Arabian Journal of Chemistry
·
2023
·
IF 6
Xuhong Cai,Junbo Liu,Dadong Liang,Shanshan Tang,Bao Xu
Combining molecular imprinting technology and quartz crystal microbalance (QCM), the diethylstilbestrol-molecularly imprinted polymers (DES-MIPs) were designed. The LC-ωPBE/6-31G( d , p ) method was chosen to predict the properties of DES-MIPs in this study. The calculated results showed that the complex formed from DES and methacrylic acid with molar ratio of 1:5 and ethylene dimethacrylate as cross-linking agent had the largest amount of hydrogen bonds, the lowest binding energy, and the optimal stability property. With the guidance of calculations, the DES-MIPs were used to prepare QCM electrode by embedding method to construct the DES-MIPs-QCM sensor. The experimental results displayed that the sensor had a high binding affinity for DES when the DES-MIPs was 15 mg and the coating volume was 10 µL. The minimum detection limit of the sensor for DES was 2.63 ng/mL in the range of 50 to 350 ng/mL. The DES-MIPs-QCM sensor exhibited high recognition capacity for DES compared to its structural analogs. The sensor had been successfully used for the determination of DES in the actual water samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
Journal of Water Reuse and Desalination
·
2018
·Vol. 9
·期 1
·18-30
·
IF 0.688
Wen Yue,Zhang Xingran,Chen Mei,Wu Zhichao,Wang Zhiwei
In this study, we systematically evaluated the antibiofouling behavior of quaternary ammonium compound (QAC) blended polyvinylidene fluoride (PVDF) membrane using quartz crystal microbalance with dissipation monitoring (QCM-D) combined with flow cytometry (FCM) and confocal laser scanning microscopy (CLSM) measurements. QCM-D tests showed that the introduction of QAC reduced bacterial attachment due to the biocidal functions of QAC. FCM indicated that cell integrity of the bacteria in the suspension flowing along QAC-modified membrane surfaces during the QCM-D test was severely affected. CLSM confirmed the significantly lower attachment of bacteria and higher dead/live cell ratio onto the surface of modified membranes after the washing step in QCM-D tests. Both FCM and CLSM results validated the antibacterial behavior of QAC-modified membranes by a contact-killing mechanism, which is in agreement with that of QCM-D tests. In addition, the bacterial cells accumulated on modified membrane surface exhibited higher reversibility compared to the control membrane, indicating ease of membrane cleaning. The results highlight that the combined use of QCM-D, FCM, and CLSM can comprehensively characterize the antibiofouling behavior of membranes.
研究领域:
材料科学
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环境工程
·
膜技术
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微生物学
·
表面化学
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DOI
高影响因子
HOLZFORSCHUNG
·
2023
·Vol. 77
·期 6
·437-452
·
IF 2.4
Haonan Zhang,Qi Liu,Yanchen Zhu,Zhang Feng,Hao Ren,Huamin Zhai
In this study, representative lignin samples differing in hydroxyl content, molecular weight, linkage composition and lignin units ratio were prepared from eucalyptus wood by different isolation means and modification means: milled wood lignin (MWL), kraft lignin classified at different pH (KL), lignocresol (LC), lignoresorcinol (LR), lignopyrogallol (LP) as well as lignocresol secondary functional switched samples (LC 2 nd deriv). The structure of various lignins was characterized in detail by Fourier transform infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In order to eliminate the interference of other factors, bovine serum albumin (BSA) with relatively simple protein structure and different kinds of lignin and their derivatives were selected as the research objects. The adsorption/desorption behavior and mechanism between lignin samples and BSA were studied by quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the content of phenolic hydroxyl was the main factor affecting the interaction of lignin and BSA, but in addition, the molecular size, linkages composition and the types of lignin units also had important effects on its adsorption behavior. The research will provide theoretical guidance and all-round reliable basic data for the interaction of lignin on lignocellulose hydrolysis enzymes in industrial application.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Shuping Liu,Qingqing Zhang,Xiaohua Zhang,Cuicui Du,Jinhua Chen,Shihui Si
The misregulation of protein phosphatases is a key factor in the development of many human diseases, notably cancers. Here, based on a 100 MHz quartz crystal microbalance (QCM) biosensing platform, the dephosphorylation process of phosphopeptide (P-peptide) caused by protein tyrosine phosphatase 1B (PTP1B) was monitored in real time for the first time and PTP1B activity was assayed rapidly and sensitively. The QCM chip, coated with a gold (Au) film, was used to immobilized thiol-labeled single-stranded 5′-phosphate-DNAs (P-DNA) through Au–S bond. The P-peptide, specific to PTP1B, was then connected to the P-DNA via chelation between Zr 4+ and phosphate groups . When PTP1B was injected into the QCM flow cell where the P-peptide/Zr 4+ /MCH/P-DNA/Au chip was placed, the P-peptide was dephosphorylated and released from the Au chip surface, resulting in an increase in the frequency of the QCM Au chip. This allowed the real-time monitoring of the P-peptide dephosphorylation process and sensitive detection of PTP1B activity within 6 min with a linear detection range of 0.01–100 pM and a detection limit of 0.008 pM. In addition, the maximum inhibitory ratios of inhibitors were evaluated using this proposed 100 MHz QCM biosensor. The developed 100 MHz QCM biosensing platform shows immense potential for early diagnosis of diseases related to protein phosphatases and the development of drugs targeting protein phosphatases.
研究领域:
生物化学
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医学诊断
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药物开发
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 23
·4151
·
IF 5.1
Ziliang Fan,Miaomiao Li,Xingyu Wang,Haixia Zhou,Faraz Ahmed,Yongheng Zhu
Listeria monocytogenes(LM) poses a serious threat to food safety and public health. Current detection methods suffer from drawbacks such as expensive equipment, complex procedures, and time-consuming processes, highlighting the urgent need for a simple, rapid, accurate, and cost-effective detection approach. The bacterial metabolite 3-hydroxy-2-butanone (3H2B), due to its high abundance, can serve as a reliable biomarker for detection. Herein, ordered mesoporous silica nanoparticles (MSNs) were synthesized via a one-pot method and subsequently functionalized with APTES. The NH2-MSNs-2 exhibits extremely high sensitivity (768 Hz@50 ppm) and selectivity towards 3H2B due to its high specific surface area, abundant mesoporous structure, and weak chemical adsorption between amino groups and the 3H2B. The quartz crystal microbalance (QCM) sensor developed based on this material demonstrated outstanding performance in testing the contamination levels ofLMin food. This study provides a solid foundation for further exploring the fundamental mechanisms of QCM sensors in the real-time, non-invasive detection ofLM, while also demonstrating significant application potential in the field of food safety assurance.
研究领域:
食品安全
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生物传感器
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分析化学
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微生物检测
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Xuliang Lin,Xueqing Qiu,Duming Zhu,Zihao Li,Ningxin Zhan,Jieyi Zheng,Hongming Lou,Mingsong Zhou,Dongjie Yang
Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL–PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL–PEG, glucose yield of corn stover increased. EHL–PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8–5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL–PEG, while that with PEG4600 was 54.2%. Effect of EHL–PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL–PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL–PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.
研究领域:
生物质转化
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酶工程
·
高分子化学
·
表面科学
·
可再生能源
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DOI
高影响因子
Food Quality and Safety
·
2025
·
IF 4.4
Lu Xinghua,Liu Suqi,Zhang Lu,Wang Chenglong,Wang Zhenhe,Pang Linjiang,Lu Guoquan,Cheng Jiyu,Chen Wei,Guan Yuge
Citronellol is a kind of terpene produced by plants in response to external stress, thus can be used as a gas biomarker to detect black spot Ceratocystis fimbriata infection in sweetpotato. However, many contemporary analytical methods, exemplified by gas chromatography-mass spectrometry (GC-MS), are technically demanding, time-consuming, and require complex sample preparation procedures. In this study, a quartz crystal microbalance (QCM)-based gas sensor fabricated via a surface molecular imprinting technique was modified with a Co/Zn-ZIF@MIP composite, in which cobalt-zinc bimetallic ZIF (Co/Zn-ZIF) served as the support material. A linear relationship was observed between the frequency shift and citronellol concentrations ranging from 0.88 to 22 mg/L, with a sensitivity of –6.08 Hz/(mg·L) and a limit of detection (LOD) of 1.35 mg/L. This result indicated that this sensor has excellent selectivity for citronellol and demonstrates high repeatability, as evidenced by R² value of 0.97. In evaluations with real samples, the sensor reliably identified citronellol among the complex volatile organic compounds (VOCs) emitted from black spot-infected sweetpotato, indicating a high level of selectivity. Our research achieved the rapid characterization of sweetpotato black spot disease within 4 min and provided new insights into the development of QCM-based gas sensors for the rapid assessment of agricultural product quality and safety.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yansi Cao,Yu Fan,Zhiheng Ma,Zhixuan Cheng,Qun Xiang,Zhiming Duan,Jiaqiang Xu
There is an urgent need for effective and portable gas sensors toward organophosphorus (OP) vapors in the areas of anti-terrorism and pesticides residue monitoring. Herein, we report a series of urea-functionalized SBA-15 (UXs/SBA-15: U1/SBA-15, U2/SBA-15 and U3/SBA-15) prepared by post-grafting methods and their applications in trace-level dimethyl methylphosphonate (DMMP, lab simulate of toxic OP agents) sensing by means of quartz crystal microbalance (QCM) transducers. U3/SBA-15 based QCM sensors exhibited excellent responses and remarkable selectivity toward DMMP vapors. A frequency shift (Δ F ) of −50 Hz was achieved when the DMMP vapors reached 30 ppb. And the limit of detection (LOD) of QCM sensor modified with U3/SBA-15 was found at 12.25 ppb. Moreover, the simulated adsorption enthalpies (Δ H ) of UXs and DMMP molecules were calculated with Gaussian 09 to investigate the sensing mechanism. The results indicated the existence of chemisorption between UXs and DMMP molecules caused by hydrogen-bonding interactions, among which a minimum Δ H (−71.96 kJ mol −1 ) between U3 and DMMP molecule was found, resulting a maximum response to DMMP compared with U1-DMMP (−52.53 kJ mol −1 ) and U2-DMMP (−57.01 kJ mol −1 ). Therefore, the well-defined UXs/SBA-15 lead to a great potential on the detection of OP vapors.
研究领域:
材料科学
·
化学传感
·
分析化学
·
环境监测
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Ye Zhu,Zhixuan Cheng,Qun Xiang,Xikun Chen,Jiaqiang Xu
Mesoporous TiO 2 -SiO 2 functionalized with p-hexa-fluoroisopropanol aniline (HFIP) was designed and synthesized to achieve highly sensitive and selective sensing toward nerve agent simulant dimethyl methylphosphonate (DMMP) due to a hydrogen bonding between target DMMP and TiO 2 -SiO 2 -HFIP. The sensing experiments were conducted by coating compounds onto quartz crystal microbalance (QCM) transducers. It was found that the observed frequency shifts gradually and turned saturated in the range from 10 to 60 ppm. Gas sensing test results revealed that sensors had enhanced sensitivity due to the decoration of HFIP on TiO 2 -SiO 2 in a ppb level. Furthermore, when the doping of HFIP reached 20 wt%, the sensors achieved the best sensing performance. In conclusion, the QCM sensor coated with mesoporous TiO 2 -SiO 2 -HFIP exhibited fast response, excellent reversibility and high selectivity to nerve agent stimulant DMMP vapor, implying that mesoporous TiO 2 -SiO 2 -HFIP may be a good candidate for sensing DMMP.
研究领域:
材料科学
·
化学传感
·
纳米技术
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产品关联
DOI
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Yong Wang,Chunxiao Yan,Weiye Liang,Qiang Zhang,Gang Cheng,Jicheng Dong,Lin Jin,Huan Tang,Fang Cheng,Lijun Zhang
Orthokeratology (OK) lenses for myopia correction are susceptible to biofouling by tear-derived biomolecules, escalating the risks of ocular infection and inflammation. Prevailing studies that rely on end point protein quantification fail to capture the real-time kinetics of fouling formation. Here, we fabricated a UV pressure-assisted polymer-grafted quartz crystal microbalance with dissipation monitoring (QCM-D) sensor exhibiting exceptional stability, nanoscale smoothness (RMS roughness ≈2 nm), and interfacial peel resistance. This platform enables in situ tracking of adsorption/desorption kinetics for four critical tear components: native/denatured lysozyme and oxidized/native lecithin. Key findings reveal a flow-dependent fouling behavior, wherein low flow rates increase biomolecule adsorption by 37–80% compared with higher flows. We further identify denatured lysozyme and native lecithin as resilient contaminants characterized by a stronger deposition affinity and pronounced resistance to elution. Quantitative screening of multipurpose solutions (MPSs) demonstrates that MPS #2 achieves 20–100% elution rate across biomolecules, outperforming commercial benchmarks. By leveraging dissipation–frequency (D–F) analysis, we clarify the fundamental mechanisms of biofouling formation at the molecular level. Collectively, this work establishes three critical advances: (1) a real-time biofouling diagnostic platform for OK lens interfaces, (2) molecular design principles for antifouling materials based on adhesion remodeling theory, and (3) an accelerated MPS formulation screening paradigm for ocular device safety.
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Tao Wen,Weitao He,Qiyi Nie,Zhongliang Gong,Dapeng Li,Liang Wen,Hailong Peng
The characteristic substances in citrus volatile organic compounds (VOCs) are associated with the infestation of the Bactrocera dorsalis (Hendel), which provides a noninvasive evaluation method to discriminate infested citrus. This paper developed an olfactory detection system based on a quartz crystal microbalance (QCM) sensor array and classification algorithms to identify the citrus infested with B. dorsalis . Six characteristic substances, including d -limonene, myrcene, α-pinene, decanal , linalool , and β-ocimene, which vary significantly after B. dorsalis infestation were selected as template molecules to prepare molecularly imprinted polymers (MIPs) and modify QCM respectively. The experimental results show that the prepared MIPs-QCM sensors had sensitivity in the range of 0.043–0.070 Hz/(mg/m 3 ), and their stability and reproducibility were above 93.1%. Four sensors that contributed to the classification were screened by a stepwise discriminant analysis. Afterward, Bayesian optimization was employed to optimize the hyperparameters. The accuracy of the optimized support vector machine (SVM) reached 94.17%. The olfactory detection system developed in this study enables the discrimination of citrus infested with B. dorsalis , which may have potential applications in the field of post-harvest treatment of citrus.
研究领域:
植物保护
·
食品科学与技术
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DOI
高影响因子
MINERALS ENGINEERING
·
2025
·
IF 5
Ting Xiao,Wenjie Zou,Congzhong Tian,Wentao Hu,Zhijun Zhang,Chengyan Xu,Fang Zhou
Green and efficient flocculants are crucial for the effective treatment and recycling of mine wastewater. Herein, the flocculation performance and mechanism of a novel amphoteric chitosan-based flocculant CMC-g-CPAM on kaolinite suspensions were investigated through settling tests, zeta potential measurements, floc size distribution, quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM) measurements. Settling tests results revealed that CMC-g-CPAM displayed exceptional flocculation performance, achieving a settling rate of 18.21 m/h for kaolinite at natural pH, higher than that of chitosan-grafted-cationic acrylamide Chi-g-CPAM (11.19 m/h). The in-situ adsorption layer of CMC-g-CPAM on silica and alumina surfaces was more rigid than that of Chi-g-CPAM. Subsequently, more kaolinite particles were flocculated by CMC-g-CPAM, and formed a denser floc layer. The adsorption amounts of CMC-g-CPAM and Chi-g-CPAM on silica surfaces were found to be greater than those observed on alumina surfaces at natural pH. This phenomenon was related to the different surface charges of silica and alumina, electrostatic interactions between the polymers and surfaces. From AFM measurements, an attraction between the CMC-g-CPAM functionalized probe and the functional substrate as they approached each other and a weak adhesion force among CMC-g-CPAM (0.31 nN over 50 nm) were observed. These indicated the presence of intermolecular interactions among CMC-g-CPAM, which contributed to the formation of compact flocs. In contrast, an obvious repulsion was detected when the Chi-g-CPAM functionalized probe approached the functional substrate, inhibited the entanglement of polymer chains. Meanwhile, the interactions between multiple molecules and the substrate displayed a large adhesion force (2.10 nN over 150 nm), attributed to the less rigid adsorption layer of Chi-g-CPAM. Our findings would provide new insights into the design of flocculants and effective wastewater treatment.
研究领域:
环境工程
·
高分子科学
·
胶体与界面科学
·
矿物加工
·
废水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）