FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Han Qiu,Angxin Song,You Luo
Rosa roxburghii Tratt fruits are rich in polysaccharides. Two novel polysaccharides, RRTP-1 and RRTP-2, were purified and characterized as pectin using HPLC, FTIR, GC-MS, and NMR analysis. RRTP-1 was primarily composed of homogalacturonan (HG), featuring a linear main chain with various branching structures including galactose, glucose, arabinose, and mannose residues. RRTP-2 was also a highly branched HG with significant contributions from galactose, rhamnose and arabinose. Compared to RRTP-2 (262.35 kDa, DE 22.03 %), RRTP-1 exhibited a lower molecular weight (179.05 kDa) and a higher degree of esterification (DE, 41.10 %). Both of them were efficiently utilized by gut microbes to produce butyric acid and lower pH. Their structural differences led to distinct gut microbiota composition. RRTP-1 selectively enriched Phocaeicola , Faecalibacterium , and Bifidobacterium , whereas RRTP-2 selectively enriched Limosilactobacillus , Lachnospira , and Coprococcus . Despite structure differences in RRTP-1 and RRTP-2, the keystone microbes and enzymes involved in their degradation exhibited similarities. Bacteroides and Megamonas emerged as dominant contributors. Glycoside hydrolases and carbohydrate esterases were identified as the primary enzymes facilitating their breakdown. These findings suggest that the fine structure of pectin exerts a selective effect on fermenting consortia. Furthermore, both RRTP-1 and RRTP-2 show promise as effective prebiotics.
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Food Quality and Safety
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2026
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IF 4.9
Lu Yanling,Bi Huimin,Feng Yiting,Yang Jiaojiao,Han Long,Guo Lei,Fan Fangyu
ObjectivesTo enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil (RSO) and β-carotene (βC), sodium alginate (SA) and chitosan (CS) were utilized for bilayer surface modification. In this study, the proliposomes containing RSO and βC are referred to as Ps, while the modified formulations are denoted as sodium alginate–chitosan-modified proliposomes (SA-CS-Ps).Materials and methodsThe physicochemical properties, structural characteristics, thermal stability, microstructure, rheological behavior, storage stability, and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps (CS-Ps).ResultsThe particle size and turbidity of Ps increased with the addition of modification layers, reaching 563.73 nm and 0.697, respectively, for SA-CS-Ps. CS and SA formed a protective bilayer through electrostatic interactions, which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress. Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76 °C, respectively, compared with that of unmodified Ps. During storage, the retention rates (RRs) of RSO and βC followed the order SA-CS-Ps>CS-Ps>Ps, whereas malondialdehyde and peroxide values exhibited the opposite trend. Elevated temperatures accelerated oxidation, thereby compromising the protective effect of the carriers. After 24 d at 4 °C, SA-CS-Ps demonstrated superior stability, with malondialdehyde of 0.016 μg/mg, peroxide value of 2.697 mmol/kg, and RRs of RSO of 97.15% and βC of 96.37%. The oxidation kinetics conformed to a first-order reaction model.ConclusionsGiven its enhanced protection against degradation and stability, SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds. This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.
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Food Chemistry-X
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2026
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IF 8.2
Mingyu Zhu,Zhiyun Peng,Guangcheng Wang
The fruit of Rosa roxburghii Tratt. (RRT) is frequently eaten in Guizhou Province, China. Thirty compounds from RRT fruit were identified by the bioaffinity-ultrafiltration method. Among them, quercetin, diosmetin, luteolin, and apigenin (IC 50 = 4.80 ± 0.12 μg/mL, 52.62 ± 0.26 μg/mL, 17.51 ± 0.27 μg/mL, 33.24 ± 0.32 μg/mL) exhibited significant α-glucosidase inhibitory activities. Based on multispectral and molecular docking analyses, these active compounds bind to the active site of α-glucosidase via at least four hydrogen bonds and van der Waals forces, thereby altering its hydrophobic environment and secondary structure. in vivo studies showed that compared to the AUC of postprandial glucose in the model (2809.33 ± 64.84 mM·min) and in the acarbose group (2525.40 ± 54. 28 mM·min), these compounds decreased postprandial blood glucose levels. Long-term administration of apigenin additionally improved lipid metabolic disorders. These results support the development of RRT fruit as a hypoglycemic functional food.
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ACS Sustainable Chemistry & Engineering
·
2023
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IF 8.4
Jian-Dong Wang,Qi Gu,Zi-Hui Cai,Guo-Sheng Liu,Quan Zhou,Li-Na Fu,Shi-Liang Yang,Su Zhang,Yujie Fu
In this study, a green CO2-responsive switchable hydrophilic solvent (SHS) was screened in the strategy of extracting and enriching active components with different polarities from a plant matrix. The mechanism of solvent switching was confirmed by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. Furthermore, the function of SHS polarity switching was proven by Kamlet–Taft parameters, and the polarity parameter π* of the solvent increased from 0.34 to 1.01 after introducing CO2. The solvent system was used to extract the active ingredients from Rosa roxburghii; a series of single factor experiments were conducted using the Box-Behnken design in order to optimize the process. In addition, the phenomenon of the SHS/water two-phase system being heated by ultrasound to produce an O/W micellar system was observed, which provided solvent solubilization. Moreover, after 5 cycles, the solvent still ensured a high extraction capacity of the target components. In addition, density functional theory (DFT) and molecular dynamics theory revealed that van der Waals forces and hydrogen bonding made the SHS more biocompatible with the target compounds and that van der Waals forces played a significant impact. In conclusion, the current process offered an ecologically acceptable, promising, and long-term alternative approach for simultaneously extracting and enriching hydrophilic and hydrophobic phytochemicals from plant materials.
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高影响因子
Frontiers in Chemistry
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2020
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IF 3.693
Zhang Qianchun,Liang Junyi,Zhao Li,Wang Yan,Zheng Yuguo,Wu Yun,Jiang Li
A novel carbon quantum dots (CQDs) were successfully synthesized by one-step hydrothermal reaction using Rosa roxburghii as a biomass-based precursor. The CQDs have an average size of 2.5 nm and a narrow size distribution. They display strong blue fluorescence with a quantum yield of 24.8% and good biocompatibility. Notably, these CQDs were capable of detecting trace o-nitrophenol in surface water and sewage with high sensitivity and specificity. The linear range is 0.08–40 μmol/L, and the limit of detection is 15.2 nmol/L. Furthermore, this CQDs was successfully applied for o-nitrophenol analysis in river water and sewage samples. Additionally, Hep3B cells, a human hepatocellular carcinoma cell line, can be easily imaged with high resolution using the as-prepared CQDs as nanoprobes. These results reveal that the as-prepared CQDs have potential applications for detecting o-nitrophenol and cell imaging.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 3
·e70105
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IF 3.8
Heting Zhou,Xinyue Zheng,Shaolin Huang,Xiaomeng Wang,Ting Zhou,Shuwen Zhang,Yihan Ling,Wenxi Wang,Xingjie Li,Shouqian Li,Yongmei Xie,Wenya Yin
Pulmonary fibrosis (PF) is a chronic and progressive lung disease, and oxidative stress plays a critical role in its pathogenesis. Rosa roxburghii Tratt, known for its anti-inflammatory and antioxidant properties, has been shown to alleviate fibrosis. This study aimed to explore whether two Rosa roxburghii fermentation broths (RRFBs) (with different proportions) could attenuate bleomycin (BLM)-induced PF in mice and to elucidate the molecular mechanisms. The results revealed that RRFBs reduced structural lung damage, collagen deposition, and lung inflammation. RRFBs also suppressed fibrotic markers (Collagen I, Vimentin, and α-SMA) while enhancing epithelial marker E-cadherin expression. Additionally, RRFBs alleviated BLM-induced oxidative stress and apoptosis by activating the Nrf2/HO-1/NQO1 signaling pathway and facilitating Nrf2 nuclear translocation. Furthermore, RRFBs attenuated the BLM-induced changes in the gut microbiota; in particular, they decreased the abundance of the pathogenic bacterium Proteus and increased the abundance of the probiotics Ileibacterium and Dubosiella . Spearman correlation analysis revealed a strong association between oxidative stress inhibition and gut microbiota composition. These results indicated that RRFBs could exert lung-protective effects by inhibiting oxidative stress and alleviating intestinal disturbances.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
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IF 6.8
Siyu Chen,Yiwen Zhang,Shuang Liu,Juan Ma,Huiling Wang,Wei Chang,Hengfeng Li,Xiaochun Ding,Xuejun Pan,Xuewu Duan
DNA methylation is a key epigenetic regulator of gene expression, yet its role in pigment and ascorbic acid (AsA) metabolism in Rosa roxburghii remains unclear. This study examined the effects of 5-azacytidine (5-azaC), a DNA methyltransferase inhibitor, on postharvest fruit quality after cold storage. 5-azaC treatment reduced decay, delayed the onset of color change, preserved overall fruit brightness, maintained firmness, and suppressed respiration during cold storage. It inhibited chlorophyll a degradation and enhanced zeaxanthin, lutein, α-carotene, phytoene, and violaxanthin levels. 5-azaC enhanced AsA content, accompanied by increased activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), ascorbate oxidase (AO), and ascorbate peroxidase (APX). Integrated RNA-seq and WGBS analysis showed that 5-azaC induced carotenoid biosynthesis genes ( RrBCH, RrVDE, RrNSY, RrDXS and RrZE P), with RrVDE hypomethylated. RrMDHAR, RrDHAR, RrAO1, RrGR, RrGALUR, RrGalDH and RrIMPL2 were upregulated by 5-azaC, with reduced DNA methylation levels observed in RrAO1, RrGalDH and RrIMPL2 . Transcription factors RrAP2, RrMYB21, RrMYB44 and RrPosF21-like were induced by 5-azaC, with RrMYB21 hypomethylated. DNA demethylases ( RrROS1 and RrROS1-like ) were upregulated, while methyltransferases ( RrCMT-like, RrMET, RrMDR2 and RrDMR2-like ) were downregulated. Silencing RrAP2, RrMYB21, RrMYB44, RrPosF21-like and RrROS1-like reduced AsA content and downregulated AsA metabolism-related genes in R. roxburghii fruit . These findings provide preliminary evidence that 5-azaC may regulate pigment and AsA metabolism via epigenetic mechanisms during cold storage.
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Food Chemistry-X
·
2026
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IF 9.2
Xiaoli Zhou,Yan Chen,Shengyan Zhang,ShuangQin Zhang,Tailan Liu,Nian Liu,Yanxin Yang
Rosa roxburghi Tratt. (RRT) pomace is rich in insoluble dietary fiber (IDF) and polyphenols, but free polyphenols are susceptible to premature loss during digestion. This study constructed an IDF–polyphenol complex (IDF-PP) from RRT pomace via non-covalent adsorption and evaluated its gastrointestinal fate and in vitro colonic fermentation behavior. IDF-PP reduced premature phenolic loss during simulated upper gastrointestinal digestion and enabled a more gradual release during fermentation. Structural analyses showed fermentation-induced remodeling, generating a porous residual matrix while retaining part of the polysaccharide framework. Compared with free polyphenols and IDF, IDF-PP increased short-chain fatty acid production, especially butyrate, and was associated with higher relative abundance of Prevotella -centered Bacteroidetes taxa and Faecalibacterium , together with lower relative abundance of Proteobacteria . Fermentation products also showed enhanced antioxidant and α-glucosidase inhibitory activities. These findings suggest that RRT pomace-derived IDF-PP is a promising fermentation-responsive functional ingredient with potential to modulate gut microbial fermentation.
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Food & Function
·
2022
·Vol. 13
·期 17
·8880-8891
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IF 6.317
Juan Su,Xiong Fu,Qiang Huang,Guang Liu,Chao Li
The aim of this study was to elucidate the liberation and phytochemical profile of bound polyphenols present in dietary fiber (RPDF) isolated from Rosa roxburghii fruit pomace during in vitro-simulated digestion and colonic fermentation. The variation in the biological activity and prebiotic potential of the released polyphenols from RPDF was investigated. The results showed that the bound polyphenols were released from RPDF at a higher ratio during colonic fermentation than gastrointestinal digestion. The released polyphenols showed antioxidant activity and α-glucosidase inhibitory activity. The in vitro colonic fermentation assay showed that compared with dephenolized RPDF (DP-RPDF), RPDF exhibited a significantly stronger prebiotic effect by lowering the Firmicutes to Bacteroidetes (F/B) ratio at the phylum level, increasing the relative abundances of beneficial bacteria at the genus level, and improving the production of short chain fatty acids (SCFAs). These findings indicate that the bound polyphenols may act as important functional food ingredients that can contribute to the biological properties of RPDF. In addition, this study provides new insights into the high-value utilization of Rosa roxburghii fruit pomace, which will reduce the wastage of resources and greatly contribute to environmental protection.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2024
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IF 6.4
Juan Ma,Shuang Liu,Jing Zeng,Yiwen Zhang,Wei Chang,Zhengkun Meng,Yujia Zhou,Wene Zhang,Xiaochun Ding,Xuejun Pan,Xuewu Duan
Rosa roxburghii has a short and concentrated harvest period, during which rapid decay and quality deterioration at room temperature pose significant challenges to the supply chain. To address this, we applied methyl jasmonate (MeJA) treatment and stored the fruit at low temperatures. MeJA treatment effectively reduced decay, maintained fruit firmness and brightness, suppressed respiration, and decreased malondialdehyde content. Further analysis revealed that MeJA reduced hydrogen peroxide levels by boosting the activities and gene expressions of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). Additionally, MeJA upregulated the expression of disease resistance-related genes ( RrRGA3, RrPPO, RrCHIT, RrPRB1 , and RrRPM1 ). It also stimulated genes involved in the AsA synthesis and AsA-GSH cycle ( RrMIXO, RrAKRC9, RrDHAR , and RrGPX ), thereby increasing AsA content. Moreover, MeJA promoted the activities (PAL, C4H, and 4CL) and gene expressions ( RrPAL, Rr4CL, RrCSE, RrCCR, RrPGT, RrHCT , RrDFR and RrERF114 ) of phenylpropane metabolism, resulting in increased levels of L-phenylalanine, caffeic acid, phlorizin, and other phenolic acids and lignin content. Furthermore, MeJA induced the expression of genes related to JA biosynthesis ( RrAOC, RrOPR , and RrACX ), and abscisic acid synthesis ( RrNCED ). In conclusion, these findings suggest that MeJA treatment enhances disease resistance and preserves the postharvest quality of R. roxburghii , making it a promising preservation method for large-scale commercial application in fruit storage.
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FOOD CHEMISTRY
·
2026
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IF 10.4
Lina Luo,Junxi Zhou,Feiyan Yang,Lianfang Tian,Qian Chen,Yu Liu,Guofang Xie
Kombucha fermentation increases the bioactive components and reduces the sugar. • Komagataeibacter_saccharivorans and JC017_sp004296775 were identified as the key functional strains driving metabolism and flavor formation. • Kombucha fermentation promotes the production of beneficial components and reduces bitterness and volatile acids. • By integrating microbiome, metabolome and GC–MS data, the system reveals the dynamic association between microbial succession and metabolites during fermentation. Cili ( Rosa roxburghii Tratt) represents a promising novel substrate of kombucha. This study investigates the dynamic changes and interrelationships among bioactive compounds, flavor components, microbial communities, and metabolites during the fermentation of Cili kombucha. The results indicate that kombucha fermentation significantly increases the contents of organic acids, flavonoids, and polyphenols in Cili juice. An integrated multi-omics analysis identifies Komagataeibacter saccharivorans and JC017_sp004296775 as core functional strains that modulate metabolite biosynthesis and flavor regulation, which promoted the accumulation of beneficial metabolites, including D-tagatose, fumaric acid, indolelactic acid, and tryptophol. Concurrently, the fermentation process reduced the levels of raw material-derived volatile acids (acetic acid, octanoic acid, hexanoic acid, nonanoic acid) and bitter-tasting metabolites (quinic acid). This study verifies the feasibility of using Cili as a substrate for kombucha through comparative controls and provides a theoretical basis for targeted process optimization and functional product development. Download: Download high-res image (157KB) Download: Download full-size image
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JOURNAL OF ETHNOPHARMACOLOGY
·
2025
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IF 5.4
Jinlong Liu,Xingbo Bian,Xuefeng Bian,Jiahui Zhang,Zhengxin Li,Hanyang Liu,Jinying Wu,Xin Sun
Ethnopharmacological relevance Kaji-ichigoside F1 (KF1), the main active component of the Guizhou ethnic medicinal material Rosa roxburghii Tratt, is widely used in China due to its anti-inflammatory properties. However, the protective effects of KF1 against drug-induced liver injury and its potential mechanisms are not yet understood. Aim of the study We aimed to investigate the effects of KF1 on acute liver injury (ALI) and explore its underlying mechanisms, particularly its role in modulating the gut microbiota to inhibit ALI development. Materials and methods KF1 was prepared via 80 % ethanol extraction, silica gel column chromatography, and Sephadex LH-20 column chromatography. Mouse models of ALI were established using acetaminophen (APAP) treatment, with or without KF1 (5 and 10 mg/kg). 16S rRNA gene sequencing, metabolomics, and transcriptomics approaches were employed to explore the inhibitory effect of KF1 on ALI. Additionally, the role of the gut microbiota was investigated through antibiotic treatment and fecal microbiota transplantation experiments. Results Treatment with KF1 significantly altered the gut microbiota composition, notably increasing the abundance of the probiotic Akkermansia muciniphila ( A. muciniphila ). Furthermore, A. muciniphila enhanced the levels of beneficial metabolites, including inosine. Notably, inosine significantly suppressed inflammatory factors and improved APAP-induced ALI. Transcriptomic analysis revealed that inosine inhibited key signaling pathways, including MAPK, PI3K-AKT, JAK-STAT3, IL-17, TNF, and cytokine–cytokine receptor interactions. Importantly, the preventive effect of KF1 is dependent on microbial mechanisms. Conclusion KF1 protects against ALI by modulating the gut microbiota and associated metabolites, thereby promoting a more favorable state and inhibiting pro-inflammatory pathways.
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