roles in the anorexia response induced by FB1. After adding FumDSB as detoxifying enzymes, however, the anorexia effects of FB1were alleviated, and the expression and distribution of the corresponding brain-gut peptides exhibited a certain degree of regulation. In conclusion, the addition of FumDSB can reduce the anorexia effects of FB1by regulating several brain-gut peptides in both the hypothalamus and the jejunum of growing pigs.Keywords:fumonisin B1;carboxylesterase FumDSB;hypothalamus;jejunum;brain-gut peptide;ghrelin;obestatin;growing pigsKey Contribution:In this study, the effects of carboxylesterase FumDSB on appetite-related brain-gut peptides in both the hypothalamus and the jejunum of growing pigs were evaluated; next, the mechanism and safety of FumDSB was explored from the perspective of brain–gut regulation, which provided a research basis for the application of FumDSB in actual production.
研究领域:
兽医学
·
毒理学
·
分子生物学
·
动物营养
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PubMed
高影响因子
Agriculture-Basel
·
2022
·Vol. 12
·期 7
·988
·
IF 3.408
Yaojun Liu,Wenqiang Shen,Tao Liu,Rainer Mosenthin,Yinghui Bao,Peng Chen,Wenbo Hao,Lihong Zhao,Jianyun Zhang,Cheng Ji,Qiugang Ma
(1) Background: L-carnosine (β-alanyl-L-histidine), a natural dipeptide, exists at relatively high concentrations in skeletal muscles, and has been shown to protect cells from adverse conditions due to its antioxidant, anti-aging, anti-glycation, and buffering properties. Satellite cells (SCs), residing on the myofiber surface, are crucial for muscle post-growth and regeneration. However, the effects of L-carnosine on muscle development of pigs in vivo, on proliferation and growth of SCs in vitro, and the relationship between SCs and muscle development have not yet been investigated. (2) Methods: The objective of this study was to assess the effect of dietary L-carnosine on growth performance andlongissimus dorsimuscle development of pigs in vivo, and to elaborate its molecular mechanisms in vitro using L-carnosine-treated SCs. (3) Results: It was shown that L-carnosine supplementation (0.2 and 2 mM) increased (p< 0.05) SC proliferation and cell percentage in the synthesis (S) phase and decreased cell percentage in the resting (G0)/first gap (G1)/phase. Moreover, average daily gain (ADG) of pigs fed diets containing 0.1% of L-carnosine was higher (p< 0.05) than that of pigs fed diets without L-carnosine, and thelongissimus dorsimuscle weight of pigs assigned to the L-carnosine treatments was 7.95% higher compared to control pigs. Both in thelongissimus dorsimuscle and cultured SCs of pigs, the Akt/mTOR/S6K signaling pathway was activated (p< 0.05), suggesting that L-carnosine improved muscle growth and SC proliferation of pigs. (4) Conclusions: Considering the important role of SCs in post-natal muscle growth, there is evidence that L-carnosine may improve muscle growth of pigs through promoting SC proliferation via activating the Akt/mTOR/S6K signaling pathway.Keywords:L-carnosine;satellite cells;proliferation;mTOR signaling pathway;muscle growth
研究领域:
动物科学
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分子生物学
·
营养学
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高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Xinran Yuan,Jialing Tang,Yulin Zhang,Zhengfeng Fang,Yong Yang,Guoquan Han,Teng Hui
This study investigated the effects of boiling, frying, and roasting on flavor compounds and their precursors in the longissimus dorsi muscle of “Duroc × Landrace × Yorkshire” pigs. HS-SPME-GC–MS, electronic nose analysis, and multivariate statistical methods (PCA, PLS-DA, and PLS-R) were used to characterize changes in free fatty acids (FFAs), free amino acids (FAAs), reducing sugars, and volatile compounds. Frying resulted in the highest total volatile content, whereas roasting was characterized by higher levels of several Maillard -derived compounds, including phenylacetaldehyde and pyrazines. Boiling showed relatively higher levels of oxidation-derived alcohols such as 1-octen-3-ol. Correlation analysis and PLS-R further revealed significant associations between specific precursor substances and key odor-active volatiles. These results provide deeper insight into the biochemical basis of processing-dependent flavor formation in ternary hybrid pork and may support the optimization of thermal processing for improved flavor quality.
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PubMed
高影响因子
Animal Nutrition
·
2021
·
IF 6.383
Xihong Zhou,Yonghui Liu,Lingyu Zhang,Xiangfeng Kong,Fengna Li
Serine and glycine are 2 of the first-affected nonessential amino acids in low crude protein (CP) diets for pigs. Therefore, we explored the effects of different dietary serine-to-glycine ratios on growth performance and lipid metabolism in growing-finishing pigs. A total of 160 crossbred healthy barrows, with a similar body weight of around 59.50 kg, were randomly allotted into 1 of 5 treatments (8 pens per treatment and 4 pigs per pen). The serine-to-glycine ratios of the 5 dietary treatments were as follows: diet A (NORMAL group), 1.18:1 (16% CP); diet B (LOW group), 1.2:1 (12% CP); diet C (S2G1 group), 2:1 (12% CP); diet D (S1G2 group), 1:2 (12% CP); and diet E (S1G1 group), 1:1 (12% CP).We found that the pigs fed a low CP diet (12% CP), when maintaining serine-to-glycine ratio at 1:2 and a total amount of 1.44%, had the same average daily gain as the pigs fed a normal CP diet (16% CP) ( P > 0.05), but they had increased intramuscular fat ( P < 0.05). Furthermore, they exhibited higher expression of genes involved in lipid oxidation ( P < 0.05), which was regulated by modulating methylation levels in the promoters of acyl-CoA oxidase 1 ( ACOX1 ) and acyl-CoA dehydrogenase medium chain ( ACADM ). When compared with the pigs fed a normal CP diet, these pigs had more oxidative myofibers ( P < 0.05), which were regulated by AMPK-PGC-1α and Calcineurin-MEF2/NFAT pathways in a coordinated manner. Our findings suggested that a dietary serine-to-glycine ratio of 1:2 is beneficial for improving meat quality in pigs fed a low CP diet.
研究领域:
动物营养学
·
脂质代谢
·
肌肉生物学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）