SN Applied Sciences
·
2019
·Vol. 2
·期 1
·1-11
·
IF 0
Li Genrong,Yu Wenqin,Xiao Zhaojing,Long Mei,Tong Lanyan,Qiu Yue
A rapid, sensitive, and practical QuEChERS method coupled with gas chromatography–mass spectrometry was developed for the simultaneous determination of 16 chemical adjuvant residues in samples of fruits (apple, nectarine, grape) and vegetables (tomato, cucumber, spinach). All target compounds were separated in less than 16 min. Samples were extracted with acetonitrile, and the types of extraction salt and cleanup sorbent were optimized. The optimized method was validated by evaluating the linearity, accuracy, precision, and matrix effect. The recoveries of the 16 pesticides adjuvants ranged from 61.2 to 116.3% with relative standard deviations lower than 11.6%. Limits of detection ranged from 0.21 to 3.02 μg/kg, and limits of quantification ranged from 0.71 to 10.07 μg/kg. Finally, the developed method was successfully employed to identify and quantify pesticide adjuvant residues in the analysis of 30 real fruit and vegetable samples.
研究领域:
分析化学
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食品科学
·
环境科学
✓ 已验证
产品关联
DOI
FOOD CHEMISTRY
·
2018
·
IF 4.946
Zhoufei Luo,Jing Lu,Haipu Li,Yi Tu,Yuehao Wan,Zhaoguang Yang
A new, sensitive, and rapid method based on the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) approach and air-assisted liquid-liquid microextraction (AALLME) technology was developed for the determination of 20 endocrine-disrupting compounds (EDCs) in fish by high-performance liquid chromatography–tandem mass spectrometry. The method first integrates AALLME into QuEChERS to achieve clean-up and enrichment of the EDCs in one step. A self-made glass tube was enfolded with plasticine to withstand the high centrifugal force. The established method was developed by optimization of the parameters. High linearities (R 2 > 0.9924) and recoveries (78.2–118.6%) at three spiked levels (5, 10, and 20 ng g −1 ), and low relative standard deviation values (1.1–14.5%) and limits of detection (0.03–0.80 ng g −1 ) were obtained. The method comparison shows that the proposed method is superior as it involves less organic solvent usage, simple operation and high efficiency. This method was successfully applied to different fishes for analyzing EDCs.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 407
·期 30
·8959-8970
·
IF 3.436
Zhao Fengchun,Hu Chunyan,Wang Huimin,Zhao Longyu,Yang Zhengyou
To develop a broad-specificity immunoassay for organophosphorus pesticides (OPs), a broad-specificity monoclonal antibody (MAb) for OPs against a generic hapten, O , O -diethyl O -(3-carboxyphenyl) phosphorothioate with the carboxy group in the meta position of the benzene ring, was produced. Eight haptens were prepared and covalently attached to ovalbumin (OVA) for use as coating antigens, and the optimum coating antigen was selected. Then, a sensitive and broadly class selective competitive indirect enzyme-linked immunosorbent assay (ciELISA) based on the MAb and the optimum coating antigen (hapten H-OVA, possessing an O , O -dimethyl generic structure and linked through a linear spacer arm) was developed and optimized. The MAb developed in this study showed quite different cross-reactivity and selectivity compared to previously produced anti-OPs broad-specificity MAbs. Specifically, the MAb showed high and uniform sensitivity to seven O , O -diethyl OPs and six O , O -dimethyl OPs. With the optimum ciELISA, the IC 50 values of the 13 OPs were determined as 23.1∼151.2 ng mL −1 . The average IC 50 and coefficient of variation (CV) for the IC 50 values of the 13 OPs were 74.6 ng mL −1 and 33.9 %, respectively. For the recovery study, a QuEChERS approach based on dispersive solid-phase extraction (d-SPE) was implemented to decrease the matrix effects of vegetable and fruit samples. The recoveries of six representative OPs from the spiked samples ranged from 89.4 to 135.5 %; the CV ranged from 3.5 to 15.7 %. The ciELISA was also applied to real samples, followed by confirmation with gas chromatography–tandem mass spectrometry (GC–MS/MS) analysis. The results demonstrated that the ciELISA is suitable for monitoring OP contamination in vegetable and fruit samples.
Development of a MAb-based immunoassay for determination of organophosphorus pesticides pretreated with QuEChERS
研究领域:
分析化学
·
环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Hui Jin,Jie Hou,Yuyu Ruan,Jiang Xu,Kun Yang,Daohui Lin
Antibiotics are commonly released into paddy fields as mixtures via human activities. However, the simultaneous extraction and detection of these chemicals from multiple media are technically challenging due to their different physicochemical properties, resulting in unclear patterns of their transport in the soil-rice system. In this study, a “quick, easy, cheap, effective, rugged, and safe” (QuEChERS) method was developed for the simultaneous analysis of 4 tetracyclines (TCs) and 4 fluoroquinolones (FQs) in the soil and rice tissues from a local poultry farm, and thereby the distribution patterns of the target antibiotics in the soil-rice system and their risk levels to the soil were analyzed. After parameter optimization, the calibration range used for the target antibiotics was 0.1–50 μg/L and each calibration curve was linear with a coefficient of determination ( R 2 > 0.995); The QuEChERS method achieved satisfactory recovery rates (70.3-124.6%) along with sensitive detection limits (0.005-0.21 ng/g) for TCs and FQs in the soil, root, stem, leaf, and grain. Among the 8 antibiotics, enrofloxacin (ENX), ciprofloxacin (CIP), oxytetracycline (OTC), and doxycycline (DOX) were detected around a poultry farm. The four antibiotics in the collected paddy soils around the poultry farm ranged from 7.1 ng/g to 395.5 ng/g. Notably, ENX and DOX had higher ecological risks (risk quotient values >1) than CIP and OTC in soil. ENX, CIP, and DOX were highly enriched in rice roots with concentrations up to 471.9, 857.3, and 547.4 ng/g, respectively, which were also detected in rice aboveground tissues. The findings may provide both technical and practical guidance for the understanding of antibiotic environmental behavior and risks.
研究领域:
环境科学
·
农业化学
·
土壤学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2025
·Vol. 36
·期 1
·e70031
·
IF 3.4
Xiaotian Wang,Yeerken Aheyeli-kai,Huakun Ying,Jiangli Lin,Yulu Luo,Hongying Ma,Zhongyang Li,Zhen Su
Triphenylmethane dyes have a wide range of applications in textiles, pharmaceuticals, food, and other fields. Malachite green (MG), fuchsin acid (FA), and crystalline violet (CV) were typical representatives of triphenylmethane dyes, and they are carcinogenic and mutagenic to aquatic organisms and environment. However, they need to be separated and enriched for their accurate quantification due to their low content. Therefore, it is important to develop a rapid and accurate enrichment method to monitor these dyes in aquatic systems for human health. In this paper, a novel QuEChERS adsorbent porous ZIF67/PAA/PES composite microspheres were prepared and applied for the rapid adsorption of triphenylmethane dyes in wastewater and fish samples, which improved the adsorption capacity and the analytical sensitivity of the targets. The experimental results demonstrated that the porous ZIF-67/PAA/PES composite microspheres exhibited excellent selective adsorption capacity of MG, FA, and CV, and after six adsorption–desorption cycles, the material adsorption amount can reach more than 90% of its original adsorption amount in wastewater. In addition, the porous ZIF67/PAA/PES composite microspheres were applied as QuEChERS adsorbents for the enrichment of MG and CV dyes in fish, and the adsorption rates of the dyes were 99.11% and 99.03%, with the RSDs of 1.58% and 1.27%, respectively. The limits of detection were 0.0093 and 0.0127 mg/L for MG and CV. Through the Langmuir adsorption isotherm models to predict the MG, FA, and CV of the maximum adsorption capacity were 5417.3622, 2119.9011, and 1654.1026 mg/g, respectively. Thus, the novel porous ZIF67/PAA/PES composite microspheres are more advantageous as QuEChERS adsorbents for sample pre-treatment and have very important research significance.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Lingfei Ma,Yanfei Wang,Haipu Li,Fangyuan Peng,Bo Qiu,Zhaoguang Yang
Monitoring neonicotinoid residues in grains is of significant interest for the proper assessment of pesticide exposure to human. The quick, easy, cheap, effective, rugged, and safe extraction method combined with dispersive liquid-liquid micro-extraction (QuEChERS-DLLME) was developed for extracting, purifying, and concentrating seven common neonicotinoid pesticides from the grains (rice, millet, and maize). Water and acetonitrile were used in tandem as extractants in QuEChERS, while water, acetonitrile, and trichloromethane in DLLME acted as the external phase, dispersant, and extractant, respectively. Comprehensive consideration of the single factor test and response surface method to optimize parameters including type and volume of extractants and dispersant. The evaluation showed that the QuEChERS-DLLME method held excellent linearity ( R 2 > 0.99). The limits of quantitation ranged from 0.003 to 0.08 µg kg −1 for the seven insecticides. The recoveries were in the range of 62–118%, and good reproducibility was obtained with a relative standard deviation below 15%.
研究领域:
农业化学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Dan Fei,Mengzhi Wang,Yujie Hou,Min Xie,Yaomin Zhou,Youhua Zhao,Lei Wu,Jun Xu
The indiscriminate use of organophosphorus pesticides poses a great threat to human health because of harmful substance bioaccumulation. We developed a rapid and reliable method for the simultaneous determination of chlorpyrifos, isocarbophos, methamidophos, omethoate, and triazophos in procine hair. The targets were separated by a C18 column (2.1 × 100 mm, 1.8 µm) and analyzed by electrospray ionization using the positive mode of a triple quadrupole mass spectrometer. The analytes had linear relationships from 1.0 to 100 µg/kg. The correlation coefficients were all greater than 0.998. The limits of detection ranged from 0.4 to 0.9 µg/kg, and the limits of quantification from 1.2 to 2.0 µg/kg. The recoveries were from 76.1% to 106.2%. The intra-assay and inter-assay precision rates were from 1.6% to 4.4% and 2.3% to 6.9%, respectively. The intra-day and inter-day accuracy ranged from 2.5% to 9.3% and 3.2% to 8.9%, respectively. This method was a valid tool to monitor organophosphorus pesticides in breeding and livestock products.
研究领域:
分析化学
·
环境科学
·
兽医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2016
·
IF 2.687
Yanyan Xing,Wenting Meng,Wanyang Sun,Dongxiang Li,Zhiguo Yu,Ling Tong,Yunli Zhao
A high-throughput method for simultaneous qualitative and quantitative analysis of 21 mycotoxins in Radix Paeoniae Alba (RPA) was developed by coupling the modified QuEChERS method with ultra-high performance liquid chromatography quadrupole linear ion trap mass spectrometry (UHPLC-QqLIT-MS). The 21 mycotoxins were extracted and cleaned up using QuEChERS-based procedure, then further separated on a C18 column and detected by a hybrid triple quadrupole linear ion trap mass spectrometer equipped with electrospray ionization source in the multiple reaction monitoring-information dependent acquisition-enhanced product ion (MRM-IDA-EPI) mode. Under this technique, 13 mycotoxins were detected using acetonitrile and water containing 0.1% formic acid as the mobile phase in positive mode while the other 8 mycotoxins were detected using acetonitrile and water containing 0.1% ammonia as the mobile phase in negative mode. The calibration curves of all analytes showed good linearity ( r 2 > 0.995) within test ranges. The limits of detection and quantification ranged from 0.031 to 5.4 µg/kg and 0.20 to 22 µg/kg, respectively. Additionally, recoveries were all above 75.3% with relative standard deviations within 15%. The method proposed herein with significant advantages including simple pretreatment, rapid determination as well as high sensitivity, accuracy and throughput would be a preferred candidate for the determination and quantification of multi-class mycotoxin contaminants in real samples.
研究领域:
中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Dejin Wang,Xingqiang Wu,Jingchun Mao,Zongyi Wang,Yujie Xie,Xudong Wu,Kaixuan Tong,Hongping Man,Chunlin Fan,Hui Chen
In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges ( R 2 > 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Youzhi Su,Jianjiang Lu,Xuwei Guo,Jun Liu,Haibin Fu,Li Yu
A modified QuEChERS purification–liquid chromatography-tandem mass spectrometry method was developed to simultaneously detect six morpholine pesticide residues in plant-derived foods. The samples were extracted using acetonitrile and purified using three adsorbents, namely, graphitized carboxyl MWCNTs , C 18 , and SAX. The limit of quantitation was 0.07–0.75 µg/kg, and the average recoveries ranged from 71.3 % to 113.8 %. The precision ranged from 1.2 % to 13.8 % at four spiked concentrations of 0.5, 1, 5, and 10 µg/kg. The method was applied to analyze 540 samples obtained from 17 types of plant-derived foods collected in Yining, China. Dimethomorph and flumorph were detected in nine types of matrices, and the residue levels did not exceed the maximum residue limits (MRLs) set by the national standard GB 2763–2021. Furthermore, we assessed the risk of acute and chronic exposure to the tested pesticides. The pesticide residue levels were acceptable in all the samples.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Suo Decheng,Fan Xia,Xiao Zhiming,Liyang,Wang Peilong
A simple and accurate method of ultra high performance liquid chromatography (UHPLC) coupled with quadrupole-high field Orbitrap high-resolution mass spectrometry (QE HF HRMS) has been developed for analyzing 8 trace-level (µg/L) carbapenems in milk. Mass spectrometry conditions, chromatographic conditions, extraction solvent and QuEChERS procedures were optimized for determination of 8 carbapenems in milk. Samples were extracted and purified by modified QuEChERS procedure. Good separation for 8 carbapenems s was achieved with a PFP column at 8 min. Method validation results showed the linear ranges are 1–100 µg/L to 10–1,000 µg/L, the correlation coefficients are more than 0.995, and the recoveries of spiked samples are 79.3%–104% with a relative standard deviation less than 15%. This method successfully applied to monitor residue of carbapenems in real milk, four kinds of carbapenems have been detected in 8 sample of 79 collected milks with concentration ranged between 15 µg/L∼3,325 µg/L.
研究领域:
分析化学
·
食品安全
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoli Guo,Tingze Ren,Jianchun Ji,Yan Yang,Xin Di
In this work, a combination of QuEChERS and dispersive micro-solid phase extraction (D-μ-SPE) based on dissolvable layered double hydroxide (LDH) has been established for preconcentration and enrichment of sulfonylurea herbicides (SUHs) in wolfberry samples. The QuEChERS was used for extraction and purification of SUHs, followed by D-μ-SPE for further enrichment of targeted analytes to obtain superior extraction performance. Dissolvable LDH nanosheets were used as absorbents, thus eliminating the elution step needed in traditional D-μ-SPE. The main influence experimental variables including pH of sample solution, amount of LDH, vortex time and volume of acidic solution were optimized in detail. Under the optimized conditions, the proposed method shows high precision (RSDs < 12 .7%), low limits of detection (0.01–0.5 ng/g) and limits of quantitation (0.1–2.0 ng/g), acceptable recovery (80.1%–97.1%), thus making it a good alternative analytical strategy for the determination of SUHs residues at the low nanogram per gram range with desirable sensitivity.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）